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Investigation of Chlamydia abortus from aborted ewes in Hakkâri province and its districts by enzyme-linked immunosorbent assay (ELISA)

Year 2025, Volume: 9 Issue: 2, 75 - 87, 31.08.2025

Abstract

aborted during lambing period in different flocks in Hakkari province and it’s district in Türkiye. Blood samples were collected from ewes that had aborted, using the jugular vein in accordance with the technique. The serum obtained from the blood samples collected in biochemical serum tubes was tested for antibodies against Chlamydia abortus using an ELISA test kit (Sunlong sheep Chlamydia abortus ELISA test kit). According to the ELISA test results, 8 samples (4.4%) out of 180 samples collected from Hakkari province and its districts tested positive for C. abortus. The distribution of samples that tested positive for C. abortus according to the ELISA test results was as follows: 4 sheep in the central district of Hakkari, 3 sheep in the district of Yüksekova, and 1 sheep in the district of Şemdinli tested positive for C. abortus. As a result, for the first time, data on the prevalence of C. abortus in sheep farms in Hakkari province center, Yüksekova and Şemdinli districts were obtained. The data obtained were shared with the animal health branch directorates in the agricultural directorates in Hakkari provinces and districts. Especially in the last decade, it will contribute to the development of activities for the development of preventive measures against C. abortus, which is one of the most important factors causing abortion in sheep farms

Project Number

TYL-2023-10809

References

  • Abnaroodheleh, F., Emadi, A., & Dadar, M. (2021). Seroprevalence of brucellosis and chlamydiosis in sheep and goats with history of abortion in Iran. Small Ruminant Research, 202:1-4.
  • Akpınar, R. K., Kılıçoğlu, Y., Şahin, B., Çenesiz, S., Kadı, H., & Çitil, M. (2024). Molecular detection of Chlamydia abortus and investigation of oxidative stress parameters in sheep fetuses in the Black Sea Region of Türkiye. Comparative Clinical Pathology, 33(1), 47-54.
  • Aldama, F. D. J., Montes de Oca Jiménez, R., & Varela Guerrero, J. A. (2022). Diagnóstico, prevención y control de enfermedades causadas por Chlamydia en pequeños rumiantes. Revisión. Revista Mexicana de Ciencias Pecuarias, 13(3), 725-742.
  • Bı̇ngöl, E., & Aygün, T. (2014). Hakkari’de Yetiştirilen Karakaş Koyunlarında Büyüme ve Gelişme Özellikleri. Journal of the Institute of Science and Technology, 4(3), 65-74.
  • Çaya, H., Aslantaş, Ö., İyisan, A. S., Mirioğlu, M., & Tunca, Ş. T. (2006). Chlamydophıla Abortus'a (Chlamydıa Psıttacı Serotype 1) Karşı Oluşan Antikorların Mikrokomplement Fikzasyon (mCFT) Ve Enzyme-Lınked Immunosorbent Assay (ELISA) İle Araştırılması. Etlik Veteriner Mikrobiyoloji Dergisi, 17(1-2), 7-12.
  • Demir, Y., & Tuncer, S. S. (2022). Hakkâri ilindeki küçükbaş hayvancılık işletmelerinin mevcut durumu ve genel sorunları. Van Veterinary Journal, 33(3), 97-105.
  • Esmaeili, H., Bolourchi, M., & Mokhber-Dezfouli, M. R. (2015). Seroprevalence of Chlamydia abortus infection in sheep and goats in Iran. Iranian Journal of Veterinary Medicine, 9(2), 73-77.
  • Esmaeili, H., Shakeri, A. P., Rad, Z. N., Arani, E. B., Villanueva-Saz, S., Ruiz, H., & Lacasta, D. (2022). Causes of abortion in Iranian sheep flocks and associated risk factors. Veterinary Research Communications, 46(4), 1227-1238.
  • Esmaeili, H., Bolourchi, M., Mokhber-Dezfouli, M. R., & Teimourpour, A. (2021). Detection of Chlamydia abortus and risk factors for infection in small ruminants in Iran. Small Ruminant Research, 197, 1-7.
  • Fayez, M., Elmoslemany, A., Alorabi, M., Alkafafy, M., Qasim, I., Al-Marri, T., & Elsohaby, I. (2021). Seroprevalence and risk factors associated with Chlamydia abortus infection in sheep and goats in Eastern Saudi Arabia. Pathogens, 10(4), 489.
  • GAP, (2023, August 18). Uluslararası tarımsal araştırma ve eğitim merkezi. Retrieved from: https://arastirma.tarimorman.gov.tr/gaputaem
  • Giannitti, F., Anderson, M., Miller, M., Rowe, J., Sverlow, K., Vasquez, M., & Cantón, G. (2016). Chlamydia pecorum: fetal and placental lesions in sporadic caprine abortion. Journal of Veterinary Diagnostic Investigation, 28(2), 184-189.
  • Gülaydın, Ö., Öztürk, C., Ekin, İ. H., İlhan, Z., & İlhan, F. (2023). Investigation of selected bacterial agents causing sheep abortion in the Van Province by RT-PCR and histopathological methods. Acta Veterinaria Brno, 92(1), 69-77.
  • Hakkâri il Tarım ve Orman Müdürlüğü, (2024, may 15) Hayvansal üretim. Retrieved from: https://hakkari.tarimorman.gov.tr/Menu/12/ Hakkâri
  • Hireche, S., Agabou, A., & Bounab, B. E. K. (2022). Chlamydia abortus in dairy cattle: Serostatus and risk factors in Algerian northeastern high plateaus. Veterinaria, 71(1), 61-71.
  • Huang, S. Y., Wu, S. M., Xu, M. J., Zhou, D. H., Danba, C., Gong, G., & Zhu, X. Q. (2013). First record of Chlamydia abortus seroprevalence in Tibetan sheep in Tibet, China. Small Ruminant Research, 112(1-3), 243-245.
  • Jonker, A., & Michel, A. L. (2023). Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion. Pathogens, 12(2), 290.
  • Kalender, H., Kiliç, A., Eröksüz, H., Muz, A., Kilinç, Ü., & Taşdemir, B. (2013). Identification of Chlamydophila abortus infection in aborting ewes and goats in Eastern Turkey. Revue de Médecine Vétérinaire, 164(6), 295-301.
  • Kaltenboeck, B., Hehnen, H. R., & Vaglenov, A. (2005). Bovine Chlamydophila spp. infection: do we underestimate the impact on fertility? Veterinary Research Communications, 29, 1-15.
  • Longbottom, D., Livingstone, M., Maley, S., van der Zon, A., Rocchi, M., Wilson, K., ... & Buxton, D. (2013). Intranasal infection with Chlamydia abortus induces dose-dependent latency and abortion in sheep. PloS one, 8(2), 1-11
  • Malal, M. E., (2020). Marmara bölgesinde ruminant abortlarında Chlamydia abortus’un real time PCR ile teşhisi ve multilokus vntr analizi ile genotiplendirilmesi. PhD thesis, University of Adnan Menderes, Aydın, Türkiye.
  • Marzok, M., Hereba, A. M., Kandeel, M., & Selim, A. (2023). Serosurvey and associated risk factors for Chlamydia abortus infection in cattle from Egypt. Comparative Immunology, Microbiology and Infectious Diseases, 96, 1-5.
  • Sargison, N. D., Truyers, I. G. R., Howie, F. E., Thomson, J. R., Cox, A. L., Livingstone, M., & Longbottom, D. (2015). Identification of the 1B vaccine strain of Chlamydia abortus in aborted placentas during the investigation of toxaemic and systemic disease in sheep. New Zealand Veterinary Journal, 63(5), 284-287.
  • Öztürk, D., Türütoğlu, H., & Kaya, M. (2016). Seroprevalence of Chlamydophila abortus infection in sheep in Burdur province. Veterinary Journal of Mehmet Akif Ersoy University, 1(2), 17-20
  • Wilson, K., Livingstone, M., & Longbottom, D. (2009). Comparative evaluation of eight serological assays for diagnosing Chlamydophila abortus infection in sheep. Veterinary Microbiology, 135(1-2), 38-45.

Investigation of Chlamydia abortus from aborted ewes in Hakkâri province and its districts by enzyme-linked immunosorbent assay (ELISA)

Year 2025, Volume: 9 Issue: 2, 75 - 87, 31.08.2025

Abstract

Iaborted during lambing period in different flocks in Hakkari province and it’s district in Türkiye. Blood samples were collected from ewes that had aborted, using the jugular vein in accordance with the technique. The serum obtained from the blood samples collected in biochemical serum tubes was tested for antibodies against Chlamydia abortus using an ELISA test kit (Sunlong sheep Chlamydia abortus ELISA test kit). According to the ELISA test results, 8 samples (4.4%) out of 180 samples collected from Hakkari province and its districts tested positive for C. abortus. The distribution of samples that tested positive for C. abortus according to the ELISA test results was as follows: 4 sheep in the central district of Hakkari, 3 sheep in the district of Yüksekova, and 1 sheep in the district of Şemdinli tested positive for C. abortus. As a result, for the first time, data on the prevalence of C. abortus in sheep farms in Hakkari province center, Yüksekova and Şemdinli districts were obtained. The data obtained were shared with the animal health branch directorates in the agricultural directorates in Hakkari provinces and districts. Especially in the last decade, it will contribute to the development of activities for the development of preventive measures against C. abortus, which is one of the most important factors causing abortion in sheep farms

Supporting Institution

Bu Çalışma Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

TYL-2023-10809

References

  • Abnaroodheleh, F., Emadi, A., & Dadar, M. (2021). Seroprevalence of brucellosis and chlamydiosis in sheep and goats with history of abortion in Iran. Small Ruminant Research, 202:1-4.
  • Akpınar, R. K., Kılıçoğlu, Y., Şahin, B., Çenesiz, S., Kadı, H., & Çitil, M. (2024). Molecular detection of Chlamydia abortus and investigation of oxidative stress parameters in sheep fetuses in the Black Sea Region of Türkiye. Comparative Clinical Pathology, 33(1), 47-54.
  • Aldama, F. D. J., Montes de Oca Jiménez, R., & Varela Guerrero, J. A. (2022). Diagnóstico, prevención y control de enfermedades causadas por Chlamydia en pequeños rumiantes. Revisión. Revista Mexicana de Ciencias Pecuarias, 13(3), 725-742.
  • Bı̇ngöl, E., & Aygün, T. (2014). Hakkari’de Yetiştirilen Karakaş Koyunlarında Büyüme ve Gelişme Özellikleri. Journal of the Institute of Science and Technology, 4(3), 65-74.
  • Çaya, H., Aslantaş, Ö., İyisan, A. S., Mirioğlu, M., & Tunca, Ş. T. (2006). Chlamydophıla Abortus'a (Chlamydıa Psıttacı Serotype 1) Karşı Oluşan Antikorların Mikrokomplement Fikzasyon (mCFT) Ve Enzyme-Lınked Immunosorbent Assay (ELISA) İle Araştırılması. Etlik Veteriner Mikrobiyoloji Dergisi, 17(1-2), 7-12.
  • Demir, Y., & Tuncer, S. S. (2022). Hakkâri ilindeki küçükbaş hayvancılık işletmelerinin mevcut durumu ve genel sorunları. Van Veterinary Journal, 33(3), 97-105.
  • Esmaeili, H., Bolourchi, M., & Mokhber-Dezfouli, M. R. (2015). Seroprevalence of Chlamydia abortus infection in sheep and goats in Iran. Iranian Journal of Veterinary Medicine, 9(2), 73-77.
  • Esmaeili, H., Shakeri, A. P., Rad, Z. N., Arani, E. B., Villanueva-Saz, S., Ruiz, H., & Lacasta, D. (2022). Causes of abortion in Iranian sheep flocks and associated risk factors. Veterinary Research Communications, 46(4), 1227-1238.
  • Esmaeili, H., Bolourchi, M., Mokhber-Dezfouli, M. R., & Teimourpour, A. (2021). Detection of Chlamydia abortus and risk factors for infection in small ruminants in Iran. Small Ruminant Research, 197, 1-7.
  • Fayez, M., Elmoslemany, A., Alorabi, M., Alkafafy, M., Qasim, I., Al-Marri, T., & Elsohaby, I. (2021). Seroprevalence and risk factors associated with Chlamydia abortus infection in sheep and goats in Eastern Saudi Arabia. Pathogens, 10(4), 489.
  • GAP, (2023, August 18). Uluslararası tarımsal araştırma ve eğitim merkezi. Retrieved from: https://arastirma.tarimorman.gov.tr/gaputaem
  • Giannitti, F., Anderson, M., Miller, M., Rowe, J., Sverlow, K., Vasquez, M., & Cantón, G. (2016). Chlamydia pecorum: fetal and placental lesions in sporadic caprine abortion. Journal of Veterinary Diagnostic Investigation, 28(2), 184-189.
  • Gülaydın, Ö., Öztürk, C., Ekin, İ. H., İlhan, Z., & İlhan, F. (2023). Investigation of selected bacterial agents causing sheep abortion in the Van Province by RT-PCR and histopathological methods. Acta Veterinaria Brno, 92(1), 69-77.
  • Hakkâri il Tarım ve Orman Müdürlüğü, (2024, may 15) Hayvansal üretim. Retrieved from: https://hakkari.tarimorman.gov.tr/Menu/12/ Hakkâri
  • Hireche, S., Agabou, A., & Bounab, B. E. K. (2022). Chlamydia abortus in dairy cattle: Serostatus and risk factors in Algerian northeastern high plateaus. Veterinaria, 71(1), 61-71.
  • Huang, S. Y., Wu, S. M., Xu, M. J., Zhou, D. H., Danba, C., Gong, G., & Zhu, X. Q. (2013). First record of Chlamydia abortus seroprevalence in Tibetan sheep in Tibet, China. Small Ruminant Research, 112(1-3), 243-245.
  • Jonker, A., & Michel, A. L. (2023). Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion. Pathogens, 12(2), 290.
  • Kalender, H., Kiliç, A., Eröksüz, H., Muz, A., Kilinç, Ü., & Taşdemir, B. (2013). Identification of Chlamydophila abortus infection in aborting ewes and goats in Eastern Turkey. Revue de Médecine Vétérinaire, 164(6), 295-301.
  • Kaltenboeck, B., Hehnen, H. R., & Vaglenov, A. (2005). Bovine Chlamydophila spp. infection: do we underestimate the impact on fertility? Veterinary Research Communications, 29, 1-15.
  • Longbottom, D., Livingstone, M., Maley, S., van der Zon, A., Rocchi, M., Wilson, K., ... & Buxton, D. (2013). Intranasal infection with Chlamydia abortus induces dose-dependent latency and abortion in sheep. PloS one, 8(2), 1-11
  • Malal, M. E., (2020). Marmara bölgesinde ruminant abortlarında Chlamydia abortus’un real time PCR ile teşhisi ve multilokus vntr analizi ile genotiplendirilmesi. PhD thesis, University of Adnan Menderes, Aydın, Türkiye.
  • Marzok, M., Hereba, A. M., Kandeel, M., & Selim, A. (2023). Serosurvey and associated risk factors for Chlamydia abortus infection in cattle from Egypt. Comparative Immunology, Microbiology and Infectious Diseases, 96, 1-5.
  • Sargison, N. D., Truyers, I. G. R., Howie, F. E., Thomson, J. R., Cox, A. L., Livingstone, M., & Longbottom, D. (2015). Identification of the 1B vaccine strain of Chlamydia abortus in aborted placentas during the investigation of toxaemic and systemic disease in sheep. New Zealand Veterinary Journal, 63(5), 284-287.
  • Öztürk, D., Türütoğlu, H., & Kaya, M. (2016). Seroprevalence of Chlamydophila abortus infection in sheep in Burdur province. Veterinary Journal of Mehmet Akif Ersoy University, 1(2), 17-20
  • Wilson, K., Livingstone, M., & Longbottom, D. (2009). Comparative evaluation of eight serological assays for diagnosing Chlamydophila abortus infection in sheep. Veterinary Microbiology, 135(1-2), 38-45.
There are 25 citations in total.

Details

Primary Language English
Subjects Food Biotechnology
Journal Section Research Articles
Authors

Yunus Özer 0009-0000-5883-6765

Süleyman Kozat 0000-0001-5089-2623

Vural Denizhan 0000-0002-0531-9550

Project Number TYL-2023-10809
Publication Date August 31, 2025
Submission Date January 28, 2025
Acceptance Date August 21, 2025
Published in Issue Year 2025 Volume: 9 Issue: 2

Cite

APA Özer, Y., Kozat, S., & Denizhan, V. (2025). Investigation of Chlamydia abortus from aborted ewes in Hakkâri province and its districts by enzyme-linked immunosorbent assay (ELISA). Journal of Istanbul Veterinary Sciences, 9(2), 75-87.

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