Research Article
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Year 2021, Volume: 8 Issue: 2, 171 - 177, 30.06.2021
https://doi.org/10.17350/HJSE19030000227

Abstract

References

  • 1. Hoshiba T, Lu H, Kawazoe N, Chen G. Decellularized matrices for tissue engineering. Expert Opinion on Biological Therapy 10(12) (2010) 1717-1728.
  • 2. Lalegül‐Ülker Ö, Vurat MT, Elçin AE, Elçin YM. Magnetic silk fibroin composite nanofibers for biomedical applications: Fabrication and evaluation of the chemical, thermal, mechanical, and in vitro biological properties. Journal of Applied Polymer Science 136(41) (2019) 48040.
  • 3. Vurat MT, Elçin AE, Elçin YM. Osteogenic composite nanocoating based on nanohydroxyapatite, strontium ranelate and polycaprolactone for titanium implants. Transactions of Nonferrous Metals Society of China 28(9) (2018) 1763-1773.
  • 4. Parmaksiz M, Elçin AE, Elçin YM. Decellularized Cell Culture ECMs Act as Cell Differentiation Inducers. Stem Cell Reviews and Reports 16(3) (2020) 569-584.
  • 5. Parmaksiz M, Elçin AE, Elçin YM. Decellularization of bovine small intestinal submucosa and its use for the healing of a critical‐sized full‐thickness skin defect, alone and in combination with stem cells, in a small rodent model. Journal of Tissue Engineering and Regenerative Medicine 11(6) (2017) 1754-1765.
  • 6. Parmaksiz M, Dogan A, Odabas S, Elçin AE, Elçin YM. Clinical applications of decellularized extracellular matrices for tissue engineering and regenerative medicine. Biomedical Materials 11(2) (2016) 022003.
  • 7. Parmaksiz M, Elçin AE, Elçin YM. Decellularized bovine small intestinal submucosa-PCL/hydroxyapatite-based multilayer composite scaffold for hard tissue repair. Materials Science and Engineering C 94 (2019) 788-797
  • 8. Parmaksiz M, Elçin AE, Elçin YM. Decellularization of Bovine Small Intestinal Submucosa. In: Turksen K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, Humana Press, New York, pp. 129-138, 2018.
  • 9. Baptista PM, Siddiqui MM, Lozier G, Rodriguez SR, Atala A, Soker S. The use of whole organ decellularization for the generation of a vascularized liver organoid. Hepatology 53(2) (2011) 604-617.
  • 10. Skardal A., Smith L, Bharadwaj S, Atala A, Soker S, Zhang Y. Tissue specific synthetic ECM hydrogels for 3-D in vitro maintenance of hepatocyte function. Biomaterials 33(18) (2012). 4565–4575.
  • 11. Ren H, Shi X, Tao L, Xiao J, Han B, Zhang Y, Yuan X, Ding Y. Evaluation of two decellularization methods in the development of a whole-organ decellularized rat liver scaffold. Liver International 33(3) (2013) 448-458.
  • 12. Tajima K, Kuroda K, Otaka Y, Kinoshita R, Kita M, Oyamada T, Kanai K. Decellularization of canine kidney for three-dimensional organ regeneration. Veterinary World 13(3) (2020) 452-457.
  • 13. Lewis PL, Yan M, Su J. Shah RN. Directing the growth and alignment of biliary epithelium within extracellular matrix hydrogels. Acta Biomaterialia 85 (2019) 84-93.
  • 14. Mochane MJ, Motsoeneng TS, Sadiku ER, Mokhena TC, Sefadi JS. Morphology and Properties of Electrospun PCL and Its Composites for Medical Applications: A Mini Review. Applied Sciences 9(11) (2019) 2205.
  • 15. Schueren LVD, Schoenmaker BD, Kalaoglu OI, Clerk KD. An alternative solvent system for the steady state electrospinning of polycarbonate. European Polymer Journal 47 (2011) 1256–1263.
  • 16. Luk JZ, Cooper-White J, Rintoul L, Taran E, Grøndahl L. Functionalised polycaprolactone films and 3D scaffolds via gamma irradiation-induced grafting. Journal of Materials Chemistry B 1(33) (2013) 4171-4181.
  • 17. Janarthanan G, Kim IG, Chung EJ, Noh I. Comparative studies on thin polycaprolactone-tricalcium phosphate composite scaffolds and its interaction with mesenchymal stem cells. Biomaterials Research 23(1) (2019) 1-12.
  • 18. Wang X, Ding B, Li B. Biomimetic electrospun nanofibrous structures for tissue engineering. Materials Today 16(6) (2013) 229-241.
  • 19. Moursi AM, Damsky,CH, Lull J, Zimmerman D, Doty SB, Aota S, Globus RK. Fibronectin regulates calvarial osteoblast differentiation. Journal of Cell Science 109 (1996) 1369-1380.
  • 20. Rosso F, Giordano A, Barbarisi M, Barbarisi A. From cell–ECM interactions to tissue engineering. Journal of Cellular Physiology. 199(2) (2004) 174-180.
  • 21. Celiz AD, Smith JG, Patel AK, Langer R, Anderson DG, Barrett DA, Young LE, Davies MC, Denning C, Alexander MR. Chemically diverse polymer microarrays and high throughput surface characterisation: a method for discovery of materials for stem cell culture. Biomaterials Science 2(11) (2014) 1604-1611.
  • 22. Sadeghianmaryan A, Karimi Y, Naghieh S, Sardroud HA, Gorji M, Chen X. Electrospinning of scaffolds from the polycaprolactone/polyurethane composite with graphene oxide for skin tissue engineering. Applied Biochemistry and Biotechnology 191 (2020) 567-578.
  • 23. Hejna A, Zedler Ł, Przybysz-Romatowska M, Cañavate J, Colom X, Formela K. Reclaimed rubber/poly (ε-caprolactone) blends: Structure, mechanical, and thermal properties. Polymers 12(5) (2020) 1204.
  • 24. Ansari T, Southgate A, Obiri-Yeboa I, Jones LG, Greco K, Olayanju A, Mbundi L, Somasundaram M, Davidson B, Sibbons PD. Development and Characterization of a Porcine Liver Scaffold. Stem Cells and Development 29(5) (2020) 314-326.
  • 25. Saheli M, Sepantafar M, Pournasr B, Farzaneh Z, Vosough M, Piryaei A, Baharvand H. Three‐dimensional liver‐derived extracellular matrix hydrogel promotes liver organoids function. Journal of Cellular Biochemistry 119(6) (2018) 4320-4333.
  • 26. Srivastava GK, Alonso-Alonso ML, Fernandez-Bueno I, Garcia-Gutierrez MT, Rull F, Medina J, Coco RM, Pastor JC. Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation. Scientific Reports 8(1) (2018) 1-9.
  • 27. Öztürk S, Ayanoğlu FB, Parmaksız M, Elçin AE, Elçin YM. Clinical and surgical aspects of medical materials’ biocompatibility. In: Mozafari M. (eds) Handbook of Biomaterials Biocompatibility. Woodhead Publishing, United Kingdom, pp. 219-250 2020.

Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications

Year 2021, Volume: 8 Issue: 2, 171 - 177, 30.06.2021
https://doi.org/10.17350/HJSE19030000227

Abstract

In this study, nanofibrous biohybrid scaffolds were developed by electrospinning using poly(ε-caprolactone) (PCL) and decellularized bovine tissue derived extracellular matrix (ECM). At the first part of the study, bovine ECM was decellularized by treatment with detergent for 24h and then combined with PCL. Following the evaluation of the decellularization efficiency via spectrophotometric DNA content analysis, the composite scaffolds were characterized by using SEM and FT-IR spectroscopy. Moreover, to assess the biocompatibility of the scaffolds an in vitro cell culture based cytotoxicity test was performed. The results indicated that, DNA content of the bovine tissue was reduced by ~80% compared to the native tissue after decellularization. While FT-IR results indicated the presence of ECM in the composite scaffolds, SEM findings showed that the porous nanofibrous structure of the scaffold changed depending on the incorporated ECM amount. Cell culture based studies also revealed that, the scaffolds containing different amounts of ECM did not have any toxic effect on cell viability during 48 hours of culture period.

References

  • 1. Hoshiba T, Lu H, Kawazoe N, Chen G. Decellularized matrices for tissue engineering. Expert Opinion on Biological Therapy 10(12) (2010) 1717-1728.
  • 2. Lalegül‐Ülker Ö, Vurat MT, Elçin AE, Elçin YM. Magnetic silk fibroin composite nanofibers for biomedical applications: Fabrication and evaluation of the chemical, thermal, mechanical, and in vitro biological properties. Journal of Applied Polymer Science 136(41) (2019) 48040.
  • 3. Vurat MT, Elçin AE, Elçin YM. Osteogenic composite nanocoating based on nanohydroxyapatite, strontium ranelate and polycaprolactone for titanium implants. Transactions of Nonferrous Metals Society of China 28(9) (2018) 1763-1773.
  • 4. Parmaksiz M, Elçin AE, Elçin YM. Decellularized Cell Culture ECMs Act as Cell Differentiation Inducers. Stem Cell Reviews and Reports 16(3) (2020) 569-584.
  • 5. Parmaksiz M, Elçin AE, Elçin YM. Decellularization of bovine small intestinal submucosa and its use for the healing of a critical‐sized full‐thickness skin defect, alone and in combination with stem cells, in a small rodent model. Journal of Tissue Engineering and Regenerative Medicine 11(6) (2017) 1754-1765.
  • 6. Parmaksiz M, Dogan A, Odabas S, Elçin AE, Elçin YM. Clinical applications of decellularized extracellular matrices for tissue engineering and regenerative medicine. Biomedical Materials 11(2) (2016) 022003.
  • 7. Parmaksiz M, Elçin AE, Elçin YM. Decellularized bovine small intestinal submucosa-PCL/hydroxyapatite-based multilayer composite scaffold for hard tissue repair. Materials Science and Engineering C 94 (2019) 788-797
  • 8. Parmaksiz M, Elçin AE, Elçin YM. Decellularization of Bovine Small Intestinal Submucosa. In: Turksen K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, Humana Press, New York, pp. 129-138, 2018.
  • 9. Baptista PM, Siddiqui MM, Lozier G, Rodriguez SR, Atala A, Soker S. The use of whole organ decellularization for the generation of a vascularized liver organoid. Hepatology 53(2) (2011) 604-617.
  • 10. Skardal A., Smith L, Bharadwaj S, Atala A, Soker S, Zhang Y. Tissue specific synthetic ECM hydrogels for 3-D in vitro maintenance of hepatocyte function. Biomaterials 33(18) (2012). 4565–4575.
  • 11. Ren H, Shi X, Tao L, Xiao J, Han B, Zhang Y, Yuan X, Ding Y. Evaluation of two decellularization methods in the development of a whole-organ decellularized rat liver scaffold. Liver International 33(3) (2013) 448-458.
  • 12. Tajima K, Kuroda K, Otaka Y, Kinoshita R, Kita M, Oyamada T, Kanai K. Decellularization of canine kidney for three-dimensional organ regeneration. Veterinary World 13(3) (2020) 452-457.
  • 13. Lewis PL, Yan M, Su J. Shah RN. Directing the growth and alignment of biliary epithelium within extracellular matrix hydrogels. Acta Biomaterialia 85 (2019) 84-93.
  • 14. Mochane MJ, Motsoeneng TS, Sadiku ER, Mokhena TC, Sefadi JS. Morphology and Properties of Electrospun PCL and Its Composites for Medical Applications: A Mini Review. Applied Sciences 9(11) (2019) 2205.
  • 15. Schueren LVD, Schoenmaker BD, Kalaoglu OI, Clerk KD. An alternative solvent system for the steady state electrospinning of polycarbonate. European Polymer Journal 47 (2011) 1256–1263.
  • 16. Luk JZ, Cooper-White J, Rintoul L, Taran E, Grøndahl L. Functionalised polycaprolactone films and 3D scaffolds via gamma irradiation-induced grafting. Journal of Materials Chemistry B 1(33) (2013) 4171-4181.
  • 17. Janarthanan G, Kim IG, Chung EJ, Noh I. Comparative studies on thin polycaprolactone-tricalcium phosphate composite scaffolds and its interaction with mesenchymal stem cells. Biomaterials Research 23(1) (2019) 1-12.
  • 18. Wang X, Ding B, Li B. Biomimetic electrospun nanofibrous structures for tissue engineering. Materials Today 16(6) (2013) 229-241.
  • 19. Moursi AM, Damsky,CH, Lull J, Zimmerman D, Doty SB, Aota S, Globus RK. Fibronectin regulates calvarial osteoblast differentiation. Journal of Cell Science 109 (1996) 1369-1380.
  • 20. Rosso F, Giordano A, Barbarisi M, Barbarisi A. From cell–ECM interactions to tissue engineering. Journal of Cellular Physiology. 199(2) (2004) 174-180.
  • 21. Celiz AD, Smith JG, Patel AK, Langer R, Anderson DG, Barrett DA, Young LE, Davies MC, Denning C, Alexander MR. Chemically diverse polymer microarrays and high throughput surface characterisation: a method for discovery of materials for stem cell culture. Biomaterials Science 2(11) (2014) 1604-1611.
  • 22. Sadeghianmaryan A, Karimi Y, Naghieh S, Sardroud HA, Gorji M, Chen X. Electrospinning of scaffolds from the polycaprolactone/polyurethane composite with graphene oxide for skin tissue engineering. Applied Biochemistry and Biotechnology 191 (2020) 567-578.
  • 23. Hejna A, Zedler Ł, Przybysz-Romatowska M, Cañavate J, Colom X, Formela K. Reclaimed rubber/poly (ε-caprolactone) blends: Structure, mechanical, and thermal properties. Polymers 12(5) (2020) 1204.
  • 24. Ansari T, Southgate A, Obiri-Yeboa I, Jones LG, Greco K, Olayanju A, Mbundi L, Somasundaram M, Davidson B, Sibbons PD. Development and Characterization of a Porcine Liver Scaffold. Stem Cells and Development 29(5) (2020) 314-326.
  • 25. Saheli M, Sepantafar M, Pournasr B, Farzaneh Z, Vosough M, Piryaei A, Baharvand H. Three‐dimensional liver‐derived extracellular matrix hydrogel promotes liver organoids function. Journal of Cellular Biochemistry 119(6) (2018) 4320-4333.
  • 26. Srivastava GK, Alonso-Alonso ML, Fernandez-Bueno I, Garcia-Gutierrez MT, Rull F, Medina J, Coco RM, Pastor JC. Comparison between direct contact and extract exposure methods for PFO cytotoxicity evaluation. Scientific Reports 8(1) (2018) 1-9.
  • 27. Öztürk S, Ayanoğlu FB, Parmaksız M, Elçin AE, Elçin YM. Clinical and surgical aspects of medical materials’ biocompatibility. In: Mozafari M. (eds) Handbook of Biomaterials Biocompatibility. Woodhead Publishing, United Kingdom, pp. 219-250 2020.
There are 27 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Research Article
Authors

Mahmut PARMAKSIZ 0000-0002-4655-1401

Publication Date June 30, 2021
Submission Date March 11, 2021
Published in Issue Year 2021 Volume: 8 Issue: 2

Cite

Vancouver PARMAKSIZ M. Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications. Hittite J Sci Eng. 2021;8(2):171-7.

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