Bioinformatics analysis and investigation of the effects of different fertilizer treatments on the expression profile of Cannabinoid Synthase gene in Cannabis sativa

Year 2025, Volume: 1 Issue: 2, 79 - 88, 31.10.2025

Alireza Mirzaei , Jamshid Fooladi

Abstract

Cannabis sativa is one of the most important plants producing cannabidiol, and it has a wide application in medicine. This study was conducted to investigate the effects of nitrogen, phosphorus, potassium, iron, manganese, and magnesium treatments on the expression profile of Cannabinoid Synthase gene in Cannabis sativa clones. The results of this study showed that fertilization has a direct effect on expression of Cannabinoid Synthase gene. In addition, potassium treatment stimulated the expression of Cannabinoid Synthase gene more than the other treatments studied. The results of the bioinformatics analysis showed that clones 4 and 5 were in the same category and were 55% similar to each other. In addition, the three clones studied in Cannabis sativa were in the same category compared to other related genes in other species. Also, protein blast analysis of Cannabinoid Synthase gene in different species showed that Morus alba, Arabidopsis thaliana, Phleum pretense, and Cynodon dactylon species have more structural similarity than the corresponding Cannabinoid Synthase gene in Glycine max. On the other hand, the study of the expression profile of Cannabinoid Synthase gene in Arabidopsis thaliana showed that the expression level of this gene was constant in the leaf and that fewer expression changes were seen in the Cannabinoid Synthase gene compared to other organs. In general, this research provided an accurate estimate of the expression analysis of the Cannabinoid Synthase gene in Cannabis sativa and other reference species, which provides researchers with a useful perspective on the extraction of cannabidiol from Cannabis sativa.

Keywords

Cannabinoid , gene expression , Phylogenetic analysis , fertilization , Cannabis sativa

Biyoinformatik analiz ve farklı gübre uygulamalarının Cannabis sativa'da Kannabinoid Sentaz geninin ekspresyon profili üzerindeki etkilerinin araştırılması

Abstract

Cannabis sativa, kannabidiol üreten en önemli bitkilerden biri olup tıpta geniş bir uygulamaya sahiptir. Bu çalışma, azot, fosfor, potasyum, demir, manganez ve magnezyum uygulamalarının Cannabis sativa klonlarında Kannabinoid Sentaz geninin ifade profilindeki değişikliklere olan etkilerini araştırmak için yürütülmüştür. Bu çalışmanın sonuçları, döllenmenin kontrol durumuna kıyasla Kannabinoid Sentaz geninin ifadesini artırmada doğrudan bir etkiye sahip olduğunu göstermiştir. Bu sonuçlara göre Kannabinoid Sentaz geninin yapraktaki ve klon 4'teki ifade düzeyinin diğer ilgili klonlardan daha yüksek olduğunu belirlenmiştir. Ek olarak, potasyum uygulaması Kannabinoid Sentaz geninin ifadesini çalışılan diğer uygulamalardan daha fazla uyarmıştır. Bu çalışmanın biyoenformatik analiz sonuçları, 4 ve 5 numaralı klonların aynı kategoride olduğunu ve birbirlerine %55 oranında benzediğini göstermektedir. Ayrıca, Cannabis sativa'da çalışılan üç klon, diğer türlerdeki diğer ilgili genlerle karşılaştırıldığında aynı kategoride olduğu görülmektedir. Farklı türlerde Kannabinoid Sentaz geninin protein blast analizi, Morus alba, Arabidopsis thaliana, Phleum pretense ve Cynodon dactylon türlerinin Glycine max'taki karşılık gelen Kannabinoid Sentaz geninden daha fazla yapısal benzerliğe sahip olduğunu göstermektdir. Öte yandan, Arabidopsis thaliana'da Kannabinoid Sentaz geninin ifade profilinin incelenmesi, bu genin ifade düzeyinin yaprakta sabit olduğunu ve diğer organlarla karşılaştırıldığında Kannabinoid Sentaz geninde daha az ifade değişikliğinin görüldüğünü göstermektdir. Genel olarak bu araştırma, Cannabis sativa ve diğer referans türlerinde Kanabinoid Sentaz geninin ifade analizinin doğru bir tahminini sunarak araştırmacılara Cannabis sativa'dan kannabidiolün çıkarılması konusunda yararlı bir bakış açısı sağladığı sonucuna varılmıştır.

Keywords

Kanabinoid , gen ifadesi , filogenetik analiz , döllenme , Cannabis sativa

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