Comparative acute toxicity study of Syringodium isoetifolium on aquatic and rodent experimental animals

Year 2023, , 221 - 231, 01.09.2023

Dhanaraju Kavitha , Ramakrishnan Padmini , Veramaneni Alekhya , Chandravadivelu Gopi , Magharla Dasaratha Dhanaraju

https://doi.org/10.52794/hujpharm.1140865

Abstract

This study was designed to explore toxic potential associated with hydroalcoholic extract of Syringodium isoetifolium seagrass (HAESI) on brine shrimp, zebrafish and Wistar albino rats according to the OECD guidelines. The different concentrations of extract were applied to brine shrimp (0.01-1 mg/ml), zebrafish (12.5, 25, 50, 100, 200, 400 mg/ml) and Wistar albino rat (500, 1000, 2000, 2500 and 5000 mg/kg). The control group received distilled water and the studies were carried out as per the OECD guidelines [203 and 423]. The experimental subjects were observed individually for the first 24 hours, with special attention given during the first four hours, thereafter for a prescribed duration. The results of brine shrimp exhibited increased mortality with the increasing concentrations of the extract. Maximum mortality occurred at 1000 Microgram per milliliter (μg/ml) and least mortality happened at 1 μg/ml concentration. The toxic effect on brine shrimp due to the poor elimination of cytotoxic substances from the body at high concentrations and elimination freely occur at low concentrations. Whereas no mortality and behavioural change were observed in the zebrafish and Wistar albino rats irrespective of the concentration.

Keywords

Toxicological study, Syringodium isoetifolium, Hydroalcoholic extract, Aquatic animals, Rodent animals

Supporting Institution

GIET School of Pharmacy, Rajamahendravaram, Andhra Pradesh, India Pin-533296; 1Department of Biochemistry, Vels institute of science, technology and advanced studies, Pallavaram, Chennai -600117, Tamilnadu, India.

Thanks

All authors wish to express their gratitude to the GIET School of Pharmacy, Rajahmundry and 1Department of Biochemistry, Vels institute of science, technology and advanced studies, Pallavaram, Chennai -600117.

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