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Determination of the Parasites in the Faeces of Laboratory Animals by Using Flotation Technique in Istanbul, Turkey

Year 2017, Volume: 1 Issue: 2, 35 - 39, 18.08.2017
https://doi.org/10.30704/http-www-jivs-net.322964

Abstract

The aim of this study was to determine the parasites of laboratory and pet animals in Istanbul, Turkey. A total of 128 cages including 279 animals as 115 rabbits from 82 cages, 75 mice from 5 cages, 28 rats from 7 cages, 26 guinea pigs from 23 cages and 35 hamsters from 11 cages were used. Faecal samples were obtained from 8 commercial pet shops in 6 different districts of Istanbul and 2 laboratory animal suppliers. All samples were examined by flotation technique using saturated salt solution. Eimeria spp. oocysts were found in the faeces of rabbits, hamsters and mice, and the prevalence of the infections were 29.6%, 28.6% and 20%, respectively. Two of 115 rabbits were infected with Passalurus ambiguus (1.7%). Trichurid eggs were determined in 2 of 35 hamsters (5.7%). Mice were infected with Syphacia spp., Aspiculuris spp. and the infection ratios were 20% and 40% respectively. Out of 28 rats, 20 were infected with only Syphacia spp. (71.4%). No parasites were observed in guinea pigs’ faeces. Laboratory animals were used not only as experimental animals but also as pets. Therefore coprological examinations for parasite eggs and oocysts are important for treatment and control of the infections in these animals and for protecting the human health because of zoonotic potential of some species.



 

References

  • Baker, D. G. (1998). Natural pathogens of laboratory mice, rats, and rabbits and their effects on research. Clinical Microbiology Reviews, 11, 231-266. Beyhan, Y. E., Gürler, A. T., Bölükbaş, C. S., Açıcı, M. & Umur, Ş. (2010). Bazı laboratuvar hayvanlarında nekropsi ve dışkı bakısı ile saptanan helmintler. Türkiye Parazitol Dergisi 34(2), 98-101.
  • Beyhan, Y. E., Özkan, A. T. & İde, T. (2013). Laboratuvar fare, sıçan ve kobaylarında dışkı bakısı ile helmintlerin araştırılması. Etlik Veteriner Mikrobiyoloji Dergisi, 24, 33-36.
  • Bıyıkoğlu, G. (1996). Bazı laboratuvar hayvanlarında dışkı bakılarında saptanan helmintler. Etlik Veteriner Mikrobiyoloji Dergisi, 8 (4), 137-146. Buluş, F. & Öge, H. (1999). Değişik kurumlardaki tavşanlarda (Oryctolagus cuniculus) dışkı bakısına göre saptanan helmintler. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 46 (2-3), 309-312.
  • Burgu, A., Doğanay, A. & Yılmaz, H. (1986). Laboratuvar beyaz fare ve ratlarında Syphacia obvelata ve S.muris enfeksiyonları. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 33 (3), 434-451.
  • Chen, X. M., Li, X., Lin, R. Q., Deng, J. Y., Fan, W. Y., Yuan, Z. G., Liao, M. & Zhu X. Q. (2011). Pinworm infection in laboratory mice in southern China. Laboratory Animals, 45, 58-60.
  • Dammann, P., Hilken, G., Hueber, B., Köhl, W., Bappert, M. T. & Mahler, M. (2011). Infectious microorganisms in mice (Mus musculus) purchased from commercial pet shops in Germany. Laboratory Animals, 45, 271-275.
  • Gilioli, R., Andrade, L. A. G., Passos, L. A. C., Silva, F. A., Rodrigues, D. M. & Guaraldo, A. M. A. (2000). Parasite Survey in mouse and rat colonies of Brazilian laboratory animal houses kept under different sanitary barrier conditions. Arquivo Brasileiro de Medicina Veterinária e Zootecnia (Brazilian Journal of Veterinary and Animal Science), 52, 1327-1334.
  • Göksu, K., Alibaşoğlu, M. & Dinçer, Ş. (1972). Beyaz fareler (Mus musculus var. albinos) ve beyaz kemelerde (Rattus norvegicus var. albinos) helminthiasis'ler. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 117- 126.
  • Griffiths, H. J. (1971). Some common parasites of small laboratory animals. Laboratory Animals, 5, 123-135.
  • Gudissa, T., Mazengia, H., Alemu, S. & Nigussie, H. (2011). Prevalence of gastrointestinal parasites of laboratory animals at Ethiopian Health and Nutrition Research Institute (EHNRI), Addis Ababa. Journal of Infectious Diseases and Immunity, 3(1), 1-5.
  • Gürler, A. T. & Doğanay, A. (2007). Ankara ve civarında bulunan tavşanlarda solunum ve sindirim sistemi helmintlerinin yaygınlığı. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 54, 105-109.
  • Hendrix, C., M. (2006). Diagnostic Veterinary Parasitology. 2nd ed. St louis, USA, Mosby Inc. ISBN: 9780815185444
  • Hsu, C. K. (1980). Parasitic diseases: how to monitor them and their effects on research. Laboratory Animals, 14, 48-53.
  • Kaufmann, J. (1996). Parasite infection of domestic animals. A diagnostic manual. Basel, Switzerland, Birkhause Verlag.
  • Kılınçel, Ö., Öztürk, C. E., Gün, E., Öksüz, Ş., Uzun, H., Şahin, İ. & Kılıç, N. (2015). A Rare case of hymenolepis diminuta Infection in a small child. Mikrobiyoloji Bülteni, 49(1),135- 138. Lv, C. C., Feng, C., Qi, M., Yang, H. Y., Jian, F. C., Ning, C. S. & Zhang, L. X. (2009). Investigation on the prevalence of gastrointestinal parasites in pet hamsters. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi, 27, 279-80.
  • Medeiros, V. B. (2012). Endo and ectoparasites in conventionally maintained rodents laboratory animals. Journal of Surgical Research 3(1), 27-40.
  • Motamedi, G., Moharami, M., Paykari, H., Eslampanah, M. & Omraninava, A. (2014). A Survey on the gastrointestinal parasites of rabbit and guinea pig in a laboratory animal house. Archives of Razi Institute, 69 (1), 77-81.
  • National Research Council (1991). Infectious diseases of mice and rats: a report of the institute of laboratory animal resources committee on infectious diseases of mice and rats. Washington, D.C. USA, National Academy Press.
  • Pam, V. A., Golu, M., Igeh, C. P. & Ashi, R. D. (2013). Parasitic infections of some laboratory animals in Vom, Plateau State. Journal of Veterinary Advances, 3(2), 87-91.
  • Sürsal, N., Gökpinar, S. & Yildiz, K. (2014). Prevalence of intestinal parasites in hamsters and rabbits in some pet shops of Turkey. Türkiye Parazitoloji Dergisi, 38: 102-105.
  • Şenlik, B., Diker, A. İ. & Küçükyıldız, F. (2005). Bazı laboratuvar hayvanlarında dışkı muayenesi ile saptanan helmintler. Türkiye Parazitoloji Dergisi, 29, 123-125.
  • Tanideh, N., Sadjjadi, S. M., Mohammadzadeh, T. & Mehrabani, D. (2010). Helminthic infections of laboratory animals in animal house of shiraz university of medical sciences and the potential risks of zoonotic infections for researchers. transgenic. Iranian Red Crescent Medical Journal, 12(2), 151-157.
  • Yazar, S., Hamamcı, B., Ünver, A. C. & Şahin, I. (2002). Ratlarda bağırsak parazitlerinin araştırılması. Türkiye Parazitoloji Dergisi,, 26, 212-213.

Determination of the Parasites in the Faeces of Laboratory Animals by Using Flotation Technique in Istanbul, Turkey

Year 2017, Volume: 1 Issue: 2, 35 - 39, 18.08.2017
https://doi.org/10.30704/http-www-jivs-net.322964

Abstract

The aim of this study was to determine the parasites of laboratory and pet animals in Istanbul, Turkey. A total of 128 cages including 279 animals as 115 rabbits from 82 cages, 75 mice from 5 cages, 28 rats from 7 cages, 26 guinea pigs from 23 cages and 35 hamsters from 11 cages were used. Faecal samples were obtained from 8 commercial pet shops in 6 different districts of Istanbul and 2 laboratory animal suppliers. All samples were examined by flotation technique using saturated salt solution. Eimeria spp. oocysts were found in the faeces of rabbits, hamsters and mice, and the prevalence of the infections were 29.6%, 28.6% and 20%, respectively. Two of 115 rabbits were infected with Passalurus ambiguus (1.7%). Trichurid eggs were determined in 2 of 35 hamsters (5.7%). Mice were infected with Syphacia spp., Aspiculuris spp. and the infection ratios were 20% and 40% respectively. Out of 28 rats, 20 were infected with only Syphacia spp. (71.4%). No parasites were observed in guinea pigs’ faeces. Laboratory animals were used not only as experimental animals but also as pets. Therefore coprological examinations for parasite eggs and oocysts are important for treatment and control of the infections in these animals and for protecting the human health because of zoonotic potential of some species.

 

References

  • Baker, D. G. (1998). Natural pathogens of laboratory mice, rats, and rabbits and their effects on research. Clinical Microbiology Reviews, 11, 231-266. Beyhan, Y. E., Gürler, A. T., Bölükbaş, C. S., Açıcı, M. & Umur, Ş. (2010). Bazı laboratuvar hayvanlarında nekropsi ve dışkı bakısı ile saptanan helmintler. Türkiye Parazitol Dergisi 34(2), 98-101.
  • Beyhan, Y. E., Özkan, A. T. & İde, T. (2013). Laboratuvar fare, sıçan ve kobaylarında dışkı bakısı ile helmintlerin araştırılması. Etlik Veteriner Mikrobiyoloji Dergisi, 24, 33-36.
  • Bıyıkoğlu, G. (1996). Bazı laboratuvar hayvanlarında dışkı bakılarında saptanan helmintler. Etlik Veteriner Mikrobiyoloji Dergisi, 8 (4), 137-146. Buluş, F. & Öge, H. (1999). Değişik kurumlardaki tavşanlarda (Oryctolagus cuniculus) dışkı bakısına göre saptanan helmintler. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 46 (2-3), 309-312.
  • Burgu, A., Doğanay, A. & Yılmaz, H. (1986). Laboratuvar beyaz fare ve ratlarında Syphacia obvelata ve S.muris enfeksiyonları. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 33 (3), 434-451.
  • Chen, X. M., Li, X., Lin, R. Q., Deng, J. Y., Fan, W. Y., Yuan, Z. G., Liao, M. & Zhu X. Q. (2011). Pinworm infection in laboratory mice in southern China. Laboratory Animals, 45, 58-60.
  • Dammann, P., Hilken, G., Hueber, B., Köhl, W., Bappert, M. T. & Mahler, M. (2011). Infectious microorganisms in mice (Mus musculus) purchased from commercial pet shops in Germany. Laboratory Animals, 45, 271-275.
  • Gilioli, R., Andrade, L. A. G., Passos, L. A. C., Silva, F. A., Rodrigues, D. M. & Guaraldo, A. M. A. (2000). Parasite Survey in mouse and rat colonies of Brazilian laboratory animal houses kept under different sanitary barrier conditions. Arquivo Brasileiro de Medicina Veterinária e Zootecnia (Brazilian Journal of Veterinary and Animal Science), 52, 1327-1334.
  • Göksu, K., Alibaşoğlu, M. & Dinçer, Ş. (1972). Beyaz fareler (Mus musculus var. albinos) ve beyaz kemelerde (Rattus norvegicus var. albinos) helminthiasis'ler. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 117- 126.
  • Griffiths, H. J. (1971). Some common parasites of small laboratory animals. Laboratory Animals, 5, 123-135.
  • Gudissa, T., Mazengia, H., Alemu, S. & Nigussie, H. (2011). Prevalence of gastrointestinal parasites of laboratory animals at Ethiopian Health and Nutrition Research Institute (EHNRI), Addis Ababa. Journal of Infectious Diseases and Immunity, 3(1), 1-5.
  • Gürler, A. T. & Doğanay, A. (2007). Ankara ve civarında bulunan tavşanlarda solunum ve sindirim sistemi helmintlerinin yaygınlığı. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 54, 105-109.
  • Hendrix, C., M. (2006). Diagnostic Veterinary Parasitology. 2nd ed. St louis, USA, Mosby Inc. ISBN: 9780815185444
  • Hsu, C. K. (1980). Parasitic diseases: how to monitor them and their effects on research. Laboratory Animals, 14, 48-53.
  • Kaufmann, J. (1996). Parasite infection of domestic animals. A diagnostic manual. Basel, Switzerland, Birkhause Verlag.
  • Kılınçel, Ö., Öztürk, C. E., Gün, E., Öksüz, Ş., Uzun, H., Şahin, İ. & Kılıç, N. (2015). A Rare case of hymenolepis diminuta Infection in a small child. Mikrobiyoloji Bülteni, 49(1),135- 138. Lv, C. C., Feng, C., Qi, M., Yang, H. Y., Jian, F. C., Ning, C. S. & Zhang, L. X. (2009). Investigation on the prevalence of gastrointestinal parasites in pet hamsters. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi, 27, 279-80.
  • Medeiros, V. B. (2012). Endo and ectoparasites in conventionally maintained rodents laboratory animals. Journal of Surgical Research 3(1), 27-40.
  • Motamedi, G., Moharami, M., Paykari, H., Eslampanah, M. & Omraninava, A. (2014). A Survey on the gastrointestinal parasites of rabbit and guinea pig in a laboratory animal house. Archives of Razi Institute, 69 (1), 77-81.
  • National Research Council (1991). Infectious diseases of mice and rats: a report of the institute of laboratory animal resources committee on infectious diseases of mice and rats. Washington, D.C. USA, National Academy Press.
  • Pam, V. A., Golu, M., Igeh, C. P. & Ashi, R. D. (2013). Parasitic infections of some laboratory animals in Vom, Plateau State. Journal of Veterinary Advances, 3(2), 87-91.
  • Sürsal, N., Gökpinar, S. & Yildiz, K. (2014). Prevalence of intestinal parasites in hamsters and rabbits in some pet shops of Turkey. Türkiye Parazitoloji Dergisi, 38: 102-105.
  • Şenlik, B., Diker, A. İ. & Küçükyıldız, F. (2005). Bazı laboratuvar hayvanlarında dışkı muayenesi ile saptanan helmintler. Türkiye Parazitoloji Dergisi, 29, 123-125.
  • Tanideh, N., Sadjjadi, S. M., Mohammadzadeh, T. & Mehrabani, D. (2010). Helminthic infections of laboratory animals in animal house of shiraz university of medical sciences and the potential risks of zoonotic infections for researchers. transgenic. Iranian Red Crescent Medical Journal, 12(2), 151-157.
  • Yazar, S., Hamamcı, B., Ünver, A. C. & Şahin, I. (2002). Ratlarda bağırsak parazitlerinin araştırılması. Türkiye Parazitoloji Dergisi,, 26, 212-213.
There are 23 citations in total.

Details

Subjects Veterinary Surgery
Journal Section Research Articles
Authors

Handan Çetinkaya

Tuğba Taş This is me

Cem Vuruşaner

Publication Date August 18, 2017
Published in Issue Year 2017 Volume: 1 Issue: 2

Cite

APA Çetinkaya, H., Taş, T., & Vuruşaner, C. (2017). Determination of the Parasites in the Faeces of Laboratory Animals by Using Flotation Technique in Istanbul, Turkey. Journal of Istanbul Veterinary Sciences, 1(2), 35-39. https://doi.org/10.30704/http-www-jivs-net.322964

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