Clonal Production of Cotton (Gossypium spp.) By T-Budding Method

Abstract

Clonal (vegetative) propagation is a technique used to increase productivity and grow large amount of seedlings in a short time from a single plant. The technique is especially useful to protect and increase pure lines. T-budding method used to propagate cotton plant clonally in this study. Experiment was conducted at the experimental fields of East Mediterranean Transitional Zone Agricultural Research Institute in 2010. Split plot design with three replications was used. Twelve meter plot length, 70x40 cm2 row spacing and suggested cultural practices for the region were applied. T-budding method in cotton was applied in July, August and September. Giza 45 (Gossypium barbadense L.) and Fantom (Gossypium hirsutum L.) were used as plant materials. Treatments were 1-[Fantom (root stock) + Fantom (scion)], 2-[Giza 45 (root stock) + Giza 45 (scion)], 3-[Giza 45 (root stock) + Fantom (scion)] and 4-[Fantom (root stock) + Giza 45 (scion)]. Graft success rates were significantly different for months and it was found higher within species than between species. The highest graft rates were obtained in July and August. With the application of this method in cotton, it is possible to maintain genotypes for years and propagate them asexually before seed formation using field and greenhouse facilities together. The method is also useful for the improvement of triploid genotypes obtained from breeding programs and important crosses having insufficient seeds.

Keywords

• Cotton
• T-budding
• cloning
• Gossypium hirsutum L.
• Gossypium barbadense L.

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