Determination of estrogen receptor, progesterone receptor and epidermal growth factor receptor in canine vaginal tumors and vaginal fold prolapse: A preliminary study

Year 2022, Volume: 6 Issue: 1, 35 - 41, 30.04.2022

Zeynep Günay Uçmak , Atila Ateş , Özge Erdoğan Bamaç , Hazal Öztürk Gürgen , İsmail Kırşan , Çağatay Tek , Çağla Nur Küçükbekir , İbrahim Kurban

https://doi.org/10.30704/http-www-jivs-net.1066060

Cited By: 1

Abstract

Eight dogs with a vaginal mass were incorporated into the study. According to the vaginal examination (vaginoscopy, exfoliative vaginal cytology) and histopathology, the groups were designed as vaginal fold prolapse (Group VFP; n=3) and vaginal tumor (Group VT; n=5). Vaginal tissue samples were homogenized. Tissue homogenates were analyzed with enzyme-linked immunosorbent assay (ELISA) to determine the levels of estrogen receptor (ER), progesterone receptor (PR), and epidermal growth factor receptor (EGFR). The mean ER level was significantly higher in group VT compared to the group VFP (P<0.05). However, PR and EGFR levels did not showed significance related to the groups (P>0.05). In conclusion, significant differences were obtained between the bitches with VT and VFP related to ER levels although both groups have pathological conditions. Further studies are needed to discuss these pathologies with healthy bitches in terms of steroid receptor and EGFR levels in both vaginal tissue and blood sera.

Keywords

Canine, leiomyoma, leiomyosarcoma, vaginal fold prolapse, receptor

Thanks

The authors are also grateful to Prof. Dr. Bülent Ekiz from the Department of Animal Breeding and Husbandry for his help with the statistical analysis.

References

• Alan, M., Çetin, Y., Sendag, S.,& Eski, F. (2007). True Vaginal Prolapse in a Bitch. Animal Reproduction Science, 100, 411–414.
• Anila, B., Bibin, B. B., Jayakumar, C., Shibu, S., Indu V. R., & Kurien, M.O. (2020). Occurrence of vaginal hyperplasia among intact dogs. Journal of Veterinary and Animal Sciences, 51(2), 142-145.
• Anya, K.O., Oguejiofor, C.F., Nnaji, T.O., & Udeani, I.J. (2020) Vaginal Hyperplasia and Progressive Vaginal Fold Prolapse in a Bullmastiff Bitch. Open Journal of Veterinary Medicine, 10, 55-63.
• Baba, A.I., & Câtoi, C. (2007). Female Genital Tract Tumors. In: A.I. Baba & C. Câtoi (Eds.) Comparative Oncology. Bucharest (RO): The Publishing House of the Romanian Academy. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK9559/
• Brodey, R. S., & Roszel, J. F. (1967). Neoplasms of the canine uterus, vagina, and vulva: a clinicopathologic survey of 90 cases. Journal of the American Veterinary Medical Association, 151(10), 1294–1307.
• Brunner, A., Grimm, C., Polterauer, S., Hefler, L., Stani, J., Dudek, G., & Horvat, R. (2011). Expression of epidermal growth factor receptor and vascular endothelial growth factor in vaginal squamous cell cancer. American Journal of Obstetrics and Gynecology, 204(2), 171.e1–171.e1716.
• Cotchin, E. (1954). Neoplasia in the dog. Veterinary Record, 66, 879-884.
• Cooper, B.J., & Valentine, B.A. (2016). Tumors of muscle. In: Meuten D.J. (Ed.). Tumors in domestic animals 5th ed. (pp. 425–446). John Wiley & Sons, Inc.
• Enginler, S.Ö., Siğirci, U., Arun, S.S., & Ekici, H. (2014). Vaginal leiomyosarcoma subsequent to pyometra in a Labrador Retriever bitch. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 40(1), 109-113.
• Ferré-Dolcet, L., Romagnoli, S., Banzato, T., Cavicchioli, L., Di Maggio, R., Cattai, A., Berlanda, M., Schrank, M., & Mollo, A. (2020). Progesterone-responsive vaginal leiomyoma and hyperprogesteronemia due to ovarian luteoma in an older bitch. BMC Veterinary Research, 16(1), 284.
• Greenberg, D., & Yates, D. (2002). What is your diagnosis? Journal of Small Animal Practice, 43(9), 381- 406.
• Iguchi, T., Edery, M., Tasi, P. S., Ozawa, S., Sato, T., & Bern, H. A. (1993). Epidermal growth factor receptor levels in reproductive organs of female mice exposed neonatally to diethylstilbestrol. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine, 204(1), 110–116.
• Ithurralde, J., Costas, A. L., Pessina, P., Cueto, E., Fila, D., & Meikle, A. (2013). Immunohistochemical determination of estrogen receptor-α in canine vaginal biopsies throughout proestrus, estrus, and early diestrus. Theriogenology, 80(7), 805–811.
• Klein, M. K. (2001). Tumors of the female reproductive system. In: S.J. Withrow & E.G. MacEwen (Eds.). Small Animal Clinical Oncology, (pp. 445-454), W.B. Saunders, Philadelphia.
• Manothaiudom, K., & Johnston, S. D. (1991). Clinical approach to vaginal/vestibular masses in the bitch. The Veterinary Clinics of North America Small Animal Practice, 21(3), 509–521.
• McEntee, M.C. (2002). Reproductive oncology. Clinical Techniques in Small Animal Practice, 17(3), 133–149.
• Millán, Y., Gordon, A., de los Monteros, A. E., Reymundo, C., & de las Mulas, J. M. (2007). Steroid receptors in canine and human female genital tract tumours with smooth muscle differentiation. Journal of comparative pathology, 136(2-3), 197–201.
• Mukku, V. R., & Stancel, G. M. (1985). Regulation of epidermal growth factor receptor by estrogen. Journal of Biological Chemistry, 260, 9820–9824.
• Ohta, Y., Sato, T., & Iguchi, T. (1993). Immunocytochemical localization of progesterone receptor in the reproductive tract of adult female rats. Biology of Reproduction, 48(1), 205–213.
• Rollón, E., Millán, Y.,& de las Mulas, J.M. (2008). Effects of aglepristone, a progesterone receptor antagonist, in a dog with a vaginal fibroma. Journal of Small Animal Practice, 49(1), 41–3.
• Root Kustritz, M. V. (2006). Collection of tissue and culture samples from the canine reproductive tract. Theriogenology, 66(3), 567–574.
• Sağsöz, H., Liman, N., Güney Saruhan, B., Akbal K, M. E., Ketani, M. A., & Topalo Lu, U. U. (2019). Expression and localisation of epidermal growth factor receptors and their ligands in the lower genital tract of cycling cows. Reproduction, Fertility, and Development, 31(11), 1692–1706.
• Sathya, S., & Linn, K. (2014). Regression of a vaginal leiomyoma after Ovariohysterectomy in a dog: a case report. Journal of the American Animal Hospital Association, 50(6), 424–428.
• Schutte, A.P. (1967). Vaginal Prolapse in the Bitch. Journal of the South African Veterinary Association, 38, 197-203.
• Sontas, H.B., Ekici, H., & Romagnoli, S. (2010) Canine Vaginal Fold Prolapse: A Comprehensive Literature Review. European Journal of Companion Animal Practice, 20, 127-135.
• Thacher, C., & Bradley, R. L. (1983). Vulvar and vaginal tumors in the dog: a retrospective study. Journal of the American Veterinary Medical Association, 183(6), 690–692.
• Uchima, F. D., Edery, M., Iguchi, T., Larson, L., & Bern, H. A. (1987). Growth of mouse vaginal epithelial cells in culture: functional integrity of the estrogen receptor system and failure of estrogen to induce proliferation. Cancer letters, 35(3), 227–235.
• Vermeirsch, H.,Van den Broeck,W., &Simoens, P. (2002). Immunolocalization of sex steroid hormone receptors in canine vaginal and vulvar tissue and their relation to sex steroid hormone levels. Reproduction, Fertility and Development, 14, 251-258.
• Zhao, Y., Li, Y., & Xu, Y. (2003). Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences, 35(1), 37–40.