Seed and Pollen Transmission of Tomato spotted wilt orthotospovirus (TSWV) in Pepper

Year 2024, , 186 - 192, 31.12.2024

Yunus Emre Uslu , Sevdiye Yorgancı

https://doi.org/10.55507/gopzfd.1547338

Abstract

Orthotospovirus tomatomaculae also known by Tomato spotted wilt orthotospovirus (TSWV) is a harmful pathogen that significantly reduces crop production and quality. TSWV can infect approximately 1,000 plant species in 84 different plant families, such as tomato, pepper, lettuce, tobacco, and various types of wild plants, belonging to the Solanaceae family. TSWV is a member of the Orthotospovirus genus and is an isometric-enveloped particle. TSWV can be transmitted mechanically, seed-borne, and by nine species of thrips belonging to the genera Thrips, Scirtothrips,Frankliniella.In this study, it was determined that TSWV could be transmitted by seeds, but no transmission by pollen was detected. RT-PCR and visual assessment data demonstrate the seed transmission ability of TSWV in peppers. TSWV was detected in infected pepper seeds (seed and seed coat). Additionally, TSWV was detected in different organs of the pepper plant. TSWV was detected in the endocarp, mesocarp, exocarp, pedicel, and placenta of pepper fruit and sepal, petal, pistil, and stamen of pepper flower. After the germination of infected pepper seeds, TSWV was detected with specific primers by RT-PCR in the cotyledons and true leaves, but no symptoms were observed. These data provided information about the localization of TSWV in pepper plants and provided further evidence of TSWV seed transmission ability.

Keywords

Tomato spotted wilt orthotospovirus, seed transmission, pollen transmission, localization of virus, pepper plant.

Biberde Domates Lekeli Solgunluk Virüsünün Tohum ve Polen ile Taşınması

Abstract

Orthotospovirus tomatomaculae olarakta bilinen Domates lekeli solgunluk virüsü (TSWV), ürün verimini ve kalitesini önemli ölçüde azaltan zararlı bir patojendir. TSWV, domates, biber, marul, tütün ve Solanaceae familyasına ait çeşitli yabani bitkiler gibi 84 farklı bitki familyasındaki yaklaşık 1.000 bitki türünü enfekte edebilir. TSWV, Orthotospovirüs cinsinin bir üyesi olup izometrik zarflı bir partiküldür. TSWV, mekanik olarak, tohum yoluyla ve Thrips, Scirtothrips ve Frankliniella cinslerine ait dokuz trips türü tarafından bulaşabilir. Bu çalışmada, TSWV'nin tohumlarla taşınabildiği, ancak polen yoluyla taşınma kabiliyetinin olmadığı belirlenmiştir. RT-PCR ve görsel değerlendirme verileri, TSWV'nin biberlerde tohumla taşınma kabiliyetini göstermektedir. Enfekteli biberlerden toplanan tohumlarda (tohum ve tohum kabuğu) TSWV tespit edilmiştir. Ek olarak, enfekteli, biber meyvesinin endokarp, mezokarp, ekzokarp, pedicel ve plasentasında ve biber çiçeğinin çanak yaprağı, taç yaprağı, pistili ve erkek organında TSWV saptanmıştır. Enfekteli biber tohumlarının çimlenmesinden sonra, TSWV kotiledonlarda ve gerçek yapraklarda spesifik primerler kullanılarak RT-PCR ile tespit edilmiş, ancak hiçbir simptom gözlemlenmemiştir. Bu veriler, TSWV'nin biber bitkisinde ki lokalizasyonu hakkında bilgi sağlamış ve TSWV tohum ile taşınabildiğine dair daha fazla kanıt sağlamıştır.

Keywords

Biber bitkisi, domates lekeli solgunluk virüsü, polen taşınması, tohum taşınması, virüs lokalizasyonu

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