Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota

Taha Ceylani; Sevcan Kabayer; Vesila Yıldırım; Enes Esmertaş; Hikmet Taner Teker

doi:10.5281/zenodo.17474573

EN TR

Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota

Abstract

Aging is accompanied by profound alterations in gut microbiota composition, often leading to a dysbiotic state characterized by reduced abundance of short-chain fatty acid (SCFA) producers. Intermittent fasting (IF) and probiotics (Prb) have emerged as dietary strategies with potential to counteract these changes. Twenty-four-month-old Sprague Dawley rats were assigned to control (Cnt), intermittent fasting (IF), probiotic (Prb), or combined IF+Prb groups. Cecal contents were subjected to 16S rDNA sequencing, and the relative abundance of key SCFA-producing taxa was determined. Correlation analyses (Spearman and Pearson) and heatmap visualizations were applied to examine ecological relationships and group-specific shifts. The control group displayed a dysbiotic profile with diminished representation of butyrate- and propionate-producing taxa. In contrast, IF, Prb, and IF+Prb interventions consistently promoted the enrichment of core SCFA producers such as Anaerostipes hadrus, Roseburia intestinalis, Butyrivibrio fibrisolvens, and Akkermansia muciniphila. Additional taxa including Intestinimonas butyriciproducens, Coprococcus catus, and Clostridium hylemonae also showed increased abundances under intervention conditions. Correlation analyses revealed coordinated ecological restructuring, supporting restoration of microbial networks linked to SCFA production. These findings demonstrate that intermittent fasting and probiotics, independently or in combination, mitigate age-associated dysbiosis by selectively enhancing SCFA-producing bacterial communities. Such interventions may represent promising nutritional strategies to restore gut microbial balance and improve host metabolic health in aging.

Keywords

Intermittent Fasting, Probiotics, Gut Microbiota, Short-Chain Fatty Acids, Dysbiosis, Aging

Yaşlı Bağırsak Mikrobiyotasında Aralıklı Oruç ve Probiyotiklerin Kombinasyonuyla SCFA Üreten Bakterilerin Modülasyonu

Abstract

Yaşlanma, bağırsak mikrobiyotası bileşiminde derin değişikliklere eşlik eder ve bu genellikle kısa zincirli yağ asidi (SCFA) üreticilerinin azalmış bolluğu ile karakterize disbiyotik bir duruma yol açar. Aralıklı oruç (IF) ve probiyotikler (Prb), bu değişikliklere karşı koyma potansiyeline sahip diyet stratejileri olarak ortaya çıkmıştır. Yirmi dört aylık Sprague Dawley sıçanları kontrol (Cnt), aralıklı oruç (IF), probiyotik (Prb) veya kombine IF + Prb gruplarına atandı. Kör bağırsak içerikleri 16S rDNA dizilemesine tabi tutuldu ve önemli SCFA üreten taksonların göreceli bolluğu belirlendi. Ekolojik ilişkileri ve grup-özgü değişimleri incelemek için korelasyon analizleri (Spearman ve Pearson) ve ısı haritası görselleştirmeleri uygulandı. Kontrol grubu, bütirat ve propiyonat üreten taksonların azalmış temsiliyle disbiyotik bir profil gösterdi. Buna karşılık, IF, Prb ve IF+Prb müdahaleleri, Anaerostipes hadrus, Roseburia intestinalis, Butyrivibrio fibrisolvens ve Akkermansia muciniphila gibi temel SCFA üreticilerinin zenginleştirilmesini sürekli olarak desteklemiştir. Intestinimonas butyriciproducens, Coprococcus catus ve Clostridium hylemonae dahil ek taksonlar da müdahale koşulları altında artan bolluk göstermiştir. Korelasyon analizleri, SCFA üretimiyle bağlantılı mikrobiyal ağların restorasyonunu destekleyen koordineli ekolojik yeniden yapılanmayı ortaya koymuştur. Bu bulgular, aralıklı oruç ve probiyotiklerin, bağımsız olarak veya birlikte, SCFA üreten bakteri topluluklarını seçici olarak artırarak yaşa bağlı disbiyozu hafiflettiğini göstermektedir. Bu tür müdahaleler, bağırsak mikrobiyal dengesini yeniden sağlamak ve yaşlanmada konakçı metabolik sağlığını iyileştirmek için umut verici beslenme stratejileri sunabilir.

Keywords

Aralıklı Oruç, Probiyotikler, Bağırsak Mikrobiyotası, Kısa Zincirli Yağ Asitleri, Disbiyoz, Yaşlanma

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Details

Primary Language

English

Subjects

Neural Engineering

Journal Section

Research Article

Authors

Taha Ceylani
0000-0002-3041-6010
Türkiye

Sevcan Kabayer
0009-0006-8826-6787
Türkiye

Vesila Yıldırım
0009-0007-7900-8791
Türkiye

Enes Esmertaş
0009-0007-3379-0706
Türkiye

Hikmet Taner Teker ^*
0000-0002-6621-3071
Türkiye

Publication Date

October 30, 2025

Submission Date

August 23, 2025

Acceptance Date

October 13, 2025

Published in Issue

Year 2025 Volume: 2 Number: 2

DOI

https://doi.org/10.5281/zenodo.17474573

IZ

https://izlik.org/JA56GJ62JL

APA

Ceylani, T., Kabayer, S., Yıldırım, V., Esmertaş, E., & Teker, H. T. (2025). Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota. Hendese Teknik Bilimler Ve Mühendislik Dergisi, 2(2), 78-83. https://doi.org/10.5281/zenodo.17474573

AMA

1.Ceylani T, Kabayer S, Yıldırım V, Esmertaş E, Teker HT. Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota. HENDESE. 2025;2(2):78-83. doi:10.5281/zenodo.17474573

Chicago

Ceylani, Taha, Sevcan Kabayer, Vesila Yıldırım, Enes Esmertaş, and Hikmet Taner Teker. 2025. “Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota”. Hendese Teknik Bilimler Ve Mühendislik Dergisi 2 (2): 78-83. https://doi.org/10.5281/zenodo.17474573.

EndNote

Ceylani T, Kabayer S, Yıldırım V, Esmertaş E, Teker HT (October 1, 2025) Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota. Hendese Teknik Bilimler ve Mühendislik Dergisi 2 2 78–83.

IEEE

[1]T. Ceylani, S. Kabayer, V. Yıldırım, E. Esmertaş, and H. T. Teker, “Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota”, HENDESE, vol. 2, no. 2, pp. 78–83, Oct. 2025, doi: 10.5281/zenodo.17474573.

ISNAD

Ceylani, Taha - Kabayer, Sevcan - Yıldırım, Vesila - Esmertaş, Enes - Teker, Hikmet Taner. “Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota”. Hendese Teknik Bilimler ve Mühendislik Dergisi 2/2 (October 1, 2025): 78-83. https://doi.org/10.5281/zenodo.17474573.

JAMA

1.Ceylani T, Kabayer S, Yıldırım V, Esmertaş E, Teker HT. Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota. HENDESE. 2025;2:78–83.

MLA

Ceylani, Taha, et al. “Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota”. Hendese Teknik Bilimler Ve Mühendislik Dergisi, vol. 2, no. 2, Oct. 2025, pp. 78-83, doi:10.5281/zenodo.17474573.

Vancouver

1.Taha Ceylani, Sevcan Kabayer, Vesila Yıldırım, Enes Esmertaş, Hikmet Taner Teker. Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota. HENDESE. 2025 Oct. 1;2(2):78-83. doi:10.5281/zenodo.17474573

Modulation of SCFA-Producing Bacteria by the Combination of Intermittent Fasting and Probiotics in the Aging Gut Microbiota

Abstract

Keywords

Yaşlı Bağırsak Mikrobiyotasında Aralıklı Oruç ve Probiyotiklerin Kombinasyonuyla SCFA Üreten Bakterilerin Modülasyonu

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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