Preliminary Wound Healing Activity of Polyherbal Formulation Containing Cinnamon zeylanicum, Centella asiatica and Moringa oleifera

Year 2023, , 204 - 211, 01.09.2023

Gautami Ubhrani , Het Talati , Punitkumar Bhatt , Kishorkumar Sorathia , Bhanubhai Suhagia

https://doi.org/10.52794/hujpharm.1133992

Abstract

In the present work, a polyherbal gel formulation for wound healing activity was developed using 70% methanolic extract of Centella asiatica leaves, methanolic extract of Cinnamomum zeylanicum Nees stem bark as well as oil of Moringa olifera seed. The developed formulation was evaluated for organoleptic parameters like colour, odour, texture and physicochemical parameters like a loss on drying (LOD), pH, viscosity, spreadability, skin irritation study, etc. In-vitro antibacterial activity of both the extracts and oil of M. oleifera was carried out against two pathogenic bacteria Escherichia coli and Staphylococcus aureus by disc diffusion assay using tetracycline as a standard. Apart from that, the wound healing activity of a polyherbal formulation was evaluated by an incision model on wistar rats. The results showed that the polyherbal formulation exhibited dark brown colour with a smooth texture and cinnamon-like odour. The gel showed 9% LOD, pH was found to be 7.5±0.3, viscosity was 4609.5± 13.44 cps at 10 rpm with spindle number 63, and spreadability was found to be 19.44±0.26 (g.cm/s), and no irritation was found on the healthy volunteer’s skin. In the wound healing activity, polyherbal did not affect the normal feed and water uptake. The wound did not show any pus formation throughout the treatment period. In the Planimetry assessment, wounds treated with polyherbal formulation showed a good score at the end of the treatment. Results showed that polyherbal formulation containing 70% methanolic extract of C. asiatica leaves, methanolic extract of C. zeylanicum stem bark as well as oil of M. olifera seed have good wound healing activity.

Keywords

In-vitro antibacterial activity, Polyherbal gel, wound healing, herbal, medicinal plants

Supporting Institution

Dharmsinh Desai University, Nadiad (Gujarat) India

References

• 1. Bhatt, P.R., Pandya, K.B., Patel, U.D., Patel, H.B., Modi, C.M., Javia, B.B. : In vitro antibacterial activity of extracts and alkaloid fraction of Prosopis juliflora (Sw.) DC. leaves, Journal of Phytonanotechnology and Pharmaceutical Sciences, 1(4) (2021) https://doi.org/10.54085/jpps.2021.1.4.2
• 2. Pandya, K.B., Patel, H.B., Bhatt, P.R., Patel, U.D., Modi, C.M. : In vitro antibacterial activity of sixteen medicinal plants collected from nearby region of Junagadh, Gujarat (India), Pharma Innovation, 8(4), 662-667 (2019)
• 3. Patel, H.B., Patel, U.D., Modi, C.M., Javia, B.B., Bhatt, P.R., Pandya, K.B. : In-vitro antimicrobial effect of Adhatoda vasica, Annona squamosa, Aloe vera, Butea monosperma and Prosopis juliflora, World Journal of Pharmaceutical Research, 4(12), 1168-1177 (2015)
• 4. Sharma, A., Khanna, S., Kaur, G., Singh, I. : Medicinal plants and their components for wound healing applications, Future Journal of Pharmaceutical Sciences, 7(1), 53 (2021) https://doi.org/10.1186/s43094-021-00202-w
• 5. Ali, A., Garg, P., Goyal, R., Kaur, G., Li, X., Negi, P., Valis, M., Kuca, K., Kulshrestha, S. : A Novel Herbal Hydrogel Formulation of Moringa oleifera for Wound Healing. Plants, 10(1), 25 (2020). https://doi.org/10.3390/plants10010025
• 6. Ranasinghe, P., Pigera, S., Premakumara, G. S., Galappaththy, P., Constantine, G. R., Katulanda, P. : Medicinal properties of ‘true’ cinnamon (Cinnamomum zeylanicum): A systematic review, BMC Complementary and Alternative Medicine, 13(1), 275 (2013). https://doi.org/10.1186/1472-6882-13-275
• 7. Sarker, S. D., Latif, Z., & Gray, A. I., “Natural Products Isolation”, Humana Press, (2005). https://doi.org/10.1385/1592599559
• 8. Pawar, D., Shamkuwar, P.B. : Formulation and evaluation of herbal gel containing Lantana camara leaves extract, Asian Journal of Pharmaceutical and Clinical Research, 6(3), 122-124 (2013).
• 9. Aiyalu, R., Govindarjan, A., Ramasamy, A. : Formulation and evaluation of topical herbal gel for the treatment of arthritis in animal model, Brazilian Journal of Pharmaceutical Sciences, 52(3), 493–507 (2016).. https://doi.org/10.1590/s1984-82502016000300015
• 10. Azis, H.A., Taher, M., Ahmed, A.S., Sulaiman, W.M.A. W., Susanti, D., Chowdhury, S.R., Zakaria, Z.A. : In vitro and In vivo wound healing studies of methanolic fraction of Centella asiatica extract, South African Journal of Botany, 108, 163–174 (2017). https://doi.org/10.1016/j.sajb.2016.10.022
• 11. Dev, S.K., Choudhury, P.K., Srivastava, R., Sharma, M. : Antimicrobial, anti-inflammatory and wound healing activity of polyherbal formulation, Biomedicine & Pharmacotherapy, 111, 555–567 (2019).. https://doi.org/10.1016/j.biopha.2018.12.075
• 12. Rajput, R.T., Gohil, K.J. : Development and evaluation of herbomineral ointment from Bauhinia variegata L. for wound healing effects, Indian Journal of Natural Products and Resources, 11(2), 96-100 (2020).
• 13. Montazer, M., Navaei, T., Mahmoudi rad, M. : Preparation of a naturally driven cotton wound dressing via honey, Tragacanth and Sumac, Indian Journal of Fibre & Textile Research, 45(3), 260-266 (2020).
• 14. Gutierrez, R.M., Baculi, R., Pastor, N., Puma-at, T., Balangcod, T. : Antibacterial potential of some medicinal plants of the Cordillera Region, Philippines, Indian Journal of Traditional Knowledge, 12(4), 630-637 (2013).
• 15. Senthilkumar, M., Goorumurthi, P., Janandharnan, K. : Antibacterial potential of some plants used by tribals in Muruthamalai Hills, Tamil Nadu, Natural Product Radiance, 4(1), 27-34 (2005).
• 16. Goyal, B.R., Agrawal, B.B., Goyal, R.K., Mehta, A.A., Phyto-Pharmacology of Moringa oleifera Lam.- An overview, Natural Product Radiance, 6(4), 347-353 (2007).
• 17. Bisht, D., Mehrotra, R., Singh, P.A., Atri, S.C., Kumar, A. : Effect of helium-neon laser on wound healing, Indian Journal of Experiemntal Biology, 37, 187-189 (1999).
• 18. Barua, C.C., Talukdar, A., Begum, S.A., Sarma, D.K., Pathak, D.C., Barua, A.G., Bora, R.S. : Wound healing activity of methanolic extract of Alternantherana brasiliana Kuntz using in vivo and in vitro model, Indian Journal of Experimental Biology, 47, 1001-1005 (2009).
• 19. Kumar, A., Prakash, D., Kumar, P. : Wound healing activity Solanum xanthocarpum Schrad. & Wendl. fruits, Indian Journal of Natural Products and Resource, 1(4), 470-475 (2010).
• 20. Wahyuni, I. S., Sufiawati, I., Nittayananta, W., Levita, J. : Anti-Inflammatory Activity and Wound Healing Effect of Kaempferia galanga L. Rhizome on the Chemical-Induced Oral Mucosal Ulcer in Wistar Rats, Journal of inflammation research, 15, 2281–2294 (2022). https://doi.org/10.2147/JIR.S359042