Prangos uechtritzii Boiss & Hausskn'dan İzole Edilmiş Bileşiklerin Sitotoksik Aktiviteleri

Abstract

Prangos uechtritzii Boiss&Hausskn Türkiye'nin endemik bir bitkisidir ve bitki kökleri kumarinler açısından zengindir. Bu çalışma, P. uechtritzii'nin n-hekzan (PH), kloroform (PC) ve metanol (PM) ekstreleri ile birlikte 16 doğal bileşiğin sitotoksik aktivitelerini değerlendirmeyi amaçlamaktadır. Bu amaçla on dört kumarin türevi; umbelliferon(1), 6-formilumbelliferon(2), suberosin(3), 7-demetilsuberosin(4), (+)-ulopterol(5), tamarin(6), psoralen(7), imperatorin(8), (+ )-oksipösedanin(9), (+)-oksipösedanin hidrat(10), (+)-oksipösedanin metanolat(11), (+)-marmesin(12), (-)-prantşimgin(13) ve (-)- adikardin(14); iki poliasetilen (-)-panaksinol(15), (+)-falkarindiol(16) ve üç ekstre (PH, PC ve PM), iki sağlıklı (HK-2, NIH/3T3) ve dört kanserli (MCF-7, A-549, SH-SY5Y, PC-3) hücre hattına karşı WST-1 yöntemi kullanılarak sitotoksisite açısından test edilmiştir. Pozitif kontrol olarak doksorubisin kullanılmıştır. PH ve PC, 8.16-91.56 µg/mL IC50 değerleriyle tüm hücre hatlarında sitotoksik etki göstermiştir. PH, NIH/3T3'e kıyasla SH-SY5Y hücreleri üzerinde seçici bir etki göstermiştir [Seçicilik İndeksi (SI)= 2.5]. PC, hem NIH/3T3 hem de HK-2 ile karşılaştırıldığında PC-3 hücreleri (SI=2) üzerinde sitotoksik etkiler sergilemiştir. PM 100 µg/mL'de sitotoksisite göstermemiştir. (-)-Panaksinol, (+)-falkarindiol, 6-formilumbelliferon, 7-demetilsuberosin ve suberosin, IC50= 8.65-87.91 µM değerlerinde etkiler sergilerken diğerleri 100 µM'de etki göstermemiştir. Sonuçlara göre P. uechtritzii, kanser tedavisine yönelik yeni ilaçların geliştirilmesinde umut verici bir doğal kaynak olabilir.

Keywords

• Prangos uechtritzii
• kumarin
• sitotoksisite

Cytotoxic Activities of Isolated Compounds from Prangos uechtritzii Boiss & Hausskn

Abstract

Prangos uechtritzii Boiss&Hausskn is an endemic plant of Türkiye, and the plant roots are rich in coumarins. This study aims to evaluate the cytotoxic activities of the 16 natural compounds along with the n-hexane (PH), chloroform (PC), and methanol (PM) extracts of P. uechtritzii. For this purpose, fourteen coumarin derivatives; umbelliferone(1), 6-formylumbelliferone(2), suberosin(3), 7-demethylsuberosin(4), (+)-ulopterol(5), tamarin(6), psoralen(7), imperatorin( 8), (+)-oxypeucedanin(9), (+)-oxypeucedanin hydrate(10), (+)-oxypeucedanin methanolate(11), (+)-marmesin(12), (-)-prantschimgin(13), and (-)-adicardin( 14); two polyacetylenes (-)-panaxynol(15), (+)-falcarindiol(16) and three extracts (PH, PC, and PM) were tested for their cytotoxicity against two healthy (HK-2, NIH/3T3), and four cancer (MCF-7, A-549, SH-SY5Y, PC-3) cells by WST-1 method. Doxorubicin was used as a positive control. PH and PC showed cytotoxic effects on all the cell lines with IC50 values of 8.16-91.56 μg/mL. PH displayed a selective effect on SH-SY5Y cells [Selectivity Index (SI)= 2.5] compared to NIH/3T3. PC exhibited cytotoxic effects on PC-3 cells (SI=2) compared to both NIH/3T3, and HK-2. PM didn’t display cytotoxicity at 100 μg/mL. (-)-Panaxynol, (+)-falcarindiol, 6-formylumbelliferone, 7-demethylsuberosin, and suberosin exhibited effects with IC50 values of 8.65-87.91 μM, while others didn’t work at 100 μM. So, P. uechtritzii could be a promising natural source in the development of new drugs for cancer treatment.

Keywords

• Prangos uechtritzii
• coumarin
• cytotoxicity

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