Separation and purification of lipase using Cu nanoparticle embedded poly HEMA-MATrp cryogels

Abstract

Quality and efficiency of techniques to be used for separation and purification lipase enzymes are commercially important enzyme. Among such techniques, adsorption methods are highly preferred. Cryogels have been quite extensively used as the adsorbents due to their macropores and interconnected flow channels. In this study, adsorption of lipase enzyme onto copper nanoparticles embedded poly 2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan , poly HEMA-MATrp cryogels was studies for conditions with varying pH, interaction time, lipase enzyme initial concentration, temperature and ionic strength. Maximum lipase enzyme adsorption capacity of cryogels was determined as 183.6 mg/g. Fourier transform infrared spectrometer FTIR and scanning electron microscopy SEM were used for characterization of cryogels. At the end of the adsorption process, in order to be sure that the purity of lipase enzyme desorbed from cryogels, SDS-PAGE analyses were performed and molecular weight of the lipase enzyme was determined as 58 kDa. Adsorption characteristic of cryogels were determined according to the results of Langmuir and Freundlich adsorption isotherm models. As a result of calculation run for adsorption isotherm models, Langmuir isotherm model was determined to be more appropriate.

Keywords

• Lipase,Cupper,Cryogel,Adsorption.

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