Bazı Bidens Türlerinin Antimikrobiyal Aktivitesi

Year 2016, Issue: 2, 129 - 134, 01.06.2016

Ülkü Gökçen İçöz Müjde Eryılmaz , Ayşe Nur Yazgan Betül Sever Yılmaz Mehmet Levent Altun

Abstract

Bu çalışmanın amacı, bazı Bidens türlerinin topraküstü kısımlarının, sulu ve etanollü ekstrelerinin antimikrobiyal aktivitelerinin araştırılmasıdır. Bidens cernua L. var. radiata DC., B. frondosa L. ve B. tripartita L. türlerinin Staphylococcus aureus ATCC 25923, S. aureus ATCC 43300 metisilin dirençli S. aureus , Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 ve Candida albicans ATCC 10231’a karşı antimikrobiyal aktiviteleri makrodilüsyon yöntemi ile belirlenmiştir. Çalışmada kullanılan türlerin etanollü ekstrelerinin, sulu ekstrelere kıyasla daha iyi antimikrobiyal aktivite gösterdiği belirlenmiştir. Etanollü ekstrelerin test mikroorganizmalarına karşı MIC değerleri 0.125-0.250 mg/ml olarak saptanırken, sulu ekstrelerin antibakteriyel etki göstermediği görülmüştür. B. frondosa ve B. tripartita’nın C. albicans’a karşı MİK değerleri sırasıyla, 0.500-1.000 mg/ml olarak saptanmıştır. Geleneksel tıpta Bidens türlerinin çeşitli cilt hastalıklarının ve yaralanmalarının tedavisinde ve ayrıca soğuk algınlığında kullanılması, antimikrobiyal etkisinin varlığı ile ilişkilendirilebilir. Bu çalışma Türkiye’de yetişen Bidens türleri ile yapılan ilk antimikrobiyal aktivite çalışmasıdır.

Keywords

Antimikrobiyal aktivite, Bidens sp., B. cernua var. radiata, B. frondosa, B. tripartita

Antimicrobial Activity of Some Bidens Species

Abstract

The aim of this study was to determine the antimicrobial activities of aerial parts extracts of some Bidens species against some bacteria and yeast. Aqueous and ethanolic aerial parts extracts of Bidens cernua L. var. radiata DC., B. frondosa L. and B. tripartita L. were screened for their antimicrobial activities against Staphylococcus aureus ATCC 25923, S. aureus ATCC 43300 methicillin-resistant S. aureus , Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231. Broth macrodilution method was used to determine the antimicrobial activities of plant extracts. Ethanolic extracts of tested species exhibited better antimicrobial activity than aqueous extracts. Ethanolic extracts of tested species possessed activity having MIC values of 0.125-0.250 mg/ ml against the tested microorganisms. No antibacterial activity was observed for all the aqueous extracts. Only aqueous extracts of B. frondosa and B. tripartita showed antifungal activity having MIC values of 0.500-1.000 mg/ml against C. albicans, respectively. It is conceivable that use of Bidens species for treatment of various skin diseases and injuries, and also cold in folk medicine is because of its antimicrobial activity. This is the first research on antimicrobial activity of Turkish Bidens species.

Keywords

Antimicrobial activity, Bidens sp., B. cernua var. radiata, B. frondosa, B. tripartita

References

• Connelly P. Horrible weed or miracle herb? A review of Bidens pilosa. Journal of the Australian Traditional-Medicine Society 2009, 15: 77-79.
• Korkmaz H, Mumcu Ü, Alkan S. A new alien species record for the flora of Turkey: Bidens bipinnata (Asteraceae). Biological Diversity and Conservation 2011, 4: 49-54.
• Chalchat JC, Petrovic S, Maksimovic Z, Gorunovic M. Composition of essential oil of Bidens cernua L., Asteraceae from Serbia. Journal of Essential Oil Research 2009, 21: 41-42.
• Brandao MGL, Krettli AU, Soares LSR, Nery CGC, Marinuzzi HC. Antimalarial activity of extracts and fractions from Bidens pilosa and other Bidens species (Asteraceae) correlated with the presence of acetylene and flavonoid compounds. Journal of Ethnopharmacology 1997, 57: 131-138.
• Chang MH, Wnag GJ, Kuo YH, Lee CK. The low polar constituents from Bidens pilosa L. var. minor (Blume) Sherff. Journal of the Chinese Chemical Society 2000, 47: 1131-1136.
• Redl K, Davis B, Bauer R. Chalcone glycosides from Bidens campylotheca. Phytochemistry 1993, 32: 218-220.
• Ubillas RP, Mendez CD, Jolad SD, Luo J, King SR, Carlson TJ, Fort DM. Antihyperglycemic acetylenic glucosides from Bidens pilosa. Planta Medica 2000, 66: 82-83.
• Wang J, Yang H, Lin ZW, Sun HD. Flavonoids from Bidens pilosa var. radiata. Phytochemistry 1997a, 46: 1275-1278.
• Wang J, Yang H, Lin ZW, Sun HD. Five new flavonoids from Bidens pilosa L. var. radiata SCH- BIP. Chinese Chemical Letters 1997b, 8: 599-602.
• Tomczykowa M, Tomczyk M, Jakoniuk P, Tryniszewska E. Antimicrobial and antifungal activities of the extracts and the essential oils of Bidens tripartita. Folia Histochememica Cytobiologica 2008, 46: 389-393.
• Diego FCR, Chagas-Paula DA, Da Costa FB, Souza CRF, Oliveira WP. Bioactive compounds in Bidens pilosa L. populations: a key step in the standardization of phytopharmaceutical preparations. Revista Brasileira de Farmacognosia 2013, 23: 28-35.
• Alarcon-Aguilara FJ, Roman-Ramos R, Perez-Gutierrez S, Aguilar-Contreras A, Contreras-Weber CC, Flores-Saenz JL. Study of the anti-hyperglycemic effect of plants used as antidiabetics. Journal of Ethnopharmacology 1998, 61: 101-110.
• Chang JS, Chiang LC, Chen CC, Liu LT, Wang KC, Lin CC. Antileukemic activity of Bidens pilosa L. var. minor (Blume) Sherff and Houttuynia cordata Thunb. American Journal of Chinese Medicine 2001, 29: 303-312.
• Wang N, Yao X, Ishii R, Kitanaka S. Bioactive sucrose esters from Bidens parviflora. Phytochemistry 2003, 62: 741-746.
• CLSI (Clinical and Laboratory Standards Institute). 2009. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, Approved standard. In: CLSI Publication M07-A8, eighth ed. Clinical and Laboratory Standards Institute, Wayne, PA.
• Karunai-Raj M, Balachandran C, Duralpandiyan V, Agastian P, Ignacımuthu S. Antimicrobial activity of Ulopterol isolated from Toddalia asiatica (L.) Lam: A traditional medicinal plant. Journal of Ethnopharmacology 2012, 140: 161-165.
• Rybalchenko NP, Prykhodko VA, Nagorna SS, Volynets NN, Ostapchuk AN, Klochko VV, Rybalchenko TV, Avdeeva LV. In vitro antifungal activity of phenylheptatriyne from Bidens cernua L. against yeasts. Fitoterapia 2010, 81: 336-338.
• Rahman A., Bajpai VK, Dung NT, Kang SC. Antibacterial and antioxidant activities of the essential oil and methanol extracts of Bidens frondosa Linn. International Journal of Food Science & Technology 2011, 46: 1238-1244.
• WHO Monographs on medicinal plants commonly used in the Newly Independent States (NIS). 2010. World Health Organization Press.
• Silva JJ, Cerdeira CD, Chavasco JM, Cintra ABP, Silva CBP, Mendonça AN, Ishikawa T, Boriollo MF, Chavasco JK. In vitro screening antibacterial activity of Bidens pilosa Linné and Annona crassiflora Mart. against oxacillin resistant Staphylococcus aureus (ORSA) from the aerial environment at the dental clinic. Revista do Instituto de Medicina Tropical de Sao Paulo 2014, 56: 333-340.
• Ashafa AOT, Afolayan AJ. Screening the root extracts from Bidens pilosa var. radiata (Asteraceae) for antimicrobial potentials. Journal of Medicinal Plants Research 2009, 3: 568-572.
• Adedapo A, Jimoh F, Afolayan A. Comparison of the nutritive value and biological activities of the acetone, methanol and water extracts of the leaves of Bidens pilosa and Chenopodium album. Acta Poloniae Pharmaceutica 2011, 68: 83-92.