Effects of probiotics on broilers exposed to Salmonella typhimurium

Year 2007, Issue: 1, 1 - 10, 08.05.2007

Serpil Kefalı Nezir Yaşar Toker

Abstract

Effects of probiotics
(Broilact, Bioplus 2B), which are used as an alternative to antibiotics and
chemical growth promoters, on broilers that were exposed to Salmonella
typhimurium (S. typhimurium) lipopolysaccharide (LPS) were studied. For
this purpose, 150 chicks for control group and 150 chicks for test group were
used resulting in a total of 300 Ross line broiler chicks. Each of control (C)
and test (T) group animals are further divided in to 3 subgroups as Check, Bro
and Bio. Test subgroups (T­Check, T­Bro, T­Bio) received 3 ml ofS.
typhimurium LPS intraperitonally (IP). T­Check animals received LPS only
while T­Bro received Broilact, a commercial probiotic, via drinking water on
day 1 after hatching only, and  T­Bio
animals received Bioplus 2B, another commercial probiotic, via feed during the
42­day study period. Control subgroups were not exposed to LPS but C­Bro and C­Bio
received probiotics same as the test subgroups (T­Check, T­Bro and T­Bio).
Effects of probiotics on the immune response of chickens against S.
typhimurium LPS were followed by slide agglutination test using
Salmonella "O" antigen. Results indicated that
percentage of Salmonella (+) animals increased in test subgroups
after LPS exposure. No difference was found between Broilact and Bioplus 2B in
regard to inhibiting Salmonella and supporting broiler's health. 

Keywords

Broyler, Salmonella typhimurium, lipopolisakkarit, probiyotik

References

• 1. Weinack O.M, Snoeyenbos G.H, Soerjadi­Liem A.S, Smyser C.F.: Influence of temperature, social, and dietary stress on development and stability of protective microflora in chickens against S. typhimurium. Avian Dis. 1985; 29 (4): 117-7­83.
• 2. Padron M.: Salmonella typhimurium outbreak in broiler chicken flocks in Mexico. Avian Dis. 1990 34 (1): 221­3.
• 3. Arda M., Minbay A., Aydın N., Akay Ö., İzgür M., Yardımcı H., Esendal Ö.M., Erdeğer J., Akan M.: Kanatlı Hayvan Hastalıkları. Medisan Yay Ser., 50, 1. Ed., 2002: 41­-53.
• 4. Yavuz M., ed. Çiftlik Hayvanlarının Beslenmesinde Temel Prensipler ve Karma Yem Üretiminde Bazı Bilimsel Yaklaşımlar. Farmavet İlaç San ve Tic A.Ş, İstanbul, 2001.
• 5. Vanbelle M., Teller E., Focant M.: Probiotics in animal nutrition: a review. Arc. Anim. Nutr. 1990; 40: 543-­556.
• 6. Jin L.Z., Ho Y.W., Abdullah N., Jalaludin S.: Probiotics in poultry: modes of action. World’s Poult. Sci. J. 1997; 53: 351­-368.
• 7. Tortuero F.: Influence of implantation of Lactobacillus acidophilus in chicks on the growth, feed conversion, malabsorbtion of fats syndrome and intestinal flora. Poult. Sci. 1973; 52: 197­-203.
• 8. Roura E., Homedes J., Klasing K.C.: Prevention of immunologic stress contributes to the growth­permitting ability of dietary antibiotics in chicks. J. Nutr. 1992; 122: 12, 2383-­2390.
• 9 Omega Diagnostics.: Micropath Antigens/Febrile Antigen Kit. Omega Diagnostics Ltd, Scotland, UK.
• 10. Xie H., Rath N.C., Huff G.R., Huff W.E., Balog J.M.: Effects of Salmonella typhimurium lipopolysaccharide on broiler chickens. Poult. Sci. 2000; 79: 1, 33­-40.
• 11. Koh T.S., Peng R.K., Klasing K.C.: Dietary copper level affects copper metabolism during lipopolysaccharide induced immunological stress in chicks. Poult. Sci. 1996 Jul.; 75 (7): 867-­872.
• 12. Schimizu M., Fitzsimmons R.C., Nakai S.: Anti­E. coli immunoglobulin Y isolated from egg yolk of immunized chickens as a potential food ingredient. J. Food Sci. 1988; 53: 1360­1366.
• 13. Sunwoo H.H., Nakano T., Dixon W.T, Sim J.S.: Immune responses in chickens against lipopolysaccharide ofEscherichia coli and Salmonella typhimurium. Poult. Sci. 1996; 75: 342-­345.