Silicone plastination of spinal cord of cat: as an alternative specimen for neuroanatomy education

Year 2022, Volume: 6 Issue: 2, 65 - 69, 31.08.2022

Okan Ekim , Hasen Awel Yunus , Caner Bakıcı , Barış Batur

https://doi.org/10.30704/http-www-jivs-net.1096113

Abstract

Plastination is a technique that aims to preserve biological materials for education, training, and research. Plastinated models increase knowledge and skill, make students easily understand the complex anatomical parts of the central nervous system, meanwhile can reduce the use of animals in research and education. The study aimed to produce a silicone plastinated model of the spinal cord of a cat for practical teaching of neuroanatomy. The spinal cord of a stray cat that died of natural causes was plastinated using silicone plastination method. The cervical spinal nerves (1-8) and brachial plexus were demonstrated. The thoracic region of the spinal cord was also well preserved, but the demonstration of thoracic spinal nerves became very difficult because of too much thinness of the nerves. The lumbosacral plexus was preserved well. In this region cranial iliohypogastric nerve, caudal iliohypogastric nerve, ilioinguinal nerve, femoral nerve, gluteal nerve, ischiadic nerve, obturator nerve, pudendal nerve and cauda equina were visible. The spinal cord of cats prepared by silicone plastination methods can be used as an alternative sample to formalin preserved specimens.

Keywords

Cat, Neuroanatomy, Silicone Plastination, Spinal Cord

References

• Baroni, M., & Heinold, Y. (1995). A review of the clinical diagnosis of feline infectious peritonitis viral meningo encephalomyelitis. Progress in veterinary neurology, 3, 88–94.
• Basset, A. A. E., Seleem, A. A., & Mohamed, S.K. (2014). Silicon plastination of brachial and lumbosacral plexuses and cauda equina in goat; educational Neuroanatomical Studies. Basrah journal of veterinary research, 1(2):193-212.
• Bican, O., Minagar, A., & Pruitt, A. A. (2013). The spinal cord: a review of functional neuroanatomy. Neurologic Clinics, 31, 1-18.
• Forterre, F., Tomek, A., Konar, M., Vandevelde, M., Howard, J., & Jaggy, A. (2007). Multiple meningiomas: clinical, radiological, surgical, and pathological findings with outcome in four cats. Journal of Feline Medicine and Surgery, 9, 36–43.
• Garosi, L. (2004). The neurological examination. In: Platt S, Olby N, eds. BSAVA manual of canine and feline neurology. 3rd ed. Gloucester: BSAVA, Pp1–23.
• Henry, R. W., & Latorre R. (2007). Polyester plastination of biological tissue: P40 technique for brain slices. Journal of Plastination, 22, 59-68.
• Henry, R. W., Larry, J., & Carol, H. (1997). Specimen Preparation for Silicone Plastination. Journal of International Society for Plastination, 12, 13-17.
• Henry, R. W., Van Hagens, G., & Seamans, G. (2019). Cold temperature/Biodur® /S10/von Hagens' Silicone plastination technique. Anatomia Histologia Embryologia, 48, 532–538.
• Heslnga, F. J. M., & Delerkauf, F. A. (1992). Action of formaldehyde solution on human brain lipids. https://journals.sagepub.com/doi/pdf/10.1177/10.6.704https://journals.sagepub.com/doi/pdf/10.1177/10.6.704.
• Holladay, S. D., & Hudson L. C. (1989). Use of plastinated brains in teaching neuroanatomy at the North Carolina state university, college of veterinary medicine. Department of Anatomy, Physiological Sciences, and Radiology. Journal of International Society for Plastination, 3(1), 15-17.
• Jain, M., Kasetty, S., & Sudheendra, U. S. (2017). Plastination: An intricate and real display of oral hard and soft tissues specimens. Journal of Dental Research, 1, 1-6. DOI: 10.5171/2014.639870.
• König H. E. & Liebich, H. G. (2020). Veterinary anatomy of domestic mammals. Textbook and Colour Atlas. 7th ed. New York, US: Thieme.
• Knipe, M. F., Vernau K, M., Hornof, W. J., & Lecouteur, R. (2001). Intervertebral disc extrusion in six cats. Journal of Feline Medicine and Surgery, 3, 161–168.
• Lahunta, A. D., Glass, E., & Kent, M. (2008). Veterinary Neuroanatomy and Clinical Neurology. 3rd Edition, Sounders US. Pp15-40 ISBN: 9780721667065.
• Legendre, A. M., & Whitenack, D. L. (1995). Feline infectious peritonitis with spinal cord involvement in two cats. Journal of the American Veterinary Medical Association, 167, 931–932.
• Lu, D., Lamb, C. R., Wesselingh, K., & Targett, M. P. (2002). Acute intervertebral disc extrusion in a cat and MRI findings. Journal of Feline Medicine and Surgery, 4, 65–68.
• Marioni-Henry, K., Charles, H. V., Alisa, L. N., & Thomas, J. V. W. (2004). Prevalence of Diseases of the Spinal Cord of Cats. Journal of Veterinary Internal Medicine, 18, 851–858.
• Marioni-Henry, K., Van Winkle, T. J., Smith, S. H., & Vite C. H. (2008). Tumors affecting the spinal cord of cats: 85 cases (1980–2005). Journal of the American Veterinary Medical Association, 232, 237–243.
• Munana, K. R., Olby, N. J., Sharp, N. J. H, & Skeen, T. M. (2001). Intervertebral disc disease in 10 cats. Journal of the American Animal Hospital Association, 37, 384-389.
• NAV, (Nomina Anatomica Veterinaria) (2017). International Committee on Veterinary Gross Anatomical
• Nomenclature (ICVGAN). Published by the Editorial Committee.
• Pashaei, S. (2010). A brief review on the history, methods and applications of plastination. Journal of Morphology, 28, 1075-1079.
• Robert, W. H., Larry, J., & Carol, H. (1997). Specimen Preparation for Silicone Plastination. Journal of International Society for Plastination, 12, 13-17.
• Robert, W. H., Van Hagens, G., & Seamans, G. (2019). Cold temperature/Biodur® /S10/von Hagens' Silicone plastination technique. Anatomia Histologia Embryologia, 48, 532–538.
• Schoefert, A. K. (2019). Comparative Neuropathology (1962): Attending to Neuropathologies across multiple species. Journal of the History of Medicine and Allied Sciences, 74(2), 192–215.
• Sivrev, D. P., Hamza, S. R., Dimitrov, N. D., & Georgieva, A. I. (2013). Using of P40 technique for brain sheet plastination. Faculty of Medicine, University of Thrace, St.Zagora,Bulgaria.UDC57611.http://journals.nubip.edu.ua/index.php/Veterenarna/article/view/4202.
• Tomalty, D., Pang, S. C., & Ellis, R. E. (2019). Preservation of neural tissue with a formaldehyde‐free phenol‐based embalming protocol. Clinical Anatomy, 32, 224-230.
• Toossi, A., Bergin, B., Marefatallah, M., Parhizi, B., Tyreman, N., Dirk., Everaert, G., Rezaei, S., Peter, S, ChristopherGatenby, J., Steve, I. P., & Mushahwar, K. V. (2021). Comparative neuroanatomy of the lumbosacral spinal cord of the rat, cat, pig, monkey, and human. Scientific Reports, 11, 1955-1919.
• Vail, D. M., & Macewen, E. G. (2000). Spontaneously occurring tumors of companion animals as models for human cancer. Cancer Investigation. 18(8), 781-792.
• Von Hagens, G., Tiedemann, K., & Kriz, W. (1987). The current potential of plastination. Anatomy and Embryology,175, 411-421.