Acetamiprid Resistance in the Green Peach Aphid Myzuspersicae(Sulzer) (Hemiptera: Aphididae): Selection, Cross-Resistance, Biochemical and Molecular Resistance Mechanisms

Year 2022, Volume: 39 Issue: 3, 136 - 142, 31.12.2022

Gizem Berber , Berke Demirci , Umut Toprak , Emre İnak , Sibel Yorulmaz

https://doi.org/10.55507/gopzfd.1144369

Cited By: 2

Abstract

Myzus persicae(Sulzer) (Hemiptera: Aphididae) is a polyphagous pest that causes significant losses in many crops. In the present study, the biochemical and molecular mechanism of acetamiprid resistance in a laboratory-selected M.persicae population of which the resistance ratios reached 57.5-fold were investigated. This study was conducted in the Isparta University of Applied Sciences, Agriculture Faculty, Department of Plant Protection in 2018 and 2020. Synergism, biochemical and molecular assays showed the absence of increased P450 activity in selected population. In addition, no point mutation in nicotinic acetylcholine receptor (nAChR), the target-site of neonicotinoids including acetamiprid, was detected in the selected population. These results suggests that high level of acetamiprid resistance might be developed via the mechanisms other than well-known mechanisms, such as increased P450 activity and target-site mutations. The population selected with acetamiprid showed decreased susceptibility to imidacloprid, sulfoxaflor, beta-cyfluthrin, and tau-fluvanite ranging from 1.54 to 4.76. Nonetheless, more studies are needed to support cross-resistance by M. persicae populations having different genetic backgrounds.

Keywords

acetamiprid, CYP6CY3, monooxygenease, Myzus persicae, resistance

Yeşil Şeftali Yaprakbiti Myzus persicae'de (Sulzer) (Hemiptera: Aphididae) Acetamiprid Direnci: Seleksiyon, Çapraz Direnç, Biyokimyasal ve Moleküler Direnç Mekanizmaları

Abstract

Myzus persicae (Sulzer) (Hemiptera: Aphididae), birçok üründe önemli kayıplara neden olan polifag bir zararlıdır.Bu çalışmada, laboratuarda seleksiyon baskısı sonucunda direnç oranı 57.5 kata ulaşan bir M. persicae popülasyonunda acetamipriddirencinin biyokimyasal ve moleküler mekanizması araştırılmıştır. Çalışma 2018-2020 yılları arasında Isparta Uygulamalı Bilimler Üniversitesi, Ziraat Fakültesi, Bitki Koruma bölümünde yürütülmüştür. Sinerjistik, biyokimyasal ve moleküler çalışmalar, seleksiyon popülasyonunda artan P450 aktivitesinin olmadığını göstermiştir. Buna ilaveten, seleksiyon popülasyonunda, acetamiprid dahil neonikotinoidlerin hedef bölgesi olan nikotinik asetilkolin reseptöründe (nAChR) herhangi bir nokta mutasyonu belirlenmemiştir. Bu sonuçlar, seleksiyon popülasyonunda artan P450 aktivitesi ve hedef bölge mutasyonları dışındaki mekanizmalar yoluyla yüksek düzeyde asetamiprid direncinin geliştirilebileceğini düşündürmektedir. Acetamipridile selekte edilen popülasyonda, imidacloprid, sulfoxaflor, beta-cyfluthrin ve tau-fluvanite karşı 1.54 ila 4.76 kat arasında duyarlılık azalması belirlenmiştir. Bununla birlikte, farklı genetik geçmişlere sahip M. persicae popülasyonlarında çapraz direncin belirlemek için daha fazla çalışmaya ihtiyaç bulunmaktadır

Keywords

acetamiprid,, CYP6CY3,, monooksijenaz,, Myzus persicae, direnç

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