Some Serum Oxidative Parameters in Normoglycemic Rats: Vascular Endothelial Growth Factor VEGF Application

Year 2020, Volume: 7 Issue: 1, 55 - 59, 26.03.2020

Kaan Kaltalioglu , Sule Coskun Cevher

https://doi.org/10.17350/HJSE19030000173

Abstract

V female normoglycemic Wistar rats were used 200–250 g . Dorsolateral incisional wounds length: 4 cm were made on the two sides of the medulla spinalis in rats. They were di- vided into 3 groups: untreated group n=12 , chitosan group n=12 and chitosan+VEGF group n=12 . The rats were sacrificed on the 3rd and 7th days of post wounding. NOx, TBARs and RSH levels were determined spectrophotometrically in serum. Results were compared by one-way ANOVA p

Keywords

VEGF application, Chitosan, Wound healing, Oxidative stress, Antioxidant

References

• 1. Harding KG, Morris HL, Patel GK. Science, medicine and the future: Healing chronic wounds. BMJ 324 (2002) 160-163.
• 2. Fahey TJ, Sadaty A, Jones WG, Barbe A, Smoller B, Shires GT. Diabetes impairs the late inflammatory response to wound healing. J Surg Res 50 (1991) 308-313.
• 3. Lerman OZ, Galiano RD, Armour M, Levine JD, Gurtner, GC. Cellular dysfunction in the diabetic fibroblast. Am J Pathol 162 (2003) 303-312.
• 4. Komesu MC, Tanga MB, Buttros KR, Nakao C. Effects of acute diabetes on rat cutaneous wound healing. Pathophysiology 11 (2004) 63-67.
• 5. Lobmann R, Ambrosch A, Schultz G, Waldmann K, Schiweck S, Lehnert H. Expression of matrix-metalloproteinases and their inhibitors in the wound of diabetic and non-diabetic patients. Diabetologia 45 (2002) 1011-1016.
• 6. Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors. Nat Med 9 (2003) 669–676.
• 7. Poltorak Z, Cohen T, Neufeld G. The VEGF splice variants: properties, receptors, and usage for the treatment of ischemic diseases. Herz 25 (2000) 126–129.
• 8. Tammela T, Enholm B, Alitalo K, Paavonen K. The biology of vascular endothelial growth factors. Cardiovasc Res 65 (2005) 550–563.
• 9. Ferrara N, Davis-Smith T. The biology of vascular endothelial growth factor. Endocr Rev 18 (1997) 4–25.
• 10. Sun T, Yao Q, Zhou D, Mao F. Antioxidant activity of N-carboxymethyl chitosan oligosaccharides. Bioorg Med Chem Lett 18 (2008) 5774-5776.
• 11. Sen CK, Khanna S, Babior BM, Hunt TK, Ellison EC, Roy S. Oxidant-induced vascular endothelial growth factor expression in human keratinocytes and cutaneous wound healing. J Biol Chem 277 (2002) 33284-33290.
• 12. Aktunc E, Ozacmak VH, Ozacmak HS, Barut F, Buyukates M, Kandemir O, Demircan N. N-acetyl cysteine promotes angiogenesis and clearance of free oxygen radicals, thus improving wound healing in an alloxan-induced diabetic mouse model of incisional wound. Clin Exp Dermatol 35(2010) 902-909.
• 13. Roy S, Khanna S, Sen CK. Redox regulation of the VEGF signaling path and tissue vascularization: Hydrogen peroxide the common link between physical exercise and cutaneous wound healing. Free Radic Biol Med 44 (2008) 180-192.
• 14. Fraisl P. Crosstalk between oxygen- and nitric oxide-dependent signaling pathways in angiogenesis. Experimental Cell Research 319 (2013) 1331–1339.
• 15. İnan ZDŞ. Deneysel Diyabetli Sıçanlarda Kitozanın Yara İyileşmesine Etkisinin İncelenmesi. Yüksek Lisans Tezi. HistolojiEmbriyoloji AbD. Sivas Cumhuriyet Üniversitesi Fen/Sağlık Bilimleri Enstitüsü (2009)
• 16. Kurtel H, Granger DN, Tso P, Grisham MB. Vulnerability of intestinal interstitial fluid to oxidant stress. Am J Physiol 262 (1992) G573-578.
• 17. Elmann GL. Tissue sulyphydryl groups. Arch Biochem Biophys 82 (1959) 70-77.
• 18. Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5 (2001) 62-71.
• 19. Osman AE. Vasküler Endotelial Büyüme Faktörünün Diyabetik Yara İyileşmesi Üzerine Etkileri: Farelerde Deneysel Çalışmalar. Uzmanlık Tezi. Plastik Rekonstrüktif ve Estetik Cerrahi AbD. Dokuz Eylül Üniversitesi Tıp Fakültesi (2006)
• 20. Bao P, Kodra A, Tomic-Canic M, Golinko MS, Ehrlich HP, Brem H. The Role of Vascular Endothelial Growth Factor in Wound Healing. Journal of Surgical Research 153 (2008) 347–358.
• 21. Roy S, Khanna S, Nallu K, Hunt TK, Sen CK. Dermal wound healing is subject to redox control. Mol Ther 13 (2006) 211–220.
• 22. Jones DP, Go YM, Anderson CL, Ziegler TR, Kinkade JM, Kirlin WG. Cysteine/cystine couple is a newly recognized node in the circuitry for biologic redox signaling and control. FASEB J 18 (2004) 1246-1248.
• 23. Nathan C, Cunningham BA. Beyond oxidative stress: an immunologist's guide to reactive oxygen species. Nature Reviews Immunology 13 (2013) 349-361.
• 24. Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: how are they linked?. Free Radic Biol Med 49 (2010) 1603–1616.
• 25. Sönmez Çoban V, Coşkun Cevher Ş. The Investigation of the Effect of Topical Vascular Endothelial Growth Factor (VEGF) Administration on Serum Oxidative Parameters in Diabetic Rats. Gazi University Journal of Science 29 (2016) 543-547.
• 26. Stojadinovic O, Kodra A, Golinko MS, Tomic-Canic M, Brem H. A novel, non-angiogenic mechanism of VEGF: Stimulation of keratinocyte and fibroblast migration. Wound Repair Regen 15 (2007) 30.