Research Article
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Effect of Media, Stress Conditions and Genotype on Androgenetic Performance of Pepper

Year 2020, Volume: 37 Issue: 1, 23 - 29, 30.04.2020
https://doi.org/10.13002/jafag4627

Abstract

This study was conducted with the purpose of investigation of the effects of genotype, nutrient media, pre-treatments and incubation conditions on haploid plant development on some three-lobed pepper genotypes. İstek F1, Köylüm F1, Üçburun F2 and Tokat local genotype were used as donor plants. DDV (Dumas de Vaulx et al., 1981) and MS (Murashige and Skoog, 1962) were used as nutrient media. Furthermore, 0.01 mg L-1 Kinetin + 0.01 mg L-1 2,4-D + 0.03 mg L-1 Vitamin B12 were added to the media. Flower buds were held at 4 oC for 24 hours. Low-temperature treatment was not applied on flower buds of control plants. After anthers were placed in the nutrient media, they were held at 9 oC and 35 oC for 8 hours in the darkness. Afterwards, anthers were held at 25±2 oC for 16 h day / 8 h night for 4 days and transferred to regeneration media. All genotypes that were used in the experiment gave a response to the anther culture more or less. The highest embryo development was obtained from Tokat genotype. Embryo development rates varied between 0.25% and 31.00% by treatments. While embryo development rate was 3.9% in MS medium, it was 9.33% in DDV medium. Moreover, while embryo development rate was 6.7% on anthers that were exposed to cold pre-treatment, it was 6.4% in the control treatment. Embryo development rate in 9 oC and 35oC incubation treatments were 7.60% and 5.50% respectively.

References

  • Abak K (1983). Study on the anther culture in vitro of pepper (Capsicum annuum). Capsicum Newslett, 2, 72-73.
  • Abak K (1984). Biberde (Capsicum annuum L.) anter kültürü yolu ile haploid bitki elde etme üzerinde araştırmalar. Ankara Üniversitesi. Ziraat Fak Yıllığı. 1–4, 155–163 (in Turkish).
  • Achar PN (2002). A study of factors affecting embriyo yields from anther culture of cabbage. Plant Cell Tissue Organ Cult. 69(2), 183-188.
  • Alremi F, Taskın H, Sonmez K, Buyukalaca S, Ellialtıoglu S (2013). Biber (Capsicum annuum L.)’de Genotip ve Besin Ortamının Anter Kültürüne Etkileri DOI: 10.30910/turkjans.160702.
  • Cardoza V, Stewart JCN (2004). Brassica biotechnology: progress in cellular and molecular biology. In Vitro Cel Dev Biol Plant. 40(6), 542-551.
  • Chambonnet D (1988). Production of haploid pepper plants. Bulletin interne de la station d‘Amelioration des Plantes Maraicheres d‘Avignon-Montfavet, France. 1-10.
  • Chlyah A, Taarji H (1984). Proc. Int. Symp. Plant tissue and cell culture application to crop improvement. 24–29 Sept.
  • Ciner D, Tıpırdamaz R (2002). The effects of cold treatment and charcoal on the in vitro androgenesis of pepper (Capsicum annuum L.). Turkish Journal of Botany. 26,131-139.
  • Comlekcioglu N, Büyükalaca S, Abak K (2001). Effect of silver nitrate on haploid embryo induction by anther culture in pepper (Capsicum annuum). XIth EUCARPIA Meeeting on Genetics and Breeding of Capsicum & Eggplant, 2001, Antalya-Turkey
  • Dumas De Vaulx R, Chambonnet D, Sibi M (1981). Culture in vitro d‘antheres de piment (Capsicum annuum L.): amelioration des taux d‘obtention de plantes chez differents genotypes par de traitments A+35°C. Agronomie. 11, 859.
  • Dunwell JM (2010). Haploids in flowering plants: origins and exploitation. Plant Biotechnology Journal. 8, 377–424.
  • Ellialtıoglu S, Sari N, Abak K (2000). Haploid Bitki Üretimi. (Bitki Biyoteknolojisi Cilt:I, Ed: Babaoğlu, M., Özcan, S., Gürel, E.) 40 s.
  • Ellialtıoglu S, Kaplan F, Abak K (2001). The effect of carrot extract and activated charcoal on the androgenesis of pepper. In Proceedings of XIth EUCARPIA Meeting on Genetics and Breeding of Capsicum & Eggplant. pp. 9-13
  • Ellialtıoglu S, Tıpırdamaz R (1997). Soğuk uygulamaları ve aktif kömürün patlıcan ve biberde in vitro androgenesis üzerine etkileri, TÜBİTAK-TOGTAG 87 No‘lu proje Sonuç Raporu, 70 s. Ankara.
  • Ercan N, Boyacı F, Ayar F (2001). Biberde (Capsicum annuum L.) anter kültürü yoluyla haploid bitki eldesi üzerine farklı besin ortamlarının etkisi. GAP II. Tarım Kongresi, 24-26 Ekim, Şanlıurfa, Cilt 1: 121-128.
  • Foroughi-Wehr B, Wenzel G (1993). Andro- and parthenogenesis. In: Plant Breeding. Principles and Prospects. Hayvard, M.D., Bosemark, N.O., Ramagosa, I. (eds.)., Chapman & Hall, London, pp. 261-277.
  • George L, Narayanaswamy S (1973). Haploid Capsicum through experimental androgenesis. Protoplasma 78, 467-470.
  • Koleva-GudevaL R, Spasenoski M, Trajkova F (2007). Somatic embryogenesis in pepper anther culture: The effect of incubation treatments and different media. Scientia Horticulturae. 111(2), 114-119.
  • Kothari SL, Joshi A, Kachhwaha S, Ochoa-Alejo N (2010). Chilli peppers—a review on tissue culture and transgenesis. Biotechnology advances. 28(1), 35-48.
  • Kristiansen K, Andersen SB (1993). Effects of donor plant temperature, photoperiod, and age on anther culture response of Capsicum annuum L. Euphytica. 67,105– 109.
  • Linsmaier EM, Skoog F (1965). Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18,100±127.
  • Malhotra K, Maheshwari SC (1977). Enhancement by Cold Treatment of Pollen Embryoid Development in Petunia hybrida Zeitschrift für Pflanzenphysiologie. 85(2), 177-180 https://doi.org/10.1016/S0044-328X(77)80293-6.
  • Matsubara S, Hu K, Murakami K (1992). Embryoid and callus formation from polen grains of eggplant and pepper by anther culture. Journal of the Japanese Society for Horticultural Science 61(1), 69–77 https://doi.org/10.2503/jjshs.61.69
  • Mityko J, Andrasfalvy A, Csıllery G, Farı M (1995). Anther culture response in different genotypes and F1 hybrids of pepper (Capsicum annuum L.). Plant Breeding, 114,78-80.
  • Mityko J, Fari M (1997). Problems and results of doubled haploid plant production in pepper (Capsicum annuum L.) via anther and microspore culture. Acta Hortic. 447,281-287 DOI: 10.17660/ActaHortic.1997.447.57
  • Morrison RA, Koning, RE, Evans DA (1986). Anther culture of ınterspesific hybrid of Capsicum. J. Plant Physiol. 126,1-9.
  • Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassy with tobacco tissue cultures. Physiol. Plant. 15, 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • Nitsch JP, Nitsch C (1969). Haploids Plants from Pgrains. Science 163, 85-87.
  • Nitsch C (1974). La culture de pollen isole sur milieu synthetique. C. R. Acad. Sci. Ser. D., 278, 1031-1034.
  • Ochoa-Alejo N, Ramirez-Malagon R (2001). In vitro chili pepper biotechnology. In Vitro Cel Dev Biol Plant. 37(6), 701-729.
  • Rodeva V, Grozeva S, Todorova V (2006). In vitro answer of Bulgarian pepper (Capsicum annuum L.) varieties. Genetica (Serbia) 38(2),129–136.
  • Sayılır A, Ozzambak ME (2000). Brokkolide anter kültürü denemeleri. III. Sebze Tarımı Sempozyumu, Isparta, 218-222.
  • Sayılır A, Ozzambak ME (2002). Biber anter kültüründe uygun tomurcuk büyüklüğü tespiti ile besin ortamları karışımlarının ve soğuk uygulama sürelerinin embriyo verimine etkisi üzerine bir araştırma. IV. Sebze Tarımı Sempozyumu, Bursa.
  • Smith RH (2013). Plant tissue culture: techniques and experiments. Academic Press.
  • Sunderland N, Roberts M (1979). Cold-pretremant of excised flower buds in float culture of tobacco anther. Ann. Bot. 43 (4),405-414 https://doi.org/10.1093/oxfordjournals.aob.a085650.
  • Taşkın H (2005). Different applications to improve the embryo quality on stimulation of embryo by anther culture in some pepper genotypes. Ziraat Fakültesi Yüksek Lisans, Sayfa Sayısı: 79.
  • Terzioglu S, Ellialtıoglu S, Abak K (2000). İnkübasyon koşullarının biber anter kültüründe embriyo oluşumu üzerine etkisi. III. Sebze Tarımı Sempozyumu.11-13 Eylül, Isparta. s:233-238.
  • Touraev A, Heberle-Bors E (2003). Anther and microspore culture in tobacco. In Doubled Haploid Production in Crop Plants (pp. 223-228). Springer Netherlands.
  • Wang YY, Sun, CS, Wang CC, Chien NJ (1973). The induction of polen plantlets of Triticale and Capsicum annuum from anther culture. Sci. Sin, 16, 147-151.
  • Zhang E, Ou C, Xu Z, Cheng Y (2005). Factors affecting embryoid induction and formation of cabbage anthers in culture. Acta Botanica Boreali-Occidentalia Sinica. 26(11), 2372.
Year 2020, Volume: 37 Issue: 1, 23 - 29, 30.04.2020
https://doi.org/10.13002/jafag4627

Abstract

References

  • Abak K (1983). Study on the anther culture in vitro of pepper (Capsicum annuum). Capsicum Newslett, 2, 72-73.
  • Abak K (1984). Biberde (Capsicum annuum L.) anter kültürü yolu ile haploid bitki elde etme üzerinde araştırmalar. Ankara Üniversitesi. Ziraat Fak Yıllığı. 1–4, 155–163 (in Turkish).
  • Achar PN (2002). A study of factors affecting embriyo yields from anther culture of cabbage. Plant Cell Tissue Organ Cult. 69(2), 183-188.
  • Alremi F, Taskın H, Sonmez K, Buyukalaca S, Ellialtıoglu S (2013). Biber (Capsicum annuum L.)’de Genotip ve Besin Ortamının Anter Kültürüne Etkileri DOI: 10.30910/turkjans.160702.
  • Cardoza V, Stewart JCN (2004). Brassica biotechnology: progress in cellular and molecular biology. In Vitro Cel Dev Biol Plant. 40(6), 542-551.
  • Chambonnet D (1988). Production of haploid pepper plants. Bulletin interne de la station d‘Amelioration des Plantes Maraicheres d‘Avignon-Montfavet, France. 1-10.
  • Chlyah A, Taarji H (1984). Proc. Int. Symp. Plant tissue and cell culture application to crop improvement. 24–29 Sept.
  • Ciner D, Tıpırdamaz R (2002). The effects of cold treatment and charcoal on the in vitro androgenesis of pepper (Capsicum annuum L.). Turkish Journal of Botany. 26,131-139.
  • Comlekcioglu N, Büyükalaca S, Abak K (2001). Effect of silver nitrate on haploid embryo induction by anther culture in pepper (Capsicum annuum). XIth EUCARPIA Meeeting on Genetics and Breeding of Capsicum & Eggplant, 2001, Antalya-Turkey
  • Dumas De Vaulx R, Chambonnet D, Sibi M (1981). Culture in vitro d‘antheres de piment (Capsicum annuum L.): amelioration des taux d‘obtention de plantes chez differents genotypes par de traitments A+35°C. Agronomie. 11, 859.
  • Dunwell JM (2010). Haploids in flowering plants: origins and exploitation. Plant Biotechnology Journal. 8, 377–424.
  • Ellialtıoglu S, Sari N, Abak K (2000). Haploid Bitki Üretimi. (Bitki Biyoteknolojisi Cilt:I, Ed: Babaoğlu, M., Özcan, S., Gürel, E.) 40 s.
  • Ellialtıoglu S, Kaplan F, Abak K (2001). The effect of carrot extract and activated charcoal on the androgenesis of pepper. In Proceedings of XIth EUCARPIA Meeting on Genetics and Breeding of Capsicum & Eggplant. pp. 9-13
  • Ellialtıoglu S, Tıpırdamaz R (1997). Soğuk uygulamaları ve aktif kömürün patlıcan ve biberde in vitro androgenesis üzerine etkileri, TÜBİTAK-TOGTAG 87 No‘lu proje Sonuç Raporu, 70 s. Ankara.
  • Ercan N, Boyacı F, Ayar F (2001). Biberde (Capsicum annuum L.) anter kültürü yoluyla haploid bitki eldesi üzerine farklı besin ortamlarının etkisi. GAP II. Tarım Kongresi, 24-26 Ekim, Şanlıurfa, Cilt 1: 121-128.
  • Foroughi-Wehr B, Wenzel G (1993). Andro- and parthenogenesis. In: Plant Breeding. Principles and Prospects. Hayvard, M.D., Bosemark, N.O., Ramagosa, I. (eds.)., Chapman & Hall, London, pp. 261-277.
  • George L, Narayanaswamy S (1973). Haploid Capsicum through experimental androgenesis. Protoplasma 78, 467-470.
  • Koleva-GudevaL R, Spasenoski M, Trajkova F (2007). Somatic embryogenesis in pepper anther culture: The effect of incubation treatments and different media. Scientia Horticulturae. 111(2), 114-119.
  • Kothari SL, Joshi A, Kachhwaha S, Ochoa-Alejo N (2010). Chilli peppers—a review on tissue culture and transgenesis. Biotechnology advances. 28(1), 35-48.
  • Kristiansen K, Andersen SB (1993). Effects of donor plant temperature, photoperiod, and age on anther culture response of Capsicum annuum L. Euphytica. 67,105– 109.
  • Linsmaier EM, Skoog F (1965). Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18,100±127.
  • Malhotra K, Maheshwari SC (1977). Enhancement by Cold Treatment of Pollen Embryoid Development in Petunia hybrida Zeitschrift für Pflanzenphysiologie. 85(2), 177-180 https://doi.org/10.1016/S0044-328X(77)80293-6.
  • Matsubara S, Hu K, Murakami K (1992). Embryoid and callus formation from polen grains of eggplant and pepper by anther culture. Journal of the Japanese Society for Horticultural Science 61(1), 69–77 https://doi.org/10.2503/jjshs.61.69
  • Mityko J, Andrasfalvy A, Csıllery G, Farı M (1995). Anther culture response in different genotypes and F1 hybrids of pepper (Capsicum annuum L.). Plant Breeding, 114,78-80.
  • Mityko J, Fari M (1997). Problems and results of doubled haploid plant production in pepper (Capsicum annuum L.) via anther and microspore culture. Acta Hortic. 447,281-287 DOI: 10.17660/ActaHortic.1997.447.57
  • Morrison RA, Koning, RE, Evans DA (1986). Anther culture of ınterspesific hybrid of Capsicum. J. Plant Physiol. 126,1-9.
  • Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassy with tobacco tissue cultures. Physiol. Plant. 15, 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • Nitsch JP, Nitsch C (1969). Haploids Plants from Pgrains. Science 163, 85-87.
  • Nitsch C (1974). La culture de pollen isole sur milieu synthetique. C. R. Acad. Sci. Ser. D., 278, 1031-1034.
  • Ochoa-Alejo N, Ramirez-Malagon R (2001). In vitro chili pepper biotechnology. In Vitro Cel Dev Biol Plant. 37(6), 701-729.
  • Rodeva V, Grozeva S, Todorova V (2006). In vitro answer of Bulgarian pepper (Capsicum annuum L.) varieties. Genetica (Serbia) 38(2),129–136.
  • Sayılır A, Ozzambak ME (2000). Brokkolide anter kültürü denemeleri. III. Sebze Tarımı Sempozyumu, Isparta, 218-222.
  • Sayılır A, Ozzambak ME (2002). Biber anter kültüründe uygun tomurcuk büyüklüğü tespiti ile besin ortamları karışımlarının ve soğuk uygulama sürelerinin embriyo verimine etkisi üzerine bir araştırma. IV. Sebze Tarımı Sempozyumu, Bursa.
  • Smith RH (2013). Plant tissue culture: techniques and experiments. Academic Press.
  • Sunderland N, Roberts M (1979). Cold-pretremant of excised flower buds in float culture of tobacco anther. Ann. Bot. 43 (4),405-414 https://doi.org/10.1093/oxfordjournals.aob.a085650.
  • Taşkın H (2005). Different applications to improve the embryo quality on stimulation of embryo by anther culture in some pepper genotypes. Ziraat Fakültesi Yüksek Lisans, Sayfa Sayısı: 79.
  • Terzioglu S, Ellialtıoglu S, Abak K (2000). İnkübasyon koşullarının biber anter kültüründe embriyo oluşumu üzerine etkisi. III. Sebze Tarımı Sempozyumu.11-13 Eylül, Isparta. s:233-238.
  • Touraev A, Heberle-Bors E (2003). Anther and microspore culture in tobacco. In Doubled Haploid Production in Crop Plants (pp. 223-228). Springer Netherlands.
  • Wang YY, Sun, CS, Wang CC, Chien NJ (1973). The induction of polen plantlets of Triticale and Capsicum annuum from anther culture. Sci. Sin, 16, 147-151.
  • Zhang E, Ou C, Xu Z, Cheng Y (2005). Factors affecting embryoid induction and formation of cabbage anthers in culture. Acta Botanica Boreali-Occidentalia Sinica. 26(11), 2372.
There are 40 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Sevtap Doksöz Boncukçu This is me

Murat Emre Çelik This is me

Naif Geboloğlu This is me

Publication Date April 30, 2020
Published in Issue Year 2020 Volume: 37 Issue: 1

Cite

APA Doksöz Boncukçu, S., Çelik, M. E., & Geboloğlu, N. (2020). Effect of Media, Stress Conditions and Genotype on Androgenetic Performance of Pepper. Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG), 37(1), 23-29. https://doi.org/10.13002/jafag4627