This study aims to determine the possible relationship between the levels of Kallistatin and the severity of liver injury in rats that were exposed to carbon tetrachloride (CCl4). According to the results, serum ALT, AST, LDH, GGT and Collagen-I and Collagen-III levels in rats in the severe group were higher than control. Histopathological examination of rats in injury groups showed severe morphological changes that were resulted in cell dissociation and disruption of the liver lobe architecture in the liver parenchyma of rats that received CCl4. Kallistatin serum level decreasing respectively in M1, M2, and S groups compared to the control group, and the lowest amount was belonging to the severe group. As a result, there was a reverse connection between Kallistatin serum level and the liver injury intensity. Serum kallistatin levels are an essential parameter in determining liver tissue damage levels, and measuring it may help provide a treatment prognosis.