Multiple Shoot and Callus Formation in Different Explants of The Medicinally Important Peganum harmala L.

Year 2020, , 77 - 83, 31.08.2020

Burcu Çetin Halil İbrahim Koçak

https://doi.org/10.13002/jafag4451

Abstract

In this study, the micropropagation potential of the medicinally important Peganum harmala L. plant containing valuable metabolites has been examined. For this purpose, hypocotyl and shoot tips isolated from P. harmala plants were applied different concentrations and combinations of plant growth regulators of benzyl amino purine (BAP), naphthalene acetic acid (NAA), and indole acetic acid (IAA). Best callus growth were obtained in hypocotyl explants in Murashige and Skoog (MS) medium that contained 1 mg L-1 NAA and 1 mg L-1 BAP. Best results concerning shoot count per explant (5.42±0.313 count) and shoot length (2.95±0.06 cm) were determined in MS medium which contained 1.5 mg L-1 BAP. IBA applications were performed in order to root the obtained shoots. The rooted plants were transferred to the soil with a success rate of 72%.

Keywords

Peganum harmala L., plant growth regulator, plant tissue culture, micropropagation

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