Review of Chromatography Methods for Purfication of Paraoxonase Enzyme

Year 2018, , 271 - 274, 31.12.2018

Basak Gokce

https://doi.org/10.17350/HJSE19030000102

Abstract

Human serum paraoxonase1 hPON1 is a calcium dependent enzyme, which has an important physiological role in organism with detoxification, antioxidant and also antiatherogenic properties. PON1 is mostly synthesized from the liver than secreted in serum with located on HDL. In order to better understand the molecular mechanism of paraoxonase 1, researchers have been increasingly interested in the development of various methods for obtaining the enzyme in pure form over the last decades. In this paper, several different purification and characterization methods of multi functional enzyme paraoxonase 1 EC 3.3.8.1 was reviewed.

Keywords

Paraoxonase1, Chromatography Methods, Enzyme Purification and Characterization

References

• Van Himbergen TM, Van Tits LJH, Roest M, Stalenhoef AFH. The story of PON1: how an organophosphate hydrolyzing enzyme is becoming a player in cardiovascular medicine. Neth J Med 2006;64(2):34–8.
• Hegele RA. Paraoxonase genes and disease. Ann Med. 1999;31: 217–224
• Protein Purifcation. Principles, high resolution methods and applications. Third edition. Edited by J-C Jansson, 2011. Publ. John Wiley & Sons. ISBN 978-0-471-74661-4.
• Renault F., Chabriere E., Andrieu JP , Dublet B., Masson P., Rochu D. Tandem purification of two HDL-associated partner proteins in human plasma, paraoxonase (PON1) and phosphate binding protein (HPBP) using hydroxyapatite chromatography Journal of Chromatography B, 836 (2006) 15–21
• C.E. Furlong, R.J. Richter, C. Chapline, J.W. Crabb, Biochemistry 30 (1991) 10133.
• K N Gan, A Smolen, H W Eckerson and B N La Du Purification of human serum paraoxonase/arylesterase. Evidence for one esterase catalyzing both activities. Drug Metabolism and Disposition January 1991, 19 (1) 100-106
• Rodrigo L, Gil F, Hernandez AF, et al. Purification and characterization of paraoxon hydrolase from rat liver. Biochem J 1997;321: 595–601 Cebeci BK, Alım Z, Beydemir Ş. In vitro effects of pesticide exposure on the activity of the paraoxonase-1 enzyme from sheep liver microsomes Turk Journal of Chem (2014) 38: 512–520
• Beydemir S, Ekinci D, Ates O. (2009). In vitro effects of dexamethasone on human serum Paraoxonase-I (PON1) Activity. Hacettepe J Biol Chem, 37:197–205 Sinan S, Kockar F, Arslan O. Novel purification strategy for humanPON1 and inhibition of the activity by cephalosporin andaminoglikozide derived antibiotics. Biochimie 2006;88:565–74
• Sayın D, Cakır DT, Gencer N, Arslan O. Effects of some metals onparaoxonase activity from shark Scyliorhinus canicula. J EnzymeInhib Med Chem 2012;27:595–8. Erzengin M, Demir D, Arslan M, Sinan S. Purification and Characterization of Paraoxonase 1 (PON1) from Swiss Black, Holstein, and Montofon Bovines Appl Biochem Biotechnol (2014) 173:1597–1606
• Erol K, Gencer N, Arslan M, Arslan O. Purification, characterization, and investigation of in vitro inhibition by metals of paraoxonase from different sheep breeds. Artif Cells Nanomed Biotechnol 2013;41:125–30.
• Ekinci D, Senturk M, Beydemir S, et al. (2010). An alternativepurification of human serum paraoxonase 1 and interactions withsulfonamides. Chem Biol Drug Des, 76:552–8
• Ekinci D., Beydemir Ş. Purification of PON1 from “Human Serum and Assessment of Enzyme Kinetics Against Metal Toxicity” Biol Trace Elem Res (2010) 135:112–120
• Colak U, Gencer N. “Immobilization of paraoxonase onto chitosanan its characterization”. Artif Cells Blood Substit Immobil Biotechnol 2012;40:290–5
• Demir D, Gencer N, Arslan O. “An alternative purification method for human serum paraoxonase 1 and its interactions with anabolic compounds”. J Enzyme Inhib Med Chem. 2016;31(2):247-52.
• Gencer N, Yavuz E. “An alternative purification method for human serum paraoxonase 1 and its interaction with methidathion” Arch Physiol Biochem, 2017; 123(3): 159– 164