Year 2020, Volume 4 , Issue 1, Pages 13 - 20 2020-04-30

Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors
Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors

Emin KARAKURT [1] , Mushap KURU [2] , Serpil DAĞ [3] , Enver BEYTUT [4] , Hilmi NUHOĞLU [5] , Murat Can DEMİR [6] , Cihan KAÇAR [7] , Özhan KARATAŞ [8] , Ayfer YILDIZ [9]


In this study, we aimed to evaluate the proliferative, metastatic and apoptotic capacities of TVT cases, which are in various phases of development, by using immunohistochemical markers. The material of this study consisted of twelve female and six male dogs diagnosed with TVT brought to our department between 2007 and 2020 years. Diff-quick staining was applied to the smear taken from tumoral masses for cytological examinations. Tumoral tissues from dogs were fixed in a 10% neutral buffered formaldehyde solution. After routine tissue procedures Hematoxylin & Eosin stain was applied to the sections. Tissue sections were investigated under a light microscope and photographed. Immunohistochemical staining was performed on the tissues using the avidin-biotin immune peroxidase complex method. As a result of macroscopic, cytological and histopathological examinations, TVT positive cases were divided into three according to their developmental stages. While the expression of PCNA, MMP-9, mutant p53 and Bcl-2 increased significantly in progressive cases compared to regressive and stable cases, Bax expression increased significantly in regressive cases compared to progressive and stable cases. In conclusion, we thought that the mentioned markers are very useful for understanding the prognosis of TVT, the tumor aggressiveness and the survival of the malignant cells.

In this study, we aimed to evaluate the proliferative, metastatic and apoptotic capacities of TVT cases, which are in various phases of development, by using immunohistochemical markers. The material of this study consisted of twelve female and six male dogs diagnosed with TVT brought to our department between 2007 and 2020 years. Diff-quick staining was applied to the smear taken from tumoral masses for cytological examinations. Tumoral tissues from dogs were fixed in a 10% neutral buffered formaldehyde solution. After routine tissue procedures Hematoxylin & Eosin stain was applied to the sections. Tissue sections were investigated under a light microscope and photographed. Immunohistochemical staining was performed on the tissues using the avidin-biotin immune peroxidase complex method. As a result of macroscopic, cytological and histopathological examinations, TVT positive cases were divided into three according to their developmental stages. While the expression of PCNA, MMP-9, mutant p53 and Bcl-2 increased significantly in progressive cases compared to regressive and stable cases, Bax expression increased significantly in regressive cases compared to progressive and stable cases. In conclusion, we thought that the mentioned markers are very useful for understanding the prognosis of TVT, the tumor aggressiveness and the survival of the malignant cells. 

  • Referans1Akkoc, A., Nak, D., Demirer, A., Şimşek, G. (2017). Immunocharacterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 in canine transmissible venereal tumors. Biotechnic & Histochemistry, 92 (2), 100-106.
  • Referans2Alzate, J. M., Montoya-Florez, L. M., Pérez, J.E., Rocha, N. S., Pedraza-Ordonez, F. J. (2019). The role of the multi-drug resistance 1, p53, b cell lymphoma 2, and bcl 2-associated X genes in the biologic behavior and chemotherapeutic resistance of canine transmissible venereal tumors. Veterinary Clinical Pathology, 48 (4), 730-739.
  • Referans3Aresu, L., Giantin, M., Morello, E., Vascellari, M., Castagnaro, M., Lopparelli, R., Zancanella, V., Granato, A., Garbisa, S., Aricò, A., Bradaschia, A., Mutinelli, F., Dacasto, M. (2011). Matrix metalloproteinases and their inhibitors in canine mammary tumors. BMC Veterinary Research, 7, 33.
  • Referans4Chu, R. M., Lin, C. Y., Liu, C. C., Yang, S. Y., Hsiao, Y. W., Hung, S. W., Pao, H. N., Liao, K.W. (2001). Proliferation characteristics of canine transmissible venereal tumor. Anticancer Research. 21 (6A), 4017-4024.
  • Referans5Coskan, A.S., Alcigir, M. E., Atalay Vural, S. (2011). Pathomorphological and immunohistochemical findings in a case of extragenital canine transmissible venereal tumour. Bulgarian Journal of Veterinary Medicine, 14 (4), 252-256.
  • Referans6Flórez, M.M., Fêo, H. B., da Silva, G. N., Yamatogi, R. S., Aguiar, A. J., Araújo Jr, J. P., Rocha, N. S. (2017). Cell cycle kinetics, apoptosis rates and gene expressions of MDR-1, TP53, BCL-2 and BAX in transmissible venereal tumour cells and their association with therapy response. Veterinary and Comparative Oncology, 15 (3), 793-807
  • Referans7Gerardi, D. G., Tinucci-Costa, M. Silveira, A. C. T., Moro, J. V. (2014). Expression of P-glycoprotein, multidrug resistance-associated protein, glutathione-S-transferase pi and p53 in canine transmissible venereal tumor. Pesquisa Veterinária Brasileira, 34 (1), 71-78.
  • Referans8Gupta, K. & Sood, N. K. (2012). Pathological and immunohistochemical studies on rare cases of primary extra-genital transmissible venereal tumours in the mammary gland. Veterinarni Medicina, 57 (4), 198-206.
  • Referans9Guvenc, T., Haligur, M., Orman, M.N., Haziroglu, R. (2002). Mitosis and apoptosis in canine cutaneous histiocytoma and transmissible venereal tumour. Acta Veterinaria Hungarica, 50 (3), 315-321.
  • Referans10Lima, C. R. O., Faleiro, M. B. R., Rabelo, R. E., Vulcani, V. A. S., Rubini, M. R., Torres, F. A. G., Moura, V. M. B. D. (2016). Insertion of the LINE-1 element in the C-MYC gene and immunoreactivity of C-MYC, p53, p21 and p27 proteins in different morphological patterns of the canine TVT. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 68 (3), 658-666.
  • Referans11Lokesh, J. V., Kurade, N. P., Shivakumar, M. U., Sharma, A. K., Maiti, S. K. Evaluation of BcL-2 and PCNA expression and mitotic index in spontaneous canine tumors. Advances in Animal and Veterinary Sciences, 2 (1), 63-66.
  • Referans12Loukopoulos, P., Mungall, B. A., Straw, R. C., Thornton, J. R., Robinson, W. F. (2003). Matrix metalloproteinase-2 and -9 involvement in canine tumors. Veterinary Pathology, 40 (4), 382-394.
  • Referans13Madewell, B. R. (2001). Cellular proliferation in tumors: a review of methods, interpretation, and clinical applications. Journal of Veterinary Internal Medicine, 15 (4), 334-340.
  • Referans14Moro, J. V., Tinucci-Costa, M., Silveira, A. C. T., Gerardi, D. G., Alessi, A. C. (2010). Reactivity of p53 protein in canine transmissible venereal tumor. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 62 (2), 318-323.
  • Referans15Oguş, E. & Özmen, Ö. (2018). Immunohistochemical determination of the matrix metalloproteinase-2 and -7 expression in transmissible venereal tumor in dogs. Mehmet Akif Ersoy Üniversitesi Veteriner Fakültesi Dergisi, 3(2), 106-110.
  • Referans16Özalp, G. R., Zik, B., Bastan, A., Peker, S., Özdemir-Salci, E. S., Bastan, I., Darbaz, I., Salar, S., Karakas, K. (2012). Vincristine modulates the expression of Ki67 and apoptosis in naturally occurring canine transmissible venereal tumor (TVT). Biotechnic & Histochemistry, 87 (5), 325-330.
  • Referans17Özyiğit, M. Ö., Nak, D, Akşit, H., İnan Öztürkoğlu, S. Şimşek, G. Uzabacı, E., Nak. Y, Seyrek, K. (2014). The effects of vincristine sulfate on expression of galectin-3, Bcl-2, and carbohydrate structures specific for EEL, GSL-1, and RCA-1 lectins in bitches with naturally occuring canine transmissible venereal tumor. Turkish Journal of Veterinary and Animal Sciences, 38, 331-338.
  • Referans18Paramjeet, Jangir, B. L., Lather, D., Saharan, S. Jakhar, K. K. (2019). Pathomorphological and immunohistochemical studies on transmissible venereal tumours in dogs. Haryana Veterinarian, 58 (1), 82-85.
  • Referans19Santos, F. G. A., Vasconcelos, A. C., Nunes, J. E. S., Cassali, G. D., Paixão, T. A., Martins, A. S., Silva, S. S., Martins, R. F., Moro, L. (2008). Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases. Arquivo Brasileiro de Medicina Veterinária e Zootecnia,60 (3), 607-612.
  • Referans20Setthawongsin, C., Teewasutrakul, P., Tangkawattana, S., Techangamsuwan, S., Rungsipipat, A. (2019). Conventional-vincristine sulfate vs. modified protocol of vincristine sulfate and l-asparaginase in canine transmissible venereal tumor. Frontiers in Veterinary Science, 6, 300.
  • Referans21Stockmann, D., Ferrari, H. F., Andrade, A. L., Rodrigo, A. L., Cardoso, T. C., Luvizotto, M. C. (2011a). Canine Transmissible Venereal Tumors: Aspects Related to Programmed Cell Death. Brazilian Journal of Veterinary Pathology, 4 (1), 67-75.
  • Referans22Stockmann, D., Ferrari, H. F., Andrade, A. L., Cardoso, T. C., Luvizotto, M. C. (2011b). Detection of the tumour suppressor gene TP53 and expression of p53, Bcl-2 and p63 proteins in canine transmissible venereal tumour. Veterinary and Comparative Oncology, 9 (4), 251-259.
  • Referans23Tiwari, K. P., Bhaiyat, M.I., Chikweto, A., Inga, A., Sharma, R. N., Pawaiya, R.V.S. (2014). Immunohistochemical detection of p53 tumor suppressor protein in round cell tumors of dogs in Grenada, West Indies. Advances in Animal and Veterinary Sciences, 2 (3S), 23-27.
  • Referans24Tiwari, K.P., Bhaiyat, M.I., Chikweto, A., Sharma, R.N., Younger, C.H., Pawaiya, R.V.S. (2016). Expression pattern of proliferating cell nuclear antigen (PCNA) in round cell tumors of dogs from Grenada, West Indies. Indian Journal of Veterinary Pathology, 40 (2), 144-147.
Primary Language en
Subjects Veterinary
Journal Section Research Articles
Authors

Author: Emin KARAKURT (Primary Author)
Institution: KAFKAS ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0003-4409-251X
Author: Mushap KURU
Institution: KAFKAS ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0001-7667-689X
Author: Serpil DAĞ
Institution: KAFKAS ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0003-3360-2940
Author: Enver BEYTUT
Institution: KAFKAS UNIVERSITY
Country: Turkey


Orcid: 0000-0003-2530-2542
Author: Hilmi NUHOĞLU
Institution: KAFKAS ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-3803-069X
Author: Murat Can DEMİR
Institution: KAFKAS ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-2642-697X
Author: Cihan KAÇAR
Institution: KAFKAS ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-2778-8059
Author: Özhan KARATAŞ
Institution: Cumhuriyet Üniversitesi
Country: Turkey


Orcid: 0000-0002-6569-5435
Author: Ayfer YILDIZ
Institution: Kafkas Üniversitesi Sağlık Bilimleri Enstitüsü
Country: Turkey


Dates

Publication Date : April 30, 2020

Bibtex @research article { http-www-jivs-net700692, journal = {Journal of Istanbul Veterinary Sciences}, issn = {2602-3490}, eissn = {2602-3490}, address = {}, publisher = {The Chamber of Veterinary Surgeons, Istanbul}, year = {2020}, volume = {4}, pages = {13 - 20}, doi = {10.30704/http-www-jivs-net.700692}, title = {Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors}, key = {cite}, author = {Karakurt, Emin and Kuru, Mushap and Dağ, Serpil and Beytut, Enver and Nuhoğlu, Hilmi and Demi̇r, Murat Can and Kaçar, Cihan and Karataş, Özhan and Yıldız, Ayfer} }
APA Karakurt, E , Kuru, M , Dağ, S , Beytut, E , Nuhoğlu, H , Demi̇r, M , Kaçar, C , Karataş, Ö , Yıldız, A . (2020). Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors . Journal of Istanbul Veterinary Sciences , 4 (1) , 13-20 . DOI: 10.30704/http-www-jivs-net.700692
MLA Karakurt, E , Kuru, M , Dağ, S , Beytut, E , Nuhoğlu, H , Demi̇r, M , Kaçar, C , Karataş, Ö , Yıldız, A . "Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors" . Journal of Istanbul Veterinary Sciences 4 (2020 ): 13-20 <https://dergipark.org.tr/en/pub/http-www-jivs-net/issue/53542/700692>
Chicago Karakurt, E , Kuru, M , Dağ, S , Beytut, E , Nuhoğlu, H , Demi̇r, M , Kaçar, C , Karataş, Ö , Yıldız, A . "Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors". Journal of Istanbul Veterinary Sciences 4 (2020 ): 13-20
RIS TY - JOUR T1 - Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors AU - Emin Karakurt , Mushap Kuru , Serpil Dağ , Enver Beytut , Hilmi Nuhoğlu , Murat Can Demi̇r , Cihan Kaçar , Özhan Karataş , Ayfer Yıldız Y1 - 2020 PY - 2020 N1 - doi: 10.30704/http-www-jivs-net.700692 DO - 10.30704/http-www-jivs-net.700692 T2 - Journal of Istanbul Veterinary Sciences JF - Journal JO - JOR SP - 13 EP - 20 VL - 4 IS - 1 SN - 2602-3490-2602-3490 M3 - doi: 10.30704/http-www-jivs-net.700692 UR - https://doi.org/10.30704/http-www-jivs-net.700692 Y2 - 2020 ER -
EndNote %0 Journal of Istanbul Veterinary Sciences Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors %A Emin Karakurt , Mushap Kuru , Serpil Dağ , Enver Beytut , Hilmi Nuhoğlu , Murat Can Demi̇r , Cihan Kaçar , Özhan Karataş , Ayfer Yıldız %T Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors %D 2020 %J Journal of Istanbul Veterinary Sciences %P 2602-3490-2602-3490 %V 4 %N 1 %R doi: 10.30704/http-www-jivs-net.700692 %U 10.30704/http-www-jivs-net.700692
ISNAD Karakurt, Emin , Kuru, Mushap , Dağ, Serpil , Beytut, Enver , Nuhoğlu, Hilmi , Demi̇r, Murat Can , Kaçar, Cihan , Karataş, Özhan , Yıldız, Ayfer . "Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors". Journal of Istanbul Veterinary Sciences 4 / 1 (April 2020): 13-20 . https://doi.org/10.30704/http-www-jivs-net.700692
AMA Karakurt E , Kuru M , Dağ S , Beytut E , Nuhoğlu H , Demi̇r M , Kaçar C , Karataş Ö , Yıldız A . Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors. J Ist Vet Sci. 2020; 4(1): 13-20.
Vancouver Karakurt E , Kuru M , Dağ S , Beytut E , Nuhoğlu H , Demi̇r M , Kaçar C , Karataş Ö , Yıldız A . Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors. Journal of Istanbul Veterinary Sciences. 2020; 4(1): 13-20.