Determination of an SSR Marker Set to Distinguish Genotypes of Different Tobacco Classes

Year 2020, Volume: 37 Issue: 2, 102 - 108, 31.08.2020

Ibrahim Saygılı Ahmet Kınay Nejdet Kandemır

https://doi.org/10.13002/jafag4677

Cited By: 3

Abstract

Tobacco is produced as cultivated old varieties or local varieties in Turkey. Seed exchanges among farmers who produce their own seeds lead to different names for the same genotypes in different regions or the same name for different genotypes over time. Thus, an efficient cultivar identification system is required in tobaccos grown in Turkey. This study was carried out to determine a marker set to differentiate different tobacco classes grown in Turkey. A total of 14 genotypes from different tobacco classes, eight local varieties and six registered cultivars, were screened using 21 simple sequence repeats (SSR) markers. Seventeen SSR markers produced polymorphic bands while four markers were monomorphic. Average allele number of the markers was 3.38 and PIC values of the markers ranged from 0.000 to 0.741. In dendrogram established using the marker data, genotypes were separated in four distinct cluster. Clusters were made of Virginia or semi-oriental, tombac, oriental and rustica type genotypes. Genetic fingerprinting analyses grouped the genotypes of similar use classes together. According to the results obtained a marker set of eight SSR markers could efficiently and reliably differentiated the tobacco genotypes of different classes.

Keywords

Fingerprint analysis, Microsatellite, Nicotiana tabacum, Polymorphism

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