Clonal Production of Cotton (Gossypium spp.) By T-Budding Method

Year 2017, Volume: 34 Issue: 3, 100 - 104, 29.12.2017

Ramazan Şadet Güvercin

https://doi.org/10.13002/jafag4288

Abstract

Clonal (vegetative) propagation is a technique used to increase productivity and grow large amount of seedlings in a short time from a single plant. The technique is especially useful to protect and increase pure lines. T-budding method used to propagate cotton plant clonally in this study. Experiment was conducted at the experimental fields of East Mediterranean Transitional Zone Agricultural Research Institute in 2010. Split plot design with three replications was used. Twelve meter plot length, 70x40 cm2 row spacing and suggested cultural practices for the region were applied. T-budding method in cotton was applied in July, August and September. Giza 45 (Gossypium barbadense L.) and Fantom (Gossypium hirsutum L.) were used as plant materials. Treatments were 1-[Fantom (root stock) + Fantom (scion)], 2-[Giza 45 (root stock) + Giza 45 (scion)], 3-[Giza 45 (root stock) + Fantom (scion)] and 4-[Fantom (root stock) + Giza 45 (scion)]. Graft success rates were significantly different for months and it was found higher within species than between species. The highest graft rates were obtained in July and August. With the application of this method in cotton, it is possible to maintain genotypes for years and propagate them asexually before seed formation using field and greenhouse facilities together. The method is also useful for the improvement of triploid genotypes obtained from breeding programs and important crosses having insufficient seeds.

Keywords

Cotton, T-budding, cloning, Gossypium hirsutum L., Gossypium barbadense L.

References

• Bozbek T, Özbek N, Sezener V, Erdoğan O, Yavaş İ and Ünay A (2008). Natural crossing and isolation distance between cotton genotypes in Turkey. Scientia Agricole, 65(3): 314-317.
• http://www.ces.ncsu.edu/depts/hort/hil/grafting.html (Accessed to web: 18.01.2010)
• Mert M and Bayraktar NA (1994). Research on the Determination of Cross Pollination Ratio in Cotton (Gossypium hirsutum L.) under Amik Plain Conditions. National Field Crops Congress, Proceedings, 2: 227-230. (in Turkish)
• Oğlakçı M, Efe L, Çiçek B and Çıra AA (2000). Research on the Determination of Natural Crossing Percentage of Cotton (Gossypium hirsutum L.) in Kahramanmaraş Conditions. The Inter-Regional Cooperative Research Network on Cotton, 33-35, Adana. (in Turkish)
• Rea HE (1931). Grafting experiments with cotton. Plant Physiology, 6 (1): 193–196.
• Sen İ, Oğlakçı M, Bölek Y, Çiçek B, Kısakürek N and Aydın S (2004). Assessing the out-crossing ratio, isolation distance and pollinator insects in cotton (Gossypium hirsutum L.). Asian Journal of Plant Science, 3(6): 724-727.
• Ünay A, Kaynak MA, Gürel A, Akdemir H and Serter E (1998). The Extent of Cross Pollination for Seed Production in Cotton. Agricultural Congress of Aegean Region, 197-202, Aydın Turkey. (in Turkish)
• Xanthopoloulos FP and Kechagia UE (2000). Natural crossing in cotton. The Inter-Regional Cooperative Research Network on Cotton, 20-24 September 2000, 27-32, Adana Turkey.
• Yolcu S (1995). The Determination of Natural Cotton Pollination Percentage and Pollinator Insect Species under Harran Plain Conditions of Turkey. Harran University. MSc Thesis, Şanlıurfa Turkey. (in Turkish)