Determination of Salmonella spp. prevalence and antibiotic resistance profiles in domestic animals

Year 2023, Vetexpo-2022, 153 - 153, 05.01.2023

Merve Yıldız Serpil Kahya Demirbilek

Abstract

Besides being a foodborne zoonotic pathogen, Salmonella can be a potential source for humans due to close contact between pets and their owners. This study aimed to prevalence and antimicrobial resistance of Salmonella spp. in apparently healthy and diarrheic cats and dogs. In addition, macroscopic appearances of lactic acid bacteria(LAB) isolated from feces of both Salmonella positive and Salmonella negative dogs were investigated. As a result of the bacteriological examination of rectal swab samples taken from a total of 341 pets, 184 cats and 157 dogs, brought to private clinics, Salmonella spp. was positive in 9 (5.73%) healthy-looking dogs, while Salmonella spp. was not seen in cats (0.00%). As a result of macroscopic examination of LAB, there was no significant difference between Salmonella-positive dogs and negative ones. In addition, there was a relationship between the occurrence of Salmonella spp. in dogs and the consumption of raw meat and the living environment. When the antibiotic resistance profiles were examined in 9 isolates, resistance formation was observed against 6 of the 19 antibiotics tested according to their MIC values, while the resistance rates were respectively: CIP (21.05%), SXT, LVX, AM (10.52%), ETP, SXA (5, 26 %). And when 13 antibiotics were analyzed for 9 isolates using the disk diffusion method, resistance was observed against 6 of them and the resistance rates were found as NA (15.38%), TE, DO, C, FFC, S3 (7.69%). Multi Drug Resistance (MDR) was observed in 2 of 9 positive isolates and when a preliminary screen for Extended-Spectrum-B-Lactamase (ESBL) production was performed, ESBL was not detected for any isolate. As a result, dogs were seen as Salmonella ssp.carriers, and when Salmonella carriers were examined in healthy dogs with diarrhea, Salmonella was isolated in healthy dogs. It has been determined that hygiene practices should be observed after contact with dog feces and that dogs should be fed well-cooked foods to reduce the risk of Salmonella spp.

Keywords

Salmonella, cat, dog, antimicrobial sensitivity, Lactobacillus