Avian IgY antibodies and its immunotherapeutic applications

Year 2024, Volume: 8 Issue: 1, 64 - 74, 30.04.2024

Kürşat Tetik

https://doi.org/10.30704/http-www-jivs-net.1443841

Abstract

Antibodies, also called immunoglobulins, are specialized proteins produced by the immune system in response to the presence of pathogens or foreign substances in the body. These unique proteins are commonly used for diagnostic and therapeutic purposes because they easily bind to antigenic molecules. Polyclonal antibody production currently involves the use of laboratory animals such as rats, rabbits, sheep, goats, and horses. However, the manufacture of these antibodies generally involves practices that cause pain to animals, such as prolonged bloodletting. In recent years, isolating antibodies from egg yolk following hyperimmunization of chickens has emerged as a popular approach for producing significant amounts of antibodies. This approach combines the principles of natural passive immunity and artificial passive immunity. To ensure a continuous accumulation of antibodies in egg yolks, chickens are regularly immunized with specific antigens. Egg yolk antibodies, known as IgY, are extracted and used for immunotherapy and immunodiagnostic purposes in human and animal applications due to their promising antibacterial properties. The antibacterial properties of egg yolk antibodies have been a significant focus in IgY studies. Several reports have shown that IgY helps prevent bacterial transmission or infection in vivo. The production of IgY against mammalian antigens has a higher success rate than IgG production. This is because of the phylogenetic difference between mammals and chickens. Furthermore, these antibodies have a more comprehensive range of antigenic epitope recognition and can respond to more than one species, making them more versatile. This study compiles information on the properties, mechanisms of action, and uses of egg yolk antibodies based on existing literature on IgY technology.

Keywords

IgY, egg yolk antibodies, passive immunization, poultry

References

• Aizenshtein, E., Yosipovich, R., Kvint, M., Shadmon, R., Krispel, S., Shuster, E., Shahar, E. (2016). Practical aspects in the use of passive immunization as an alternative to attenuated viral vaccines. Vaccine, 34(22), 2513-2518.
• Araújo, A., Lobato, Z., Chávez-Olórtegui, C., & Velarde, D. (2010). Brazilian IgY-Bothrops antivenom: Studies on the development of a process in chicken egg yolk. Toxicon, 55(4), 739-744.
• Berghman, L., Abi-Ghanem, D., Waghela, S., & Ricke, S. (2005). Antibodies: an alternative for antibiotics? Poultry Science, 84(4), 660-666.
• Bollen, L. S., Crowley, A., Stodulski, G., & Hau, J. (1996). Antibody production in rabbits and chickens immunized with human IgG A comparison of titre and avidity development in rabbit serum, chicken serum and egg yolk using three different adjuvants. Journal of Immunological Methods, 191(2), 113-120.
• Carlander, D., Kollberg, H., Wejåker, P.-E., & Larsson, A. (2000). Peroral immunotheraphy with yolk antibodies for the prevention and treatment of enteric infections. Immunologic Research, 21, 1-6.
• Chalghoumi, R., Beckers, Y., Portetelle, D., & Théwis, A. (2009). Hen egg yolk antibodies (IgY), production and use for passive immunization against bacterial enteric infections in chicken: a review. Biotechnologie, Agronomie, Société et Environnement, 13(3). Chansarkar, N. (1998). Studies on structural stability of hen’s egg yolk immunoglobulin (IgY). PhD thesis: University of British Columbia, Vancouver, Canada.
• Coleman, M. (1999). Using egg antibodies to treat diseases. Egg Nutrition and Biotechnology., 351-370.
• Fellah, J. S., Kerfourn, F., Wiles, M. V., Schwager, J., & Charlemagne, J. (1993). Phylogeny of immunoglobulin heavy chain isotypes: structure of the constant region of Ambystoma mexicanum υ chain deduced from cDNA sequence. Immunogenetics, 38, 311-317.
• Fink, A. L., Williams, K. L., Harris, E., Alvine, T. D., Henderson, T., Schiltz, J., Bradley, D. S. (2017). Dengue virus specific IgY provides protection following lethal dengue virus challenge and is neutralizing in the absence of inducing antibody dependent enhancement. Plos Neglected Tropical Diseases, 11(7), e0005721.
• Gadde, U., Rathinam, T., & Lillehoj, H. S. (2015). Passive immunization with hyperimmune egg-yolk IgY as prophylaxis and therapy for poultry diseases-a review. Animal Health Research Reviews, 16(2), 163.
• Gao, X., Zhang, X., Lin, L., Yao, D., Sun, J., Du, X., Zhang, Y. (2016). Passive immune- protection of Litopenaeus vannamei against Vibrio harveyi and Vibrio parahaemolyticus infections with anti-Vibrio egg yolk (IgY)-encapsulated feed. International Journal of Molecular Sciences, 17(5), 723.
• Gassmann, M., Thömmes, P., Weiser, T., & Hübscher, U. (1990). Efficient production of chicken egg yolk antibodies against a conserved mammalian protein. The FASEB Journal, 4(8), 2528-2532.
• Girard, F., Batisson, I., Martinez, G., Breton, C., Harel, J., & Fairbrother, J. M. (2006). Use of virulence factor-specific egg yolk-derived immunoglobulins as a promising alternative to antibiotics for prevention of attaching and effacing Escherichia coli infections. FEMS Immunology & Medical Microbiology, 46(3), 340-350.
• Gottstein, B., & Hemmeler, E. (1985). Egg yolk immunoglobulin Y as an alternative antibody in the serology of Echinococcosis. Zeitschrift für Parasitenkunde, 71(2), 273-276.
• Grzywa, R., Łupicka-Słowik, A., Walczak, M., Idzi, M., Bobrek, K., Boivin, S., Sieńczyk, M. (2014). Highly sensitive detection of cancer antigen 15-3 using novel avian IgY antibodies. ALTEX-Alternatives to Animal Experimentation, 31(1), 43-52.
• Hatta, H., Tsuda, K., Akachi, S., Kim, M., Yamamoto, T., & Ebina, T. (1993). Oral passive immunization effect of anti-human Rotavirus IgY and its behavior against proteolytic enzymes. Bioscience, Biotechnology, and Biochemistry, 57(7), 1077-1081.
• Ikemori, Y., Kuroki, M., Peralta, R., Yokoyama, H., & Kodama, Y. (1992). Protection of neonatal calves against fatal enteric colibacillosis by administration of egg yolk powder from hens immunized with K99-piliated enterotoxigenic Escherichia coli. American Journal of Veterinary Research, 53(11), 2005-2008.
• Jaradat, Z. W., & Marquardt, R. R. (2000). Studies on the stability of chicken IgY in different sugars, complex carbohydrates and food materials. Food and Agricultural Immunology, 12(4), 263-272.
• Jin, L., Baidoo, S. K., Marquardt, R. R., & Frohlich, A. A. (1998). In vitro inhibition of adhesion of enterotoxigenic Escherichia coli K88 to piglet intestinal mucus by egg-yolk antibodies. FEMS Immunology & Medical Microbiology, 21(4), 313-321.
• Karlsson, M., Kollberg, H., & Larsson, A. (2004). Chicken IgY: utilizing the evolutionary advantage. World's Poultry Science Journal, 60(3), 341-348.
• Klemperer, F. (1893). Ueber natürliche Immunität und ihre Verwerthung für die Immunisirungstherapie. Archiv Für Experimentelle Pathologie und Pharmakologie, 31(4- 5), 356-382.
• Larsson, A., Bålöw, R.-M., Lındahl, T. L., & Forsberg, P.-O. (1993). Chicken antibodies: taking advantage of evolution-a review. Poultry Science, 72(10), 1807-1812.
• Larsson, A., Wejåker, P.-E., Forsberg, P.-O., & Lindahl, T. (1992). Chicken antibodies: a tool to avoid interference by complement activation in ELISA. Journal of Immunological Methods, 156(1), 79-83.
• Lee, K., Chang, S.-K., Lee, Y.-J., Lee, J.-H., & Koo, N.-S. (2002). Acid Stability of Anti- Helicobacter pyroli IgY in in Aqueous Polyol Solution. BMB Reports, 35(5), 488-493.
• Lee, W., Atif, A. S., Tan, S. C., & Leow, C. H. (2017). Insights into the chicken IgY with emphasis on the generation and applications of chicken recombinant monoclonal antibodies. Journal of Immunological Methods, 447, 71-85.
• Leslie, G. A., & Clem, L. (1969). Phylogeny of immunoglobulin structure and function: III. Immunoglobulins of the chicken. The Journal of Experimental Medicine, 130(6), 1337-1352.
• Lösch, U., Schranner, I., Wanke, R., & Jürgens, L. (1986). The chicken egg, an antibody source. Journal of Veterinary Medicine, Series B, 33(1‐10), 609-619.
• Tupicka-Słowik, A., Walczak, M., Grzywa, R., Bobrek, K., Łęcka, M., Boivin, S., Sieńczyk, M. (2014). Generation and application of polyclonal IgY antibodies specific for full- length and nicked prostate-specific antigen. Bioanalysis, 6(23), 3197-3213.
• Magor, K. E., Higgins, D. A., Middleton, D. L., & Warr, G. W. (1994). One gene encodes the heavy chains for three different forms of IgY in the duck. Journal of Immunology (Baltimore, Md.: 1950), 153(12), 5549-5555.
• Malekshahi, Z. V., Gargari, S. L. M., Rasooli, I., & Ebrahimizadeh, W. (2011). Treatment of Helicobacter pylori infection in mice with oral administration of egg yolk-driven anti-UreC immunoglobulin. Microbial Pathogenesis, 51(5), 366-372.
• Meenatchisundaram, S., Shanmugam, V., & Anjali, V. (2011). Development of chicken egg yolk antibodies against Streptococcus mitis–purification and neutralizing efficacy. Journal of Basic and Clinical Pharmacy, 2(2), 109. Mendoza, J. C., Vivas, D., Rodríguez, E., Inga, R., Sandoval, G., Lazo, F., & Yarlequé, A. (2012). Eficacia experimental de anticuerpos IgY producidos en huevos, contra el veneno de la serpiente peruana
• Bothrops atrox. Revista Peruana de Medicina Experimental y Salud Pública, 29, 69-75. Michael, A., Meenatchisundaram, S., Parameswari, G., Subbraj, T., Selvakumaran, R., & Ramalingam, S. (2010). Chicken egg yolk antibodies (IgY) as an alternative to mammalian antibodies. Indian J. Sci. Technol, 3(4), 468-474.
• Mine, Y., & Kovacs-Nolan, J. (2002). Chicken egg yolk antibodies as therapeutics in enteric infectious disease: a review. Journal of Medicinal Food, 5(3), 159-169.
• Najdi, S., Brujeni, G. N., Sheikhi, N., & Chakhkar, S. (2016). Development of anti- Helicobacter pylori immunoglobulins Y (IgYs) in quail. Iranian Journal of Veterinary Research, 17(2), 106.
• Nguyen, H. H., Tumpey, T. M., Park, H.-J., Byun, Y.-H., Tran, L. D., Nguyen, V. D., Seong, B. L. (2010). Prophylactic and therapeutic efficacy of avian antibodies against Influenza virus H5N1 and H1N1 in mice. PLoS One, 5(4), e10152.
• Olbrich, C., Müller, R. H., Tabatt, K., Kayser, O., Schulze, C., & Schade, R. (2002). Stable biocompatible adjuvants—a new type of adjuvant based on solid lipid nanoparticles: a study on cytotoxicity, compatibility and efficacy in chicken. Alternatives to Laboratory Animals, 30(4), 443-458.
• Otani, H., Matsumoto, K., Saeki, A., & Hosono, A. (1991). Comparative studies on properties of hen egg yolk IgY and rabbit serum IgG antibodies. Lebensmittel-Wissenschaft+ Technologie, 24(2), 152-158.
• Parvari, R., Avivi, A., Lentner, F., Ziv, E., Tel‐Or, S., Burstein, Y., & Schechter, I. (1988). Chicken immunoglobulin gamma‐heavy chains: limited VH gene repertoire, combinatorial diversification by D gene segments and evolution of the heavy chain locus. The EMBO Journal, 7(3), 739-744.
• Peralta, R., Yokoyama, H., Ikemori, Y., Kuroki, M., & Kodama, Y. (1994). Passive immunisation against experimental Salmonellosis in mice by orally administered hen egg- yolk antibodies specific for 14-kDa fimbriae of Salmonella Enteritidis. Journal of Medical Microbiology, 41(1), 29-35.
• Pereira, E., Van Tilburg, M., Florean, E., & Guedes, M. (2019). Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review. International Immunopharmacology, 73, 293-303.
• Pizarro-Guajardo, M., Díaz-González, F., Álvarez-Lobos, M., & Paredes-Sabja, D. (2017). Characterization of chicken IgY specific to Clostridium difficile R20291 spores and the effect of oral administration in mouse models of initiation and recurrent disease. Frontiers in Cellular and Infection Microbiology, 7, 365.
• Polson, A. v., von Wechmar, M. B., & Fazakerley, G. (1980). Antibodies to proteins from yolk of immunized hens. Immunological Communications, 9(5), 495-514.
• Rahman, S., Van Nguyen, S., Icatlo Jr, F. C., Umeda, K., & Kodama, Y. (2013). Oral passive IgY-based immunotherapeutics: a novel solution for prevention and treatment of alimentary tract diseases. Human Vaccines & Immunotherapeutics, 9(5), 1039-1048.
• Reddy, P. K., Shekar, A., Kingston, J. J., Sripathy, M. H., & Batra, H. (2013). Evaluation of IgY capture ELISA for sensitive detection of Alpha hemolysin of Staphylococcus aureus without staphylococcal protein A interference. Journal of Immunological Methods, 391(1-2), 31-38.
• Reilly, R. M., Domingo, R., & Sandhu, J. (1997). Oral delivery of antibodies: future pharmacokinetic trends. Clinical Pharmacokinetics, 32, 313-323.
• Revathy, J., Karthika, S., Sentila, R., & Michael, A. (2014). In vitro evaluation of the efficacy of chicken egg yolk antibodies (IgY) generated against Propionibacterium acnes. International Journal of Cosmetic Science, 36(1), 68-73.
• Russell, W. M. S., & Burch, R. L. (1959). The principles of humane experimental technique. London, England: Methuen. Retriwed from: https://norecopa.no/textbase/the-principles-of-humane-experimental-technique
• Schade, R., Behn, I., Erhard, M., Hlinak, A., & Staak, C. (2000). Chicken egg yolk antibodies, production and application: IgY-Technology. Springer Science & Business Media. DOI: 10.1007/978-3-662-04488-9
• Schade, R., Calzado, E. G., Sarmiento, R., Chacana, P. A., Porankiewicz-Asplund, J., & Terzolo, H. R. (2005). Chicken egg yolk antibodies (IgY-technology): a review of progress in production and use in research and human and veterinary medicine. Alternatives to Laboratory Animals, 33(2), 129-154.
• Schade, R., & Hlinak, A. (1996). Egg yolk antibodies, state of the art and future prospects. ALTEX-Alternatives to Animal Experimentation, 13(Supp1), 5-9.
• Schmidt, P., Wiedemann, V., Kühlmann, R., Wanke, R., Linckh, E., & Lösch, U. (1989). Chicken Egg Antibodies for Prophylaxis and Therapy of Infectious Intestinal Diseases: II. In vitro studies on gastric and enteric digestion of egg yolk antibodies specific against pathogenic Escherichia coli strains. Journal of Veterinary Medicine, Series B, 36(1‐10), 619-628.
• Shi, H., Zhu, J., Zou, B., Shi, L., Du, L., Long, Y., Sun, L. (2017). Effects of specific egg yolk immunoglobulin on pan-drug-resistant Acinetobacter baumannii. Biomedicine & Pharmacotherapy, 95, 1734-1742.
• Shimizu, M., Fitzsimmons, R. c., & Nakai, S. (1988). Anti‐E. coli lmmunoglobulin Y isolated from egg yolk of immunized chickens as a potential food ingredient. Journal of Food Science, 53(5), 1360-1368.
• Shimizu, M., Nagashima, H., & Hashimoto, K. (1993). Comparative studies in molecular stability of immunoglobulin G from different species. Comparative Biochemistry and Physiology. B, Comparative biochemistry, 106(2), 255-261.
• Shimizu, M., Nagashima, H., Hashimoto, K., & Suzuki, T. (1994). Egg yolk antibody (Ig Y) stability in aqueous solution with high sugar concentrations. Journal of Food Science, 59(4), 763-765.
• Shimizu, M., Nagashima, H., Sano, K., Hashimoto, K., Ozeki, M., Tsuda, K., & Hatta, H. (1992). Molecular stability of chicken and rabbit immunoglobulin G. Bioscience, Biotechnology, and Biochemistry, 56(2), 270-274.
• Sudjarwo, S. A., Eraiko, K., & Sudjarwo, G. W. (2017). The activity of immunoglobulin Y anti-Mycobacterium tuberculosis on proliferation and cytokine expression of rat peripheral blood mononuclear cells. Pharmacognosy Research, 9(Suppl 1), S5.
• Sugita-Konishi, Y., Ogawa, M., Arai, S., Kumagai, S., Igimi, S., & Shimizu, M. (2000). Blockade of Salmonella Enteritidis passage across the basolateral barriers of human intestinal epithelial cells by specific antibody. Microbiology and Immunology, 44(6), 473-479.
• Sugita-Konishi, Y., Shibata, K., Yun, S. S., Hara-Kudo, Y., Yamaguchi, K., & Kumagai, S. (1996). Immune functions of immunoglobulin Y isolated from egg yolk of hens immunized with various infectious bacteria. Bioscience, Biotechnology, and Biochemistry, 60(5), 886-888.
• Sui, J., Cao, L., & Lin, H. (2011). Antibacterial activity of egg yolk antibody (IgY) against Listeria monocytogenes and preliminary evaluation of its potential for food preservation. Journal of the Science of Food and Agriculture, 91(11), 1946-1950.
• Sunwoo, H., Lee, E., Menninen, K., Suresh, M., & Sim, J. (2002). Growth inhibitory effect of chicken egg yolk antibody (IgY) on Escherichia coli O157: H7. Journal of Food Science, 67(4), 1486-1494.
• Thibodeau, A., Fravalo, P., Perron, A., Lewandowski, S. L., & Letellier, A. (2017). Production and characterization of anti-Campylobacter jejuni IgY derived from egg yolks. Acta Veterinaria Scandinavica, 59(1), 1-9.
• Thomsen, K., Christophersen, L., Jensen, P. Ø., Bjarnsholt, T., Moser, C., & Høiby, N. (2016). Anti-Pseudomonas aeruginosa IgY antibodies promote bacterial opsonization and augment the phagocytic activity of polymorphonuclear neutrophils. Human Vaccines & Immunotherapeutics, 12(7), 1690-1699.
• Tini, M., Jewell, U., Camenisch, G., Chilov, D., & Gassmann, M. (2002). Generation and application of chicken egg-yolk antibodies. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 131(3), 569-574.
• Tsubokura, K., Berndtson, E., Bogstedt, A., Kaijser, B., Kim, M., Ozeki, M., & Hammarström, L. (1997). Oral administration of antibodies as prophylaxis and therapy in Campylobacter jejuni‐infected chickens. Clinical & Experimental Immunology, 108(3), 451-455.
• Wang, L.-H., Li, X.-Y., Jin, L.-J., You, J.-S., Zhou, Y., Li, S.-Y., & Xu, Y.-P. (2011). Characterization of chicken egg yolk immunoglobulins (IgYs) specific for the most prevalent capsular serotypes of mastitis-causing Staphylococcus aureus. Veterinary Microbiology, 149(3-4), 415-421.
• Wanke, R., Schmidt, P., Erhard, M., Stangassinger, M., Schmahl, W., & Hermanns, W. (1996). Freund's complete adjuvant in the chicken: efficient immunostimulation with severe local inflammatory reaction. Zentralblatt fur Veterinarmedizin. Reihe A, 43(4), 243-253.
• Warr, G. W., Magor, K. E., & Higgins, D. A. (1995). IgY: clues to the origins of modern antibodies. Immunology Today, 16(8), 392-398.
• Wen, J., Zhao, S., He, D., Yang, Y., Li, Y., & Zhu, S. (2012). Preparation and characterization of egg yolk immunoglobulin Y specific to Influenza B virus. Antiviral Research, 93(1), 154-159.
• Xu, Y., Li, X., Jin, L., Zhen, Y., Lu, Y., Li, S., Wang, L. (2011). Application of chicken egg yolk immunoglobulins in the control of terrestrial and aquatic animal diseases: a review. Biotechnology Advances, 29(6), 860-868.
• Yokoyama, H., Peralta, R. C., Diaz, R., Sendo, S., Ikemori, Y., & Kodama, Y. (1992). Passive protective effect of chicken egg yolk immunoglobulins against experimental enterotoxigenic Escherichia coli infection in neonatal piglets. Infection and Immunity, 60(3), 998-1007.
• Yokoyama, H., Umeda, K., Peralta, R. C., Hashi, T., Icatlo Jr, F. C., Kuroki, M., Kodama, Y. (1998). Oral passive immunization against experimental Salmonellosis in mice using chicken egg yolk antibodies specific for Salmonella Enteritidis and S. Typhimurium. Vaccine, 16(4), 388-393.
• Yolken, R. H., Leister, F., Wee, S.-B., Miskuff, R., & Vonderfecht, S. (1988). Antibodies to rotaviruses in chickens' eggs: a potential source of antiviral immunoglobulins suitable for human consumption. Pediatrics, 81(2), 291-295.
• Zhang, X.-Y., Vieira-Pires, R. S., Morgan, P. M., & Schade, R. (2021). IgY-Technology: Production and Application of Egg Yolk Antibodies. In: Springer. DOI: 0.1007/978-3- 030-72688-1.
• Schade, R., & Hlinak, A. (1996). Egg yolk antibodies, state of the art and future prospects. ALTEX-Alternatives to Animal Experimentation, 13(Supp1), 5-9.
• Schmidt, P., Wiedemann, V., Kühlmann, R., Wanke, R., Linckh, E., & Lösch, U. (1989). Chicken Egg Antibodies for Prophylaxis and Therapy of Infectious Intestinal Diseases: II. In vitro studies on gastric and enteric digestion of egg yolk antibodies specific against pathogenic Escherichia coli strains. Journal of Veterinary Medicine, Series B, 36(1‐10), 619-628.
• Shi, H., Zhu, J., Zou, B., Shi, L., Du, L., Long, Y., Sun, L. (2017). Effects of specific egg yolk immunoglobulin on pan-drug-resistant Acinetobacter baumannii. Biomedicine & Pharmacotherapy, 95, 1734-1742.
• Shimizu, M., Fitzsimmons, R. c., & Nakai, S. (1988). Anti‐E. coli lmmunoglobulin Y isolated from egg yolk of immunized chickens as a potential food ingredient. Journal of Food Science, 53(5), 1360-1368.
• Shimizu, M., Nagashima, H., & Hashimoto, K. (1993). Comparative studies in molecular stability of immunoglobulin G from different species. Comparative Biochemistry and Physiology. B, Comparative biochemistry, 106(2), 255-261.
• Shimizu, M., Nagashima, H., Hashimoto, K., & Suzuki, T. (1994). Egg yolk antibody (Ig Y) stability in aqueous solution with high sugar concentrations. Journal of Food Science, 59(4), 763-765.
• Shimizu, M., Nagashima, H., Sano, K., Hashimoto, K., Ozeki, M., Tsuda, K., & Hatta, H. (1992). Molecular stability of chicken and rabbit immunoglobulin G. Bioscience, Biotechnology, and Biochemistry, 56(2), 270-274.
• Sudjarwo, S. A., Eraiko, K., & Sudjarwo, G. W. (2017). The activity of immunoglobulin Y anti-Mycobacterium tuberculosis on proliferation and cytokine expression of rat peripheral blood mononuclear cells. Pharmacognosy Research, 9(Suppl 1), S5.
• Sugita-Konishi, Y., Ogawa, M., Arai, S., Kumagai, S., Igimi, S., & Shimizu, M. (2000). Blockade of Salmonella Enteritidis passage across the basolateral barriers of human intestinal epithelial cells by specific antibody. Microbiology and Immunology, 44(6), 473-479.
• Sugita-Konishi, Y., Shibata, K., Yun, S. S., Hara-Kudo, Y., Yamaguchi, K., & Kumagai, S. (1996). Immune functions of immunoglobulin Y isolated from egg yolk of hens immunized with various infectious bacteria. Bioscience, Biotechnology, and Biochemistry, 60(5), 886-888.
• Sui, J., Cao, L., & Lin, H. (2011). Antibacterial activity of egg yolk antibody (IgY) against Listeria monocytogenes and preliminary evaluation of its potential for food preservation. Journal of the Science of Food and Agriculture, 91(11), 1946-1950.
• Sunwoo, H., Lee, E., Menninen, K., Suresh, M., & Sim, J. (2002). Growth inhibitory effect of chicken egg yolk antibody (IgY) on Escherichia coli O157: H7. Journal of Food Science, 67(4), 1486-1494.
• Thibodeau, A., Fravalo, P., Perron, A., Lewandowski, S. L., & Letellier, A. (2017). Production and characterization of anti-Campylobacter jejuni IgY derived from egg yolks. Acta Veterinaria Scandinavica, 59(1), 1-9.
• Thomsen, K., Christophersen, L., Jensen, P. Ø., Bjarnsholt, T., Moser, C., & Høiby, N. (2016). Anti-Pseudomonas aeruginosa IgY antibodies promote bacterial opsonization and augment the phagocytic activity of polymorphonuclear neutrophils. Human Vaccines & Immunotherapeutics, 12(7), 1690-1699.
• Tini, M., Jewell, U., Camenisch, G., Chilov, D., & Gassmann, M. (2002). Generation and application of chicken egg-yolk antibodies. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 131(3), 569-574.
• Tsubokura, K., Berndtson, E., Bogstedt, A., Kaijser, B., Kim, M., Ozeki, M., & Hammarström, L. (1997). Oral administration of antibodies as prophylaxis and therapy in Campylobacter jejuni‐infected chickens. Clinical & Experimental Immunology, 108(3), 451-455.
• Wang, L.-H., Li, X.-Y., Jin, L.-J., You, J.-S., Zhou, Y., Li, S.-Y., & Xu, Y.-P. (2011). Characterization of chicken egg yolk immunoglobulins (IgYs) specific for the most prevalent capsular serotypes of mastitis-causing Staphylococcus aureus. Veterinary Microbiology, 149(3-4), 415-421.
• Wanke, R., Schmidt, P., Erhard, M., Stangassinger, M., Schmahl, W., & Hermanns, W. (1996). Freund's complete adjuvant in the chicken: efficient immunostimulation with severe local inflammatory reaction. Zentralblatt fur Veterinarmedizin. Reihe A, 43(4), 243-253.
• Warr, G. W., Magor, K. E., & Higgins, D. A. (1995). IgY: clues to the origins of modern antibodies. Immunology Today, 16(8), 392-398.
• Wen, J., Zhao, S., He, D., Yang, Y., Li, Y., & Zhu, S. (2012). Preparation and characterization of egg yolk immunoglobulin Y specific to Influenza B virus. Antiviral Research, 93(1), 154-159.
• Xu, Y., Li, X., Jin, L., Zhen, Y., Lu, Y., Li, S., Wang, L. (2011). Application of chicken egg yolk immunoglobulins in the control of terrestrial and aquatic animal diseases: a review. Biotechnology Advances, 29(6), 860-868.
• Yokoyama, H., Peralta, R. C., Diaz, R., Sendo, S., Ikemori, Y., & Kodama, Y. (1992). Passive protective effect of chicken egg yolk immunoglobulins against experimental enterotoxigenic Escherichia coli infection in neonatal piglets. Infection and Immunity, 60(3), 998-1007.
• Yokoyama, H., Umeda, K., Peralta, R. C., Hashi, T., Icatlo Jr, F. C., Kuroki, M., Kodama, Y. (1998). Oral passive immunization against experimental Salmonellosis in mice using chicken egg yolk antibodies specific for Salmonella Enteritidis and S. Typhimurium. Vaccine, 16(4), 388-393.
• Yolken, R. H., Leister, F., Wee, S.-B., Miskuff, R., & Vonderfecht, S. (1988). Antibodies to rotaviruses in chickens' eggs: a potential source of antiviral immunoglobulins suitable for human consumption. Pediatrics, 81(2), 291-295.
• Zhang, X.-Y., Vieira-Pires, R. S., Morgan, P. M., & Schade, R. (2021). IgY-Technology: Production and Application of Egg Yolk Antibodies. In: Springer. DOI: 0.1007/978-3- 030-72688-1.
• Xu, Y., Li, X., Jin, L., Zhen, Y., Lu, Y., Li, S., Wang, L. (2011). Application of chicken egg yolk immunoglobulins in the control of terrestrial and aquatic animal diseases: a review. Biotechnology Advances, 29(6), 860-868.
• Yokoyama, H., Peralta, R. C., Diaz, R., Sendo, S., Ikemori, Y., & Kodama, Y. (1992). Passive protective effect of chicken egg yolk immunoglobulins against experimental enterotoxigenic Escherichia coli infection in neonatal piglets. Infection and Immunity, 60(3), 998-1007.
• Yokoyama, H., Umeda, K., Peralta, R. C., Hashi, T., Icatlo Jr, F. C., Kuroki, M., Kodama, Y. (1998). Oral passive immunization against experimental Salmonellosis in mice using chicken egg yolk antibodies specific for Salmonella Enteritidis and S. Typhimurium. Vaccine, 16(4), 388-393.
• Yolken, R. H., Leister, F., Wee, S.-B., Miskuff, R., & Vonderfecht, S. (1988). Antibodies to rotaviruses in chickens' eggs: a potential source of antiviral immunoglobulins suitable for human consumption. Pediatrics, 81(2), 291-295.
• Zhang, X.-Y., Vieira-Pires, R. S., Morgan, P. M., & Schade, R. (2021). IgY-Technology: Production and Application of Egg Yolk Antibodies. In: Springer. DOI: 0.1007/978-3- 030-72688-1.