Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine

Year 2023, , 77 - 82, 31.03.2023

Aykut Arif Topçu

https://doi.org/10.17350/HJSE19030000294

Abstract

The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the poly(hydroxyethyl methacrylate) [p(HEMA)] cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution.

The maximum adsorption capacity (Qmax) of lysozyme was found as 108.9 mg/g and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin (BSA). Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.

Keywords

lysozyme, adsorption, molecular imprinting, p(HEMA), dopamine, cryogel.

Thanks

Dr. Aykut Arif Topçu thanks Prof. Dr. Adil Denizli for his kind help and valuable contributions.

References

• Topcu, AA, Kılıc, S, Özgür, E, Türkmen, D, Denizli, A. Inspirations of biomimetic affinity ligands: a review. ACS Omega 7 (2022) 32897-32907.
• Bereli et al., Molecular imprinting technology for biomimetic assemblies. Hacettepe J. Biol. & Chem., 48 (5) (2020) 575-601.
• Haupt, K Peer reviewed: molecularly imprinted polymers: the next generation. Anal. Chem, 75 (17) (2003) 376 A-383 A.
• Dong, C, Shi, H, Han, Y, Yang, Y, Wang, R, Men, J. Molecularly imprinted polymers by the surface imprinting technique. Eur. Polym. J, 145 (2021) 110231.
• Dena, ASA, Ali, AM, El-Sherbiny IM. Surface-imprinted polymers (SIPs): Advanced Materials for bio-recognition. J. Nanotech. & Adv. Mat, 8 (1) (2020) 1-19.
• Tan, CJ, Tong, YW. Molecularly imprinted beads by surface imprinting. Anal. Bioanal. Chem, 389 (2007) 369-376.
• Saylan, Y, Denizli, A. Supermacroporous Composite cryogels in biomedical applications. GELS 5(2) (2019) 1-20.
• Perçin, I, Sağlar, E, Yavuz, H, Aksöz, E, Denizli, A. Poly(hydroxyethyl methacrylate) based affinity cryogel for plasmid DNA purification. Int. J. Biol. Macromol, 48 (4) (2011) 577-82.
• Mól, PCG etl al. Development of an affinity cryogel for one step purification of lysozyme from chicken egg white. J. Chromatog. B 1044-1045 (2017) 17-23.
• Mourâo, CA, Marcuz, C, Haupt, K, Bueno, SMA. Polyacrylamide-alginate (PAAm-Alg) and phospho-L-tyrosine-linked PAAm-Alg monolithic cryogels: Purification of IgG from human serum. J. Chromatog. B 1129 (2019) 121783.
• Razavi, M, Qiao, Y, Thakor, AS. Three-dimensional cryogels for biomedical applications. J. Biomed. Mater. Res, 107A (2019) 2736-2755.
• Alkan, H, Bereli, N, Baysal, Z, Denizli, A. Antibody purification with protein a attached supermacroporous poly(hydroxyethyl methacrylate) cryogel. Biochem. Eng. J, 45 (2009) 201-208.
• Çorman, EM. Poly-l-lysine modified cryogels for efficient bilirubin removal from human plasma. Colloids Surf. B, 167 (2018) 291-198.
• Özgür, E, Bereli, N, Türkmen, D, Ünal, S, Denizli, A. PHEMA cryogel for in-vitro removal of anti-dsDNA antibodies from SLE plasma. Mater. Sci. Eng. C, 31 (2011) 915-920.
• Kavoshchian, M, Üzek, R, Uyanık, SA, Şenel, S, Denizli, A. HSA immobilized novel polymeric matrix as an alternative sorbent in hemoperfusion columns for bilirubin removal. React. Funct. Polym, 96 (2015) 25-31.
• Hu, M, Ge, W, Liu, X, Zhu, Y. Preconcentration and determination of zearalenone in corn oil by a one-step prepared polydopamine-based magnetic molecularly imprinted polymer (mip) with high-Performance liquid chromatography with fluorescence (hplc- fld) detection. Anal. Lett, 5 (3) (2022) 343-354.
• Ma, Y et al. Polydopamine-coated magnetic nanoparticles for enrichment and direct detection of small molecule pollutants coupled with maldi-tof-ms. ACS Appl. Mater. Interfaces 5 (3) (2013) 1024-1030.
• Lee, H, Dellatore, SM, Miller, WM, Messersmith, PB. Mussel inspired surface chemistry for multifunctional coatings. Science 318 (2007) 426-430.
• Zheng, P et al. Facile preparation of polydopamine-coated imprinted polymers on the surface of SiO2 for estrone capture in milk samples. J. Sep. Sci, 41 (2018) 2585-2594.
• Zhang, M, Zhang, X, He, X, Chen, L, Zhang, Y. A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein. Nanoscale 4 (2012) 3141-3147.
• Li, Y et al. A graphene nanoplatelet-polydopamine molecularly imprinted biosensor for Ultratrace creatinine detection. Biosens. Bioelectron, 216 (2022) 114638.
• Tan, F, Liu, M, Ren, S. Preparation of polydopamine-coated graphene oxide/Fe3O4 imprinted nanoparticles for selective removal of fluoroquinolone antibiotics in water. Sci. Rep, 7 (2017) 5725.
• Topçu, AA, Bereli, N, Albayrak, İ, Denizli, A. Creatinine imprinted poly(hydroxyethyl methacrylate) based cryogel cartridges. J. Macromol. Sci. A, 5 (8) (2017) 495-501.
• Yilmaz, F, Köse, K, Sarı, MM, Demirel, G, Uzun, L, Denizli, A. Bioinspired surface modification of poly(2-hydroxyethyl methacrylate) based microbeads via oxidative polymerization of dopamine. Colloids Surf. B, 109 (2013) 176-182.
• Zabihi, S, Bakhshpour, Çalışır, M, Topçu, AA, Denizli, A. Preparation of molecular imprinted injectable polymeric micro cryogels for control release of mitomycin. Polym Bull. (2022).
• Luo, H, Gu, C, Zheng, W, Dai, F, Wang, X, Zheng, Z. Facile synthesis of novel size-controlled antibacterial hybrid spheres using silver nanoparticles loaded with poly-dopamine spheres. RSC Adv, 5 (2015) 13470-13477.
• Liu, Q, Yu, B, Ye, W, Zhou, F. Highly selective uptake and release of charged molecules by pH-responsive polydopamine microcapsules. Macromol. Biosci, 11 (9) (2011) 1227-1234.
• Gündüz, F, Bayrak, B. Biosorption of malachite green from an aqueous solution using pomegranate peel: equilibrium modelling, kinetic and thermodynamic studies. J. Mol. Liq, 243 (2017) 790-798.
• Raghuwanshi, VS, Yu, Breandan, Browne, C, Garnier, G. Reversible pH Responsive Bovine Serum Albumin Hydrogel Sponge Nanolayer. Front. Bioeng. Biotechnol, 8 (2020) 1-20.
• Xu, W, Dai, Q, Wang, Y, Hu, X, Xu, P, Ni, P, Meng, J. Creating magnetic ionic-liquid molecularly imprinted polymers for selective extraction of lysozyme. RSC Adv, 8 (2018) 21850-21856.
• Xu, K, Wang, Y, Wei, X, Chen, J, Xu, P, Zhou, Y. Preparation of magnetic molecularly imprinted polymers based on a deep eutectic solvent as the functional monomer for specific recognition of lysozyme. Microchim. Acta 185 (146) (2018)1-7.
• Xu, K, Dai, Q, Wang, Y, Chen, K, Qian, Y. Construction of ionic liquid-crosslinked magnetic surface-imprinted polymers for selective recognition of lysozyme. Microchem. J, 179 (2022) 107552.