Effect of Media, Stress Conditions and Genotype on Androgenetic Performance of Pepper

Year 2020, Volume: 37 Issue: 1, 23 - 29, 30.04.2020

Sevtap Doksöz Boncukçu Murat Emre Çelik Naif Geboloğlu

https://doi.org/10.13002/jafag4627

Abstract

This study was conducted with the purpose of investigation of the effects of genotype, nutrient media, pre-treatments and incubation conditions on haploid plant development on some three-lobed pepper genotypes. İstek F1, Köylüm F1, Üçburun F2 and Tokat local genotype were used as donor plants. DDV (Dumas de Vaulx et al., 1981) and MS (Murashige and Skoog, 1962) were used as nutrient media. Furthermore, 0.01 mg L-1 Kinetin + 0.01 mg L-1 2,4-D + 0.03 mg L-1 Vitamin B12 were added to the media. Flower buds were held at 4 oC for 24 hours. Low-temperature treatment was not applied on flower buds of control plants. After anthers were placed in the nutrient media, they were held at 9 oC and 35 oC for 8 hours in the darkness. Afterwards, anthers were held at 25±2 oC for 16 h day / 8 h night for 4 days and transferred to regeneration media. All genotypes that were used in the experiment gave a response to the anther culture more or less. The highest embryo development was obtained from Tokat genotype. Embryo development rates varied between 0.25% and 31.00% by treatments. While embryo development rate was 3.9% in MS medium, it was 9.33% in DDV medium. Moreover, while embryo development rate was 6.7% on anthers that were exposed to cold pre-treatment, it was 6.4% in the control treatment. Embryo development rate in 9 oC and 35oC incubation treatments were 7.60% and 5.50% respectively.

Keywords

Anther culture, DDV, genotype, incubation, MS, pre-treatment

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