Paeoniflorin Farede Maksimal Elektroşok ve PTZ Nöbetlerini Azaltır

Year 2012, Issue: 1, 17 - 30, 01.01.2012

Semra Koyunoğlu Okan Arıhan Yıldırım Sara Rüştü Onur Sedef Kır İhsan Çalış

Abstract

Paeonia türleri halk tıbbında epilepsinin tedavisi amacıyla kullanılan türlerdir, ancak Paeonia türlerinin ana etken maddesi olan paeoniflorinin etkileri bilimsel olarak incelenmemiştir. Bu çalışmada paeoniflorinin Maksimal Elektroşok MES ve pentilentetrazol PTZ ile oluşurulan jeneralize tonik-klonik konvülsiyon modelindeki etkileri araştırılmıştır. MES testinde paeoniflorin 100, 250 ve 500 mg/kg dozlarında intraperitoneal yolla verilmiştir. MES eşik testi ile hayvanların yüzde ellisinde konvülsiyona neden olan akım ölçülmüştür. Paeoniflorinin konvülsiyonlar üzerine etkileri once MES testi ile değerlendirilmiştir. Paeoniflorin 250 ve 500 mg/kg dozlarında antikonvülsif etki göstermiş ve konvülsiyona giren hayvan sayısını azaltmıştır. Paeoniflorinin antikonvülsif etkileri enjeksiyondan 90 dakika sonra gözlenmiştir. PTZ testinde paeoniflorinin 100 ve 250 mg/kg dozlarında jeneralize konvülsiyonlar için gereken süreyi ve konvülsiyon süresini doza bağımlı olarak uzatmıştır. Bu bulgular ışığında, paeoniflorinin MES’le indüklenen jeneralize tonik-klonik konvülsiyonları inhibe ettiğini değerlendirdik. Paeoniflorin ve paeonia türlerinde bulunan diğer monoterpenik glikozitler yeni antikonvülsif ilaçların geliştirilmesi için aday olarak değerlendirilebilir.

Keywords

Şakayık, Paeonia, paeoniflorin, maksimal elektroşok, PTZ, antikonvülsif

Paeoniflorin Diminishes Maximal Electroshock - and PTZ - induced Convulsions in Mice

Abstract

Paeonia species have been used to treat epilepsia in traditional medicine, however the effects of paeoniflorin, the main constituent of Paeonia species have not been studied scientifically. We studied the effects of paeoniflorin with generalized tonic-clonic convulsion model induced by Maximal Electroshock MES and pentylenetetrazolium PTZ in mice. In MES test paeoniflorin was given intraperitoneally at doses of 100, 250 and 500 mg/kg. The current strength which yielded convulsions in 50 % of the animals was calculated by MES threshold test. The effects of paeoniflorin on convulsions were evaluated firstly via MES test. Paeoniflorin exerted anticonvulsive effect at 250 and 500 mg/kg and decreased the number of animals having convulsion. Anticonvulsive effect of paeoniflorin was observed 90 min after injection. In PTZ test we observed that 100 and 250 mg/kg paeoniflorin prolongs both the time period required for generalized convulsions and the duration in a dose dependent fashion. Based on these findings, we conclude that paeoniflorin inhibited generalized tonic-clonic convulsions induced by MES, and partially attenuated seizures in absence seizure model. Paeoniflorin and other monoterpenic glycosides of Paeonia species may be considered as a candidate for the development of a new anticonvulsive drug.

Keywords

Paeony, Paeonia, paeoniflorin, maximal electroshock, PTZ, antiseizure

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