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Year 2018, Volume: 5 Issue: 3, 225 - 230, 30.09.2018
https://doi.org/10.17350/HJSE19030000099

Abstract

References

  • 1. Guillou-Guillemette HL and Lunel-Fabiani F, Detection and quantification of serum or plasma HCV RNA: Mini review of commercially available assays, in hepatitis C: Methods and protocols. ed. H. Tang, Humana Press, Totowa, NJ, 2nd edn 150 (2009) 3–14.
  • 2. Zobair MY, Hepatitis C infection: A systemic disease, Clinics in Liver Disease 21 (2017) 449-453.
  • 3. Thomas JR and Hergenrother PJ, targeting RNA with small molecules. Chemical Reviews 108 (2008) 1171-1224.
  • 4. Li W, Wu P, Zhang H and Cai C, Catalytic signal amplification of gold nanoparticles combining with conformation-switched hairpin DNA probe for hepatitis C virus quantification. Chemical Communications 48 (2008) 7877–7879.
  • 5. Liu L, Wang X, Ma Q, Lin Z, Chen S, Li Y, Lu L, Qu H and Su X, Multiplex electrochemiluminescence DNA sensor for determination of hepatitis B virus and hepatitis C virus based on multicolor quantum dots and Au nanoparticles. Analytica Chimica Acta 916 (2016) 92-101.
  • 6. Skladal P, Riccardi CS, Yamanaka H and Costa PI, Piezoelectric biosensors for real-time monitoring of hybridization and detection of hepatitis C virus. Journal of Virological Methods 117 (2004) 145-151.
  • 7. Wenbing L, Xinchu Z, Zhifeng Y, Glushenkov AM, and Kong L, Plasmonic substrates for surface enhanced Raman scattering. Analytica Chimica Acta 984 (2017) 19-41.
  • 8. Cho I-H, Das M, Bhandari P, and Irudayaraj J, High performance immunochromatographic assay combined with surface enhanced Raman spectroscopy. Sensors and Actuators B: Chemical, 213 (2015) 209-214.
  • 9. Herrera G, Padilla A, and Rivera SH, Surface enhanced Raman scattering (SERS) studies of gold and silver nanoparticles prepared by laser ablation. Nanomaterials 3 (2013) 158-172.
  • 10. Stockman MI, Nanoplasmonics: past, present, and glimpse into future. Optics Express 19 (2011) 22029-22106.
  • 11. Pang Y, Wang J, Xiao R, Wang S, SERS molecular sentinel for the RNA genetic marker of PB1-F2 protein in highly pathogenic avian influenza (HPAI) virus. Biosensors and Bioelectronics 61 (2014) 460-465.
  • 12. Wang H-N, Fales AM, Zaas AK, Woods CW, Burke T, Ginsburg GS, Vo-Dinh T, Surface-enhanced Raman scattering molecular sentinel nanoprobes for viral infection diagnostics. Analytica Chimica Acta 786 (2013) 153-158.
  • 13. Tumturk H, Sahin F and Turan E, Magnetic nanoparticles coated with different shells for bio recognition high specific binding capacity. Analyst 139 (2014) 1093–1100.
  • 14. Haiss W, Thanh NTK, Aveyard J,and Fernig DG, Determination of size and concentration of gold nanoparticles from UV−Vis spectra. Analytical Chemistry 79 (2007) 4215–4221.
  • 15. Qin W, Song Z, Fan C, Zhang W, Cai Y, Zhang Y, and Qian X, Trypsin immobilization on hairy polymer chains hybrid magnetic nanoparticles for ultrafast, highly efficient proteome digestion, facile 18O labeling and absolute protein quantification. Analytical Chemistry 84 (2012) 3138–3144.
  • 16. Kouassi GK, and Irudayaraj J, Magnetic and gold-coated magnetic nanoparticles as a DNA sensor. Analytical Chemistry 78 (2006) 3234–3241.
  • 17. Griffin J, Singh AK, Senapati, Lee E, Gaylor K, Jones-Boone J. and Ray PC, Sequence-specific HCV RNA quantification using the size-dependent nonlinear optical properties of gold nanoparticles. Small 5 (2009) 5, 839.
  • 18. Liu S, Hu Y, Jin J, Zhang H and Cai CX, Electrochemical detection of hepatitis C virus based on site-specific DNA cleavage of BamHI endonuclease. Chemical Communications 2009, 1635-1637.
  • 19. Liu S, Wang Q, Chen D, Jin J, Hu Y, Wu P, Zhang H and Cai CX, Electrochemical approach for the specific detection of hepatitis C virus based on site-specific DNA cleavage of BamHI endonuclease. Analytical Methods 2 (2010) 135-142.

Preparation of Molecular Sentinel Based SERS Sensor for Hepatitis C Virus

Year 2018, Volume: 5 Issue: 3, 225 - 230, 30.09.2018
https://doi.org/10.17350/HJSE19030000099

Abstract

Construction of a rapid, cost-effective and label free biosensor is important issue for public health. In this study, it has been developed a sensitive, selective and simple biosensor for the detection of hepatitis C virus HCV DNA. For this purpose, firstly, superparamagnetic gold nanoparticles were prepared by simple citrate reduction method and used as surface enhanced Raman spectroscopy SERS substrate. Then, Raman labelled hairpin DNA molecular sentinel, MS was covalently bound on the gold shell by means of gold-sulfur interaction. After addition the complementary DNA target HCV DNA , the hairpin structure was changed closed state stem-loop configuration to open state hybridization configuration . As a result, the Raman label was located far away from the surface and reduced the SERS intensity. A good relationship was obtained between the decreasing of the SERS intensity and the target DNA concentration 0-50 pM and the limit of detection was found to be 0.1 pM. The sensing method only consists of a single hybridization and washing procedure after hybridization and centrifuge step can be omitted. It is believed that the prepared biosensor could be a powerful diagnostic tool for HCV detection.

References

  • 1. Guillou-Guillemette HL and Lunel-Fabiani F, Detection and quantification of serum or plasma HCV RNA: Mini review of commercially available assays, in hepatitis C: Methods and protocols. ed. H. Tang, Humana Press, Totowa, NJ, 2nd edn 150 (2009) 3–14.
  • 2. Zobair MY, Hepatitis C infection: A systemic disease, Clinics in Liver Disease 21 (2017) 449-453.
  • 3. Thomas JR and Hergenrother PJ, targeting RNA with small molecules. Chemical Reviews 108 (2008) 1171-1224.
  • 4. Li W, Wu P, Zhang H and Cai C, Catalytic signal amplification of gold nanoparticles combining with conformation-switched hairpin DNA probe for hepatitis C virus quantification. Chemical Communications 48 (2008) 7877–7879.
  • 5. Liu L, Wang X, Ma Q, Lin Z, Chen S, Li Y, Lu L, Qu H and Su X, Multiplex electrochemiluminescence DNA sensor for determination of hepatitis B virus and hepatitis C virus based on multicolor quantum dots and Au nanoparticles. Analytica Chimica Acta 916 (2016) 92-101.
  • 6. Skladal P, Riccardi CS, Yamanaka H and Costa PI, Piezoelectric biosensors for real-time monitoring of hybridization and detection of hepatitis C virus. Journal of Virological Methods 117 (2004) 145-151.
  • 7. Wenbing L, Xinchu Z, Zhifeng Y, Glushenkov AM, and Kong L, Plasmonic substrates for surface enhanced Raman scattering. Analytica Chimica Acta 984 (2017) 19-41.
  • 8. Cho I-H, Das M, Bhandari P, and Irudayaraj J, High performance immunochromatographic assay combined with surface enhanced Raman spectroscopy. Sensors and Actuators B: Chemical, 213 (2015) 209-214.
  • 9. Herrera G, Padilla A, and Rivera SH, Surface enhanced Raman scattering (SERS) studies of gold and silver nanoparticles prepared by laser ablation. Nanomaterials 3 (2013) 158-172.
  • 10. Stockman MI, Nanoplasmonics: past, present, and glimpse into future. Optics Express 19 (2011) 22029-22106.
  • 11. Pang Y, Wang J, Xiao R, Wang S, SERS molecular sentinel for the RNA genetic marker of PB1-F2 protein in highly pathogenic avian influenza (HPAI) virus. Biosensors and Bioelectronics 61 (2014) 460-465.
  • 12. Wang H-N, Fales AM, Zaas AK, Woods CW, Burke T, Ginsburg GS, Vo-Dinh T, Surface-enhanced Raman scattering molecular sentinel nanoprobes for viral infection diagnostics. Analytica Chimica Acta 786 (2013) 153-158.
  • 13. Tumturk H, Sahin F and Turan E, Magnetic nanoparticles coated with different shells for bio recognition high specific binding capacity. Analyst 139 (2014) 1093–1100.
  • 14. Haiss W, Thanh NTK, Aveyard J,and Fernig DG, Determination of size and concentration of gold nanoparticles from UV−Vis spectra. Analytical Chemistry 79 (2007) 4215–4221.
  • 15. Qin W, Song Z, Fan C, Zhang W, Cai Y, Zhang Y, and Qian X, Trypsin immobilization on hairy polymer chains hybrid magnetic nanoparticles for ultrafast, highly efficient proteome digestion, facile 18O labeling and absolute protein quantification. Analytical Chemistry 84 (2012) 3138–3144.
  • 16. Kouassi GK, and Irudayaraj J, Magnetic and gold-coated magnetic nanoparticles as a DNA sensor. Analytical Chemistry 78 (2006) 3234–3241.
  • 17. Griffin J, Singh AK, Senapati, Lee E, Gaylor K, Jones-Boone J. and Ray PC, Sequence-specific HCV RNA quantification using the size-dependent nonlinear optical properties of gold nanoparticles. Small 5 (2009) 5, 839.
  • 18. Liu S, Hu Y, Jin J, Zhang H and Cai CX, Electrochemical detection of hepatitis C virus based on site-specific DNA cleavage of BamHI endonuclease. Chemical Communications 2009, 1635-1637.
  • 19. Liu S, Wang Q, Chen D, Jin J, Hu Y, Wu P, Zhang H and Cai CX, Electrochemical approach for the specific detection of hepatitis C virus based on site-specific DNA cleavage of BamHI endonuclease. Analytical Methods 2 (2010) 135-142.
There are 19 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Adem Zengin This is me

Publication Date September 30, 2018
Published in Issue Year 2018 Volume: 5 Issue: 3

Cite

Vancouver Zengin A. Preparation of Molecular Sentinel Based SERS Sensor for Hepatitis C Virus. Hittite J Sci Eng. 2018;5(3):225-30.

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