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Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients

Year 2021, Volume 8, Issue 3, 267 - 272, 29.09.2021
https://doi.org/10.17350/HJSE19030000238

Abstract

Myrtus communis L. (Myrtaceae) is a plant widely found in the Middle East and Mediterranean region, both naturally grown and cultivated. In our study, C. elegans nematodes were used as an anti-helminthic model and by determining the anti-helmintic effect of Myrtus communis L. fruit, it was aimed to find the active ingredients with possible anti-helmintic effects by GC-MS analysis. In our study, the anthelmintic effect of the ethanol extract of Myrtus communis L. fruit on C. elegans was investigated. Ethanol fruit extracts were added to NGM separately at final volume of 10 mg/mL, 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL and experimental groups were formed. When the data were evaluated statistically, the difference between all experimental groups, negative control group and positive control group (pyrantel pamoate), was significant (p<0.05). According to the results of GC-MS analysis, the highest detected ingredients were 1,8-Cineole (3.46%), Linalyl acetate (3.06%), and Palmitic acid (2.90%). It was observed that Myrtus communis L. fruit extract was more effective on C. elegans nematodes than pyrantel pamoate at the same concentration (5 mg/mL), and 20 different active ingredients were determined according to our GC-MS analysis results. By studying the forms of these active ingredients separately or in different combinations, the ingredients and combinations with the strongest anti-helmintic activity can be determined, and it is thought that our findings may lead to further studies.

References

  • 1. Traveset A, Riera N, Mas RE. Ecology of fruit-colour polymorphism in Myrtus communis and differential effects of birds and mammals on seed germination and seedling growth. Journal of Ecology (2001) 749-760.
  • 2. Morel J-P, Mercuri AM. Plants and Culture: seeds of the cultural heritage of Europe: Centro Europeo per. I Beni Culturali Ravello Edipuglia (2009) 1-284.
  • 3. Baytop T. Therapy with medicinal plants in Turkey (Past and Present). Publication of the Istanbul University (1999) 312.
  • 4. Aleksic V, Knezevic P. Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L. Microbiological research 169 (2014) 240-254.
  • 5. Gortzi O, Lalas S, Chinou I, Tsaknis J. Reevaluation of bioactivity and antioxidant activity of Myrtus communis extract before and after encapsulation in liposomes. European food research and technology 226 (2008) 583-590.
  • 6. El Hartiti H, El Mostaphi A, Barrahi M, Ben Ali A, Chahboun N. Chemical composition and antibacterial activity of the essential oil of Myrtus communis leaves. Karbala International Journal of Modern Science 6 (2020) 249-258.
  • 7. Sadeghi Nejad B, Erfani Nejad M, Yusef Naanaie S, Zarrin M. Antifungal efficacy of Myrtus communis Linn. Jentashapir Journal of Health Research 5 (2014) e21879.
  • 8. Tuberoso CIG., Rosa, A., Bifulco E, Melis MP, Atzeri A, Pirisi FM, & Dessì MA. Chemical composition and antioxidant activities of Myrtus communis L. berries extracts. Food Chemistry 123 (2010) 1242-1251.
  • 9. Gul C, Nazligul Y. A Current Approach to Helminthiasis. Dirim Medical Journal 83 (2008) 40-48.
  • 10. Bethony J, Brooker S, Albonico M, Geiger, SM, Loukas A, Diemert D, & Hotez PJ. Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm. The Lancet 367 (2006) 1521-1532.
  • 11. Drake L, Bundy D. Multiple helminth infections in children: impact and control. Parasitology 122 (2001) 73-81.
  • 12. Gatti S, Lopes R, Cevini C, Ijaoba, B, Bruno A, Bernuzzi AM, Scaglia M. Intestinal parasitic infections in an institution for the mentally retarded. Annals of Tropical Medicine & Parasitology 94 (2000) 453-460.
  • 13. Tappeh KH, Mohammadzadeh H, Rahim RN, Barazesh A, Khashaveh S, Taherkhani H. Prevalence of intestinal parasitic infections among mentally disabled children and adults of Urmia, Iran. Iranian journal of parasitology 5 (2010) 60-64.
  • 14. Kaminsky R, Ducray P, Jung M, Clover R, Rufener L, Bouvier J & Mäser P. A new class of anthelmintics effective against drug-resistant nematodes. Nature 452 (2008) 176-181.
  • 15. Smout MJ, Kotze AC, McCarthy JS, Loukas A. A novel high throughput assay for anthelmintic drug screening and resistance diagnosis by real-time monitoring of parasite motility. PloS neglected tropical diseases 4 (2010) e885.
  • 16. Ozpınar N. A model Organism for Antihelminthic Activity: Caenorhabditis elegans and Nigella sativa. Turkish Journal of Parasitology 44 (2020) 31.
  • 17. Hernando G, Turani O, Bouzat C. Caenorhabditis elegans muscle Cys-loop receptors as novel targets of terpenoids with potential anthelmintic activity. Plos Neglected Tropical Diseases 13 (2019) e0007895.
  • 18. Kaewintajuk K, Cho PY, Kim SY, Lee ES, Lee HK, Choi EB, & Park, H. Anthelmintic activity of KSI-4088 against Caenorhabditis elegans. Parasitology research 107 (2010) 27-30.
  • 19. Shaapan RM, Al-Abodi HR, Alanazi AD, Abdel-Shafy S, Rashidipour M, Shater AF, & Mahmoudvand H. Myrtus communis Essential Oil; Anti-Parasitic Effects and Induction of the Innate Immune System in Mice with Toxoplasma gondii Infection. Molecules 26 (2021) 819.
  • 20. Mahmoudvand H, Ezzatkhah F, Sharififar F, Sharifi I, Dezaki ES. Antileishmanial and cytotoxic effects of essential oil and methanolic extract of Myrtus communis L. The Korean Journal of Parasitology 53 (2015) 21-27.
  • 21. Abdalla SF, Ramadan NI, Mohamed AA, El-Deeb HK, Al-Khadrawy FM, Badawy AF. A study on the effect of Myrtus communis and Olibanum on Giardia lamblia infection in Egypt. Parasitologists United Journal 4 (2011) 89-100.
  • 22. Azadbakht M, Ziaiye H, Abdollahi F, Shabankhani B. Effect of Methanolic essence and extract of Myrtus Communis on Trichomonas Vaginalis. Journal of Guilan University of Medical Sciences 12 (2004) 8-13.
  • 23. Abdollahy F, Ziaei H, Shabankhani B, Azadbakht M. Effect of essential oils of Artemisia aucheri Boiss. Zataria multiflora Boiss, and Myrtus communis L. on Trichomonas vaginalis. Iranian Journal of Pharmaceutical Research (2010) 35.
  • 24. Moussouni L, Besseboua O, Abdelhanine A. Anthelmintic activity of aqueous and ethanol extracts of Urtica dioica L. and Myrtus communis L. leaves on bovine digestive strongyles: in-vitro study. Ataturk University Journal of Veterinary Sciences 14 (2019) 273-283.
  • 25. Vickers NJ. Animal communication: when I’m calling you, will you answer too? Current Biology 27 (2017) 713-715.
  • 26. Oliveira GL, Vieira TM, Nunes VF, Ruas MDO, Duarte ER, Moreira DDL, & Martins ER. Chemical composition and efficacy in the egg-hatching inhibition of essential oil of Piper aduncum against Haemonchus contortus from sheep. Revista Brasileira de Farmacognosia 24 (2014) 288-292.
  • 27. Pinto, N. B., Castro, L. M. D., Azambuja, R. H. M., Capella, G. D. A., Moura, M. Q. D., Terto, W. D., ... & Leite, F. P. L. (2019). Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep. Revista Brasileira de Parasitologia Veterinária, 28, 807-811.
  • 28. Pessoa L, Morais S, Bevilaqua C, Luciano J. Anthelmintic activity of essential oil of Ocimum gratissimum Linn. and eugenol against Haemonchus contortus. Veterinary Parasitology 109 (2002) 59-63.
  • 29. Malheiros DF, Sarquis IR, Ferreira IM, Mathews PD, Mertins O, Tavares‐Dias M. Nanoemulsions with oleoresin of Copaifera reticulata (Leguminosae) improve anthelmintic efficacy in the control of monogenean parasites when compared to oleoresin without nanoformulation. Journal of Fish Diseases 43 (2020) 687-695.
  • 30. Lalthanpuii PB, Lalchhandama K. Phytochemical analysis and in vitro anthelmintic activity of Imperata cylindrica underground parts. BMC Complementary Medicine and Therapies 20 (2020) 1-9.
  • 31. Lalthanpuii P, Zokimi Z, Lalchhandama K. The toothache plant (Acmella oleracea) exhibits anthelmintic activity on both parasitic tapeworms and roundworms. Pharmacognosy Magazine 16 (2020) 193-198.
  • 32. Hernandez PM, Salem AZ, Elghandour MM, Cipriano-Salazar M, Cruz-Lagunas B, Camacho LM. Anthelmintic effects of Salix babylonica L. and Leucaena leucocephala Lam. extracts in growing lambs. Tropical Animal Health and Production 46 (2014) 173-178.
  • 33. Panda SK, Das R, Mai AH, De Borggraeve WM, Luyten W. Nematicidal Activity of Holigarna caustica (Dennst.) Oken Fruit Is Due to Linoleic Acid. Biomolecules 10 (2020) 1043-1054.
  • 34. Štrbac F, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. In vitro ovicidal activity of two chemotypes of the yarrow (Achillea millefolium L.) essential oil against sheep gastrointestinal nematodes. Archives of Veterinary Medicine 13 (2020) 59-76.
  • 35. Taur D, Kulkarni V, Patil R. Chromatographic evaluation and anthelmintic activity of Eucalyptus globulus oil. Pharmacognosy Research 2 (2010) 125-127.
  • 36. Kaplan RM, Storey BE, Vidyashankar AN, Bissinger BW, Mitchell SM, Howell SB, Mason ME, Lee MD, Pedroso AA, Akashe A, Skrypec DJ. Antiparasitic efficacy of a novel plant-based functional food using an Ascaris suum model in pigs. Acta Tropica 139 (2014) 15-22.
  • 37. Matos-Rocha TJ, dos Santos Cavalcanti MG, Barbosa-Filho JM, et al. In vitro evaluation of schistosomicidal activity of essential oil of Mentha x villosa and some of its chemical constituents in adult worms of Schistosoma mansoni. Planta Medica. 79 (2013) 1307-1312.

Year 2021, Volume 8, Issue 3, 267 - 272, 29.09.2021
https://doi.org/10.17350/HJSE19030000238

Abstract

References

  • 1. Traveset A, Riera N, Mas RE. Ecology of fruit-colour polymorphism in Myrtus communis and differential effects of birds and mammals on seed germination and seedling growth. Journal of Ecology (2001) 749-760.
  • 2. Morel J-P, Mercuri AM. Plants and Culture: seeds of the cultural heritage of Europe: Centro Europeo per. I Beni Culturali Ravello Edipuglia (2009) 1-284.
  • 3. Baytop T. Therapy with medicinal plants in Turkey (Past and Present). Publication of the Istanbul University (1999) 312.
  • 4. Aleksic V, Knezevic P. Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L. Microbiological research 169 (2014) 240-254.
  • 5. Gortzi O, Lalas S, Chinou I, Tsaknis J. Reevaluation of bioactivity and antioxidant activity of Myrtus communis extract before and after encapsulation in liposomes. European food research and technology 226 (2008) 583-590.
  • 6. El Hartiti H, El Mostaphi A, Barrahi M, Ben Ali A, Chahboun N. Chemical composition and antibacterial activity of the essential oil of Myrtus communis leaves. Karbala International Journal of Modern Science 6 (2020) 249-258.
  • 7. Sadeghi Nejad B, Erfani Nejad M, Yusef Naanaie S, Zarrin M. Antifungal efficacy of Myrtus communis Linn. Jentashapir Journal of Health Research 5 (2014) e21879.
  • 8. Tuberoso CIG., Rosa, A., Bifulco E, Melis MP, Atzeri A, Pirisi FM, & Dessì MA. Chemical composition and antioxidant activities of Myrtus communis L. berries extracts. Food Chemistry 123 (2010) 1242-1251.
  • 9. Gul C, Nazligul Y. A Current Approach to Helminthiasis. Dirim Medical Journal 83 (2008) 40-48.
  • 10. Bethony J, Brooker S, Albonico M, Geiger, SM, Loukas A, Diemert D, & Hotez PJ. Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm. The Lancet 367 (2006) 1521-1532.
  • 11. Drake L, Bundy D. Multiple helminth infections in children: impact and control. Parasitology 122 (2001) 73-81.
  • 12. Gatti S, Lopes R, Cevini C, Ijaoba, B, Bruno A, Bernuzzi AM, Scaglia M. Intestinal parasitic infections in an institution for the mentally retarded. Annals of Tropical Medicine & Parasitology 94 (2000) 453-460.
  • 13. Tappeh KH, Mohammadzadeh H, Rahim RN, Barazesh A, Khashaveh S, Taherkhani H. Prevalence of intestinal parasitic infections among mentally disabled children and adults of Urmia, Iran. Iranian journal of parasitology 5 (2010) 60-64.
  • 14. Kaminsky R, Ducray P, Jung M, Clover R, Rufener L, Bouvier J & Mäser P. A new class of anthelmintics effective against drug-resistant nematodes. Nature 452 (2008) 176-181.
  • 15. Smout MJ, Kotze AC, McCarthy JS, Loukas A. A novel high throughput assay for anthelmintic drug screening and resistance diagnosis by real-time monitoring of parasite motility. PloS neglected tropical diseases 4 (2010) e885.
  • 16. Ozpınar N. A model Organism for Antihelminthic Activity: Caenorhabditis elegans and Nigella sativa. Turkish Journal of Parasitology 44 (2020) 31.
  • 17. Hernando G, Turani O, Bouzat C. Caenorhabditis elegans muscle Cys-loop receptors as novel targets of terpenoids with potential anthelmintic activity. Plos Neglected Tropical Diseases 13 (2019) e0007895.
  • 18. Kaewintajuk K, Cho PY, Kim SY, Lee ES, Lee HK, Choi EB, & Park, H. Anthelmintic activity of KSI-4088 against Caenorhabditis elegans. Parasitology research 107 (2010) 27-30.
  • 19. Shaapan RM, Al-Abodi HR, Alanazi AD, Abdel-Shafy S, Rashidipour M, Shater AF, & Mahmoudvand H. Myrtus communis Essential Oil; Anti-Parasitic Effects and Induction of the Innate Immune System in Mice with Toxoplasma gondii Infection. Molecules 26 (2021) 819.
  • 20. Mahmoudvand H, Ezzatkhah F, Sharififar F, Sharifi I, Dezaki ES. Antileishmanial and cytotoxic effects of essential oil and methanolic extract of Myrtus communis L. The Korean Journal of Parasitology 53 (2015) 21-27.
  • 21. Abdalla SF, Ramadan NI, Mohamed AA, El-Deeb HK, Al-Khadrawy FM, Badawy AF. A study on the effect of Myrtus communis and Olibanum on Giardia lamblia infection in Egypt. Parasitologists United Journal 4 (2011) 89-100.
  • 22. Azadbakht M, Ziaiye H, Abdollahi F, Shabankhani B. Effect of Methanolic essence and extract of Myrtus Communis on Trichomonas Vaginalis. Journal of Guilan University of Medical Sciences 12 (2004) 8-13.
  • 23. Abdollahy F, Ziaei H, Shabankhani B, Azadbakht M. Effect of essential oils of Artemisia aucheri Boiss. Zataria multiflora Boiss, and Myrtus communis L. on Trichomonas vaginalis. Iranian Journal of Pharmaceutical Research (2010) 35.
  • 24. Moussouni L, Besseboua O, Abdelhanine A. Anthelmintic activity of aqueous and ethanol extracts of Urtica dioica L. and Myrtus communis L. leaves on bovine digestive strongyles: in-vitro study. Ataturk University Journal of Veterinary Sciences 14 (2019) 273-283.
  • 25. Vickers NJ. Animal communication: when I’m calling you, will you answer too? Current Biology 27 (2017) 713-715.
  • 26. Oliveira GL, Vieira TM, Nunes VF, Ruas MDO, Duarte ER, Moreira DDL, & Martins ER. Chemical composition and efficacy in the egg-hatching inhibition of essential oil of Piper aduncum against Haemonchus contortus from sheep. Revista Brasileira de Farmacognosia 24 (2014) 288-292.
  • 27. Pinto, N. B., Castro, L. M. D., Azambuja, R. H. M., Capella, G. D. A., Moura, M. Q. D., Terto, W. D., ... & Leite, F. P. L. (2019). Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep. Revista Brasileira de Parasitologia Veterinária, 28, 807-811.
  • 28. Pessoa L, Morais S, Bevilaqua C, Luciano J. Anthelmintic activity of essential oil of Ocimum gratissimum Linn. and eugenol against Haemonchus contortus. Veterinary Parasitology 109 (2002) 59-63.
  • 29. Malheiros DF, Sarquis IR, Ferreira IM, Mathews PD, Mertins O, Tavares‐Dias M. Nanoemulsions with oleoresin of Copaifera reticulata (Leguminosae) improve anthelmintic efficacy in the control of monogenean parasites when compared to oleoresin without nanoformulation. Journal of Fish Diseases 43 (2020) 687-695.
  • 30. Lalthanpuii PB, Lalchhandama K. Phytochemical analysis and in vitro anthelmintic activity of Imperata cylindrica underground parts. BMC Complementary Medicine and Therapies 20 (2020) 1-9.
  • 31. Lalthanpuii P, Zokimi Z, Lalchhandama K. The toothache plant (Acmella oleracea) exhibits anthelmintic activity on both parasitic tapeworms and roundworms. Pharmacognosy Magazine 16 (2020) 193-198.
  • 32. Hernandez PM, Salem AZ, Elghandour MM, Cipriano-Salazar M, Cruz-Lagunas B, Camacho LM. Anthelmintic effects of Salix babylonica L. and Leucaena leucocephala Lam. extracts in growing lambs. Tropical Animal Health and Production 46 (2014) 173-178.
  • 33. Panda SK, Das R, Mai AH, De Borggraeve WM, Luyten W. Nematicidal Activity of Holigarna caustica (Dennst.) Oken Fruit Is Due to Linoleic Acid. Biomolecules 10 (2020) 1043-1054.
  • 34. Štrbac F, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. In vitro ovicidal activity of two chemotypes of the yarrow (Achillea millefolium L.) essential oil against sheep gastrointestinal nematodes. Archives of Veterinary Medicine 13 (2020) 59-76.
  • 35. Taur D, Kulkarni V, Patil R. Chromatographic evaluation and anthelmintic activity of Eucalyptus globulus oil. Pharmacognosy Research 2 (2010) 125-127.
  • 36. Kaplan RM, Storey BE, Vidyashankar AN, Bissinger BW, Mitchell SM, Howell SB, Mason ME, Lee MD, Pedroso AA, Akashe A, Skrypec DJ. Antiparasitic efficacy of a novel plant-based functional food using an Ascaris suum model in pigs. Acta Tropica 139 (2014) 15-22.
  • 37. Matos-Rocha TJ, dos Santos Cavalcanti MG, Barbosa-Filho JM, et al. In vitro evaluation of schistosomicidal activity of essential oil of Mentha x villosa and some of its chemical constituents in adult worms of Schistosoma mansoni. Planta Medica. 79 (2013) 1307-1312.

Details

Primary Language English
Subjects Basic Sciences
Journal Section Research Articles
Authors

Hülya ÖZPINAR (Primary Author)
SIVAS CUMHURIYET UNIVERSITY
0000-0001-8154-0874
Türkiye

Publication Date September 29, 2021
Application Date August 17, 2021
Acceptance Date August 26, 2021
Published in Issue Year 2021, Volume 8, Issue 3

Cite

Bibtex @research article { hjse984044, journal = {Hittite Journal of Science and Engineering}, issn = {}, eissn = {2148-4171}, address = {Hitit Üniversitesi Mühendislik Fakültesi Kuzey Kampüsü Çevre Yolu Bulvarı 19030 Çorum / TÜRKİYE}, publisher = {Hitit University}, year = {2021}, volume = {8}, pages = {267 - 272}, doi = {10.17350/HJSE19030000238}, title = {Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients}, key = {cite}, author = {Özpınar, Hülya} }
APA Özpınar, H. (2021). Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients . Hittite Journal of Science and Engineering , 8 (3) , 267-272 . DOI: 10.17350/HJSE19030000238
MLA Özpınar, H. "Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients" . Hittite Journal of Science and Engineering 8 (2021 ): 267-272 <https://dergipark.org.tr/en/pub/hjse/issue/65166/984044>
Chicago Özpınar, H. "Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients". Hittite Journal of Science and Engineering 8 (2021 ): 267-272
RIS TY - JOUR T1 - Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients AU - Hülya Özpınar Y1 - 2021 PY - 2021 N1 - doi: 10.17350/HJSE19030000238 DO - 10.17350/HJSE19030000238 T2 - Hittite Journal of Science and Engineering JF - Journal JO - JOR SP - 267 EP - 272 VL - 8 IS - 3 SN - -2148-4171 M3 - doi: 10.17350/HJSE19030000238 UR - https://doi.org/10.17350/HJSE19030000238 Y2 - 2021 ER -
EndNote %0 Hittite Journal of Science and Engineering Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients %A Hülya Özpınar %T Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients %D 2021 %J Hittite Journal of Science and Engineering %P -2148-4171 %V 8 %N 3 %R doi: 10.17350/HJSE19030000238 %U 10.17350/HJSE19030000238
ISNAD Özpınar, Hülya . "Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients". Hittite Journal of Science and Engineering 8 / 3 (September 2021): 267-272 . https://doi.org/10.17350/HJSE19030000238
AMA Özpınar H. Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients. Hittite J Sci Eng. 2021; 8(3): 267-272.
Vancouver Özpınar H. Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients. Hittite Journal of Science and Engineering. 2021; 8(3): 267-272.
IEEE H. Özpınar , "Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients", Hittite Journal of Science and Engineering, vol. 8, no. 3, pp. 267-272, Sep. 2021, doi:10.17350/HJSE19030000238