Enhanced Enzyme Activity with Ferritin Nanocages

Year 2018, , 115 - 118, 29.06.2018

Eylem Turan

https://doi.org/10.17350/HJSE19030000081

Abstract

I n this study, a novel enzyme system based on ferritin nanocages was designed. α-Amylase was covalently crosslinked on surface of ferritin nanocages by EDC/NHS. The activity of immobilized enzyme was monitored by using UV-vis spectrophotometer. The optimum temperature of immobilized enzyme shifted from 50 oC to 70 oC due to the ferritin nanocages. In addition, The Km and Vmax values of immobilized enzymes were 5.19 mg mL-1 and 3.3x10-5 U mg-1, respectively. This novel enzyme system displayed higher catalytic activity and enhanced stability

Keywords

Ferritin nanocages, α-amylase, Enzyme immobilization, EDC/NHS, Covalently crosslinked

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