BibTex RIS Cite
Year 2014, , 37 - 41, 31.12.2014
https://doi.org/10.17350/HJSE19030000006

Abstract

References

  • Oh J, Jo H, Cho AR, Kim SJ, Han J. Antioxidant and antimicrobial activities of various leafy herbal teas. Food Control 31 (2013) 403-409.
  • Exarchou V, Nenadis N, Tsimidou M. Antioxidant activities and phenolics composition of extracts from Greek oregano, Greek sage and Summer savory. Journal of Agricultural and Food Chemistry 50 (19) (2002) 5294-5299.
  • Salvamani S, Gunasekaran B, Shaharuddin NA, Ahmad SA, Shukor MY. Antiartherosclerotic effects of plant flavonoids. BioMed Research International 2014 (2014) 1-11.
  • Sohn HY, Son KH, Kwon CS, Kwon GS, Kang SS. Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. 18. Phytomedicine 11 (2004) 666–672.
  • Muthumani GD, Vijay Anan A, Manikandan A. Antioxidant, antihelmintic and antimicrobial activity of Euphorbia thymifolia Linn whole plant. International Journal of Current Microbiology and Applied Sciences 2 (2013) 66.
  • Kahkonen MP, Hopia AI, Vuorela HJ, Rauh JP, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry 47 (1999) 3954 3962.
  • Wang W, Zu Y, Fu Y, Efferth T. In vitro antioxidant and antimicrobial activity of extracts from Morus alba L. leaves, stems and fruits. The American Journal of Chinese Medicine 40(2) (2012) 349-356.
  • Aditya Rao SJ, Ramesh CK, Riaz M, Prabhakar BT. Anthelmintic and antimicrobial activities in some species of mulberry. International Journal of Pharmacy and Pharmaceutical Sciences 4 (2012) 335-338. Shanmugasundaram P. GC-MS analysis of phytochemical constituents in ethanolic bark extract of Ficus religiosa LINN. Int. J. Pharm. Sci. 6 (2014) 457-460.
  • Gobalakrishnan R, Manikandan P, Bhuvaneswari R. Antimicrobial potential and bioactive constituents from aerial parts of Vitis setosa Wall. Journal of Medicinal Plants Research 8(11) (2014) 454-460.

Antioxidant and antimicrobial activities with GC/MS analysis of the Morus alba L. Leaves

Year 2014, , 37 - 41, 31.12.2014
https://doi.org/10.17350/HJSE19030000006

Abstract

Morus alba L. Moraceae leaves are used effectively to treat fever, protect liver from damage, strengthen the joints, facilitate discharge of urine in Turkey folk medicine. In this study we aimed to determine in vitro antioxidant and antimicrobial activity with GC/MS analysis of the Morus alba L. leaves. Dried plant leaves samples are milled and extracted with distilled water and ethanol in Soxhlet machine. After extraction, extract samples were concentrated in rotary evaporator machine. Total antioxidant status values were determined as mmol Trolox Equivalent/L spectrophotometrically by using Erel’s method. Sterile extracts were used to avoid contamination in antimicrobial activity test. As test microorganisms Escherichia coli ATCC 25922 , Staphylococcus aureus ATCC 25923 , Enterococcus faecalis ATCC 29212 , Pseudomonas aeroginosa ATCC 27853 , Candida albicans ATCC 90028 were used. Antimicrobial activity was determined with disc diffusion method. We used ethanol extracts of samples in GC/MS analysis. Ethanol and distilled water extracts showed antioxidant activity in different rate. Besides, ethanol extracts have an antimicrobial activity but water extracts have not antimicrobial activity on our test microorganisms. We determined four compounds 9,12,15-octadecatrienoic acid, ethyl ester, linolenic acid ethyl ester, gibberellic acid with GC/MS analysis in ethanol extracts

References

  • Oh J, Jo H, Cho AR, Kim SJ, Han J. Antioxidant and antimicrobial activities of various leafy herbal teas. Food Control 31 (2013) 403-409.
  • Exarchou V, Nenadis N, Tsimidou M. Antioxidant activities and phenolics composition of extracts from Greek oregano, Greek sage and Summer savory. Journal of Agricultural and Food Chemistry 50 (19) (2002) 5294-5299.
  • Salvamani S, Gunasekaran B, Shaharuddin NA, Ahmad SA, Shukor MY. Antiartherosclerotic effects of plant flavonoids. BioMed Research International 2014 (2014) 1-11.
  • Sohn HY, Son KH, Kwon CS, Kwon GS, Kang SS. Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. 18. Phytomedicine 11 (2004) 666–672.
  • Muthumani GD, Vijay Anan A, Manikandan A. Antioxidant, antihelmintic and antimicrobial activity of Euphorbia thymifolia Linn whole plant. International Journal of Current Microbiology and Applied Sciences 2 (2013) 66.
  • Kahkonen MP, Hopia AI, Vuorela HJ, Rauh JP, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry 47 (1999) 3954 3962.
  • Wang W, Zu Y, Fu Y, Efferth T. In vitro antioxidant and antimicrobial activity of extracts from Morus alba L. leaves, stems and fruits. The American Journal of Chinese Medicine 40(2) (2012) 349-356.
  • Aditya Rao SJ, Ramesh CK, Riaz M, Prabhakar BT. Anthelmintic and antimicrobial activities in some species of mulberry. International Journal of Pharmacy and Pharmaceutical Sciences 4 (2012) 335-338. Shanmugasundaram P. GC-MS analysis of phytochemical constituents in ethanolic bark extract of Ficus religiosa LINN. Int. J. Pharm. Sci. 6 (2014) 457-460.
  • Gobalakrishnan R, Manikandan P, Bhuvaneswari R. Antimicrobial potential and bioactive constituents from aerial parts of Vitis setosa Wall. Journal of Medicinal Plants Research 8(11) (2014) 454-460.
There are 9 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Asiye Asli Emniyet This is me

Emre Avci This is me

Burcin Ozcelik This is me

Gulcin Alp Avci This is me

Dursun Ali Kose This is me

Publication Date December 31, 2014
Published in Issue Year 2014

Cite

Vancouver Emniyet AA, Avci E, Ozcelik B, Avci GA, Kose DA. Antioxidant and antimicrobial activities with GC/MS analysis of the Morus alba L. Leaves. Hittite J Sci Eng. 2014;1(1):37-41.

Hittite Journal of Science and Engineering Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı (CC BY NC) ile lisanslanmıştır.