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Porphyridium cruentum’un Antimikrobiyal ve Antioksidan Aktivitesi

Year 2017, Volume , Issue 1, 1 - 7, 01.01.2017

Abstract

Bu çalışmada, Porphyridium cruentum’un kültürünün yapılması, potansiyel antimikrobiyal ve antioksidan aktivitesinin analiz edilmesi amaçlandı. P. cruentum kültürlerinden elde edilen biyokütle, etanol, n-hekzan, metanol ekstreleri ve uçucu komponentlerin eldesi için işlemlerden geçirildi. Ekstrelerin antimikrobiyal, antioksidan aktiviteleri ve total fenolik içeriği in vitro test metodları ile araştırıldı. Ayrıca P. cruentum’un uçucu bileşenleri GC/GC-MS metodu ile aydınlatıldı. Diğer çözgen ekstreleri ile kıyaslandığında, sadece n-hekzan ekstresinde potansiyel antimikrobiyal aktivite gözlendi. Ayrıca, bu ekstre en iyi etkiyi Staphylococcus aureus ATCC 6538P’ye karşı gösterdi. Uçucu kısımları sadece Pseudomonas aeruginosa ATCC 27853’e karşı etkiliydi. Total fenolik bileş ikler 62,45±2,0 mg Gallik Asit Eşdeğer/g Kuru Ağırlık mgGAE/gKA değeri ile en yüksek oranda n-hekzan ekstresinde bulundu. İstatistiksel olarak, etanol ekstresinin, radikal süpürme kapasitesi ve total fenolik içeriği arasında önemli bir korelasyon olduğu tespit edildi. Ekstrelerin antioksidan aktivite düzeyi test metoduna bağlı olarak farklılıklar gösterdi. Sonuç olarak, P. cruentum’un, potansiyel değerli antioksidan, antimikrobiyal içeriği ile doğal bileşiklerin önemli bir orijini ve yüksek bitkiler gibi diğer doğal kaynaklara güçlü bir alternatif olarak kabul edilebileceği düşünüldü.

References

  • Apt KE, Behrens PW: Commerc al Developments n M croalgal B otechnology. Journal of Phycology 1999, 35: 215-226.
  • Ördög V, Stirk WA, Lenobel R, Bancirova M, Strnad M, Staden J, Szigeti J, Nemeth L: Screening microalgae for some potentially useful agricultural and pharmaceutical secondary metabolites. Journal of Applied Phycology 2004, 16: 309-314.
  • Spolaore P, Joannis-Cassan C, Duran E, Isambert A: Commercial applications of microalgae. Journal of Bioscience and Bioengineering 2006, 101: 87-96.
  • Vonshak A. Porphyridium. In: Borowitzka MA, Borowitzka LJ. (eds), Microalgal Biotechnology. Cambridge University Press; Cambridge, UK. 1988: pp 122-134 .
  • Ginzberg A, Cohen M, Sod-Moriah UA, Shany S, Rosenshtrauch A, Arad S(M): Chickens fed with biomass of the red microalga Porphyridium sp. have reduced blood cholesterol level and modified fatty acid composition in egg yolk. Journal of Applied Phycology 2000, 12: 325-330.
  • Dufosse’ L, Galaup P, Yaron A, Arad S (M), Blanc PK, Murthy NC, Ravishankar GA: Microorganisms and microalgae as sources of pigments for food use: a scientific oddity or an industrial reality? Trends in Food Science and Technology 2005, 16: 389-406.
  • Huleihel M, Ishanu V, Tal J, Arad S(M): Antiviral Effect of Red Microalgal Polysaccharides on Herpes simplex and Varicella zoster Viruses. Journal of Applied Phycology 2001, 13: 127-134.
  • Bergé JP, Debiton E, Dumay J, Durand P, Barthomeuf CJ: In vitro anti-inflammatory and anti- proliferative activity of sulfolipids from the red alga Porphyridium cruentum. Agricultural Food Chemistry 2002, 50: 6227-6232 .
  • Siriwardhana N, Lee K-W, Jeon Y-J, Kim S-H, Haw J-W: Antioxidant activity of Hizikia fusiformis on reactive oxygen species scavenging and lipid peroxidation inhibition. Food Science and Technology International 2003, 9: 339-346.
  • Velioglu YS, Mazza G, Gao L, Oomah BD: Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry 1998, 46: 4113- 4117.
  • Li H-B, Cheng K-W, Wong C-C, Fan K-W, Chen F, Jiang Y: Evaluat on of ant ox dant capac ty and total phenol c content of d fferent fract ons of selected m croalgae. Food Chemistry 2007, 102: 771-776.
  • Almajano MP, Carbo R, Lopez JA, Gordon MH: Antioxidant and antimicrobial activities of tea infusions. Food Chemistry 2008, 108: 55-63.
  • Sroka Z, Cisowski W: Hydrogen peroxide scavenging, antioxidant and antiradical activity of some phenolics acids. Food and Chemical Toxicology 2003, 41: 753-758.
  • Guillard RRL, Ryther JH: Studies of marine planktonic diatoms. I. Cyclotella nana hustedt and detonula confervacea cleve. Canadian Journal of Microbiology 1962, 8: 229-239.
  • Ozdemir G, Karabay NU, Conk Dalay M, Pazarbasi B: Antibacterial activity of volatile component and various extracts of Spirulina platensis. Phytotherapy Research 2004, 18: 754-757.
  • Adams RP: Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. Allured Publishing Corp. Carol Stream, Illinois, USA, 1995.
  • Bradshaw LJ: Laboratory Microbiology. Saunders College Publishing; Emeritus California State University, Fullerton, New York, USA, 1992.
  • Collins CM, Lyne PM: Microbiological Methods. Butterworths and Co. Ltd., London, UK, 1989.
  • Brand-Williams W, Cuvelier ME, Berset C: Use of a Free Radical Method to Evaluate Antioxidant Activity. LWT-Food Science Technology 1995, 28: 25-30.
  • Ismail A, Hong TS: Antioxidant activity of selected commercial seaweeds. Malaysian Journal of Nutrition 2002, 8: 167-177.
  • Durmaz Y, Monteiro M, Bandarra N, Gökpınar Ş, Işık O: The effect of low temperature on fatty acid composition and tocopherols of the red microalga, Porphyridium cruentum. Journal of Applied Phycology 2007, 19: 223-227.
  • Kamenarska Z, Dimitrova-Konaklieva S, Stefanov K, Najdenski H, Tzvetkova I, Popov S: Comparative Study of The Volatile Compounds From Some Black Sea Brown Algae. Botanica Marina 2002, 45: 502-509.
  • Katırcıoglu H, Beyatlı Y, Aslım B, Yuksekdag Z, Atıcı T: Screening for Antimicrobial Agent Production of Some Microalgae in Freshwater, Internet Journal of Microbiology 2006, 2: 2.
  • Karabay-Yavasoglu NU, Sukatar A, Ozdemir G, Horzum Z: Antimicrobial Activity of Volatile Components and Various Extracts of the Red Alga Jania rubens. Phytotherapy Research 2007, 21: 153-156.
  • Kreitlow S, Mundt S, Lindequist U: Cyanobacteria-A Potencial Source of New Biologically Active Substances. Journal of Biotechnology 1999, 70: 61-63 .
  • Rodriguez-Garcia I, Guil-Guerrero JL: Evaluation of the Antioxidant Activity of Three Microalgal Species for Use as Dietary Supplements and in the Preservation of Foods. Food Chemistry 2008, 108: 1023- 1026.
  • Tannin-Spitz T, Bergman M, Moppes D, Grossman S, Arad S(M): Antioxidant Activity of the Polysaccharide of the Red Microalga Porphyridium sp., Journal of Applied Phycology 2005, 17: 215-222 .
  • Vijayavel K, Anbuselvam C, Balasubramanian MP: Antioxidant Effect of the Marine Algae Chlorella vulgaris Against Naphthalene-induced Oxidative Stress in the Albino Rats, Molecular Cell Biochemistry 2007, 303: 39-44.
  • Wu L-C, Annie HJ-A, Shieh C-H, Lu I-W: Antioxidant and Antiproliferative Activities of Spirulina and Chlorella Water Extracts, Journal of Agricultural Food Chemistry 2005, 53: 4207-4212.
  • Sengul M, Yıldız H, Gungor N, Cetin B, Eser Z, Ercisli S: Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pakistan Journal of Pharmaceutical Sciences 2009, 1: 102-106.

Antimicrobial and Antioxidant Activities of Porphyridium cruentum

Year 2017, Volume , Issue 1, 1 - 7, 01.01.2017

Abstract

In the present study, it was aimed to cultivate and analyze the potential antimicrobial and antioxidant activity of Porphyridium cruentum. The biomass achieved from P. cruentum cultures was processed to obtain ethanol, n-hexane, methanol extracts and volatile components. Antimicrobial, antioxidant activities and total phenolic contents of the extracts were investigated by in vitro test methods. In addition, volatile compounds of P. cruentum were elucidated by GC/GCMS. When compared to the other solvent extracts, a potential antimicrobial activity was observed only in the n-hexane extract. Besides, it showed the best activity against Staphylococcus aureus ATCC 6538P. Volatile parts were active only against Pseudomonas aeruginosa ATCC 27853. The highest amount of total phenolic compounds was found as 62.45±2.0 mg Gallic Acid Equivalent/g Dry Weight mgGAE/gDW in the n-hexane extract. Statistically, a remarkable correlation was established between radical scavenging capacities and the total phenolic contents of the ethanol extracts. The level of antioxidant capacities varied according to the test methods. Consequently, it was thought that P. cruentum could be considered as an important origin of natural compounds and a strong alternative to the other natural sources such as higher plants, because of potentially valuable antioxidant, antimicrobial constutients.

References

  • Apt KE, Behrens PW: Commerc al Developments n M croalgal B otechnology. Journal of Phycology 1999, 35: 215-226.
  • Ördög V, Stirk WA, Lenobel R, Bancirova M, Strnad M, Staden J, Szigeti J, Nemeth L: Screening microalgae for some potentially useful agricultural and pharmaceutical secondary metabolites. Journal of Applied Phycology 2004, 16: 309-314.
  • Spolaore P, Joannis-Cassan C, Duran E, Isambert A: Commercial applications of microalgae. Journal of Bioscience and Bioengineering 2006, 101: 87-96.
  • Vonshak A. Porphyridium. In: Borowitzka MA, Borowitzka LJ. (eds), Microalgal Biotechnology. Cambridge University Press; Cambridge, UK. 1988: pp 122-134 .
  • Ginzberg A, Cohen M, Sod-Moriah UA, Shany S, Rosenshtrauch A, Arad S(M): Chickens fed with biomass of the red microalga Porphyridium sp. have reduced blood cholesterol level and modified fatty acid composition in egg yolk. Journal of Applied Phycology 2000, 12: 325-330.
  • Dufosse’ L, Galaup P, Yaron A, Arad S (M), Blanc PK, Murthy NC, Ravishankar GA: Microorganisms and microalgae as sources of pigments for food use: a scientific oddity or an industrial reality? Trends in Food Science and Technology 2005, 16: 389-406.
  • Huleihel M, Ishanu V, Tal J, Arad S(M): Antiviral Effect of Red Microalgal Polysaccharides on Herpes simplex and Varicella zoster Viruses. Journal of Applied Phycology 2001, 13: 127-134.
  • Bergé JP, Debiton E, Dumay J, Durand P, Barthomeuf CJ: In vitro anti-inflammatory and anti- proliferative activity of sulfolipids from the red alga Porphyridium cruentum. Agricultural Food Chemistry 2002, 50: 6227-6232 .
  • Siriwardhana N, Lee K-W, Jeon Y-J, Kim S-H, Haw J-W: Antioxidant activity of Hizikia fusiformis on reactive oxygen species scavenging and lipid peroxidation inhibition. Food Science and Technology International 2003, 9: 339-346.
  • Velioglu YS, Mazza G, Gao L, Oomah BD: Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry 1998, 46: 4113- 4117.
  • Li H-B, Cheng K-W, Wong C-C, Fan K-W, Chen F, Jiang Y: Evaluat on of ant ox dant capac ty and total phenol c content of d fferent fract ons of selected m croalgae. Food Chemistry 2007, 102: 771-776.
  • Almajano MP, Carbo R, Lopez JA, Gordon MH: Antioxidant and antimicrobial activities of tea infusions. Food Chemistry 2008, 108: 55-63.
  • Sroka Z, Cisowski W: Hydrogen peroxide scavenging, antioxidant and antiradical activity of some phenolics acids. Food and Chemical Toxicology 2003, 41: 753-758.
  • Guillard RRL, Ryther JH: Studies of marine planktonic diatoms. I. Cyclotella nana hustedt and detonula confervacea cleve. Canadian Journal of Microbiology 1962, 8: 229-239.
  • Ozdemir G, Karabay NU, Conk Dalay M, Pazarbasi B: Antibacterial activity of volatile component and various extracts of Spirulina platensis. Phytotherapy Research 2004, 18: 754-757.
  • Adams RP: Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. Allured Publishing Corp. Carol Stream, Illinois, USA, 1995.
  • Bradshaw LJ: Laboratory Microbiology. Saunders College Publishing; Emeritus California State University, Fullerton, New York, USA, 1992.
  • Collins CM, Lyne PM: Microbiological Methods. Butterworths and Co. Ltd., London, UK, 1989.
  • Brand-Williams W, Cuvelier ME, Berset C: Use of a Free Radical Method to Evaluate Antioxidant Activity. LWT-Food Science Technology 1995, 28: 25-30.
  • Ismail A, Hong TS: Antioxidant activity of selected commercial seaweeds. Malaysian Journal of Nutrition 2002, 8: 167-177.
  • Durmaz Y, Monteiro M, Bandarra N, Gökpınar Ş, Işık O: The effect of low temperature on fatty acid composition and tocopherols of the red microalga, Porphyridium cruentum. Journal of Applied Phycology 2007, 19: 223-227.
  • Kamenarska Z, Dimitrova-Konaklieva S, Stefanov K, Najdenski H, Tzvetkova I, Popov S: Comparative Study of The Volatile Compounds From Some Black Sea Brown Algae. Botanica Marina 2002, 45: 502-509.
  • Katırcıoglu H, Beyatlı Y, Aslım B, Yuksekdag Z, Atıcı T: Screening for Antimicrobial Agent Production of Some Microalgae in Freshwater, Internet Journal of Microbiology 2006, 2: 2.
  • Karabay-Yavasoglu NU, Sukatar A, Ozdemir G, Horzum Z: Antimicrobial Activity of Volatile Components and Various Extracts of the Red Alga Jania rubens. Phytotherapy Research 2007, 21: 153-156.
  • Kreitlow S, Mundt S, Lindequist U: Cyanobacteria-A Potencial Source of New Biologically Active Substances. Journal of Biotechnology 1999, 70: 61-63 .
  • Rodriguez-Garcia I, Guil-Guerrero JL: Evaluation of the Antioxidant Activity of Three Microalgal Species for Use as Dietary Supplements and in the Preservation of Foods. Food Chemistry 2008, 108: 1023- 1026.
  • Tannin-Spitz T, Bergman M, Moppes D, Grossman S, Arad S(M): Antioxidant Activity of the Polysaccharide of the Red Microalga Porphyridium sp., Journal of Applied Phycology 2005, 17: 215-222 .
  • Vijayavel K, Anbuselvam C, Balasubramanian MP: Antioxidant Effect of the Marine Algae Chlorella vulgaris Against Naphthalene-induced Oxidative Stress in the Albino Rats, Molecular Cell Biochemistry 2007, 303: 39-44.
  • Wu L-C, Annie HJ-A, Shieh C-H, Lu I-W: Antioxidant and Antiproliferative Activities of Spirulina and Chlorella Water Extracts, Journal of Agricultural Food Chemistry 2005, 53: 4207-4212.
  • Sengul M, Yıldız H, Gungor N, Cetin B, Eser Z, Ercisli S: Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pakistan Journal of Pharmaceutical Sciences 2009, 1: 102-106.

Details

Primary Language English
Journal Section Research Article
Authors

Fethiye Ferda YILMAZ This is me
Ege University, Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Bornova, 35100 Izmir, Turkey


Zeliha DEMİREL
Ege University, Faculty of Engineering, Department of Bioengineering, Bornova, 35100 Izmir, Turkey
0000-0003-3675-7315


Nefise Ulku KARABAY-YAVASOGLU
Ege University, Faculty of Science, Department of Biology, Bornova, 35100 Izmir, Turkey


Guven OZDEMİR This is me
Ege University, Faculty of Science, Department of Biology, Bornova, 35100 Izmir, Turkey


Meltem CONK-DALAY
Ege University, Faculty of Engineering, Department of Bioengineering, Bornova, 35100 Izmir, Turkey

Publication Date January 1, 2017
Published in Issue Year 2017, Volume , Issue 1

Cite

APA Yılmaz, F. F. , Demirel, Z. , Karabay-yavasoglu, N. U. , Ozdemir, G. & Conk-dalay, M. (2017). Antimicrobial and Antioxidant Activities of Porphyridium cruentum . Hacettepe University Journal of the Faculty of Pharmacy , (1) , 1-7 . Retrieved from https://dergipark.org.tr/en/pub/hujpharm/issue/49754/638380