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1,25 Dihidroksivitamin D’nin Ratlarda Ağrı Eşiği Üzerine Etkisini Belirlemede: Etkin Doz Çalışması

Year 2021, , 228 - 233, 01.12.2021
https://doi.org/10.52794/hujpharm.999857

Abstract

Vitamin D’nin iskelet sisteminde bilinen etkileri dışında diğer sistemler üzerinde de etkisi yapılan çalışmalarla ortaya çıkarılmış olup, son yıllarda klinik ve hayvan çalışmaları sıklıkla yapılmaktadır. D vitaminin çeşitli formları bulunmakla beraber en aktif ve etkili olanı, 1,25(OH)2D olarak bilinen kalstrioldur. Bu çalışmanın amacı ratlarda kalstriolun ağrı eşiği üzerine etkisinin değerlendirmesinde etkin ve güvenilir dozu saptamaktır. Deneyde 32 adet, Wistar Albino cinsi 2,5 aylık (240-260 gr) erkek sıçan kullanıldı. Hayvanlar her grupta 8 hayvan olacak şekilde rastgele 4 gruba ayrıldı. Gruplar; kontrol, vitamin D= 1 µg/kg, vitamin D= 10 µg/kg ve vitamin D= 100 µg/kg olarak belirlendi. Ağrı eşiğinin değerlendirilmesinde tail flick ve hot plate testleri kullanıldı. Ölçümler ilaç uygulamalarından önce 0. dakika ve ilaç sonrasını takiben 30, 60 ve 90. dakikalarda alındı ve süreler saniye cinsinden kaydedildi. Vitamin D= 10 µg/kg uygulanan grup diğer gruplara göre hem tail flick hem de hot plate testinde ağrıya dayanma süresini anlamlı olarak uzattı (p˂0.05). Çalışmamız sonucunda elde edilen etkin doz sayesinde, kalstriol kullanılarak yapılacak deneysel ağrı modellerinde kolaylık sağlanacak ve çalışmacılar için ekstra zaman kaybı ve hayvan kaybı yaşanmadan ileri çalışmalara katkı sağlanmış olacaktır.

Supporting Institution

Erciyes Üniveristesi BAP Birimi

Project Number

TDK-2019-8949

Thanks

Bu çalışma için Erciyes Üniversitesi Proje Koordinasyon Uygulama ve Araştırma Merkezi tarafından TDK-2019- 8949 numaralı BAP projesi desteği sağlandı.

References

  • 1.Tellioğlu A, Başaran S: Güncel Bilgiler Işığında Vitamin D. Arşiv Kaynak Tarama Dergisi 2013, 22(2):259-271.
  • 2. Jones G, Prosser DE: Kaufmann M. Cytochrome P450-mediated metabolism of vitamin D. J Lipid Res 2014, 55:13–31.
  • 3. Özçelik D, Koçer H, Kasım İ, Şencan İ, Kahveci R, Özkara A: D vitamini. Turkish Medical Journal 2012, 6:61-67.
  • 4. Plotnikoff GA, Quigley JM: Prevalence of severe hypovitaminosis D in patients with persistent, nonspecific musculoskeletal pain. In Mayo clinic proceedings 2003, 78:1463-1470.
  • 5. Lotfi A, Abdel-Nasser AM, Hamdy A, Omran AA, El-Rehany MA: Hypovitaminosis D in female patients with chronic low back pain. Clinical rheumatology 2007, 26:1895-1901.
  • 6. Saps M, Blank C, Khan S, Seshadri R, Marshall BM, Bass LM, et al: Seasonal variation in the presentation of abdominal pain. Journal of pediatric gastroenterology and nutrition 2008, 46:279-284.
  • 7. Mitsikostas DD, Tsaklakidou D, Athanasiadis N, Thomas A: The prevalence of headache in Greece: correlations to latitude and climatological factors. Headache 1996, 36:168–173.
  • 8. Zeng QY, Chen R, Xiao Z: Low prevalence of knee and back pain in southeast China; the Shantou COPCORD study. The Journal of Rheumatology 2004, 31:249–253.
  • 9. Glerup H, Mikkelsen K, Poulsen L: Commonly recommended daily intake of vitamin D is not sufficient if sunlight exposure is limited. Journal of Internal Medicine 2000, 247:260-268.
  • 10. Arvold DS, Odean MJ, Dornfeld MP: Corelation of symptoms with vitamin D deficiency and symptom response to cholecalciferol treatment: a randomized controlled trial. Endocrine Practice 2009,15:203–212.
  • 11. Oh RC, Johnson JD: Chest pain and costochondritis associated with vitamin D deficiency: a report of two cases. Case Reports in Medicine 2012.
  • 12. Waikakul S: Serum 25-hydroxy-calciferol level and failed back surgery syndrome. Journal of Orthopaedic Surgery 2012, 20:18–22.
  • 13. D’Amour FE, Smith DL: A method for determining loss of pain sensation. J Pharmacol Exp Ther 1941, 72:74-79.
  • 14. Tjolsen A, Lund A, Berge O-G, and Hole K: An improved method for tail-flick testing with adjustment for tail-skin temperature. J Neurosci Methods 1989, 26:259-265.
  • 15. Eddy NB, Leimback D: Synthetic analgesics. II. Dithinylbutenyl and Dithinylbuteny58 lamines. J Pharmacol Exp Ther 1953, 107: 385-393.
  • 16. Vieth R, Chan P, MacFarlane G: Efficacy and safety of vitamin D3 intake exceeding the lowest observed adverse effect level1-3. Am J Clin Nutr 2001, 73:288-294.
  • 17. Vieth R, Kimball S, Hu A, Walfish P: Randomized comparison of the effect of the vitamin D3 adequate intake versus 100 mcg (4000 IU) per day on biochemical responses and the wellbeing of patient. Nutrition Journal 2004, 3:8.
  • 18. Zhu CG, Liu YX, Wang H, Wang BP, Qu HQ, Wang BL, Zhu M: Active form of vitamin D ameliorates non-alcoholic fatty liver disease by alleviating oxidative stress in a high-fat diet rat model. Endocrine journal 2017, 64(7): 663-673.
  • 19. Han H, Cui M, You X, Chen M, Piao X, Jin G: A role of 1, 25 (OH) 2D3 supplementation in rats with nonalcoholic steatohepatitis induced by choline-deficient diet. Nutrition, Metabolism and Cardiovascular Diseases 2015, 25(6):556-561.
  • 20. Ma Ming, Long Qi, Che Fei, Zhang Ting, Wang Wenqiao: Active vitamin D impedes the progression of non-alcoholic fatty liver disease by inhibiting cell senescence in a rat model. Clinics and Research in Hepatology and Gastroenterology 2019, 44(4):513-523.
  • 21. Taghizadeh M, Djazayery A, Salami M, Eshraghian MR, Zavareh SAT: Vitamin-D-free regimen intensifies the spatial learning deficit in Alzheimer's disease. International Journal of Neuroscience 2011, 121(1):16-24.
  • 22. Babaei P, Shirkouhi SG, Hosseini R, Tehrani BS. Vitamin D is associated with metabotropic but not neurotrophic effects of exercise in ovariectomized rats. Diabetology & metabolic syndrome 2017, 9(1):1-9.
  • 23. Shi W, Guo L, Liu G, Peng T, Li H, Xie T, Yang X: Protective effect of calcitriol on podocytes in spontaneously hypertensive rat. Journal of the Chinese Medical Association 2018, 281(8):691-698.
  • 24. Mehri N, Haddadi R, Ganji M, Shahidi S, Asl SS, Azandariani MT, Ranjbar A: Effects of vitamin D in an animal model of Alzheimer’s disease behavioral assessment with biochemical investigation of Hippocampus and serum. Metabolic brain disease 2020, 35(2):263-274.
  • 25. Wang D, Li Y, Wang N, Luo G, Wang J, Luo C, Hao L: 1α, 25-Dihydroxyvitamin D3 prevents renal oxidative damage via the PARP1/SIRT1/NOX4 pathway in Zucker diabetic fatty rats. American Journal of Physiology-Endocrinology and Metabolism 2020, 318(3):E343-E356.
  • 26. Zhou Yan, Zhou Xin, Wang Xiaoqiu, 1, 25-Dihydroxyvitamin D 3 prevented allergic asthma in a rat model by suppressing the expression of inducible nitric oxide synthase. In: Allergy & Asthma Proceedings; 2008.

Determining the Effect of 1,25 Dihydroxyvitamin D on Pain Threshold in Rats: An Effective Dose Study

Year 2021, , 228 - 233, 01.12.2021
https://doi.org/10.52794/hujpharm.999857

Abstract

In addition to the known effects of vitamin D on the skeletal system, its effects on other systems have been revealed by studies, and clinical and animal studies have been carried out frequently in recent years. Although there are various forms of vitamin D, the most active and effective is calstriol, known as 1,25(OH)2D. The aim of this study is to determine the effective and safe dose for the evaluation of the effect of calstriol on pain threshold in rats. In the experiment, 32 Wistar Albino male rats, 2.5 months old (240-260 gr) were used. The animals were randomly divided into 4 groups with 8 animals in each group. Groups; control, vitamin D= 1 µg/kg, vitamin D= 10 µg/kg and vitamin D= 100 µg/kg. Tail flick and hot plate tests were used to evaluate the pain threshold. Measurements were taken at the 0th minute before the drug administration and at the 30th, 60th and 90th minutes after the drug administration, and the times were recorded in seconds. Vitamin D= 10 µg/kg administered group significantly prolonged the pain tolerance time in both tail flick and hot plate test compared to the other groups (p˂0.05). Thanks to the effective dose obtained as a result of our study, experimental pain models using calstriol will be facilitated and further studies will be contributed without wasting extra time and animal loss for the researchers.

Project Number

TDK-2019-8949

References

  • 1.Tellioğlu A, Başaran S: Güncel Bilgiler Işığında Vitamin D. Arşiv Kaynak Tarama Dergisi 2013, 22(2):259-271.
  • 2. Jones G, Prosser DE: Kaufmann M. Cytochrome P450-mediated metabolism of vitamin D. J Lipid Res 2014, 55:13–31.
  • 3. Özçelik D, Koçer H, Kasım İ, Şencan İ, Kahveci R, Özkara A: D vitamini. Turkish Medical Journal 2012, 6:61-67.
  • 4. Plotnikoff GA, Quigley JM: Prevalence of severe hypovitaminosis D in patients with persistent, nonspecific musculoskeletal pain. In Mayo clinic proceedings 2003, 78:1463-1470.
  • 5. Lotfi A, Abdel-Nasser AM, Hamdy A, Omran AA, El-Rehany MA: Hypovitaminosis D in female patients with chronic low back pain. Clinical rheumatology 2007, 26:1895-1901.
  • 6. Saps M, Blank C, Khan S, Seshadri R, Marshall BM, Bass LM, et al: Seasonal variation in the presentation of abdominal pain. Journal of pediatric gastroenterology and nutrition 2008, 46:279-284.
  • 7. Mitsikostas DD, Tsaklakidou D, Athanasiadis N, Thomas A: The prevalence of headache in Greece: correlations to latitude and climatological factors. Headache 1996, 36:168–173.
  • 8. Zeng QY, Chen R, Xiao Z: Low prevalence of knee and back pain in southeast China; the Shantou COPCORD study. The Journal of Rheumatology 2004, 31:249–253.
  • 9. Glerup H, Mikkelsen K, Poulsen L: Commonly recommended daily intake of vitamin D is not sufficient if sunlight exposure is limited. Journal of Internal Medicine 2000, 247:260-268.
  • 10. Arvold DS, Odean MJ, Dornfeld MP: Corelation of symptoms with vitamin D deficiency and symptom response to cholecalciferol treatment: a randomized controlled trial. Endocrine Practice 2009,15:203–212.
  • 11. Oh RC, Johnson JD: Chest pain and costochondritis associated with vitamin D deficiency: a report of two cases. Case Reports in Medicine 2012.
  • 12. Waikakul S: Serum 25-hydroxy-calciferol level and failed back surgery syndrome. Journal of Orthopaedic Surgery 2012, 20:18–22.
  • 13. D’Amour FE, Smith DL: A method for determining loss of pain sensation. J Pharmacol Exp Ther 1941, 72:74-79.
  • 14. Tjolsen A, Lund A, Berge O-G, and Hole K: An improved method for tail-flick testing with adjustment for tail-skin temperature. J Neurosci Methods 1989, 26:259-265.
  • 15. Eddy NB, Leimback D: Synthetic analgesics. II. Dithinylbutenyl and Dithinylbuteny58 lamines. J Pharmacol Exp Ther 1953, 107: 385-393.
  • 16. Vieth R, Chan P, MacFarlane G: Efficacy and safety of vitamin D3 intake exceeding the lowest observed adverse effect level1-3. Am J Clin Nutr 2001, 73:288-294.
  • 17. Vieth R, Kimball S, Hu A, Walfish P: Randomized comparison of the effect of the vitamin D3 adequate intake versus 100 mcg (4000 IU) per day on biochemical responses and the wellbeing of patient. Nutrition Journal 2004, 3:8.
  • 18. Zhu CG, Liu YX, Wang H, Wang BP, Qu HQ, Wang BL, Zhu M: Active form of vitamin D ameliorates non-alcoholic fatty liver disease by alleviating oxidative stress in a high-fat diet rat model. Endocrine journal 2017, 64(7): 663-673.
  • 19. Han H, Cui M, You X, Chen M, Piao X, Jin G: A role of 1, 25 (OH) 2D3 supplementation in rats with nonalcoholic steatohepatitis induced by choline-deficient diet. Nutrition, Metabolism and Cardiovascular Diseases 2015, 25(6):556-561.
  • 20. Ma Ming, Long Qi, Che Fei, Zhang Ting, Wang Wenqiao: Active vitamin D impedes the progression of non-alcoholic fatty liver disease by inhibiting cell senescence in a rat model. Clinics and Research in Hepatology and Gastroenterology 2019, 44(4):513-523.
  • 21. Taghizadeh M, Djazayery A, Salami M, Eshraghian MR, Zavareh SAT: Vitamin-D-free regimen intensifies the spatial learning deficit in Alzheimer's disease. International Journal of Neuroscience 2011, 121(1):16-24.
  • 22. Babaei P, Shirkouhi SG, Hosseini R, Tehrani BS. Vitamin D is associated with metabotropic but not neurotrophic effects of exercise in ovariectomized rats. Diabetology & metabolic syndrome 2017, 9(1):1-9.
  • 23. Shi W, Guo L, Liu G, Peng T, Li H, Xie T, Yang X: Protective effect of calcitriol on podocytes in spontaneously hypertensive rat. Journal of the Chinese Medical Association 2018, 281(8):691-698.
  • 24. Mehri N, Haddadi R, Ganji M, Shahidi S, Asl SS, Azandariani MT, Ranjbar A: Effects of vitamin D in an animal model of Alzheimer’s disease behavioral assessment with biochemical investigation of Hippocampus and serum. Metabolic brain disease 2020, 35(2):263-274.
  • 25. Wang D, Li Y, Wang N, Luo G, Wang J, Luo C, Hao L: 1α, 25-Dihydroxyvitamin D3 prevents renal oxidative damage via the PARP1/SIRT1/NOX4 pathway in Zucker diabetic fatty rats. American Journal of Physiology-Endocrinology and Metabolism 2020, 318(3):E343-E356.
  • 26. Zhou Yan, Zhou Xin, Wang Xiaoqiu, 1, 25-Dihydroxyvitamin D 3 prevented allergic asthma in a rat model by suppressing the expression of inducible nitric oxide synthase. In: Allergy & Asthma Proceedings; 2008.
There are 26 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences
Journal Section Research Articles
Authors

Seda Gündüz Başçıl

Asuman Gölgeli 0000-0002-9004-8563

Project Number TDK-2019-8949
Publication Date December 1, 2021
Acceptance Date November 12, 2021
Published in Issue Year 2021

Cite

Vancouver Gündüz Başçıl S, Gölgeli A. Determining the Effect of 1,25 Dihydroxyvitamin D on Pain Threshold in Rats: An Effective Dose Study. HUJPHARM. 2021;41(4):228-33.