Research Article
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Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats

Year 2021, Volume 8, Issue 4, 299 - 305, 31.12.2021
https://doi.org/10.17350/HJSE19030000242

Abstract

Wound healing is a complex and dynamic process that includes multiple biological pathways and has some successive healing periods. Most growth factor is responsible for wound healing. Fibroblast growth factor has a positive effect on wound healing problems that can be caused by diabetes. In the present study, we aimed to investigate exogenous effect bFGF supplementation on serum TBARS, RSH and NOx levels in hyperglysemic rats. Experiments were performed on 30 male Wistar albino rats (weight range: 200-250 g ). Rats were hyperglycemic with STZ (ip, 60 mg/kg). The experimental groups were divided into untreated and bFGF-treated subgroups. bGF was applied locally to the dorsalateral wounds of rats (10 ng/ml). After these administrations, on the 3th and 7th days of wound healing, the animals were sacrificed. Serum TBARS, RSH and NOx levels were recorded spectrophotometrically. The results were expressed as mean ± Standard deviation and the mean differences were compared by Anova Variance Analysis (p<0,05). When compared with the treatment group, on the 7th day and the 3rd day, it was found that the serum TBARS levels increased statistically in hyperglycemic rats(p<0,05). Both in the 3rd day of the untreated and 3rd day of the rats treated with bFGF may significant decrease in the serum RSH levels. bFGF application was found both enhancing and reducing effects on oxidative stres. In subsequent studies, the effect of bFGF, which has positive effects on diabetic wound healing, on oxidative events can be investigated in detail using different doses and different treatment periods.

References

  • Reference1. Yüceyar, S. (2008). Cerrahi Yaraların Takip ve Tedavisi, İ. Ü. Cerrahpaşa Tıp Fakültesi Sürekli Tıp Eğitimi Etkinlikleri Sempozyum Dizisi, İstanbul. 67, 17-24.
  • Reference2. Gerald, M.C., Diane, R.K., David, J.M., David, E.P., Roger, R. and Martin, C. (1994). Definitions and guidelines for assessment of wounds and evaluation of healing. Wound Repair and Regeneration, 2(2), 165–170.
  • Reference3. Barrientos, S., Brem, H., Canic, M.C., Golinko., M.S. and Stojadinovic, O. (2008). Growth factors and cytokines in wound healing. Wound Repair and Regeneration, 16(5), 585–601.
  • Reference4. Karasu, A., Bakır, B. (2008). Yara ve Yara İyileşmesi. Veteriner Cerrahi Dergisi, 14(1), 36-43.
  • Reference5. Taşır, S. (2007). Ekzojen epidermal büyüme faktörü’nün tükürük bezi oksidan olaylarına zaman bağımlı etkisi, Yüksek Lisans Tezi, Gazi Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
  • Reference6. Takayama, Y. and Aoki, R. (2012). Roles of lactoferrin on skin wound healing. Biochemistry and Cell Biology, 90(3), 497-503. Reference7. Anusakthien, O. and Giannobile, W.V. (2002) Growth factor delivery to reengineer periodontal tissues. Current Pharmaceutical Biotechnology, 3, 129-139.
  • Reference8. Jin Lee, S. (2000). Cytokine delivery and tissue engineering. Yonsei Medical Journal, 41(6), 704-719.
  • Reference9. Ornitz, D. M., Itoh, N. (2001). Fibroblast growth factors. Genome Biology, 2(3), 3005-1.
  • Reference10. Casey-Sawicki, K., Zhang, M., Kim, S., Zhang, A., Zhang, S., Swarts, S., Vidyasagar, S., Zhang, L., Zhang, A., Okunieff, P. (2014). A Basic Fibroblast Growth Factor Analog for Protection and Mitigation against Acute Radiation Syndromes. Health Physics, 106(6), 704-712.
  • Reference11. Teven, C. M., Farina, E. M., Rivas, J., Reid, R. R. (2014). Fibroblast growth factor (FGF) signaling in development and skeletal diseases. Genes & Diseases, 1(2), 199–213.
  • Reference12. Yıldırım, A., Tunik, S., Çetin, Ç. ve Akkuş, M. (2009). Osteogenezde Fibroblast Büyüme Faktörleri (FGF) ve Kemik Morfogenetik Proteinlerin (Kmp) rolü. İnönü Üniversitesi Tıp Fakültesi Dergisi, 16(2), 135-140.
  • Reference13. Kobayashi, F., Matsuzaka, K. and Inoue, T. (2015). The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands. International Journal of Oral Science, 8(1), 16-23.
  • Reference14. Özler, M., Şimşek, K., Topal, T., Öter, Ş. ve Korkmaz, A. (2010). Pinealektomili Ratlarda Yara İyileşmesi. Gülhane Tıp Dergisi, 52, 181-184. Reference15. Luft, R. and Minkowski, O. (1989). Discovery of the pancreatic origin of diyabetes. Diyabetologia, 32(7), 399-401.
  • Reference16. Baynes, J. W., Thorpe, S. R. (1999). Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. Diabetes, 48(1), 1–9.
  • Reference17. Dandona, P., Thusu, K., Cook, S., Snyder, B., Makowski, J., Armstrong, D. and Nicotera, T. (1996). Oxidative damage to DNA in diabetes mellitus. The Lancet, 347(8999), 444-445.
  • Reference18. Aslan, D., Onat, T., Emerk, K. ve Sözmen, E. Y., (2006). İnsan Biyokimyası (İkinci Baskı), Ankara: Palme Yayıncılık. 691-699, 714.
  • Reference19. Pitocco, D., Zaccardi, F., Di Stasio, E., Romitelli, F., Santini, S. A., Zuppi, C., Ghirlanda, G. (2010). Oxidative stress, nitric oxide and diabetes. The review of Diabetic Studies, 7(1), 15-25
  • Reference20. Kurtel, H., Granger, D. N., Tso, P., Grisham, M. B. (1992). Vulnerability of intestinal interstitial fluid to oxidant stress. American Journal of Physiology-Gastrointestinal and Liver Physiology, 263(4), 573-578.
  • Reference21. Miranda, K. M., Espey, M. G., Wink, D. A. (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric oxide, 5(1), 62-71.
  • Reference22. Aydemir, B., Sarı, E.K. (2009). Antioksidanlar ve Büyüme Faktörleri ile İlişkisi, Kocatepe Veteriner Dergisi, 2(2), 56-60.
  • Reference23. Altan, N., Dinçel, A. S., and Koca, C. (2006). Diabetes Mellitus ve Oksidatif Stres, Türkiye Biyokimya Dergisi., 31(2), 51-56.
  • Reference24. Çetin, M. ve Çapan, Y. (2004). Bazik Fibroblast Büyüme Faktörü ve Formülasyonlarında Yeni Yaklaşımlar, Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, 24(2), 107-124.
  • Reference25. Yazıcı, A. E., Paşaoğlu, H., Yücel, D., Çelebi, N., Bakır, F., Özkaya, M. (2002). Tip II Diabetes Mellituslu Hastalarda Plazma Total Tiyol ve Eritrosit Redükte Glutatyon Düzeyleri. Turkiye Klinikleri Journal of Medical Sciences, 22(5), 487-492.
  • Reference26. Hu, M. L. (1994). Measurement of protein thiol groups and glutathione in plasma. In Methods in enzymology. 233, 380-385
  • Reference27. Karabağ, F. (2010). Deneysel Olarak Hipertiroidizm Olusturulan Ratlarda Kafeik Asit Fenetil Ester’in Plazma Homosistein, Asimetrik Dimetil Arjinin, Nitrik Oksit ve Lipid Profili Üzerine Etkilerinin Araştırılması, Doktora Tezi, Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Afyonkarahisar, 16-17.
  • Reference28. Chin, G. A., Diegelmann, R. F., Schultz, G. S. (2005). Cellular and molecular regulation of wound healing. Basic and Clinical Dermatology, 17-37.
  • Reference29. Memişoğulları, R., (2006). Diyabette serbest radikallerin rolü ve antioksidanların etkisi, Düzce Tıp Fakültesi Dergisi, 3, 30-39.

Year 2021, Volume 8, Issue 4, 299 - 305, 31.12.2021
https://doi.org/10.17350/HJSE19030000242

Abstract

References

  • Reference1. Yüceyar, S. (2008). Cerrahi Yaraların Takip ve Tedavisi, İ. Ü. Cerrahpaşa Tıp Fakültesi Sürekli Tıp Eğitimi Etkinlikleri Sempozyum Dizisi, İstanbul. 67, 17-24.
  • Reference2. Gerald, M.C., Diane, R.K., David, J.M., David, E.P., Roger, R. and Martin, C. (1994). Definitions and guidelines for assessment of wounds and evaluation of healing. Wound Repair and Regeneration, 2(2), 165–170.
  • Reference3. Barrientos, S., Brem, H., Canic, M.C., Golinko., M.S. and Stojadinovic, O. (2008). Growth factors and cytokines in wound healing. Wound Repair and Regeneration, 16(5), 585–601.
  • Reference4. Karasu, A., Bakır, B. (2008). Yara ve Yara İyileşmesi. Veteriner Cerrahi Dergisi, 14(1), 36-43.
  • Reference5. Taşır, S. (2007). Ekzojen epidermal büyüme faktörü’nün tükürük bezi oksidan olaylarına zaman bağımlı etkisi, Yüksek Lisans Tezi, Gazi Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
  • Reference6. Takayama, Y. and Aoki, R. (2012). Roles of lactoferrin on skin wound healing. Biochemistry and Cell Biology, 90(3), 497-503. Reference7. Anusakthien, O. and Giannobile, W.V. (2002) Growth factor delivery to reengineer periodontal tissues. Current Pharmaceutical Biotechnology, 3, 129-139.
  • Reference8. Jin Lee, S. (2000). Cytokine delivery and tissue engineering. Yonsei Medical Journal, 41(6), 704-719.
  • Reference9. Ornitz, D. M., Itoh, N. (2001). Fibroblast growth factors. Genome Biology, 2(3), 3005-1.
  • Reference10. Casey-Sawicki, K., Zhang, M., Kim, S., Zhang, A., Zhang, S., Swarts, S., Vidyasagar, S., Zhang, L., Zhang, A., Okunieff, P. (2014). A Basic Fibroblast Growth Factor Analog for Protection and Mitigation against Acute Radiation Syndromes. Health Physics, 106(6), 704-712.
  • Reference11. Teven, C. M., Farina, E. M., Rivas, J., Reid, R. R. (2014). Fibroblast growth factor (FGF) signaling in development and skeletal diseases. Genes & Diseases, 1(2), 199–213.
  • Reference12. Yıldırım, A., Tunik, S., Çetin, Ç. ve Akkuş, M. (2009). Osteogenezde Fibroblast Büyüme Faktörleri (FGF) ve Kemik Morfogenetik Proteinlerin (Kmp) rolü. İnönü Üniversitesi Tıp Fakültesi Dergisi, 16(2), 135-140.
  • Reference13. Kobayashi, F., Matsuzaka, K. and Inoue, T. (2015). The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands. International Journal of Oral Science, 8(1), 16-23.
  • Reference14. Özler, M., Şimşek, K., Topal, T., Öter, Ş. ve Korkmaz, A. (2010). Pinealektomili Ratlarda Yara İyileşmesi. Gülhane Tıp Dergisi, 52, 181-184. Reference15. Luft, R. and Minkowski, O. (1989). Discovery of the pancreatic origin of diyabetes. Diyabetologia, 32(7), 399-401.
  • Reference16. Baynes, J. W., Thorpe, S. R. (1999). Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. Diabetes, 48(1), 1–9.
  • Reference17. Dandona, P., Thusu, K., Cook, S., Snyder, B., Makowski, J., Armstrong, D. and Nicotera, T. (1996). Oxidative damage to DNA in diabetes mellitus. The Lancet, 347(8999), 444-445.
  • Reference18. Aslan, D., Onat, T., Emerk, K. ve Sözmen, E. Y., (2006). İnsan Biyokimyası (İkinci Baskı), Ankara: Palme Yayıncılık. 691-699, 714.
  • Reference19. Pitocco, D., Zaccardi, F., Di Stasio, E., Romitelli, F., Santini, S. A., Zuppi, C., Ghirlanda, G. (2010). Oxidative stress, nitric oxide and diabetes. The review of Diabetic Studies, 7(1), 15-25
  • Reference20. Kurtel, H., Granger, D. N., Tso, P., Grisham, M. B. (1992). Vulnerability of intestinal interstitial fluid to oxidant stress. American Journal of Physiology-Gastrointestinal and Liver Physiology, 263(4), 573-578.
  • Reference21. Miranda, K. M., Espey, M. G., Wink, D. A. (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric oxide, 5(1), 62-71.
  • Reference22. Aydemir, B., Sarı, E.K. (2009). Antioksidanlar ve Büyüme Faktörleri ile İlişkisi, Kocatepe Veteriner Dergisi, 2(2), 56-60.
  • Reference23. Altan, N., Dinçel, A. S., and Koca, C. (2006). Diabetes Mellitus ve Oksidatif Stres, Türkiye Biyokimya Dergisi., 31(2), 51-56.
  • Reference24. Çetin, M. ve Çapan, Y. (2004). Bazik Fibroblast Büyüme Faktörü ve Formülasyonlarında Yeni Yaklaşımlar, Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, 24(2), 107-124.
  • Reference25. Yazıcı, A. E., Paşaoğlu, H., Yücel, D., Çelebi, N., Bakır, F., Özkaya, M. (2002). Tip II Diabetes Mellituslu Hastalarda Plazma Total Tiyol ve Eritrosit Redükte Glutatyon Düzeyleri. Turkiye Klinikleri Journal of Medical Sciences, 22(5), 487-492.
  • Reference26. Hu, M. L. (1994). Measurement of protein thiol groups and glutathione in plasma. In Methods in enzymology. 233, 380-385
  • Reference27. Karabağ, F. (2010). Deneysel Olarak Hipertiroidizm Olusturulan Ratlarda Kafeik Asit Fenetil Ester’in Plazma Homosistein, Asimetrik Dimetil Arjinin, Nitrik Oksit ve Lipid Profili Üzerine Etkilerinin Araştırılması, Doktora Tezi, Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Afyonkarahisar, 16-17.
  • Reference28. Chin, G. A., Diegelmann, R. F., Schultz, G. S. (2005). Cellular and molecular regulation of wound healing. Basic and Clinical Dermatology, 17-37.
  • Reference29. Memişoğulları, R., (2006). Diyabette serbest radikallerin rolü ve antioksidanların etkisi, Düzce Tıp Fakültesi Dergisi, 3, 30-39.

Details

Primary Language English
Subjects Basic Sciences
Journal Section Research Articles
Authors

Esra OĞUZ (Primary Author)
GAZI UNIVERSITY, INSTITUTE OF HEALTH SCIENCES
0000-0002-5203-1445
Türkiye


Şule Coşkun CEVHER
GAZI UNIVERSITY, FACULTY OF SCIENCE
0000-0001-6204-2845
Türkiye

Supporting Institution TÜBİTAK
Project Number 215Z482
Publication Date December 31, 2021
Application Date July 12, 2021
Acceptance Date September 26, 2021
Published in Issue Year 2021, Volume 8, Issue 4

Cite

Bibtex @research article { hjse970153, journal = {Hittite Journal of Science and Engineering}, issn = {}, eissn = {2148-4171}, address = {Hitit Üniversitesi Mühendislik Fakültesi Kuzey Kampüsü Çevre Yolu Bulvarı 19030 Çorum / TÜRKİYE}, publisher = {Hitit University}, year = {2021}, volume = {8}, pages = {299 - 305}, doi = {10.17350/HJSE19030000242}, title = {Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats}, key = {cite}, author = {Oğuz, Esra and Cevher, Şule Coşkun} }
APA Oğuz, E. & Cevher, Ş. C. (2021). Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats . Hittite Journal of Science and Engineering , 8 (4) , 299-305 . DOI: 10.17350/HJSE19030000242
MLA Oğuz, E. , Cevher, Ş. C. "Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats" . Hittite Journal of Science and Engineering 8 (2021 ): 299-305 <https://dergipark.org.tr/en/pub/hjse/issue/67751/970153>
Chicago Oğuz, E. , Cevher, Ş. C. "Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats". Hittite Journal of Science and Engineering 8 (2021 ): 299-305
RIS TY - JOUR T1 - Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats AU - Esra Oğuz , Şule Coşkun Cevher Y1 - 2021 PY - 2021 N1 - doi: 10.17350/HJSE19030000242 DO - 10.17350/HJSE19030000242 T2 - Hittite Journal of Science and Engineering JF - Journal JO - JOR SP - 299 EP - 305 VL - 8 IS - 4 SN - -2148-4171 M3 - doi: 10.17350/HJSE19030000242 UR - https://doi.org/10.17350/HJSE19030000242 Y2 - 2021 ER -
EndNote %0 Hittite Journal of Science and Engineering Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats %A Esra Oğuz , Şule Coşkun Cevher %T Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats %D 2021 %J Hittite Journal of Science and Engineering %P -2148-4171 %V 8 %N 4 %R doi: 10.17350/HJSE19030000242 %U 10.17350/HJSE19030000242
ISNAD Oğuz, Esra , Cevher, Şule Coşkun . "Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats". Hittite Journal of Science and Engineering 8 / 4 (December 2021): 299-305 . https://doi.org/10.17350/HJSE19030000242
AMA Oğuz E. , Cevher Ş. C. Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats. Hittite J Sci Eng. 2021; 8(4): 299-305.
Vancouver Oğuz E. , Cevher Ş. C. Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats. Hittite Journal of Science and Engineering. 2021; 8(4): 299-305.
IEEE E. Oğuz and Ş. C. Cevher , "Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats", Hittite Journal of Science and Engineering, vol. 8, no. 4, pp. 299-305, Dec. 2021, doi:10.17350/HJSE19030000242