Year 2021, Volume 8 , Issue 1, Pages 35 - 40 2021-03-31

Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans

Adem ÖZÇELİK [1]


Model organisms such as Caenorhabditis elegans are commonly used in discovering theunderlying mechanisms of human diseases. The field of microfluidics enables a plethora of tools for better studying these organisms. In this research, an effective and simple approach is demonstrated for thermally induced knockdown of Caenorhabditis elegans model organisms. To achieve this, surface acoustic waves are coupled to the fluid medium inside a microfluidic channel. Without active cooling, two pairs of propagating surface acoustic waves induce very rapid heating of the enclose fluid. By controlling the input power of the acoustic waves, temperature of the fluid is precisely adjusted, and induced reversible knockdown of Caenorhabditis elegans samples. Effective temperature window of acoustically induced thermal knockdown is characterized, and the tolerable number of knockdown and wake up cycles is studied. Viability of the Caenorhabditis elegans organisms is also evaluated after increasing numbers of knockdown cycles. Overall, a simple and effective method of worm immobilization method is demonstrated using acoustic waves in microfluidics.
C. elegans, İmmobilization, Microfluidics, Surface acoustic waves, Thermoacoustics
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Primary Language en
Subjects Engineering
Journal Section Research Article
Authors

Orcid: 0000-0002-3124-795X
Author: Adem ÖZÇELİK
Institution: AYDIN ADNAN MENDERES UNIVERSITY
Country: Turkey


Dates

Application Date : October 4, 2020
Acceptance Date : February 4, 2021
Publication Date : March 31, 2021

Bibtex @research article { hjse907471, journal = {Hittite Journal of Science and Engineering}, issn = {}, eissn = {2148-4171}, address = {Hitit Üniversitesi Mühendislik Fakültesi Kuzey Kampüsü Çevre Yolu Bulvarı 19030 Çorum / TÜRKİYE}, publisher = {Hitit University}, year = {2021}, volume = {8}, pages = {35 - 40}, doi = {10.17350/HJSE19030000210}, title = {Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans}, key = {cite}, author = {Özçelik, Adem} }
APA Özçelik, A . (2021). Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans . Hittite Journal of Science and Engineering , 8 (1) , 35-40 . DOI: 10.17350/HJSE19030000210
MLA Özçelik, A . "Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans" . Hittite Journal of Science and Engineering 8 (2021 ): 35-40 <https://dergipark.org.tr/en/pub/hjse/issue/61101/907471>
Chicago Özçelik, A . "Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans". Hittite Journal of Science and Engineering 8 (2021 ): 35-40
RIS TY - JOUR T1 - Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans AU - Adem Özçelik Y1 - 2021 PY - 2021 N1 - doi: 10.17350/HJSE19030000210 DO - 10.17350/HJSE19030000210 T2 - Hittite Journal of Science and Engineering JF - Journal JO - JOR SP - 35 EP - 40 VL - 8 IS - 1 SN - -2148-4171 M3 - doi: 10.17350/HJSE19030000210 UR - https://doi.org/10.17350/HJSE19030000210 Y2 - 2021 ER -
EndNote %0 Hittite Journal of Science and Engineering Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans %A Adem Özçelik %T Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans %D 2021 %J Hittite Journal of Science and Engineering %P -2148-4171 %V 8 %N 1 %R doi: 10.17350/HJSE19030000210 %U 10.17350/HJSE19030000210
ISNAD Özçelik, Adem . "Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans". Hittite Journal of Science and Engineering 8 / 1 (March 2021): 35-40 . https://doi.org/10.17350/HJSE19030000210
AMA Özçelik A . Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans. Hittite J Sci Eng. 2021; 8(1): 35-40.
Vancouver Özçelik A . Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans. Hittite Journal of Science and Engineering. 2021; 8(1): 35-40.
IEEE A. Özçelik , "Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans", Hittite Journal of Science and Engineering, vol. 8, no. 1, pp. 35-40, Mar. 2021, doi:10.17350/HJSE19030000210