Hypericum perforatum Releasing From Silver Sulphadiozine Loaded Sodium Alginate Membranes

Abstract

This study presents that the controlled release of Hypericum perforatum (HP) from biofilm formation of sodium alginate (NaAlg). Its oil was incorporated in sodium alginate biofilm as a potential therapeutic agent. HP belongs from the family of Hypericaceae. HP is traditionally used for wound healing in Turkey and has medical importance. In addition, bacterial resistance of HP was investigated for against Staphylococcus aureus. Silver Sulfadiazine (SSD) was added in different concentration (0.5-2.0 w/v) to prepare the antibacterial biofilms. The addition of antibiotics and HP into the biofilm will ensure the delivery of all the necessary substances locally in the wound healing process. Biofilms were prepared by solvent casting method in different oil concentration (0.25-2.0 % v/v). The surface properties of biofilms were investigated by using Scanning Electron Microscopy (SEM), drug release profiles were studied at pH 5.5 and bacterial resistance of HP was investigated for against Staphylococcus aureus. As a result of our study, the new generation hydrogel wound dressing will, heal wounds effectively and quickly especially protecting the burns from microorganisms.

Keywords

• Hypericum Perforatum,, hydrogel,, controlled release

References

1. Abdollahzadeh, E., Rezaei, M., Hosseini, H. (2014). Antibacterial activity of plant essential oils and extracts: the role of thyme essential oil, nisin and their combination to control Listeria monocytogenes inoculated in minced fish meat, Food Control, 35, 177-183, doi: 10.1016/j.foodcont.2013.07.004.
2. Adoor, S.G.; Prathab, B.L.; Manjeshwar, S; Aminabhavi, T.M. (2007) Mixed matrix membranes of sodium alginate and poly(vinyl alcohol) for pervaporation dehydration of isopropanol at different temperatures. Polymer. 48, 5417-5430.
3. Aoyagi S, Onishi H, Machida Y. (2007) Novel chitosan wound dressing loaded with minocycline for the treatment of severe burn wounds. Int J Pharm. Feb 7;330(1-2):138-45. E pub 2006 Sep 17.
4. Avato, P., Raffo, F., Guglielmi, G., Vitali, C., & Rosaro, A. (2004). Extracts from St. John’s wort and their anitimicrobial activity, Phytoterapy Research, 18, 230-232, doi: 10.1002/ptr.1430
5. Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods-a review. International Journal of Food Microbiology. 94, 223-253, doi: 10.1016/j.ijfoodmicro.2004.03.022.
6. Güneş, S., Tıhmınoğlu, F. (2017). Hypericum perforatum incorporated chitosan films as potential bioactive wound dressing material. International Journal of Biological Macromolecules, 102, 993-943, doi:10.1016/j.ijbiomac.2017.04.080
7. Jayakumar, R., Prabaharan, M, Kumar Sudheesh, P.T., Nair, S.V., Tamura, H. (2011). Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnology Advances. 29, 322-337, doi: 10.1016/biotechadv.2011.01.005.
8. Khan A, Khan M, Subhan F, Gilani AH. (2010) Antispasmodic, bronchodilator and blood pressure lowering properties of Hypericum oblongifolium–possible mechanism of action. Phytotherapy Research. 24(7):1027–32.

9. Kim JO, Choi JY, Park JK, Kim JH, Jin SG, Chang SW, Li DX, Hwang MR, Woo JS, Kim JA, Lyoo WS, Yong CS, Choi HG, (2008) Development of clindamycin-loaded wound dressing with polyvinyl alcohol and sodium alginate. Biological Pharmaceutical Bulletin Dec;31(12):2277-82.
10. Noppakundilograt, S; Piboon, P; Graisuwan, W; Nuisin, R; (2015) Kiatkamjornwong, S. Encapsulated eucalyptus oil in ionically cross-linked alginate microcapsules and its controlled release. Carbohydrate Polymer, 131, 23-33.
11. Paul, W., Sharma, C. (2004). Chitosan and alginate wound dressing: a short review. Trends in Biomaterials and Artificial Organs. 18, 18-23, doi: 10.1016/j.ijpharm.2012.05.044
12. Pawar, H.V., Tetteh, J., Boateng, J.S. (2013). Preperation, optimisation and characterisation of novel wound healing film dressing loaded with streptomycin and diclofenac. Colloids Surfaces B Biointerfaces. 102, 102-110, doi: 10.1016/j.colsurrfb.2012.08.014
13. Peng, Y., Li, Y. (2014). Combined effects of two kinds of essential oils on physical, mechanical and structural properties of chitosan films, Food Hydrocolloids, 36, 287-293, doi:10.1016/j.foodhyd.2013.10.013.
14. Rassu, G; Salis, A; Porcu, E.P.; Giunchedi, P; Roldo, M; Gavini, E. (2016) Composite chitosan/alginate hydrogel for controlled release of deferoxamine: A system to potentially treat iron dysregulation diseases. Carbohydrate Polymer, 136, 1338-1347.
15. Raziq, N., Saeed, M., Shahis, M., Muhammad, N., Khan, H. & Gul, F. (2016). Pharmacological basis for the use of Hypericum oblongifolium as a medical plant in the management of pain, inflammation and pyrexia. BMC Complementary Medicine and Therapies. 16, 41, doi:10.1186/s12906-016-1018-z.
16. Saddiqe, Z., Naeem, I., Maimoona, A. (2010). A review of the antibacterial activity of Hypericum perforatum L. Journal of Ethnopharmacology, 131, 511-521, doi: 10.1016/j.jep.2010.07.034
17. Thu, H.E., Zulfakar, M.H., Ng, S.F. (2012). Alginate based bilayer hydrocolloid films as potential slow-release modern wound dressing. International Journal of Pharmaceutics. 434. 375-383. doi: 10.1016/j.ijpharm.2012.05.044.