Use of Heat-killed Aspergillus ochraceus NRRL 3174 Discs as Biosorbent for petroleum Removal

Year 2023, , 219 - 228, 30.09.2023

Nermin Hande Avcioglu , Sezen Bılen Ozyurek , Işıl Seyis Bilkay

https://doi.org/10.17350/HJSE19030000310

Abstract

The purpose of this work was to evaluate the petroleum sorption capacity of heat-killed fungal discs obtained from Aspergillus ochraceus strain. Effect of various parameters such as biosorbent dose (0.5g-2.5g/100mL), petroleum concentration (0.5-5%), pH (4.0-8.0), contact time (1-12h) and re-usability of biosorbent (1-6) were investigated. Accordingly, the highest biosorption capacity was obtained with 1% petroleum concentration, 1.5 g/100mL heat-killed fungal discs, 10h contact time at pH: 5.0 and room temperature. Additionally, each disc was able to actively use for at least 6 more cycles in biosorption experiments. The specific removal rate was calculated as 0.114 day−1, the rate constant and half-life period were also 1.609 day-1, t1/2 = 0.431, respectively. The kinetic study was described by the pseudo-second order model and the equilibrium modeling was found to be well fitted with Langmuir isotherm. The biosorbent(s) were characterized by Focused Ion Beam Scanning Electron Microscopy (FIB-SEM). Over 80% removal of long-chain n-alkanes by the heat-killed fungal discs was confirmed by GC-MS analysis. Since there has been no similar study investigating the sorption of petroleum with heat-killed Aspergillus ochraceous discs, this novel bio-based sorbent with its low cost, environmentally friendly and easy-to-apply properties can be used in advanced biosorption studies.

Keywords

Petroleum, Biosorption, Heat-killed Aspergillus ochraceus, Biosorption Isotherm and Kinetics

Supporting Institution

Hacettepe University

Project Number

FHD-2019-17719

Thanks

This work is funded by Hacettepe University Scientific Research Projects Coordination Unit (Project number: FHD-2019-17719).

References

• 1. Pi Y, Chen B, Bao M, Zhang B. Microbial degradation of four dispersed crude oils by Rhodococcus sp. evaluated using carbon stable isotope analysis. J Chem Technol Biotechnol 2019; 94 (6): 1800–7.
• 2. Chen ZK, An C, Boufadel M, Owens E, Chen Z, Lee K, Cao Y, Cai M. Use of surface-washing agents for the treatment of oiled shorelines: Research advancements, technical applications and future challenges. Chem Eng J 2020; 391: 123565.
• 3. Liu B, Chen B, Zhang B, Song X, Zen G, Lee K. Photocatalytic ozonation of offshore produced water by TiO2 nanotube arrays coupled with UV-LED Irradiation. J Hazard Mater 2020; 40: 123456.
• 4. Tian Y, Li J, Whitcombe TW, McGill WB, Thring R. Application of oily sludge-derived char for lead and cadmium removal from aqueous solution. Chem Eng J 2020; 384: 123386.
• 5. Zhu Z, Zhang B, Cai Q, Ling J, Lee K, Chen B. Fish waste based lipopeptide production and the potential application as a biodispersant for oil spill control. Front Bioeng Biotechnol 2020; 8: 734.
• 6. Kapahi M, Sachdeva S. Bioremediation options for heavy metal pollution. J Heal Pollut 2019; 9 (24): 191203.
• 7. Nazir H, Salman M, Athar M, Farooq U, Wahab A, Akram M. Citric acid functionalized Bougainvillea spectabilis: a novel, sustainable, and cost-effective biosorbent for removal of heavy metal (Pb2+) from wastewater. Water Air Soil Pollut 2019; 230: 1-16.
• 8. Akram M, Salmana M, Farooq U, Saleem U, Tahir S, Nazira H, Arsalan HM. Phthalate-functionalized Sorghum bicolor L.; an effective biosorbent for the removal of alizarin red S and bromophenol blue dyes from simulated wastewater. Desalin Water Treat 2020; 190: 383-92.
• 9. Shen J, Huang G, An C, Zha S, Rosendahl S. Immobilization of tetrabromobisphenol a by pinecone-derived biochars at solidliquid interface: synchrotron-assisted analysis and role of inorganic fertilizer ions. Chem Eng J 2017; 321: 346–57.
• 10. Cai L, Zhang Y, Zhou Y, Zhang X, Ji L, Song W, Zhang H, Liu J. Effective adsorption of diesel oil by crab-shell-derived biochar nanomaterials. Materials 2019;12 (2): 236.
• 11. Nazir H, Salman M, Athara M, Farooq U, Akrama M, Saleem N. A novel biosorbent B. spectabilisis stalks leaves for removal of Cd (II) and Cu (II) from wastewater. Desalin Water Treat 2019; 148: 222-28.
• 12. Hess BJ, Kolar P, Classen JJ, Knappe D, Cheng JJ. Effects of co occurring species present in swine lagoons on adsorption of copper on eggshell. Int J Environ Res 2019; 13: 613–22.
• 13. Joseph EE, Azorji, JN, Iheanacho JC, Nwachukwu CU, Iheagwam KS, Okoli CJ. Adsorption of petroleum hydrocarbons from crude oil polluted soil using agro-waste. J Environ Treat Tech 2022; 10 (4): 284-89.
• 14. Saleem U, Akram M, Salman M, Farooq U, Dar A, Tahir S. Biosorption activity of chemically modified Trifolium alexandrinum for the detoxification of Cr (III) contaminated water. Desalin water Treat 2022; 246: 212–25.
• 15. Sasidharan R, Kumar A. Magnetic adsorbent developed with alkali thermal pretreated biogas slurry solids for the removal of heavy metals: optimization, kinetic, and equilibrium study. Environ Sci Pollut Res 2022; 29 (20): 30217–32.
• 16. Olivella MÀ, Jové P, Bianchi A, Bazzicalupi C, Cano L. An integrated approach to understanding the sorption mechanism of phenanthrene by cork. Chemosphere 2013; 90 (6): 1939–44.
• 17. Christensen KM, Rorrer GL. Equilibrium partitioning behavior of naphthalene and phenanthrene with axenic microplantlets of the temperate green seaweed Acrosiphonia coalita. Chemosphere 2009; 76 (8): 1135–42.
• 18. Hussein M, Amer AA, Zahran HF, Ali SM, Elgohary M, Nasr M. Agricultural waste as a biosorbent for oil spills. Int J Dev 2013; 2 (1): 127-35.
• 19. El Gheriany IA, El Saqa FA, Amer AAER, Hussein M. Oil spill sorption capacity of raw and thermally modified orange peel waste. Alex Eng J 2020; 59(2): 925-32.
• 20. Al Zubaidi I, Al Zubaidi M, Tajik M, Al Zubaidi M, Al Mutair M, Sheikh M, Chen Y, Al-Yasiri M, Alsudays A. Pomegranate peels powder for the remediation of oil polluted water from waste lubricating oil. Proceedings of the 5th International Conference of Fluid Flow, Heat and Mass Transfer, Niagara Falls, Canada, 2018.
• 21. Wu J, Yu HQ. Biosorption of 2,4-dichlorophenol from aqueous solution by Phanerochaete chrysosporium biomass: isotherms, kinetics, and thermodynamics. J Hazard Mater 2006; 137: 498–508.
• 22. Chung M, Tsui MT, Cheung K, Tam NF, Wong MH. Removal of aqueous phenanthrene by brown seaweed Sargassum hemiphyllum: Sorptionkinetic and equilibrium studies. Sep Purif Technol 2007; 54 (3): 355–62.
• 23. Crognale S, Annibale AD, Pesciaroli L, Stazi SR. Fungal community structure and as-resistant fungi in a decommissioned gold mine site. Front Microbiol 2017; 8: 2202.
• 24. Al-Hawash AB, Zhang X, Ma F. Removal and biodegradation of different petroleum hydrocarbons using the filamentous fungus Aspergillus sp. RFC-1. Microbiol Open 2019; 8 (1): e00619.
• 25. Dusengemungu L, Kasali G, Gwanama C, Ouma KO. Recent Advances in Biosorption of Copper and Cobalt by Filamentous Fungi. Front Microbiol 2020; 11: 582016.
• 26. Shah SS, Palmieri MC, Sponchiado SRP, Bevilaqua D. Enhanced bio-recovery of aluminum from low-grade bauxite using adapted fungal strains. Braz J Microbiol 2020; 51: 1909-18.
• 27. Oladipo OG, Awotoye OO, Olayinka A, Bezuidenhout CC, Maboeta MS. Heavy metal tolerance traits of filamentous fungi isolated from gold and gemstone mining sites. Braz J Microbiol 2018; 49: 29–37.
• 28. Chen L, Li E, Wu W, Wang G, Zhang J, Guo X, Xing F. The secondary metabolites and biosynthetic diversity from Aspergillus ochraceus. Front in Chem 2022; 10: 938626.
• 29. Hareeri RH, Aldurdunji MM, Abdallah HM, Alqarni AA, Mohamed SGA, Mohamed GA, Ibrahim SRM. Aspergillus ochraceus: metabolites, bioactivities, biosynthesis, and biotechnological potential. Molecules 2022; 27 (19): 6759.
• 30. Vahabisani A, An C. Use of biomass derived adsorbents for the removal of petroleum pollutants from water: a mini review. Environ Syst Res 2021; 10 (1): 1-10.
• 31. Rao J, Viraraghavan T. Biosorption of phenol from an aqueous solution by Aspergillus niger biomass. Bioresour Technol 2002; 85 (2): 165–71.
• 32. Kumar R, Bishnoi NR, Bishnoi K. Biosorption of chromium (VI) from aqueous solution and electroplating wastewater using fungal biomass. Chem Eng J 2008; 135 (3): 202–8.
• 33. Lu N, Hua T, Zhai Y, Qin H, Aliyeva J, Zhang H. Fungal cell with artificial metal container for heavy metals biosorption: Equilibrium, kinetics study and mechanisms analysis. Environ Res 2020; 182: 109061.
• 34. Raghukumar C, Shailaja M, Parameswaran P, Singh S. Removal of polycyclic aromatic hydrocarbons from aqueous media by the marine fungus NIOCC 312: Involvement of lignin-degrading enzymes and exopolysaccharides. Indian J Mar Sci 2006; 35 (4): 373-79.
• 35. Bilen Ozyurek S, Avcioglu NH, Seyis Bilkay I. Mycoremediation potential of Aspergillus ochraceus NRRL 3174. Arch Microbiol 2021; 203: 5937-50.
• 36. Mohammadi M, Sedighi M, Hemati M. Removal of petroleum asphaltenes by improved activity of NiO nanaoparticles supported on green AlPO-5 zeolite: process optimization and adsorption isotherm. Petroleum 2020; 6 :182-88.
• 37. Benguenab A, Chibani A. Biodegradation of petroleum hydrocarbons by filamentous fungi (Aspergillus ustus and Purpureocillium lilacinum) isolated from used engine oil contaminated soil. Acta Ecologica Sinica 2021; 41 (5): 416-23.
• 38. Bilen Ozyurek S, Seyis Bilkay I. Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum contaminated waste mud pit. SN Appl Sci 2020; 2: 1-12.
• 39. Kachieng’a L, Momba MNB. Kinetics of petroleum oil biodegradation by consortium of three protozoan isolates (Aspidisca sp., Trachelophyllum sp. and Peranema sp.). Biotechnol Rep 2017; 15: 125–31.
• 40. Cheng Z, Feng K, Su Y, Ye J, Chen D, Zhang S, Zhang X, Dionysiou DD. Novel biosorbents synthesized from fungal and bacterial biomass and their applications in the adsorption of volatile organic compounds. Bioresour Technol 2020; 300: 122705.
• 41. Kim N, Seo JH, Yun YS, Park D. New insight into continuous recirculation process for treating arsenate using bacterial biosorbent. Bioresour Technol 2020; 316: 123961.
• 42. Saratale GD, Kalme SD, Govindwar SP. Decolorisation of textile dyes by Aspergillus ochraceus (NCIM-1146). Ind J Biotechnol 2006; 5: 407-10.
• 43. Legorreta-Castañeda AJ, Lucho-Constantino CA, Beltrán- Hernández RI, Coronel-Olivares C, Vázquez-Rodríguez GA. Biosorption of water pollutants by fungal pellets. Water 2020; 12 (4): 1155.
• 44. Kumar Mishra P, Mukherji S. Biosorption of diesel and lubricating oil on algal biomass. 3 Biotech 2012; 2: 301–10.
• 45. Simonescu CM, Ferdes M. Fungal biomass for Cu (II) uptake from aqueous systems. Pol J Environ Stud 2012; 21 (6): 1831-39.
• 46. Latha JNL, Babu PN, Rakesh P, Kumar KA, Anupama M, Susheela L. Fungal cell walls as protective barriers for toxic metals. Adv Med Biol 2012; 53: 181-98
• 47. Boleydei H, Mirghaffari N, Farhadian O. Comparative study on adsorption of crude oil and spent engine oil from seawater and freshwater using algal biomass. Environ Sci Pollut Res 2018; 25: 21024-35.
• 48. Jalali R, Ghafourian H, Asef Y, Davarpanah S, Sepehr S. Removal and recovery of lead using nonliving biomass of marine algae. J Hazard Mater 2002; 92 (3): 253–62.
• 49. Aracagok YD. Biosorption of lead by a soil isolate Aspergillus neoalliaceus. Arch Microbiology 2022; 204 (9): 547.
• 50. Devi MG, Al-Hashmi ZS, Sekhar GC. Treatment of vegetable oil mill effluent using crab shell chitosan as adsorbent. Int J Environ Sci Technol 2012; 9: 713–18.
• 51. Mwandira W, Nakashima K, Kawasaki S, Arabelo A, Banda K, Nyambe I, Chirwa M, Ito M, Sato T, Igarashi T, Nakata H, Nakayama S, Ishizuka M. Biosorption of Pb (II) and Zn (II) from aqueous solution by Oceanobacillus profundus isolated from an abandoned mine. Sci Rep 2020; 10 (1): 21189.
• 52. Tsekova T, Todorova D, Dencheva V, Ganeva S. Biosorption of copper (II) and cadmium (II) from aqueous solutions by free and immobilized biomass of Aspergillus niger. Bioresour Technol 2010; 101 (6): 1727-31.
• 53. Tsekova T, Todorova D, Ganeva S. Removal of heavy metals from industrial wastewater by free and immobilized cells of Aspergillus niger. Int Biodeterior Biodegrad 2010; 64 (6): 447-51.
• 54. Lim MW, Von Lau E, Poh PE A. A comprehensive guide of remediation technologies for oil contaminated soil—present works and future directions. Mar Pollut Bull 2016; 109 (1): 14–45.
• 55. Zhou JL, Kiff RJ. The uptake of copper from aqueous solution by immobilized fungal biomass. J Chem Technol Biot 1991; 52 (3): 317–30.
• 56. Hasan IF. Role of filamentous fungi to remove petroleum hydrocarbons from the environment, In Microbial Action on Hydrocarbons, Springer, Singapore; 2019.
• 57. Xu N, Bao M, Sun P, Li Y. Study on bioadsorption and biodegradation of petroleum hydrocarbons by a microbial consortium. Bioresour Technol 2013; 149: 22–30.
• 58. Barnes NM, Khodse VB, Lotlikar NP, Meena RM, Damare SR. Bioremediation potential of hydrocarbon-utilizing fungi from select marine niches of India. 3 Biotech 2018; 8: 1-10.
• 59. Sokker HH, El-Sawy NM, Hassan MA, El-Anadouli BE. Adsorption of crude oil from aqueous solution by hydrogel of chitosan-based polyacrylamide prepared by radiation induced graft polymerization. J Hazard Mater 2011; 190 (1-3): 359-65.
• 60. Singh S, Parveen N, Gupta H. Adsorptive decontamination of rhodamine-B from water using banana peel powder: a biosorbent. Environ Technol Innov 2018; 12: 189-95.
• 61. Peng D, Ouyang F, Liang X, Guo X, Dang Z, Zheng L. Sorption of crude oil by enzyme-modified corn stalk vs. chemically treated corn stalk. J Mol Liq 2018; 255: 324-32.
• 62. Mottaghi H, Mohammadi Z, Abbasi M, Tahouni N, Panjeshahi MH. Experimental investigation of crude oil removal from water using polymer adsorbent. Journal of Water Process Engineering 2021; 40: 101959.
• 63. Mallampati R, Xuanjun L, Adin A, Valiyaveettil S. Fruit peels as efficient renewable adsorbents for removal of dissolved heavy metals and dyes from water. ACS Sustain Chem Eng 2015; 3 (6): 1117-1124.
• 64. Zhang B, Yu S, Zhu Y, Shen Y, Gao X, Shi W, Tay JH. Adsorption mechanisms of crude oil onto polytetrafluoroethylene membrane: kinetics and isotherm, and strategies for adsorption fouling control. Sep Purifi Technol 2020; 235: 116212.
• 65. Yu X, He Y. Optimal ranges of variables for an effective adsorption of lead (II) by the agricultural waste pomelo (Citrus grandis) peels using Doehlert designs. Sci Rep 2018; 8 (1): 729.
• 66. Liang J, Cheng T, Huang Y, Liu J. Petroleum degradation by Pseudomonas sp. ZS1 is impeded in the presence of antagonist Alcaligenes sp. CT10. AMB Expr 2018; 8 (1): 1-13.
• 67. Al-Dhabi NA, Esmail GA, Arasu MV. Enhanced production of biosurfactant from Bacillus subtilis strain Al-dhabi-130 under solid-state fermentation using date molasses from Saudi Srabia for bioremediation of crude-oil-contaminated soils. Int Env Res Pub He 2020; 17 (22): 8446.
• 68. Luo X, Chen L, Zhao S, Lei Z, Xia M, Zhang C. Study of the characteristics of two immobilized microbial materials in degradation and evolution of petroleum hydrocarbon. ACS Omega 2020; 5 (31): 19402−8.
• 69. Guo H, Tang S, Xie S, Wang P, Huang C, Geng X, Jia X, Huo H, Li X, Zhang J, Zhang Z, Fang J. The oil removal and the characteristics of changes in the composition of bacteria based on the oily sludge bio electrochemical system. Sci Rep 2020; 10 (1): 15474.
• 70. Liu S, Yuan W, Yang X, Li C, Ji Y, Feng X, Li M, Xie Y. Microbial degradation of petroleum characteristic pollutants in hypersaline environment, emphasizing n-hexadecane and 2,4 di-tertbutylphenol. Environ Monit Assess 2023; 195 (6): 771.