The cytologic investigation of brow adipose tissue of D.laniger Felten & Storch, 1968 Mammalia: Rodentia in hibernation

Year 2015, Volume: 2 Issue: 2, 187 - 191, 30.12.2015

Aydın Ozluk Diler Salman Dilek Yilmaz Ahmet Cilak Nursel Gül Menderes Suicmez

https://doi.org/10.17350/HJSE19030000024

Abstract

T his study was conducted on the D.laniger samples maintained in the laboratory condition after collecting from the natural environment. Investigations were performed in the experimental groups in the active period at the time of hibernation and at the period of intermediate awakening during hibernation. The brown adipose tissue BAT of these animals investigated was removed by dissection. The tissue samples dissected were prepared for the analysis at Transmission Electron Microscopy TEM and photographed. It was observed for the D. laniger BAT cells in the active period that there are high amount mitochondria and whereas the scarce amount of the lipid droplets. However, it was drawn attention to BAT cell of animals in hibernation that there plenty of capillary vessels and lipid droplets between them. The contact between lipid droplets with each other in the cytoplasm of animal at intermediate awakening in the winter during hibernation and cytoplasmic material loss and melting of mitochondria cristae partly were observed. These results of this study suggest that it may be useful to understand the importance of the brown adipose tissue of some hibernating mammals

Keywords

Dryomys Laniger, Brown Adipose Tissue, Mitochondria, Hibernation.

References

• Wade O. The behaviour of certain spermophiles with special references to estivation and hibernation. J. Mamm. 11 (1930) 160-188.
• Barnes BM. Freeze avoidance in a mammal body temperatures below 0oC in an Arctic hibernator. Sci. 244 (1989) 1593-1595.
• 12. Geiser F. Metabolic rate and body temparature reduction during hibernation and daily torpor. First published 2003. Annu. Rev. Physiol. 66 (2004) 239-274.
• Çiftçi N. Tiroksinin oda ısısında sıçan skapular arası kahverengi yağ dokusuna etkisinin elektron mikroskobu düzeyinde incelenmesi. Ondokuz Mayıs Üniversitesi Tıp Fakültesi Dergisi, 6(1989a) 465-472.
• Suter ER. The fine structure of brown adipose tissue. I. Coldinducen changes in the rat. J. Ultrastruct Research. 26 (1969) 216-241.
• Altunkaynak BZ and Özbek E. Yağ dokusu endokrin bir organ mıdır? Dicle Tıp Dergisi. 32 (2005) 211-217.
• Revel JP and Sheridan JD. Electrophysiological and ultrastructural studies of intercellular function in brown fat. The Jornual of Physiology. 194 (1968) 34-35.
• Lindberg O. Brown adipose tissue. American Elsevier Pub. Co., New York , USA, (1970) 337.
• Watanabe J, Kanamura S, Tokunaga H, Sakaida M and Kana K. Significance of increase in glucose 6-phosphatase activity in brown adipose cells of cold-exposed and starved mice. The Anatomical Record. 219 (1987) 39-44.
• Sternberg S. Brown Adipose Tissue. Second edition histology for pathologist. Ed: Stephen S. Sternberg. Lippincott-Raven publishers. (1997) 173-175.
• Wang LCH and Abbots B. Maximum thermogenesis in hibernators: magnitudes and seasonal variations. In: Musacchia XJ and Jansky L (eds). Survival in the cold. Elsevier, Amsterdam. (1981) 77-97.
• Himms-Hagen J. Brown adipose tissue and cold acclimatation. In: Trayhurn P and Nicholls DG (eds). Brown adipose tissue. Edward Arnold, London. (1986) 214-268.
• Kolaeva SG, Kramarova LI, Ilyasova FE. The kinetics and metabolism of the cells of hibernating animals during hibernation. Int. Rev. Cytol. 66 (1980) 148-169.
• Zancanaro C, Malatesta M, Merigo F, Benati D, Fakan S and Gazzanelli G. Ultrastructure of organs and tissue of dormice during hibernation. In: Heldmaier G and Klingenspor M (eds). Life in the cold, Springer, Berlin. (2000) 269-276.
• Akay MT. Genel Histoloji. Palme Yayıncılık, Ankara (1999) 200.
• Kramarova LI, Bronnikov GE, Ignat’ev DA, Cannon B and Nedergaard J. Adrenergic receptor density in brown adipose tissue of active and hibernating hamsters and ground squirrels. Comparative Biochemisrty and Physiology, Part A. (2007) 408-414.
• Malatesta M, Fakan S, Zancanaro C. Cell and tissue structural modifications in hibernating dormice. Hystrix It. J. Mamm. 16 (2005) 41-52.
• Göney G. D.laniger (Felten & Storch, 1968) (Mammalia: Rodentia)’in davranışsal açıdan hibernasyonunun incelenmesi. Yayınlanmamış Yüksek Lisans Tezi, Hitit Üniversitesi Fen Bilimleri Enstitüsü, Çorum. (2011).
• Vaughan DK, Gruber AR, Michalski ML, Seidling J, Schlink S. Capture, care, and captive breeding of 13-lined ground squirrels, Spermophilus tridecemlineatus. Lab Animal. 35 (2006) 33-40.
• Horwitz BA, Hamilton JS and Kott KS. GDP binding to hamster brown fat mitochondria is reduced during hibernation. Am. J. Physiol. 249 (1985) 689-693.
• Andrews JF, Richard D, Jennings G and Trayhurn P. Annals of nutrition and metabolism. 30 (1986).
• Çiftçi N. Tiroksinin soğuk ortamda sıçan skapular arası kahverengi yağ dokusuna etkisinin elektron mikroskobu düzeyinde incelenmesi. Ondokuz Mayıs Üniversitesi Tıp Fakültesi Dergisi. 6(1989b) 373-383.