Old Home x Farmingdale 333 Armut Anacının in Vitro Köklenmesi Üzerine Oksin ve Polivinil Alkol Uygulamalarının Etkileri

Year 2018, Volume: 35 Issue: Ek Sayı, 47 - 53, 01.10.2018

Shabnam Alizadeh Gülüstan Polat Hatice Dumanoğlu

https://doi.org/10.13002/jafag4482

Cited By: 1

Abstract

Bu çalışmada, Old Home x Farmingdale 333 (OHxF 333) (Pyruscommunis L.) armut anacında mikro çeliklerin in vitro köklenmesi üzerine PVA’nın (polivinil alkol), IBA (indolbütirik asit) ve IAA (indolasetikasit)’nın etkileri araştırılmıştır. PVA denemesinde (1. deneme), aseptik koşullarda, hızlı daldırma yöntemiyle 200 ppm IBA uygulanmış mikro çelikler, 0, 1, 2 ve 3 gl-1 PVA ilave edilmiş, büyümeyi düzenleyici madde içermeyen, makro element düzeyi ½ olan MS (Murashige ve Skoog) temel besin ortamı üzerinde kültüre alınmıştır. Oksin denemesinde (2. deneme) ise IBA ve IAA’nın 0, 12, 24, 48, 120 ve 240 ppm dozlarının aseptik koşullarda yavaş daldırma yöntemiyle (1 saat) uygulandığı mikro çelikler, PVA içermeyen aynı temel besin ortamına dikilmiştir. Kültürler bir hafta karanlık, beş hafta 16 saat aydınlık koşullarda tutulmuştur. Çalışmanın sonucunda, hızlı daldırma yöntemiyle 200 ppm IBA uygulanmış mikro çeliklerde PVA (1, 2 ve 3 g l-1)’nın invitro köklenme oranlarını önemli düzeyde artırdığı (%75-100) belirlenmiştir. Köklenme düzeyi (ortalama 2.51), ortalama kök sayısı (ortalama 3.54 adet) ve kök uzunluğu (ortalama 17.0 mm) bakımından uygulamalar arasında istatistiksel anlamda önemli bir farklılık ortaya çıkmamıştır. Oksin denemesinde ise en yüksek köklenme oranları IAA’nın 48, 120, 240 ppm ve IBA’nın 12, 24, 120 ppm uygulamalarında %50.0 ile %83.3 arasında kaydedilmiştir.

Keywords

Pyrus, mikro çoğaltım, in vitro, köklenme, PVA

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