Year 2021, Volume 8 , Issue 2, Pages 171 - 177 2021-06-30

Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications

Mahmut PARMAKSIZ [1]


In this study, nanofibrous biohybrid scaffolds were developed by electrospinning using poly(ε-caprolactone) (PCL) and decellularized bovine tissue derived extracellular matrix (ECM). At the first part of the study, bovine ECM was decellularized by treatment with detergent for 24h and then combined with PCL. Following the evaluation of the decellularization efficiency via spectrophotometric DNA content analysis, the composite scaffolds were characterized by using SEM and FT-IR spectroscopy. Moreover, to assess the biocompatibility of the scaffolds an in vitro cell culture based cytotoxicity test was performed. The results indicated that, DNA content of the bovine tissue was reduced by ~80% compared to the native tissue after decellularization. While FT-IR results indicated the presence of ECM in the composite scaffolds, SEM findings showed that the porous nanofibrous structure of the scaffold changed depending on the incorporated ECM amount. Cell culture based studies also revealed that, the scaffolds containing different amounts of ECM did not have any toxic effect on cell viability during 48 hours of culture period.
biomaterials, tissue engineering, decellularization, electrospinning
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Primary Language en
Subjects Engineering
Journal Section Research Article
Authors

Orcid: 0000-0002-4655-1401
Author: Mahmut PARMAKSIZ (Primary Author)
Institution: ANKARA UNIVERSITY
Country: Turkey


Dates

Application Date : March 11, 2021
Acceptance Date : June 10, 2021
Publication Date : June 30, 2021

Bibtex @research article { hjse894274, journal = {Hittite Journal of Science and Engineering}, issn = {}, eissn = {2148-4171}, address = {Hitit Üniversitesi Mühendislik Fakültesi Kuzey Kampüsü Çevre Yolu Bulvarı 19030 Çorum / TÜRKİYE}, publisher = {Hitit University}, year = {2021}, volume = {8}, pages = {171 - 177}, doi = {10.17350/HJSE19030000227}, title = {Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications}, key = {cite}, author = {Parmaksız, Mahmut} }
APA Parmaksız, M . (2021). Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications . Hittite Journal of Science and Engineering , 8 (2) , 171-177 . DOI: 10.17350/HJSE19030000227
MLA Parmaksız, M . "Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications" . Hittite Journal of Science and Engineering 8 (2021 ): 171-177 <https://dergipark.org.tr/en/pub/hjse/issue/63382/894274>
Chicago Parmaksız, M . "Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications". Hittite Journal of Science and Engineering 8 (2021 ): 171-177
RIS TY - JOUR T1 - Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications AU - Mahmut Parmaksız Y1 - 2021 PY - 2021 N1 - doi: 10.17350/HJSE19030000227 DO - 10.17350/HJSE19030000227 T2 - Hittite Journal of Science and Engineering JF - Journal JO - JOR SP - 171 EP - 177 VL - 8 IS - 2 SN - -2148-4171 M3 - doi: 10.17350/HJSE19030000227 UR - https://doi.org/10.17350/HJSE19030000227 Y2 - 2021 ER -
EndNote %0 Hittite Journal of Science and Engineering Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications %A Mahmut Parmaksız %T Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications %D 2021 %J Hittite Journal of Science and Engineering %P -2148-4171 %V 8 %N 2 %R doi: 10.17350/HJSE19030000227 %U 10.17350/HJSE19030000227
ISNAD Parmaksız, Mahmut . "Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications". Hittite Journal of Science and Engineering 8 / 2 (June 2021): 171-177 . https://doi.org/10.17350/HJSE19030000227
AMA Parmaksız M . Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications. Hittite J Sci Eng. 2021; 8(2): 171-177.
Vancouver Parmaksız M . Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications. Hittite Journal of Science and Engineering. 2021; 8(2): 171-177.
IEEE M. Parmaksız , "Development of a Nanofibrous Scaffold Based on Bovine Tissue-derived ECM and Poly(ε-caprolactone) for Tissue Engineering Applications", Hittite Journal of Science and Engineering, vol. 8, no. 2, pp. 171-177, Jun. 2021, doi:10.17350/HJSE19030000227