Research Article
PDF Zotero Mendeley EndNote BibTex Cite

Year 2021, Volume 8, Issue 4, 307 - 312, 31.12.2021
https://doi.org/10.17350/HJSE19030000243

Abstract

References

  • 1. Alp M, Alp A.S. Medisinal Kimyaya Kısa Bir Giriş, 1st ed. Akademisyen Kitabevi, Ankara, 2019.
  • 2. Ala AA, Olotu BB, Ohia CMD. Assessment of cytotoxicity of leaf extracts of Andrographis paniculata and Aspilia africana on murine cells in vitro. Archives of Basic and Applied Medicine 6 (2018) 61–65.
  • 3. Evstatieva L, Tchorbanov B. Complex Investigations of Tribulus Terrestris L. for Sustainable use by Pharmaceutical Industry. Biotechnology & Biotechnological Equipment 25 (2011) 2341–2347.
  • 4. Hashim S, Bakht T, Marwat KB, Jan A. Medicinal Properties, Phytochemistry and Pharmacology of Tribulus Terrestris L. (Zygophyllaceae). Pakistan Journal of Botany 46 (2014) 399–404.
  • 5. Angelova S, Gospodinova Z, Krasteva M, Antov G, Lozanov V, Markov T, Bozhanov S, Georgieva EI, Mitev V. Antitumor activity of Bulgarian herb Tribulus terrestris L. on human breast cancer cells. Journal of BioScience and Biotechnology 2 (2013) 25–32.
  • 6. Kang LP, Wu KL, Yu HS, Pang X, Liu J, Han LF, Zhang J, Zhao Y, Xiong CQ, Song XB, Liu C, Cong YW, Ma BP. Steroidal saponins from Tribulus terrestris. Phytochemistry 107 (2014) 182–189.
  • 7. Neychev VK, Nikolova E, Zhelev N, Mitev VI. Saponins from Tribulus terrestris L. Are Less Toxic for Normal Human Fibroblasts than for Many Cancer Lines: Influence on Apoptosis and Proliferation. Experimental Biology and Medicine 232 (2007) 126–33.
  • 8. Sun B, Qu W, Bai Z. [The inhibitory effect of saponins from Tribulus terrestris on Bcap-37 breast cancer cell line in vitro]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials 26 (2003) 104–106.
  • 9. Bedir E, Khan IA. New Steroidal Glycosides from the Fruits of Tribulus terrestris. Journal of Natural Products 63 (2000) 1699–1701.
  • 10. Sun B, Qu W, Zhang X, Yang H, Zhuang X, Zhang P. [Investigation on inhibitory and apoptosis-inducing effects of saponins from Tribulus terrestris on hepatoma cell line BEL-7402]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica 29 (2004) 681–684.
  • 11. van Meerloo J, Kaspers GJL, Cloos J. Cell sensitivity assays: the MTT assay. Methods in Molecular Biology (Clifton, N.J.) 731 (2011) 237–245.
  • 12. Al Jitan S, Alkhoori SA, Yousef L F. Phenolic Acids from Plants: Extraction and Application to Human Health. Studies in Natural Products Chemistry 58 (2018) 389–417.
  • 13. Ötleş S, ed. Handbook of Food Analysis Instruments. CRC Press, Boca Raton, 2009.
  • 14. Ammar NM, El-Din El-Hawary SS, Mohamed DA, Afifi MS, Ghanem DM, Awad G, Research Article Phytochemical and Biological Studies of Tribulus terrestris L. Growing in Egypt. International Journal of Pharmacology 14 (2) (2018) 248-259.
  • 14. Wang J, Zu X, Jiang Y. Five furostanol saponins from fruits of Tribulus terrestris and their cytotoxic activities. Natural Product Research 23 (2009) 1436–1444.
  • 16. Abudayyak M, Jannuzzi AT, Özhan G, Alpertunga B. Investigation on the toxic potential of Tribulus terrestris in vitro. Pharmaceutical Biology 53 (2015) 469–476.
  • 17. Chauhan S, Sharma D, Goel HC. An in vitro evaluation of Tribulus terrestris L. fruit extract for exploring therapeutic potential against certain gut ailments. Indian Journal of Experimental Biology 56 (2018) 430–436.
  • 18. Pourali M, Yaghoobi MM, Salehi Sormaghi MH. Cytotoxic, Anti-Proliferative and Apoptotic Effects of Tribulus terrestris L. Fruit Extract on Human Prostate Cancer Lncap and Colon Cancer HT-29 Cell Lines. Jundishapur Journal of Natural Pharmaceutical Products 12 (2016) e33561.
  • 19. Oliveira Filho CC, Kampke EH, Vargas TS, Salustriano NA, Scherer R, Fronza M, et al. In vitro cytotoxic activity of five commercial samples of Tribulus terrestris Linn in Espírito Santo (Brazil). Brazilian Journal of Pharmaceutical Sciences 53(4) (2018) e00262.
  • 20. Naz R, Ayub H, Nawaz S, Islam ZU, Yasmin T, Bano A, et al. Antimicrobial activity, toxicity and anti-inflammatory potential of methanolic extracts of four ethnomedicinal plant species from Punjab, Pakistan. BMC Complementary and Alternative Medicine 2017;17(1):302.
  • 21. Menon D, Dharmapal S, Raghavamenon A, Babu T. ~ 1 ~ Cytotoxic and antitumor effects of Tribulus terrestris L fruit methanolic extract. Journal of Pharmacognosy and Phytochemistry 3(2) (2014) 1–4.
  • 22. Gacche RN, Dhole NA. Profile of aldose reductase inhibition, anti-cataract and free radical scavenging activity of selected medicinal plants: An attempt to standardize the botanicals for amelioration of diabetes complications. Food and Chemical Toxicology 2011;49(8):1806–1813.
  • 23. Patel A, Soni A, Siddiqi NJ, Sharma P. An insight into the anticancer mechanism of Tribulus terrestris extracts on human breast cancer cells. 3 Biotech 9(2) (2019) 58.
  • 24. Fieldsend AF, Morison JIL. Climatic conditions during seed growth significantly influence oil content and quality in winter and spring evening primrose crops (Oenothera spp.). Industrial Crops and Products 12 (2000) 137–147.
  • 25. Endes Z, Er F, Özcan MM, Juhaimi FA. Oil Content, Saturated and Unsaturated Fatty Acids of Some Linseed (Linum usitatissimum L.) Genotypes. Asian Journal of Chemistry 25 (2013) 9285–9287.
  • 26. International Organization for Standardization. Biological evaluation of medical devices – ISO 10993, Part 5: Tests for in vitro cytotoxicity. Geneva, Switzerland; 1999.

Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay

Year 2021, Volume 8, Issue 4, 307 - 312, 31.12.2021
https://doi.org/10.17350/HJSE19030000243

Abstract

People, especially those living in rural environments and those who have recently been dissatisfied with traditional medicine, use medicinal plants for their therapeutic effects. In this study, ethanol, ethyl acetate and methanol extracts of Tribulus terrestris L. plant, which is used by local people as one of these medicinal plants in Kars province (Turkey), were obtained by ultrasound assisted extraction method. The cytotoxic effects of the obtained extracts on MCF-7 breast cancer cells in the concentration range of 10-1000 ppm at 24 h of exposure were investigated by the MTT method, which is a colorimetric method. T. terrestris L. ethanol extracts at 1000 ppm caused a moderate cytotoxic effect on MCF-7 breast cancer cells. It was determined that ethanolic extracts in the concentration range of 10-500 ppm and methanol and ethyl acetate extracts in the concentration of 10-1000 ppm caused cell proliferation.

References

  • 1. Alp M, Alp A.S. Medisinal Kimyaya Kısa Bir Giriş, 1st ed. Akademisyen Kitabevi, Ankara, 2019.
  • 2. Ala AA, Olotu BB, Ohia CMD. Assessment of cytotoxicity of leaf extracts of Andrographis paniculata and Aspilia africana on murine cells in vitro. Archives of Basic and Applied Medicine 6 (2018) 61–65.
  • 3. Evstatieva L, Tchorbanov B. Complex Investigations of Tribulus Terrestris L. for Sustainable use by Pharmaceutical Industry. Biotechnology & Biotechnological Equipment 25 (2011) 2341–2347.
  • 4. Hashim S, Bakht T, Marwat KB, Jan A. Medicinal Properties, Phytochemistry and Pharmacology of Tribulus Terrestris L. (Zygophyllaceae). Pakistan Journal of Botany 46 (2014) 399–404.
  • 5. Angelova S, Gospodinova Z, Krasteva M, Antov G, Lozanov V, Markov T, Bozhanov S, Georgieva EI, Mitev V. Antitumor activity of Bulgarian herb Tribulus terrestris L. on human breast cancer cells. Journal of BioScience and Biotechnology 2 (2013) 25–32.
  • 6. Kang LP, Wu KL, Yu HS, Pang X, Liu J, Han LF, Zhang J, Zhao Y, Xiong CQ, Song XB, Liu C, Cong YW, Ma BP. Steroidal saponins from Tribulus terrestris. Phytochemistry 107 (2014) 182–189.
  • 7. Neychev VK, Nikolova E, Zhelev N, Mitev VI. Saponins from Tribulus terrestris L. Are Less Toxic for Normal Human Fibroblasts than for Many Cancer Lines: Influence on Apoptosis and Proliferation. Experimental Biology and Medicine 232 (2007) 126–33.
  • 8. Sun B, Qu W, Bai Z. [The inhibitory effect of saponins from Tribulus terrestris on Bcap-37 breast cancer cell line in vitro]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials 26 (2003) 104–106.
  • 9. Bedir E, Khan IA. New Steroidal Glycosides from the Fruits of Tribulus terrestris. Journal of Natural Products 63 (2000) 1699–1701.
  • 10. Sun B, Qu W, Zhang X, Yang H, Zhuang X, Zhang P. [Investigation on inhibitory and apoptosis-inducing effects of saponins from Tribulus terrestris on hepatoma cell line BEL-7402]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica 29 (2004) 681–684.
  • 11. van Meerloo J, Kaspers GJL, Cloos J. Cell sensitivity assays: the MTT assay. Methods in Molecular Biology (Clifton, N.J.) 731 (2011) 237–245.
  • 12. Al Jitan S, Alkhoori SA, Yousef L F. Phenolic Acids from Plants: Extraction and Application to Human Health. Studies in Natural Products Chemistry 58 (2018) 389–417.
  • 13. Ötleş S, ed. Handbook of Food Analysis Instruments. CRC Press, Boca Raton, 2009.
  • 14. Ammar NM, El-Din El-Hawary SS, Mohamed DA, Afifi MS, Ghanem DM, Awad G, Research Article Phytochemical and Biological Studies of Tribulus terrestris L. Growing in Egypt. International Journal of Pharmacology 14 (2) (2018) 248-259.
  • 14. Wang J, Zu X, Jiang Y. Five furostanol saponins from fruits of Tribulus terrestris and their cytotoxic activities. Natural Product Research 23 (2009) 1436–1444.
  • 16. Abudayyak M, Jannuzzi AT, Özhan G, Alpertunga B. Investigation on the toxic potential of Tribulus terrestris in vitro. Pharmaceutical Biology 53 (2015) 469–476.
  • 17. Chauhan S, Sharma D, Goel HC. An in vitro evaluation of Tribulus terrestris L. fruit extract for exploring therapeutic potential against certain gut ailments. Indian Journal of Experimental Biology 56 (2018) 430–436.
  • 18. Pourali M, Yaghoobi MM, Salehi Sormaghi MH. Cytotoxic, Anti-Proliferative and Apoptotic Effects of Tribulus terrestris L. Fruit Extract on Human Prostate Cancer Lncap and Colon Cancer HT-29 Cell Lines. Jundishapur Journal of Natural Pharmaceutical Products 12 (2016) e33561.
  • 19. Oliveira Filho CC, Kampke EH, Vargas TS, Salustriano NA, Scherer R, Fronza M, et al. In vitro cytotoxic activity of five commercial samples of Tribulus terrestris Linn in Espírito Santo (Brazil). Brazilian Journal of Pharmaceutical Sciences 53(4) (2018) e00262.
  • 20. Naz R, Ayub H, Nawaz S, Islam ZU, Yasmin T, Bano A, et al. Antimicrobial activity, toxicity and anti-inflammatory potential of methanolic extracts of four ethnomedicinal plant species from Punjab, Pakistan. BMC Complementary and Alternative Medicine 2017;17(1):302.
  • 21. Menon D, Dharmapal S, Raghavamenon A, Babu T. ~ 1 ~ Cytotoxic and antitumor effects of Tribulus terrestris L fruit methanolic extract. Journal of Pharmacognosy and Phytochemistry 3(2) (2014) 1–4.
  • 22. Gacche RN, Dhole NA. Profile of aldose reductase inhibition, anti-cataract and free radical scavenging activity of selected medicinal plants: An attempt to standardize the botanicals for amelioration of diabetes complications. Food and Chemical Toxicology 2011;49(8):1806–1813.
  • 23. Patel A, Soni A, Siddiqi NJ, Sharma P. An insight into the anticancer mechanism of Tribulus terrestris extracts on human breast cancer cells. 3 Biotech 9(2) (2019) 58.
  • 24. Fieldsend AF, Morison JIL. Climatic conditions during seed growth significantly influence oil content and quality in winter and spring evening primrose crops (Oenothera spp.). Industrial Crops and Products 12 (2000) 137–147.
  • 25. Endes Z, Er F, Özcan MM, Juhaimi FA. Oil Content, Saturated and Unsaturated Fatty Acids of Some Linseed (Linum usitatissimum L.) Genotypes. Asian Journal of Chemistry 25 (2013) 9285–9287.
  • 26. International Organization for Standardization. Biological evaluation of medical devices – ISO 10993, Part 5: Tests for in vitro cytotoxicity. Geneva, Switzerland; 1999.

Details

Primary Language English
Subjects Basic Sciences
Journal Section Research Articles
Authors

Giray Buğra AKBABA
KAFKAS ÜNİVERSİTESİ
0000-0002-1413-9498
Türkiye


Füreya Elif ÖZTÜRKKAN (Primary Author)
Kafkas Üniversitesi
0000-0001-6376-4161
Türkiye


Mustafa SERTÇELİK
KAFKAS ÜNİVERSİTESİ
0000-0001-7919-7907
Türkiye

Thanks The authors are thanked to Yıldırım Öztürkkan and Kenan Kara for their help in the collection of plant samples, and to Prof. Dr. Fatma Güneş for their support in plant identification, to Prof. Dr. Bingür Sönmez and Prof. Dr. Fikrettin Şahin for their support in the supply of cell lines, and to Prof. Dr. Hacali Necefoğlu, Dr. Cem Öziç, Dr. Yunus Ensari and Didem Yıldırım for their technical support.
Publication Date December 31, 2021
Application Date July 16, 2021
Acceptance Date November 20, 2021
Published in Issue Year 2021, Volume 8, Issue 4

Cite

Bibtex @research article { hjse972522, journal = {Hittite Journal of Science and Engineering}, issn = {}, eissn = {2148-4171}, address = {Hitit Üniversitesi Mühendislik Fakültesi Kuzey Kampüsü Çevre Yolu Bulvarı 19030 Çorum / TÜRKİYE}, publisher = {Hitit University}, year = {2021}, volume = {8}, pages = {307 - 312}, doi = {10.17350/HJSE19030000243}, title = {Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay}, key = {cite}, author = {Akbaba, Giray Buğra and Öztürkkan, Füreya Elif and Sertçelik, Mustafa} }
APA Akbaba, G. B. , Öztürkkan, F. E. & Sertçelik, M. (2021). Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay . Hittite Journal of Science and Engineering , 8 (4) , 307-312 . DOI: 10.17350/HJSE19030000243
MLA Akbaba, G. B. , Öztürkkan, F. E. , Sertçelik, M. "Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay" . Hittite Journal of Science and Engineering 8 (2021 ): 307-312 <https://dergipark.org.tr/en/pub/hjse/issue/67751/972522>
Chicago Akbaba, G. B. , Öztürkkan, F. E. , Sertçelik, M. "Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay". Hittite Journal of Science and Engineering 8 (2021 ): 307-312
RIS TY - JOUR T1 - Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay AU - Giray Buğra Akbaba , Füreya Elif Öztürkkan , Mustafa Sertçelik Y1 - 2021 PY - 2021 N1 - doi: 10.17350/HJSE19030000243 DO - 10.17350/HJSE19030000243 T2 - Hittite Journal of Science and Engineering JF - Journal JO - JOR SP - 307 EP - 312 VL - 8 IS - 4 SN - -2148-4171 M3 - doi: 10.17350/HJSE19030000243 UR - https://doi.org/10.17350/HJSE19030000243 Y2 - 2021 ER -
EndNote %0 Hittite Journal of Science and Engineering Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay %A Giray Buğra Akbaba , Füreya Elif Öztürkkan , Mustafa Sertçelik %T Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay %D 2021 %J Hittite Journal of Science and Engineering %P -2148-4171 %V 8 %N 4 %R doi: 10.17350/HJSE19030000243 %U 10.17350/HJSE19030000243
ISNAD Akbaba, Giray Buğra , Öztürkkan, Füreya Elif , Sertçelik, Mustafa . "Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay". Hittite Journal of Science and Engineering 8 / 4 (December 2021): 307-312 . https://doi.org/10.17350/HJSE19030000243
AMA Akbaba G. B. , Öztürkkan F. E. , Sertçelik M. Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay. Hittite J Sci Eng. 2021; 8(4): 307-312.
Vancouver Akbaba G. B. , Öztürkkan F. E. , Sertçelik M. Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay. Hittite Journal of Science and Engineering. 2021; 8(4): 307-312.
IEEE G. B. Akbaba , F. E. Öztürkkan and M. Sertçelik , "Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay", Hittite Journal of Science and Engineering, vol. 8, no. 4, pp. 307-312, Dec. 2021, doi:10.17350/HJSE19030000243