Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro

Year 2018, Volume: 5 Issue: 1, 1 - 5, 28.03.2018

Sükran Seker

https://doi.org/10.17350/HJSE19030000070

Abstract

Zinc oxide ZnO has been widely used in wound dressing, food additive and root canal filling material owing to its antibacterial and antifungal properties. This study describes potential cytotoxic effect of ZnO on human periodontal ligament fibroblasts hPDLFs in vitro. ZnO was characterized using BET Braun-Emmet-Teller and DLS dynamic light scattering analyses. The hPDLFs were treated with 0.1, 1, 10, 50 and 100 μg/mL of ZnO for 6 h, 24 h and 48 h durations. After exposure to ZnO, phase contrast microscopy, transmission electron microscopy TEM , mitochondrial function MTT and lactate dehydrogenase LDH assays were used to evaluate cell morphology, particle uptake, cell viability and membrane leakage. In addition, real time cell responses to ZnO exposure were monitored by impedance measurements. Results indicated that ZnO exposure caused toxic effect in a dose- and timedependent manner when compared to control group, especially at 50 and 100 μg/mL.

Keywords

Cytotoxicity, Zinc oxide, Periodontal ligament fibroblasts, Impedance, In vitro toxicity assays

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