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Pathological effects of Helleborus Orientalis used in Turkish traditional medicine, in rats

Year 2017, , 71 - 75, 28.12.2017
https://doi.org/10.30704/http-www-jivs-net.362297

Abstract

elleborus orientalis Lam., a member of the family Ranunculaceae, is one of the medical plants, which is naturally grown in Turkey flora. Roots of Helleborus orientalis are used for the treatment of farm animals by implanting subcutaneously in the public. This study was planned to investigate the histopathological changes in liver, heart and skin of rats that were applied plant raw material (PRM), water extract (WE) and methanol extracts (ME) of Helleborus orientalis subcutaneously. Foci of abscess were determined in the subcutaneous tissue of all 3 experimental groups at the end of 10-day experimental period. During the histopathological examination of the liver, parenchymatous degenerations were observed in the hepatocytes of PRM and WE group rats, and caseous necrosis were determined in the liver of all rats in ME group. Hyaline degenerations were observed in myocard cells of the rats in PRM and WE groups. Furthermore hyaline degenerations and edema occurred locally in the myocard of all animals in ME group. Vacuolar zones were found in certain myocard cells. The results of the present study indicate that long-term use of Helleborus orientalis might be harmful due to its pathological effects on some tissue.

References

  • Blanco, E., Macia, M.J., Morales, R., 1999. Medicinal and veterinary plants of El Caurel (Galicia, northwest Spain). Journal of Ethnopharmacology 65, 113-124.
  • Bogdan, İ., Nechifor, A., Başea, I., Hruban, E., 1990. From the Rumanian folk medicine: unspecific irritating therapy by transcutaneous implantation of Helleborus purpurascens Fam. Ranunculaceae in farm animals. Deutsche Tierarztliche Wochenschrift 97, 525-529.
  • Büssing, A., Schweizer, K., 1998. Effects of a phytopreparation from Helleborus niger on immunocompetent cells in vitro. Journal of Ethnopharmacology 59, 139-146.
  • Cioaca, C., Cucu, V., 1974. Quantitative determination of hellebrin in the rhizomes and roots of Helleborus purpurascens W.et K. Planta Medica 26, 250-253.
  • Duncan, J.R., Prasse K.W.,1986. Veterinary laboratory medicine clinical pathology 2nd ed. Iowa State University Press, Ames Iowa. pp. 121-136.
  • Erdemoglu, N., Küpeli, E., Yeşilada, E., 2003. Anti-inflammatory and antinociceptive activity assessment of plants used as remedy in Turkish folk medicine. Journal of Ethnopharmacology 89, 123-129.
  • Holliman, A., Milton, D., 1990. Helleborus foetidus poisoning of cattle. The Veterinary. Record 127, 339-340.
  • Kantemir, İ., Temelli, E., 1963. Comparative studies with Helleborus orientalis L. Arzneimittelforschung 13, 1012-1013.
  • Linke, S., Dufter, C., Kerek, F., Jung, M., Watzlik, A., Jung, T., Opelz, G., Terness, P., 1998. Functional characterization of HP12: A novel immunosuppressant purified from Helleborus species. Transplantation Proceedings 30, 4106-4107.
  • Luna, L.G., 1968. Manual of histologic staining methods of the Armed Forces Institute of Pathology, 3rd ed. Mc Graw-Hill Book Company, New York. 32-46.
  • Malkoç, G., 1967. Pharmacognosic investigations in Helleborus orientalis Lam var. Hirsitus (schıffn) hay. plant. Ph.D. Thesis (Adviser: Prof. Dr. Sarım Çelebioğlu) Department of Pharmacognosy, Faculty of Pharmacy, Istanbul University. Istanbul. Turkey.
  • Matur, E., Özcan, M., Turan Çıraklı, Z., Küçükyalçın, E., 2003. Bioactivity examination of Helleborus orientalis plant in rats. 1. Effects on some physiological parameters. Indian Veterinary Journal 80, 1239-1244.
  • Matur, E., Turan Çıraklı, Z., Özcan, M., Kadıköylü, Ç., 2004. Bioactivity examination of Helleborus orientalis plant in rats. 2. Effects on some serum parameters. Indian Veterinary Journal 81, 387- 391.
  • Meng, Y., Whiting, P., Sik, V., Rees, H.H., Dinan, L., 2001. Ecdysteroids and bufadienolides from Helleborus torquatus (Ranunculaceae). Phytochemistry 57, 401-407.
  • Pascual-Villalobos, M.J., Robledo, A., 1998. Screening for anti-insect activity in Mediterranean plants. Industrial Crops and Products 8, 183-194.
  • Terness, P., Dufter, C., Linke, S., Kerek, F., Jung, T., Watzlik, A., Opelz G., 1999. HP12: A highly purified Helleborus species extract strongly potentiates the T-cell suppressive effect of cyclosporine A. Transplantation Proceedings 31, 1174-1175.
  • Turgut, K., 2000 Veterinary clinic laboratory diagnosis. 2nd ed. Bahcivanlar Press, Konya / Turkey, pp: 202-258.
  • Uncini Manganelli, R.E., Camangi, F., Tomei P.E., 2001. Curing animals with plants: traditional usage in Tuscany (Italy). Journal of Ethnopharmacology 78, 171-191.
  • Wissner, W., Kating, H., 1971. Studies on the hellebrin content in subterranean organs of Helleborus species. Planta Medica 20, 344-349.
  • Zhang, J., Meng, Z., Zhang, M., Ma, D., Xu, S., Kodama, H., 1999. Effect of six steroidal saponins isolated from Anemarrhenae rhizoma on platelet aggregation and hemolysis in human blood. Clinica Chimica Acta. 289, 79-88.

Pathological effects of Helleborus Orientalis used in Turkish traditional medicine, in rats

Year 2017, , 71 - 75, 28.12.2017
https://doi.org/10.30704/http-www-jivs-net.362297

Abstract



Helleborus orientalis Lam., a member of the
family Ranunculaceae, is one of the medical plants, which is naturally grown in
Turkey
flora. Roots of Helleborus orientalis are used for the treatment of farm
animals by implanting subcutaneously in the public. This study was planned to
investigate the histopathological changes in liver, heart and skin of rats that
were applied plant raw material (PRM), water extract (WE) and methanol extracts
(ME) of Helleborus orientalis subcutaneously. Foci of abscess were
determined in the subcutaneous tissue of all 3 experimental groups at the end
of 10-day experimental period. During the histopathological examination of the
liver, parenchymatous degenerations were observed in the hepatocytes of PRM and
WE group rats, and caseous necrosis were determined in the liver of all rats in
ME group. Hyaline degenerations were observed in myocard cells of the rats in
PRM and WE groups. Furthermore hyaline degenerations and edema occurred locally
in the myocard of all animals in ME group. Vacuolar zones were found in certain
myocard cells. The results of the present study indicate that long-term use of Helleborus
orientalis
might be harmful due to its pathological effects on some tissue.

References

  • Blanco, E., Macia, M.J., Morales, R., 1999. Medicinal and veterinary plants of El Caurel (Galicia, northwest Spain). Journal of Ethnopharmacology 65, 113-124.
  • Bogdan, İ., Nechifor, A., Başea, I., Hruban, E., 1990. From the Rumanian folk medicine: unspecific irritating therapy by transcutaneous implantation of Helleborus purpurascens Fam. Ranunculaceae in farm animals. Deutsche Tierarztliche Wochenschrift 97, 525-529.
  • Büssing, A., Schweizer, K., 1998. Effects of a phytopreparation from Helleborus niger on immunocompetent cells in vitro. Journal of Ethnopharmacology 59, 139-146.
  • Cioaca, C., Cucu, V., 1974. Quantitative determination of hellebrin in the rhizomes and roots of Helleborus purpurascens W.et K. Planta Medica 26, 250-253.
  • Duncan, J.R., Prasse K.W.,1986. Veterinary laboratory medicine clinical pathology 2nd ed. Iowa State University Press, Ames Iowa. pp. 121-136.
  • Erdemoglu, N., Küpeli, E., Yeşilada, E., 2003. Anti-inflammatory and antinociceptive activity assessment of plants used as remedy in Turkish folk medicine. Journal of Ethnopharmacology 89, 123-129.
  • Holliman, A., Milton, D., 1990. Helleborus foetidus poisoning of cattle. The Veterinary. Record 127, 339-340.
  • Kantemir, İ., Temelli, E., 1963. Comparative studies with Helleborus orientalis L. Arzneimittelforschung 13, 1012-1013.
  • Linke, S., Dufter, C., Kerek, F., Jung, M., Watzlik, A., Jung, T., Opelz, G., Terness, P., 1998. Functional characterization of HP12: A novel immunosuppressant purified from Helleborus species. Transplantation Proceedings 30, 4106-4107.
  • Luna, L.G., 1968. Manual of histologic staining methods of the Armed Forces Institute of Pathology, 3rd ed. Mc Graw-Hill Book Company, New York. 32-46.
  • Malkoç, G., 1967. Pharmacognosic investigations in Helleborus orientalis Lam var. Hirsitus (schıffn) hay. plant. Ph.D. Thesis (Adviser: Prof. Dr. Sarım Çelebioğlu) Department of Pharmacognosy, Faculty of Pharmacy, Istanbul University. Istanbul. Turkey.
  • Matur, E., Özcan, M., Turan Çıraklı, Z., Küçükyalçın, E., 2003. Bioactivity examination of Helleborus orientalis plant in rats. 1. Effects on some physiological parameters. Indian Veterinary Journal 80, 1239-1244.
  • Matur, E., Turan Çıraklı, Z., Özcan, M., Kadıköylü, Ç., 2004. Bioactivity examination of Helleborus orientalis plant in rats. 2. Effects on some serum parameters. Indian Veterinary Journal 81, 387- 391.
  • Meng, Y., Whiting, P., Sik, V., Rees, H.H., Dinan, L., 2001. Ecdysteroids and bufadienolides from Helleborus torquatus (Ranunculaceae). Phytochemistry 57, 401-407.
  • Pascual-Villalobos, M.J., Robledo, A., 1998. Screening for anti-insect activity in Mediterranean plants. Industrial Crops and Products 8, 183-194.
  • Terness, P., Dufter, C., Linke, S., Kerek, F., Jung, T., Watzlik, A., Opelz G., 1999. HP12: A highly purified Helleborus species extract strongly potentiates the T-cell suppressive effect of cyclosporine A. Transplantation Proceedings 31, 1174-1175.
  • Turgut, K., 2000 Veterinary clinic laboratory diagnosis. 2nd ed. Bahcivanlar Press, Konya / Turkey, pp: 202-258.
  • Uncini Manganelli, R.E., Camangi, F., Tomei P.E., 2001. Curing animals with plants: traditional usage in Tuscany (Italy). Journal of Ethnopharmacology 78, 171-191.
  • Wissner, W., Kating, H., 1971. Studies on the hellebrin content in subterranean organs of Helleborus species. Planta Medica 20, 344-349.
  • Zhang, J., Meng, Z., Zhang, M., Ma, D., Xu, S., Kodama, H., 1999. Effect of six steroidal saponins isolated from Anemarrhenae rhizoma on platelet aggregation and hemolysis in human blood. Clinica Chimica Acta. 289, 79-88.
There are 20 citations in total.

Details

Subjects Veterinary Surgery
Journal Section Research Articles
Authors

Gülbin Şennazlı

Erdal Matur

Publication Date December 28, 2017
Published in Issue Year 2017

Cite

APA Şennazlı, G., & Matur, E. (2017). Pathological effects of Helleborus Orientalis used in Turkish traditional medicine, in rats. Journal of Istanbul Veterinary Sciences, 1(3), 71-75. https://doi.org/10.30704/http-www-jivs-net.362297

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