Selective Recognition of Kanamycin via Molecularly Imprinted Nanosensor

Year 2022, Volume: 9 Issue: 1, 1 - 7, 30.03.2022

Esma Sari

https://doi.org/10.17350/HJSE19030000249

Cited By: 1

Abstract

Herein, the molecular recognition sites on the surface of the chip were created by the molecular imprinting method to produce the surface plasmon resonance (SPR) based nanosensor for the real-time kanamycin (KAN) detection. Firstly, kanamycin imprinted nanofilm, which has specific recognition cavities for kanamycin were synthesized by in-situ radical polymerization. Fabricated nanofilm for the detection of kanamycin was characterized with FTIR, ellipsometer, and atomic force microscope by the means of structurally and morphologically. The mean thickness values were determined for the imprinted and non-imprinted nanofilms as 102.4±3.1 nm and 101.8±4.7, respectively. The sensitivity performance of imprinted nanosensor was investigated by using the KAN solutions at different concentrations (25-200 ng/mL). The refractive index and the KAN concentration were found to be in perfect agreement with a regression coefficient (R2, 0.992). The detection limit was calculated as 0.40±0.05 ng/mL by using the equation in the calibration curve. The response of imprinted and nonimprinted nanosensors towards the chemical analogs of KAN (NEO and SPM) were investigated to prove the selectivity of KAN imprinted nanosensors. The reusability performance of imprinted nanosensor was investigated by spiking 25 ng/mL KAN solution with three replicates. When the kinetic analyzes were examined, high sensitivity real-time kanamycin analysis was performed at very low concentrations with good reusability.

Keywords

kanamycin, surface plasmon resonance, molecular imprinting, nanosensor

References

• 1. Claes PJ, Dubost M, Vanderhaeghe H. Kanamycin sulfate, Analytical Profiles of Drug Substances., New York, pp. 259–296, 1977.
• 2. Sharma N, Selvam SP, Yun K. Electrochemical detection of amikacin sulphate using reduced graphene oxide and silver nanoparticles nanocomposite. Applied Surface Science 512 (2020) 145742.
• 3. Xu Y, Han T, Li X, Sun L, Zhang Y, Zhang Y. Colorimetric detection of kanamycin based on analyte-protected silver nanoparticles and aptamer-selective sensing mechanism. Analytica Chimica Acta 891 (2015) 298-303.
• 4. Rahman M M. Chemical, Label-free Kanamycin sensor development based on CuONiO hollow-spheres: food samples analyses. Sensors and Actuators B: Chemical 264 (2018) 84-91. 5. Bai X, Hou H, Zhang B, Tang J. Label-free detection of kanamycin using aptamer-based cantilever array sensor. Biosensors and Bioelectronics 56 (2014) 112-116.
• 6. Wang R, Wang R, Ge B, Jia X, Li Z, Chang J. Spectral method determination of kanamycin sulfate using both gold nanoparticles and quantum dots. Analytical Methods 19 (2013) 5302-5308.
• 7. Long F, Zhang Z, Yang Z, Zeng J, Jiang Y. Imprinted electrochemical sensor based on magnetic multi-walled carbon nanotube for sensitive determination of kanamycin. Journal of Electroanalytical Chemistry 755 (2015) 7-14.
• 8. Adams E, Dalle J, De Bie E, De Smedt I, Roets E, Hoogmartens J. Analysis of kanamycin sulfate by liquid chromatography with pulsed electrochemical detection. Journal of Chromatography A 766 (1997) 133-139.
• 9. Tao Y, Chen D, Yu H, Huang L, Liu Z, Cao X, Yan C, Pan Y, Liu Z, Yuan Z. Simultaneous determination of 15 aminoglycoside (s) residues in animal derived foods by automated solid-phase extraction and liquid chromatography–tandem mass spectrometry. Food Chemistry 135 (2012) 676-683.
• 10. Song HY, Kang TF, Li NN, Lu LP, Cheng SY. Highly sensitive voltammetric determination of kanamycin based on aptamer sensor for signal amplification. Analytical Methods 16 (2016) 3366-3372.
• 11. Lin YF, Wang YC, Chang SY. Capillary electrophoresis of aminoglycosides with argon-ion laser-induced fluorescence detection. Journal of Chromatography A 1188 (2008) 331-333.
• 12. Kaale E, Van Schepdael A, Roets E, Hoogmartens J. Development and validation of a simple capillary zone electrophoresis method for the analysis of kanamycin sulfate with UV detection after pre-capillary derivatization. Journal of Chromatography A 924 (2001) 451-458.
• 13. Arabi M, Ostovan A, Li J, Wang X, Zhang Z, Choo J, Chen L. Molecular imprinting: green perspectives and strategies. Advanced Materials 33 (2021) 2100543.
• 14. Mosbach K. Molecular imprinting. Trends in biochemical sciences 19 (1994) 9-14.
• 15. Mosbach K, Ramström O. The emerging technique of molecular imprinting and its future impact on biotechnology. Bio/technology 14 (1996) 163-170.
• 16. Wulff G. Molecular imprinting in cross‐linked materials with the aid of molecular templates—a way towards artificial antibodies. Angewandte Chemie International Edition in English 34 (1995) 1812-1832.
• 17. He S, Zhang L, Bai S, Yang H, Cui Z, Zhang X, Li Y. Advances of molecularly imprinted polymers (MIP) and the application in drug delivery. European Polymer Journal 143 (2021) 110179.
• 18. Piletsky SS, Piletska E, Poblocka M, Macip S, Jones DJL, Braga M, Cao TH, Singh R, Spivey AC, Aboagye EO, Piletsky SA. Snapshot imprinting: rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers. Nano Today 41 (2021) 101304.
• 19. Zhang J, Wang Y, Lu X. Molecular imprinting technology for sensing foodborne pathogenic bacteria. Analytical and Bioanalytical Chemistry 413 (2021) 4581-4598.
• 20. Balayan S, Chauhan N, Chandra R, Jain U. Molecular imprinting based electrochemical biosensor for identification of serum amyloid A (SAA), a neonatal sepsis biomarker. International Journal of Biological Macromolecules 195 (2022) 589-597.
• 21. Perez-Puyana V, Wieringa P, Guerrero A, Romero A, Moroni L. (Macro) Molecular Imprinting of Proteins on PCL Electrospun Scaffolds. ACS Applied Materials & Interfaces 13.25 (2021) 29293-29302.
• 22. Bonatti AF, De Maria C, Vozzi G. Molecular imprinting strategies for tissue engineering applications: A review. Polymers 13 (2021) 548.
• 23. Cennamo N, D'Agostino G, Pesavento M, Zeni L. High selectivity and sensitivity sensor based on MIP and SPR in tapered plastic optical fibers for the detection of L-nicotine. Sensors and Actuators B: Chemical 191 (2014) 529-536.
• 24. Matsui J, Akamatsu K, Hara N, Miyoshi D, Nawafune H, Tamaki K, Sugimoto N. SPR sensor chip for detection of small molecules using molecularly imprinted polymer with embedded gold nanoparticles. Analytical Chemistry 77 (2005) 4282-4285.
• 25. Shrivastav AM, Mishra SK, Gupta BD. Fiber optic SPR sensor for the detection of melamine using molecular imprinting. Sensors and Actuators B: Chemical 212 (2015) 404-410.
• 26. Meneghello A, Sonato A, Ruffato G, Zacco G, Romanato F. A novel high sensitive surface plasmon resonance Legionella pneumophila sensing platform. Sensors and Actuators B: Chemical 250 (2017) 351-355.
• 27. Bognár Z, Supala E, Yarman A, Zhang X, Bier FF, Scheller FW, Gyurcsányi RE. Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein. Chemical Science 13 (2022) 1263-1269.
• 28. Sinha RK. Wavelength modulation based surface plasmon resonance sensor for detection of cardiac marker proteins troponin I and troponin T. Sensors and Actuators A: Physical 332 (2021) 113104.
• 29. Jagirani MS, Mahesar SA, Uddin S, Sherazi STH, Kori AH, Lakho SA, Kalwar NH, Memon SS. Functionalized Gold Nanoparticles Based Optical, Surface Plasmon Resonance-Based Sensor for the Direct Determination of Mitoxantrone Anti-cancer Agent from Real Samples. Journal of Cluster Science 33 (2022) 241-247.
• 30. Wang W, Wang R, Liao M, Kidd MT, Li Y. Rapid detection of enrofloxacin using a localized surface plasmon resonance sensor based on polydopamine molecular imprinted recognition polymer. Journal of Food Measurement and Characterization 15 (2021) 3376-3386.
• 31. Sari E, Üzek R, Duman M, Denizli A. Detection of ciprofloxacin through surface plasmon resonance nanosensor with specific recognition sites. Journal of Biomaterials science, Polymer edition, 29 (2018) 1302-1318.
• 32. Sari E, Üzek R, Duman M, Denizli A. Fabrication of surface plasmon resonance nanosensor for the selective determination of erythromycin via molecular imprinted nanoparticles. Talanta 150 (2016) 607-614.
• 33. Sari E, Üzek R, Duman M, Alagöz HY, Denizli A. Prism coupler-based sensor system for simultaneous screening of synthetic glucocorticosteroid as doping control agent. Sensors and Actuators B: Chemical 260 (2018) 432-444.