Drought Tolerance of Some Wine Grape Cultivars Under In Vitro Conditions

Year 2019, , 145 - 152, 31.08.2019

Neval Topçu Altıncı Rüstem Cangi

https://doi.org/10.13002/jafag4633

Cited By: 1

Abstract

This study was conducted to determine the drought tolerance of 6 different economically important wine grape cultivars (‘Sultani Seedless’, ‘Çalkarası’, ‘Emir’, ‘Boğazkere’, ‘Öküzgözü’, ‘Narince’) of Turkey under in vitro conditions. Drought stress was induced on in vitro-grown explants by 3 different PEG (8000) (poly ethylene glycol) doses (2, 4 and 6 %). Plants were subjected to drought stress for 6 weeks and plant fresh weight, dry weight, shoot length, number of shoots, number of leaves, electrolyte leakage, relative water content, proline content and lipid peroxidation (MDA) were determined. Being more distinctive at higher doses, PEG treatments yielded significant decreases in fresh weight, dry weight, shoot length, number of shoots and number of leaves. As compared to the control, PEG treatments also yielded greater electrolyte leakage in all cultivars. All three PEG concentrations decreased relative water content of all cultivars. Proline content of explants increased with increasing PEG doses. While plant response to PEG treatments varied with the cultivars in 2 % PEG treatments, significant increases were observed in MDA content of all cultivars at higher doses (4 and 6 %).

Keywords

PEG, in vitro Screening, Proline, MDA

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