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Antibacterial Activity of Essential Oil and Extract of Origanum onites L. Against Bacterial Speck of Tomato and Tomato Bacterial Wilt Disease

Year 2019, , 30 - 35, 30.04.2019
https://doi.org/10.13002/jafag4549

Abstract

This study was carried out to determine antibacterial activity of Origanum onites L., essential oil and ethanol extract, against Pseudomonas syringae pv. tomato (Pst) and Clavibacter michiganensis subsp. michiganensis (Cmm). Plant material was collected from Tokat province in 2016. The study was carried under in vitro conditions and used King B medium. At concentrations, 10, 20, 30, 40 and 50% of the essential oil of O. onites was applied with method filter paper impregnation. The extract of O. onites was mixed with autoclaved King B medium to obtain final concentrations 1, 2, 3, 4 and 8%. And then 106 cell/ml of Pst and Cmm suspensions were placed on the medium containing the essential oil and extract. The media without essential oil and extract were used as the negative controls. Based on the results of the study, increase in the essential oil and extract concentration resulted in increase of the efficacy on pathogens. At concentration, of 50% essential oil of O. onites inhibited the growth of Pst and Cmm at a rate of 73% and 93%, respectively. At concentration of 8% extract of O. onites inhibited the growth of Pst and Cmm at a rate of 97% and 99%, respectively. In conclusion, it has been observed O. onites extract has higher antibacterial activity than essential oil.

References

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  • Aligiannis N, Kalpoutzakis E, Chinou IB (2001). Composition and antimicrobial activity of the essential oils of two Origanum species. Journal of Agriculture Food Chemistry, 49, 4168–4170.
  • Aysan Y, Mirik M, Çetinkaya-Yıldız R, Küsek M (2005). Pseudomonas syringae pv. tomato’nun Yayılmasında Tohum Kökenli İnokulumun Rolü. Türkiye II. Tohumculuk Kongresi, 353.
  • Aysan Y, Saygılı H (2008). Domates Bakteriyel Kara Leke Hastalığı, Domates Bakteriyel Benek Hastalığı, Bacterial Speck of Tomato, Pseudomonas syringae pv. tomato. Bitki Bakteri Hastalıkları Kitabı, (Saygılı H, Şahin F, Aysan Y), Meta Basım, İzmir, 123 s.
  • Bagamboula CF, Uyttendaele M, Debevere J (2004). ınhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and P-Cymene towards Shigella sonnei and S. flexneri. Food Microbiology 21: 33-43.
  • Baroffio CA, Siegfried W, Hilber UW (2003). Long-term monitoring for resistance of Botryotinia fuckeliana to anilinopyrimidine, phenylpyrrole, and hydroxyanilide fungicides in Switzerland. Plant Diseases, 87: 662-666.
  • Baydar H, Sağdıc O, Özkan G, Karadoğan T (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial ımportance in Turkey. Food Control 15: 169-172.
  • Beever RE, Brien HMR (1983). A Survey of resistance to the dicarboximide fungicides in Botrytis cinerea. New Zealand Journal of Agricultural Research 26: 391-400.
  • Ben HEN, Abdelkefi MM, Aissa RB, Chabouni MM (2001). Antibacterial screening of Origanum majorana L. oil from Tunisia. Journal of. Essenntial Oil Research, 13, 295-29.
  • Cosentino S, Tuberoso CIG, Pisano B, Satta M, Mascia V, Arzedi E, Palmas F (1999). In-vitro antimicrobial activity and chemical composition of sardinian Thymus essential oils. Lett Applied Microbiology 29: 130-135.
  • Çetinkaya-Yıldız R, Aysan Y (2008). Domates Bakteriyel Solgunluk Hastalığı, Tomato Bacterial Wilt Disease, Stem Canker, Bird’s Eye Spot, Clavibacter michiganensis subsp. michiganensis, Bitki Bakteri Hastalıkları Kitabı, (Saygılı H, Şahin F, Aysan Y), Meta Basım, İzmir, 49 s.
  • Daferera DJ, Ziogas BN, Polissiou MG (2003). The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp., and Clavibacter michiganensis subsp. michiganensis. Crop Protection 22: 39-44.
  • Demirci B, Tabanca N, Baser KHC (2002). Enantiomeric distribution of some monoterpenes in the essential oils of some Salvia species. Flavour and Fragrance Journal 17: 54-58.
  • EPPO (2017). European and Mediterranean Plant Protection Organization-IMI, https://gd.eppo.int/taxon/CORBMI/distribution
  • Farooqi AA, Sreeramu BS (2004). Cultivation of medicinal and aromatic crops. Universities Press, India. 465-470.
  • Gleason ML., Gitaitis RD., Ricker MD (1993). Recent progress in understanding and controlling bacterial canker of tomato in Eastern North America. Plant Disease, 77, 1069-1076.
  • Güner A, Özhatay N, Ekim T, Baser KHC (2000). Flora of Turkey and the east aegean ıslands (Vol. 11 (supplement-II)). Edinburgh: Edinburgh University Press.
  • Gökçe A, Whalon ME Çam H, Yanar Y, Demirtas I, Gören N (2007). Contact and residual toxicities of 30 plant extracts to colorado potato beetle larvae. Archives of Phytopathology and Plant Protection 149(2): 1-10
  • Ietswaart JH (1980). A Taxonomic revision of the genus Origanum (Labiatae). Ph.D Thesis. Leiden Botanical Series 4. Leiden University Press, The Hague.
  • Jones JB, Jones JP, Stall RE, Zitter TA (1991). Compendium of Tomato Diseases. APS Pres, USA.
  • Milijasevic S, Todorovic B, Rekanovi E, Potocnik I, Gavrilovic V (2009). Races and hosts of Pseudomanas syringae pv. tomato in Serbia. Archive Biological Science, 61: 93- 103.
  • Muller-Riebau F, Berger B, Yeğen O (1995). Chemical composition and fungitoxic properties to phytopathogenic fungi of essential oils of selected aromatic plants growing wild in Turkey. Journal of Agricultural and Food Chemistry, 43, 2262–2266.
  • Onaran A, Yılar M, Belguzar S, Bayan Y, Aksit H, 2(014). Antifungal and bioherbicidal properties of essential oils of Thymus fallax Fish &Mey., Origanum vulgare L. and Mentha dumetorum Schult. Asian Journal of Chemistry, 26(16): 5159-5164.
  • Panizi L, Flamini G, Cioni PL, Morelli I (1993). Compositionand antimicrobial properties of essential oils of four mediterranean Lamiaceae. Journal of Ethnopharmacology, 39, 167–170.
  • Ricker MD, Riedel RM (1993). Effect of secondary spread of Clavibacter michiganensis subsp. michiganensis on yield of northern processing tomatoes. Plant Diseases, 77, 364-366.
  • Sarer E, Pancalı S, Yıldız S (1996). Essential oil composition and antimicrobial activity of O. minutiflorum Schwarz et P.H. Davis. Ankara Eczacılık Fakültesi Dergisi 25:1.
  • Shehu A, Mohd AKR, Aniza A, Salwani I (2015). Antibacterial Activity and Antioxidant Capacity of Malaysian Tualang Honey. International Journal of Science and Research, 4 (4): 1758-1762.
  • Sheikh M, Safi-uddin A, Khan Z, Rizvi R, Mahmood I (2013). Antibacterial and antifungal potential of some medicinal plants against certain phytopathogenic microorganisms. Archives of Phytopathology and Plant Protection, 46(9), 1070-1080.
  • Sherf AF, Macnab AA (1986). Vegetable Diseases and Their Control, Second Edition. p. 398. A Wiley- Interscience Publication. John Wiley & Sons. New York.
  • Sivropoulou A, Papanikolaou E, Nikolaou C, Kokkini S (1996). Antimicrobial and cytotoxic activities of Origanum essential oils. Journal of Agricultural and Food Chemistry, 44:1202–1205.
  • Smitley DR, Mc Carter SM (1982). Spread of Pseudomonas syringae pv. tomato and role of epiphytic populations and environmental conditions in disease developments. Plant Disease 66: 713-717.
  • Soylu S, Yigitbas H, Soylu EM, Kurt S (2007). Antifungal effects of essential oils from oregano and fennelon Sclerotinia sclerotiorum Journal of Applied Microbiology.103:1021-1030.
  • Tabanca N, Kırımer N, Baser KHC (2001). The Composition of essential oils from two varieties of S. erytrantha var. erytrantha and cedretorum. Turkish Journal of Chemistry 25: 201-208.
  • Telci I, Bayram E, Yilmaz G, Avcı B (2006). Variability in essential oil composition of Turkish Basils (Ocimum basilicum L.). Biochemical Systematic Ecology, 34, 489-497.
  • Yanar Y, Belgüzar S, Telci I (2016). Origanum spp., Mentha spp. ve Lippia sp. Türlerine ait uçucu yağların Clavibacter michiganensis subsp. michiganensis ve Botrytis cinerea’ya Karşı Antimikrobiyal Etkisi. Turkish Journal of Weed Science. 19(1):18-25.
  • Yılar M, Bayan Y, Aksit H, Onaran A, Kadioglu I, Yanar Y (2013). Bioherbicidal effects of essential oils isolated from Thymus fallax, Mentha dumetorum Schult. and Origanum vulgare L. Asian J Chem, 25, 4807-4811.
  • Yiğit F (1993). Domateslerde erken yanıklık hastalığına karşı biyolojik savaşta Verticillium psalliotae Treschow‘nin etkinligi üzerinde arastırmalar. (Yüksek Lisans Tezi), Ege Üniversitesi, Bitki Koruma Anabilim Dalı, İZMİR.
  • Yonucu N (1997). Bitki ekstrakt ve kompostlarının Çukurova Bölgesinde sorun olan bazı fungal hastalıklara karşı antifungal özelliklerinin araştırılması. Yüksek Lisans Tezi, Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Ana bilim Dalı, Adana.
Year 2019, , 30 - 35, 30.04.2019
https://doi.org/10.13002/jafag4549

Abstract

References

  • Anonym (2017). https://www.organicfacts.net/health-benefits/essential-oils/health-benefits-of-oregano-essential-oil.html. (26.01.2017).
  • Aligiannis N, Kalpoutzakis E, Chinou IB (2001). Composition and antimicrobial activity of the essential oils of two Origanum species. Journal of Agriculture Food Chemistry, 49, 4168–4170.
  • Aysan Y, Mirik M, Çetinkaya-Yıldız R, Küsek M (2005). Pseudomonas syringae pv. tomato’nun Yayılmasında Tohum Kökenli İnokulumun Rolü. Türkiye II. Tohumculuk Kongresi, 353.
  • Aysan Y, Saygılı H (2008). Domates Bakteriyel Kara Leke Hastalığı, Domates Bakteriyel Benek Hastalığı, Bacterial Speck of Tomato, Pseudomonas syringae pv. tomato. Bitki Bakteri Hastalıkları Kitabı, (Saygılı H, Şahin F, Aysan Y), Meta Basım, İzmir, 123 s.
  • Bagamboula CF, Uyttendaele M, Debevere J (2004). ınhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and P-Cymene towards Shigella sonnei and S. flexneri. Food Microbiology 21: 33-43.
  • Baroffio CA, Siegfried W, Hilber UW (2003). Long-term monitoring for resistance of Botryotinia fuckeliana to anilinopyrimidine, phenylpyrrole, and hydroxyanilide fungicides in Switzerland. Plant Diseases, 87: 662-666.
  • Baydar H, Sağdıc O, Özkan G, Karadoğan T (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial ımportance in Turkey. Food Control 15: 169-172.
  • Beever RE, Brien HMR (1983). A Survey of resistance to the dicarboximide fungicides in Botrytis cinerea. New Zealand Journal of Agricultural Research 26: 391-400.
  • Ben HEN, Abdelkefi MM, Aissa RB, Chabouni MM (2001). Antibacterial screening of Origanum majorana L. oil from Tunisia. Journal of. Essenntial Oil Research, 13, 295-29.
  • Cosentino S, Tuberoso CIG, Pisano B, Satta M, Mascia V, Arzedi E, Palmas F (1999). In-vitro antimicrobial activity and chemical composition of sardinian Thymus essential oils. Lett Applied Microbiology 29: 130-135.
  • Çetinkaya-Yıldız R, Aysan Y (2008). Domates Bakteriyel Solgunluk Hastalığı, Tomato Bacterial Wilt Disease, Stem Canker, Bird’s Eye Spot, Clavibacter michiganensis subsp. michiganensis, Bitki Bakteri Hastalıkları Kitabı, (Saygılı H, Şahin F, Aysan Y), Meta Basım, İzmir, 49 s.
  • Daferera DJ, Ziogas BN, Polissiou MG (2003). The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp., and Clavibacter michiganensis subsp. michiganensis. Crop Protection 22: 39-44.
  • Demirci B, Tabanca N, Baser KHC (2002). Enantiomeric distribution of some monoterpenes in the essential oils of some Salvia species. Flavour and Fragrance Journal 17: 54-58.
  • EPPO (2017). European and Mediterranean Plant Protection Organization-IMI, https://gd.eppo.int/taxon/CORBMI/distribution
  • Farooqi AA, Sreeramu BS (2004). Cultivation of medicinal and aromatic crops. Universities Press, India. 465-470.
  • Gleason ML., Gitaitis RD., Ricker MD (1993). Recent progress in understanding and controlling bacterial canker of tomato in Eastern North America. Plant Disease, 77, 1069-1076.
  • Güner A, Özhatay N, Ekim T, Baser KHC (2000). Flora of Turkey and the east aegean ıslands (Vol. 11 (supplement-II)). Edinburgh: Edinburgh University Press.
  • Gökçe A, Whalon ME Çam H, Yanar Y, Demirtas I, Gören N (2007). Contact and residual toxicities of 30 plant extracts to colorado potato beetle larvae. Archives of Phytopathology and Plant Protection 149(2): 1-10
  • Ietswaart JH (1980). A Taxonomic revision of the genus Origanum (Labiatae). Ph.D Thesis. Leiden Botanical Series 4. Leiden University Press, The Hague.
  • Jones JB, Jones JP, Stall RE, Zitter TA (1991). Compendium of Tomato Diseases. APS Pres, USA.
  • Milijasevic S, Todorovic B, Rekanovi E, Potocnik I, Gavrilovic V (2009). Races and hosts of Pseudomanas syringae pv. tomato in Serbia. Archive Biological Science, 61: 93- 103.
  • Muller-Riebau F, Berger B, Yeğen O (1995). Chemical composition and fungitoxic properties to phytopathogenic fungi of essential oils of selected aromatic plants growing wild in Turkey. Journal of Agricultural and Food Chemistry, 43, 2262–2266.
  • Onaran A, Yılar M, Belguzar S, Bayan Y, Aksit H, 2(014). Antifungal and bioherbicidal properties of essential oils of Thymus fallax Fish &Mey., Origanum vulgare L. and Mentha dumetorum Schult. Asian Journal of Chemistry, 26(16): 5159-5164.
  • Panizi L, Flamini G, Cioni PL, Morelli I (1993). Compositionand antimicrobial properties of essential oils of four mediterranean Lamiaceae. Journal of Ethnopharmacology, 39, 167–170.
  • Ricker MD, Riedel RM (1993). Effect of secondary spread of Clavibacter michiganensis subsp. michiganensis on yield of northern processing tomatoes. Plant Diseases, 77, 364-366.
  • Sarer E, Pancalı S, Yıldız S (1996). Essential oil composition and antimicrobial activity of O. minutiflorum Schwarz et P.H. Davis. Ankara Eczacılık Fakültesi Dergisi 25:1.
  • Shehu A, Mohd AKR, Aniza A, Salwani I (2015). Antibacterial Activity and Antioxidant Capacity of Malaysian Tualang Honey. International Journal of Science and Research, 4 (4): 1758-1762.
  • Sheikh M, Safi-uddin A, Khan Z, Rizvi R, Mahmood I (2013). Antibacterial and antifungal potential of some medicinal plants against certain phytopathogenic microorganisms. Archives of Phytopathology and Plant Protection, 46(9), 1070-1080.
  • Sherf AF, Macnab AA (1986). Vegetable Diseases and Their Control, Second Edition. p. 398. A Wiley- Interscience Publication. John Wiley & Sons. New York.
  • Sivropoulou A, Papanikolaou E, Nikolaou C, Kokkini S (1996). Antimicrobial and cytotoxic activities of Origanum essential oils. Journal of Agricultural and Food Chemistry, 44:1202–1205.
  • Smitley DR, Mc Carter SM (1982). Spread of Pseudomonas syringae pv. tomato and role of epiphytic populations and environmental conditions in disease developments. Plant Disease 66: 713-717.
  • Soylu S, Yigitbas H, Soylu EM, Kurt S (2007). Antifungal effects of essential oils from oregano and fennelon Sclerotinia sclerotiorum Journal of Applied Microbiology.103:1021-1030.
  • Tabanca N, Kırımer N, Baser KHC (2001). The Composition of essential oils from two varieties of S. erytrantha var. erytrantha and cedretorum. Turkish Journal of Chemistry 25: 201-208.
  • Telci I, Bayram E, Yilmaz G, Avcı B (2006). Variability in essential oil composition of Turkish Basils (Ocimum basilicum L.). Biochemical Systematic Ecology, 34, 489-497.
  • Yanar Y, Belgüzar S, Telci I (2016). Origanum spp., Mentha spp. ve Lippia sp. Türlerine ait uçucu yağların Clavibacter michiganensis subsp. michiganensis ve Botrytis cinerea’ya Karşı Antimikrobiyal Etkisi. Turkish Journal of Weed Science. 19(1):18-25.
  • Yılar M, Bayan Y, Aksit H, Onaran A, Kadioglu I, Yanar Y (2013). Bioherbicidal effects of essential oils isolated from Thymus fallax, Mentha dumetorum Schult. and Origanum vulgare L. Asian J Chem, 25, 4807-4811.
  • Yiğit F (1993). Domateslerde erken yanıklık hastalığına karşı biyolojik savaşta Verticillium psalliotae Treschow‘nin etkinligi üzerinde arastırmalar. (Yüksek Lisans Tezi), Ege Üniversitesi, Bitki Koruma Anabilim Dalı, İZMİR.
  • Yonucu N (1997). Bitki ekstrakt ve kompostlarının Çukurova Bölgesinde sorun olan bazı fungal hastalıklara karşı antifungal özelliklerinin araştırılması. Yüksek Lisans Tezi, Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Ana bilim Dalı, Adana.
There are 38 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Ayşe Yeşilayer This is me

Sabriye Belgüzar This is me

Hande Nur Aslan This is me

Publication Date April 30, 2019
Published in Issue Year 2019

Cite

APA Yeşilayer, A., Belgüzar, S., & Aslan, H. N. (2019). Antibacterial Activity of Essential Oil and Extract of Origanum onites L. Against Bacterial Speck of Tomato and Tomato Bacterial Wilt Disease. Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG), 36(1), 30-35. https://doi.org/10.13002/jafag4549