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Year 2017, , 45 - 49, 30.06.2017
https://doi.org/10.17350/HJSE19030000047

Abstract

References

  • 1. Wu S, Liu X, Yeung KW, Liu C, Yang X. Biomimetic porous scaffolds forbone tissue engineering, Mater. Sci. Eng. R. Rep. 80 (2014) 1–36.
  • 2. Levengood SKL, Zhang M. Chitosan-based scaffolds for bone tissue engineering, J. Mater. Chem. B 2, (2014) 3161– 3184.
  • 3. Afshar HA, Ghaee A. Preparation of aminated chitosan/ alginatescaffold containing halloysite nanotubes with improved cell attachment. Carbohydrate Polymers 151 (2016) 1120–1131.
  • 4. Kumar MNRL. A review of chitin and chitosan applications. Reactive and Functional Polymers 46, (2000) 1–27.
  • 5. Hu Q, Li B, Wang M, Shen J. Preparation and characterization of biodegradable chitosan/hydroxyapatite nanocomposite rods via in situ hybridization: a potential material as internal fixation of bone fracture. Biomaterials 25 (2004) 779–85.
  • 6. Kumar RL, Narayan AK, Dhivya S, Chawla A, Saravanan S, and Selvamurugan N. A review of chitosan and its derivatives in bone tissue engineering. Carbohydrate Polymers 151 (2016) 172–188.
  • 7. Puppi D, Chiellini F, Piras AM, Chiellini E. Polymeric materials forbone and cartilage repair. Progress in Polymer Science 35 (2010) 403–440.
  • 8. Kai D, Prabhakaran MP, Stahl B, Eblenkamp M, Wintermantel E, Ramakrishna S. Mechanical properties and in vitro behavior of nanofiber-hydrogel composites for tissue engineering applications. Nanotechnology 23 (2012) 1–10.
  • 9. Rezwan K, Chen QZ, Blaker JJ, Boccaccini AR. Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials 27 (2006) 3413–3431.
  • 10. Choy JH, Choi SJ, Oh JM, Park T. Clay minerals and layered double hydroxides for novel biological applications Applied Clay Science 36 (2007) 122–132.
  • 11. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65 (1983) 55-63.
  • 12. Loh QL and Choong C. Three-dimensional scaffolds for tissue engineering applications: Role of porosity and pore size. Tissue Eng Part B Rev. 19(6) (2013) 485–502.
  • 13. Khan F, Tanaka M, Rafi Ahmad S. Fabrication of polymeric biomaterials: a strategy for tissue engineering and medical devices. J. Mater. Chem. B 3 (2015) 8224-8249.
  • 14. Christenson RH. Biochemical markers of bone metabolism: an overview. Clin Biochem. 30 (1997) 573–593.

Evaluation of Chitosan /Organoclay Composite as Bone Tissue Engineering Scaffold

Year 2017, , 45 - 49, 30.06.2017
https://doi.org/10.17350/HJSE19030000047

Abstract

I n this study, a scaffold composed of chitosan C and organoclay OC was prepared by utilizing freeze-drying method. The composition of resulting composite scaffold was evaluated using FTIR and SEM. The in vivo cytocompatibility of the composite was evaluated in vivo, by seeding MC3T3-E1 cells on composite scaffolds. In-vitro cell viability and proliferation were investigated using the MTT assay, while cell-scaffold constructs were evaluated using scanning electron microscopy. Preliminary in vivo experiments were also performed to evaluate tissue compatibility and ectopic boneforming potential of C/OC composite scaffolds with MC3T3-E1 cells. The findings suggest that C/OC construct supported the proliferation of MC3T3-E1 cells, presented histobiocompatibility and promoted formation of calcified matrix. Based on the results, we conclude that the characterized C/OC composite system may have potential for use in bone tissue engineering applications

References

  • 1. Wu S, Liu X, Yeung KW, Liu C, Yang X. Biomimetic porous scaffolds forbone tissue engineering, Mater. Sci. Eng. R. Rep. 80 (2014) 1–36.
  • 2. Levengood SKL, Zhang M. Chitosan-based scaffolds for bone tissue engineering, J. Mater. Chem. B 2, (2014) 3161– 3184.
  • 3. Afshar HA, Ghaee A. Preparation of aminated chitosan/ alginatescaffold containing halloysite nanotubes with improved cell attachment. Carbohydrate Polymers 151 (2016) 1120–1131.
  • 4. Kumar MNRL. A review of chitin and chitosan applications. Reactive and Functional Polymers 46, (2000) 1–27.
  • 5. Hu Q, Li B, Wang M, Shen J. Preparation and characterization of biodegradable chitosan/hydroxyapatite nanocomposite rods via in situ hybridization: a potential material as internal fixation of bone fracture. Biomaterials 25 (2004) 779–85.
  • 6. Kumar RL, Narayan AK, Dhivya S, Chawla A, Saravanan S, and Selvamurugan N. A review of chitosan and its derivatives in bone tissue engineering. Carbohydrate Polymers 151 (2016) 172–188.
  • 7. Puppi D, Chiellini F, Piras AM, Chiellini E. Polymeric materials forbone and cartilage repair. Progress in Polymer Science 35 (2010) 403–440.
  • 8. Kai D, Prabhakaran MP, Stahl B, Eblenkamp M, Wintermantel E, Ramakrishna S. Mechanical properties and in vitro behavior of nanofiber-hydrogel composites for tissue engineering applications. Nanotechnology 23 (2012) 1–10.
  • 9. Rezwan K, Chen QZ, Blaker JJ, Boccaccini AR. Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials 27 (2006) 3413–3431.
  • 10. Choy JH, Choi SJ, Oh JM, Park T. Clay minerals and layered double hydroxides for novel biological applications Applied Clay Science 36 (2007) 122–132.
  • 11. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65 (1983) 55-63.
  • 12. Loh QL and Choong C. Three-dimensional scaffolds for tissue engineering applications: Role of porosity and pore size. Tissue Eng Part B Rev. 19(6) (2013) 485–502.
  • 13. Khan F, Tanaka M, Rafi Ahmad S. Fabrication of polymeric biomaterials: a strategy for tissue engineering and medical devices. J. Mater. Chem. B 3 (2015) 8224-8249.
  • 14. Christenson RH. Biochemical markers of bone metabolism: an overview. Clin Biochem. 30 (1997) 573–593.
There are 14 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Aysel Koc Demir This is me

Publication Date June 30, 2017
Published in Issue Year 2017

Cite

Vancouver Demir AK. Evaluation of Chitosan /Organoclay Composite as Bone Tissue Engineering Scaffold. Hittite J Sci Eng. 2017;4(1):45-9.

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