Year 2021, Volume 8 , Issue 2, Pages 141 - 147 2021-06-30

Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content

Kaan KALTALIOĞLU [1] , Şule Coşkun CEVHER [2]


Isatis L. genus highly endemic and that is traditionally suggested for wound management in Turkey. In this study, we have reported the effect of I. glauca subsp. sivasica extract on diabetic wound healing process. A diabetic model was generated in Wistar rats using streptozotocin injection. Rats were divided into two main groups: control group and Isatis group. Full thickness excisional skin wounds were created by using a biopsy punch. The Isatis group was treated with single daily dose I. glauca subsp. sivasica extract (50 mg/kg). Rats were sacrificed day 3 or 7 after wounding. The dominant phenolic compounds identified with RP-HPLC-DAD from Isatis extract were the benzoic acid and vanillic acid. Isatis extract significantly accelerated wound healing process considering the wound closure rates (WCR). Moreover, the levels of collagen and nitric oxide were elevated on days 3 and 7 by Isatis administration in the diabetic wound tissue. These data suggest that, for the first time, I. glauca subsp. sivasica extract may have the potential to promote the impaired wound healing in patients with diabetes.
collagen, diabetes mellitus, Isatis glauca subsp. sivasica, nitric oxide, wound healing
  • Güner A, Aslan S, Ekim T, Vural M, Babaç MT. Türkiye Bitkileri Listesi (Damarlı Bitkiler), first ed. Flora Araştırmaları Derneği ve Nezahat Gökyiğit Botanik Bahçesi Yayını, İstanbul, 2012.
  • Yıldız N, Karakaş Ö. Qualitative and Quantitative Determination of Tryptanthrin, Indirubin, Indican and Isatin Indole Alkaloids During Vegetative and Flowering Stages in the Roots and Leaves of Isatis constricta P. H. Davis. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi 28 (2019) 59–66.
  • Karakoca K, Özüsağlam MA, Çakmak YS, Erkul SK. Antioxidative, antimicrobial and cytotoxic properties of isatis floribunda boiss. ex bornm. Extracts. EXCLI Journal 12 (2013) 150–167.
  • Küpeli E, Orhan İ, Yeşilada E. Evaluation of Some Plants Used in Turkish Folk Medicine for Their Anti-inflammatory and Antinociceptive Activities. Pharmaceutical Biology 45 (2007) 547–555.
  • Hamburger M. Isatis tinctoria - From the Rediscovery of an Ancient Medicinal Plant Towards a Novel Anti-Inflamatory Phytopharmaceutical. Phytochemistry Reviews 1 (2002) 333–344.
  • Tuzlacı E, Doğan A. Turkish folk medicinal plants, IX: Ovacık (Tunceli). Marmara Pharmaceutical Journal 14 (2010) 136–143.
  • Rezaeipoor R, Ziaei Sanei L, Kamalinejad M. Effects of Isatis cappadocica on humoral immune responses. Fitoterapia 71 (2000) 14-18.
  • Ozturk M, Uysal I, Gucel S, Altundag E, Dogan Y, Baslar S. Medicinal Uses of Natural Dye-Yielding Plants in Turkey. Research Journal of Textile and Apparel 17 (2013) 69–80.
  • Baytop T. Therapy with Plants in Turkey (Past & Present), second ed. Nobel Tıp, İstanbul, 1999.
  • Guo S, DiPietro LA. Factors Affecting Wound Healing. Journal of Dental Research 89 (2010) 219–229.
  • Li H, Fu X, Zhang L, Huang Q, Wu Z, Sun T. Research of PDGF-BB gel on the wound healing of diabetic rats and its pharmacodynamics. J Surg Res 145 (2008) 41–48.
  • Kaltalioglu K, Balabanli B, Coskun-Cevher S. Phenolic, Antioxidant, Antimicrobial, and In-vivo Wound Healing Properties of Potentilla erecta L. Root Extract in Diabetic Rats. Iranian Journal of Pharmaceutical Research 19 (2020) 264–274.
  • Brett D. A review of collagen and collagen-based wound dressings. Wounds 20 (2008) 347–356.
  • Schäfer M, Werner S. Oxidative stress in normal and impaired wound repair. Pharmacol Res 58 (2008) 165–171.
  • Blakytny R, Jude E. The molecular biology of chronic wounds and delayed healing in diabetes. Diabet Med 23 (2006) 594–608.
  • Malone-Povolny MJ, Maloney SE, Schoenfisch MH. Nitric Oxide Therapy for Diabetic Wound Healing. Adv Healthc Mater 8 (2019) e1801210.
  • Bektas E, Kaltalioglu K, Sahin H, Turkmen Z, Kandemir A. Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plants. Int J Sec Metabolite 5 (2018) 75–86.
  • Tsuda K, Nakatani T, Sugama J, Okuwa M, Sanada H. Influence of the timing of switching a protein-free to a protein-containing diet on the wound healing process in a rat all-layer skin defect. Int Wound J 7 (2010) 135–146.
  • Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5 (2001) 62–71.
  • Miceli N, Filocamo A, Ragusa S, Cacciola F, Dugo P, Mondello L, Celano M, Maggisano V, Taviano MF. Chemical characterization and biological activities of phenolic-rich fraction from cauline leaves of Isatis tinctoria L. (Brassicaceae) growing in Sicily, Italy. Chem Biodivers 14 (2017) e1700073.
  • dos Santos Gramma LS, Marques FM, Vittorazzi C, de Andrade, TAM, Frade MAC, de Andrade TU, Endringer DC, Scherer R, Fronza M. Struthanthus vulgaris ointment prevents an over expression of inflammatory response and accelerates the cutaneous wound healing. Journal of ethnopharmacology 190 (2016) 319–327.
  • Kumar S, Prahalathan P, Raja B. Antihypertensive and antioxidant potential of vanillic acid, a phenolic compound in l-NAME-induced hypertensive rats: A dose-dependence study. Redox Report 16 (2011) 208–215.
  • Ugusman A, Zakaria Z, Chua KH, Nordin NAMM, Abdullah Mahdy Z. Role of rutin on nitric oxide synthesis in human umbilical vein endothelial cells. Scientific World Journal (2014) 2014: 1–9.
  • Choi SJ, Lee SN, Kim K, Joo da H, Shin S, Lee J, Lee HK, Kim J, Kwon SB, Kim MJ, Ahn KJ, An IS, An S, Cha HJ. Biological effects of rutin on skin aging. Int J Mol Med 38 (2016) 357–363.
  • Kızıl S, Turk M, Çakmak Ö, Özgüven M, Khawar KM. Microelement Contents and Fatty Acid Compositions of some Isatis Species Seeds. Not Bot Horti Agrobo 37 (2009) 175–178.
  • Lambertucci RH, Leandro CG, Vinolo MA, Nachbar RT, Dos Reis Silveira L, Hirabara SM, Curi R, Pithon-Curi TC. The effects of palmitic acid on nitric oxide production by rat skeletal muscle: mechanism via superoxide and iNOS activation. Cell Physiol Biochem 30 (2012) 1169–1180.
  • de Lima TM, de Sa Lima L, Scavone C, Curi R. Fatty acid control of nitric oxide production by macrophages. FEBS Lett 580 (2006) 3287–3295.
  • Witte MB, Thornton FJ, Efron DT, Barbul A. Enhancement of fibroblast collagen synthesis by nitric oxide. Nitric Oxide 4 (2000) 572–582.
  • Witte MB, Kiyama T, Barbul A. Nitric oxide enhances experimental wound healing in diabetes. Br J Surg 89 (2002) 1594–1601.
  • Achuth HN, Moochhala SM, Mahendran R, Tan WT. Nitrosoglutathione triggers collagen deposition in cutaneous wound repair. Wound Repair Regen 13 (2005) 383–389.
  • Luo J, Chen AF. Nitric oxide: a newly discovered function on wound healing. Acta Pharmacol Sin 26 (2005) 259–264.
Primary Language en
Subjects Basic Sciences
Journal Section Research Article
Authors

Orcid: 0000-0002-4995-2657
Author: Kaan KALTALIOĞLU (Primary Author)
Institution: Giresun Üniversitesi
Country: Turkey


Orcid: 0000-0001-6204-2845
Author: Şule Coşkun CEVHER
Institution: Gazı University
Country: Turkey


Dates

Application Date : February 25, 2021
Acceptance Date : May 23, 2021
Publication Date : June 30, 2021

Bibtex @research article { hjse886520, journal = {Hittite Journal of Science and Engineering}, issn = {}, eissn = {2148-4171}, address = {Hitit Üniversitesi Mühendislik Fakültesi Kuzey Kampüsü Çevre Yolu Bulvarı 19030 Çorum / TÜRKİYE}, publisher = {Hitit University}, year = {2021}, volume = {8}, pages = {141 - 147}, doi = {10.17350/HJSE19030000224}, title = {Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content}, key = {cite}, author = {Kaltalıoğlu, Kaan and Cevher, Şule Coşkun} }
APA Kaltalıoğlu, K , Cevher, Ş . (2021). Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content . Hittite Journal of Science and Engineering , 8 (2) , 141-147 . DOI: 10.17350/HJSE19030000224
MLA Kaltalıoğlu, K , Cevher, Ş . "Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content" . Hittite Journal of Science and Engineering 8 (2021 ): 141-147 <https://dergipark.org.tr/en/pub/hjse/issue/63382/886520>
Chicago Kaltalıoğlu, K , Cevher, Ş . "Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content". Hittite Journal of Science and Engineering 8 (2021 ): 141-147
RIS TY - JOUR T1 - Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content AU - Kaan Kaltalıoğlu , Şule Coşkun Cevher Y1 - 2021 PY - 2021 N1 - doi: 10.17350/HJSE19030000224 DO - 10.17350/HJSE19030000224 T2 - Hittite Journal of Science and Engineering JF - Journal JO - JOR SP - 141 EP - 147 VL - 8 IS - 2 SN - -2148-4171 M3 - doi: 10.17350/HJSE19030000224 UR - https://doi.org/10.17350/HJSE19030000224 Y2 - 2021 ER -
EndNote %0 Hittite Journal of Science and Engineering Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content %A Kaan Kaltalıoğlu , Şule Coşkun Cevher %T Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content %D 2021 %J Hittite Journal of Science and Engineering %P -2148-4171 %V 8 %N 2 %R doi: 10.17350/HJSE19030000224 %U 10.17350/HJSE19030000224
ISNAD Kaltalıoğlu, Kaan , Cevher, Şule Coşkun . "Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content". Hittite Journal of Science and Engineering 8 / 2 (June 2021): 141-147 . https://doi.org/10.17350/HJSE19030000224
AMA Kaltalıoğlu K , Cevher Ş . Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. Hittite J Sci Eng. 2021; 8(2): 141-147.
Vancouver Kaltalıoğlu K , Cevher Ş . Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. Hittite Journal of Science and Engineering. 2021; 8(2): 141-147.
IEEE K. Kaltalıoğlu and Ş. Cevher , "Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content", Hittite Journal of Science and Engineering, vol. 8, no. 2, pp. 141-147, Jun. 2021, doi:10.17350/HJSE19030000224