Daidzein alleviates isoproterenol-induced cardiac injury in rats via NF-kB pathway inhibition

Year 2024, , 124 - 130, 01.06.2024

Vadivelan Ramachandran , Vikash Vaikunth , Tharani Mohanasundaram , Ruchı Tıwarı , Harshini Magesh

https://doi.org/10.52794/hujpharm.1338923

Abstract

The current study set out to explore the anti-inflammatory effects of Daidzein (Dz) on the isoproterenol (Iso)-induced myocardial infarction (MI) in male Wistar rats. Iso(80 mg/kg) was injected subcutaneously into rats to cause MI for two days at intervals of 24 hours. ELISA kits were used to measure the amounts of TNF-α & IL-6 in cardiac tissues. The quantity of NF-kB in the cytosol and nuclei was measured using a western blot approach. H&E staining was used to identify histological cardiac changes. In rats with Iso-induced myocardial infarction, Dz therapy shrunk the size of the infarct and reduced histological anomalies in the myocardium. Dz significantly lowered cardiac pro-inflammatory cytokine levels and prevented NF-kB nuclear translocation in MI-damaged rats, substantially reversing Iso-induced damage. This research shows that daidzein treatment in vivo reduces Iso-induced myocardial damage by preventing NF-kB activation, which may therefore reduce the release of inflammatory cytokines.

Keywords

daidzein, izoproterenol, miyokardiyal yaralanma, iltihaplanma

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