Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans

Year 2021, Volume: 8 Issue: 1, 35 - 40, 31.03.2021

Adem Özçelik

https://doi.org/10.17350/HJSE19030000210

Cited By: 1

Abstract

Model organisms such as Caenorhabditis elegans are commonly used in discovering theunderlying mechanisms of human diseases. The field of microfluidics enables a plethora of tools for better studying these organisms. In this research, an effective and simple approach is demonstrated for thermally induced knockdown of Caenorhabditis elegans model organisms. To achieve this, surface acoustic waves are coupled to the fluid medium inside a microfluidic channel. Without active cooling, two pairs of propagating surface acoustic waves induce very rapid heating of the enclose fluid. By controlling the input power of the acoustic waves, temperature of the fluid is precisely adjusted, and induced reversible knockdown of Caenorhabditis elegans samples. Effective temperature window of acoustically induced thermal knockdown is characterized, and the tolerable number of knockdown and wake up cycles is studied. Viability of the Caenorhabditis elegans organisms is also evaluated after increasing numbers of knockdown cycles. Overall, a simple and effective method of worm immobilization method is demonstrated using acoustic waves in microfluidics.

Keywords

C. elegans, İmmobilization, Microfluidics, Surface acoustic waves, Thermoacoustics

References

• Kaletta, T., Hengartner, M.O. Finding function in novel targets: C. elegans as a model organism. Nature Reviews. Drug Discovery. 5(5) (2006) 387–98. doi: 10.1038/nrd2031.
• Rohde, C.B., Zeng, F., Gonzalez-Rubio, R., Angel, M., Yanik, M.F. Microfluidic system for on-chip high-throughput whole-animal sorting and screening at subcellular resolution. Proceedings of the National Academy of Sciences of the United States of America. 104(35) (2007) 13891–5. doi: 10.1073/pnas.0706513104.
• Gilleland, C.L., Rohde, C.B., Zeng, F., Yanik, M.F. Microfluidic immobilization of physiologically active Caenorhabditis elegans. Nature Protocols. 5(12) (2010) 1888–902. doi: 10.1038/nprot.2010.143.
• Cáceres, I. de C., Valmas, N., Hilliard, M.A., Lu, H. Laterally orienting C. elegans using geometry at microscale for high-throughput visual screens in neurodegeneration and neuronal development studies. PLoS ONE. 7(4) (2012) e35037. doi: 10.1371/journal.pone.0035037.
• Tosun, E., Ozgur, T., Ozgur, C., Ozcanli, M., Serin, H., Aydin, K. Comparative analysis of various modelling techniques for emission prediction of diesel engine fueled by diesel fuel with nanoparticle additives. European Mechanical Science. 1(1) (2017) 15–23. doi: 10.26701/ems.320490.
• Akmeşe, B., Asan, A. Optimization of the Schiff-Base Reaction of Acetylacetone with Biogenic Amines. Hittite Journal of Science and Engineering. 4(1) (2017) 79–83. doi: 10.17350/HJSE19030000052.
• Erdoğan, B., Topuz, A., Engin, T., Baş, A., Yeter, A. Numerical and Experimental Investigation of the Effect on Heat Transfer of Nanofluid Usage in Mini/Micro Channels. Hittite Journal of Science & Engineering. 5 (2018) doi: 10.17350/HJSE19030000113.
• Orbay, S., Ozcelik, A., Bachman, H., Huang, T.J.T.J. Acoustic actuation of in situ fabricated artificial cilia. Journal of Micromechanics and Microengineering. 28(2) (2018) 025012. doi: 10.1088/1361-6439/aaa0ae.
• Ayan, B., Ozcelik, A., Bachman, H., Tang, S.-Y.S.-Y., Xie, Y., Wu, M., et al. Acoustofluidic coating of particles and cells. Lab Chip. 16(22) (2016) 4366–72. doi: 10.1039/C6LC00951D.
• Ozcelik, A., Nama, N., Huang, P.-H.H., Kaynak, M., McReynolds, M.R.M.R.M.R., Hanna-Rose, W., et al. Acoustofluidic Rotational Manipulation of Cells and Organisms Using Oscillating Solid Structures. Small. 12(37) (2016) 5120–5. doi: 10.1002/smll.201601760.
• Ahmed, D., Ozcelik, A., Bojanala, N., Nama, N., Upadhyay, A., Chen, Y., et al. Rotational manipulation of single cells and organisms using acoustic waves. Nature Communications. 7 (2016) 1–11. doi: 10.1038/ncomms11085.
• Gupta, B., Rezai, P. Microfluidic Approaches for Manipulating, Imaging, and Screening C. elegans. Micromachines. 7(7) (2016) 123. doi: 10.3390/mi7070123.
• Ben-Yakar, A., Chronis, N., Lu, H. Microfluidics for the analysis of behavior, nerve regeneration, and neural cell biology in C. elegans. Current Opinion in Neurobiology. 19(5) (2009) 561–7. doi: 10.1016/j.conb.2009.10.010.
• Cheng, C.M., LeDuc, P.R., Lin, Y.W. Localized bimodal response of neurite extensions and structural proteins in dorsal-root ganglion neurons with controlled polydimethylsiloxane substrate stiffness. Journal of Biomechanics. 44(5) (2011) 856–62. doi: 10.1016/j.jbiomech.2010.12.006.
• Massie, M.R., Lapoczka, E.M., Boggs, K.D., Stine, K.E., White, G.E. Exposure to the metabolic inhibitor sodium azide induces stress protein expression and thermotolerance in the nematode Caenorhabditis elegans. Cell Stress & Chaperones. 8(1) (2003) 1. doi: 10.1379/1466-1268(2003)8<1:ETTMIS>2.0.CO;2.
• Yanik, M.F., Cinar, H., Cinar, H.N., Chisholm, A.D., Jin, Y., Ben-Yakar, A. Neurosurgery: functional regeneration after laser axotomy. Nature. 432(7019) (2004) 822. doi: 10.1038/432822a.
• Garrity, P.A., Goodman, M.B., Samuel, A.D., Sengupta, P. Running hot and cold: behavioral strategies, neural circuits, and the molecular machinery for thermotaxis in C. elegans and Drosophila. Genes & Development. 24(21) (2010) 2365–82. doi: 10.1101/gad.1953710.
• Chuang, H.-S., Chen, H.-Y., Chen, C.-S., Chiu, W.-T. Immobilization of the nematode caenorhabditis elegans with addressable light-induced heat knockdown (ALINK). Lab on a Chip. 13(15) (2013) 2980. doi: 10.1039/c3lc50454a.
• Zhang, P., Bachman, H., Ozcelik, A., Huang, T.J. Acoustic Microfluidics. Annual Review of Analytical Chemistry. 13(1) (2020) 17–43. doi: 10.1146/annurev-anchem-090919-102205.
• Nawaz, A.A., Chen, Y., Nama, N., Nissly, R.H.R.H., Ren, L., Ozcelik, A., et al. Acoustofluidic Fluorescence Activated Cell Sorter. Analytical Chemistry. 87(24) (2015) 12051–8. doi: 10.1021/acs.analchem.5b02398.
• Ozcelik, A., Ahmed, D., Xie, Y., Nama, N., Qu, Z., Nawaz, A.A.A.A., et al. An Acoustofluidic Micromixer via Bubble Inception and Cavitation from Microchannel Sidewalls. Analytical Chemistry. 86(10) (2014)