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Tek-Donör Plazmasından Hazırlanan Fibrin Glue’nun Sıçanlarda Yara İyileşmesi Üzerine Etkisinin İncelenmesi

Year 2009, Issue: 2, 83 - 93, 01.06.2009

Abstract

Ticari fibrin yapıştırıcılar, insan plazma havuzlarından elde edilmektedir, Fibrin yapıştırıcı günümüzde klinik kullanım için onaylanmıştır. Tek-donör plazmasından hazırlanan fibrin glue viral enfeksiyonların bulaşma riskini önlemeden dolayı önemli bir avantaja sahiptir. Bu çalışmada, etanol çöktürmesiyle yeterli miktarda konsantre fibrinojen hazırlanmış ve yüksek verimlilikte 79% elde edilmiştir. Ayrıca, faktör XIII varlığı 5M üre çözeltisinde gösterilmiştir. Wistar Albino sıçanların n=15 vertebralarının her iki yanına deriye 5’er cm uzunluğunda tam-kalınlıklı iki adet paralel paravertebral insizyon yapılmıştır. Her bir hayvanın insizyon yaralarından biri fibrin glue ile tedavi edilmiştir. İyileşme süreci postoperatif 2,7 ve 14. günlerde histolojik olarak incelenmiştir. Tüm grupların fibrin glue ile tedavi edilen yaralarında hücre proliferasyon ve kolajen oluşumunun önemli derecede artmış olduğu gözlenmiştir. Fibrin glue, yara iyileşmesi üzerine artmış granülasyon ve reepitelizasyon şeklinde olumlu etki göstermiştir.

References

  • 1. Spotnitz WD. Fibrin sealant: past, present, and future: a brief review: World J Surg. 34: 632-634 (2010).
  • 2. Tawes RL, Sydorak GR, DuVall TB. Autologous fibrin glue: The last step in operative hemostasis. Am J Surg. 168: 120-122 (1994).
  • 3. Martinowitz U, Saltz R. Fibrin sealant. Curr Opin Hematol. 3: 395-402 (1996).
  • 4. Epstein GH, Weisman RA, Zwillenberg S, Schreiber AD. A new autologous fibrinogenbased adhesive for otologic surgery. Ann Otol Rhinol Laryngol. 195: 40-45 (1986).
  • 5. Radosevich M, Goubran HI, Bunouf T. Fibrin sealant: scientific rationale, production methods, properties, and current clinical use. Vox Sang. 72: 133-143 (1997).
  • 6. Siedentop KH, Chung SE, Park JJ, Sanchez B, Bhatttacharya T, Marx G. Evaluation of pooled fibrin sealant for ear surgery. Am J Otol. 18: 660-664 (1997).
  • 7. Sims CD, Butler PE, Cao YL, Casanova R, Randolph MA, Black A, Vacanti CA, Yaremchuk MJ. Tissue engineered neocartilage using plasma derived polymer substrates and chondrocytes. Plast Reconstr Surg. 101: 1580-1585 (1998).
  • 8. Fraioli B, Pastore FS, Floris R, Vagnozzi R, Simonetti G, Liccardo G, Giuffre R. Computed tomography-guided transsphenoidal closure of postsurgical cerebrospinal fluid fistula: a transmucosal needle technique. Surg Neurol. 48: 409-412 (1997).
  • 9. Moore MM, Nguyen DH, Spotnitz WD. Fibrin sealant reduces serous drainage and allows for earlier drain removal after axilary dissection: a randomized prospective trial. Am Surg. 63: 97-102 (1997).
  • 10. Kollias SL, Fox JM. Meniscal repair. Where do we go from here? Clin Sports Med.15: 621-630 (1996).
  • 11. Evrard VA, De Bellis A, Boeckx W, Brosens IA. Peritoneal healing after fibrin glue application: A comparative study in a rat model. Hum Repord. 11: 1877-1880 (1996).
  • 12. Trombelli L, Scabbia A, Scapoli C, Calura G. Clinical effect of tetracycline demineralization and fibrin-fibronectin sealing system application on healing response following flap debridement surgery. J Periodontol. 67: 688-693 (1996).
  • 13. Trombelli L, Scabbia A, Wikesjo UM, Calura G. Fibrin glue application in conjunction with tetracycline root conditioning and coronally positioned flap procedure in the treatment of human gingival recession defects. J Clin Periodontol. 23: 861-867 (1996).
  • 14. Lorand L. Fibrin clots. Nature. 166: 694-696 (1950).
  • 15. Li Y, Bao Y, Jiang T, Tan L, Gao Y, Li J. Effect of the combination of fibrin glue and growth hormone on incomplete intestinal anastomoses in a rat model of intraabdominal sepsis. J Surg Res. 131: 111-117 (2006).
  • 16. Spector JG. Neural repair in facial paralysis: Clinical and experimental studies. Eur Arch Otorhinolaryngol. 1:68-75 (1997).
  • 17. Dresdale A, Rose EA, Jeevanandam V, Reemtsma K, Bowman FO, Malm JR. Preparation of fibrin glue from single-donor fresh-frozen plasma. Surgery. 97: 750-754 (1985).
  • 18. Inaba S. Clinical usefulness of autologous transfusion-history, methodologies and problems with popularization. Nippon Rinsho. 55: 1861-1870 (1997).
  • 19. Laidmäe I, McCormick ME, Herod JL, Pastore JJ, Salum T, Sawyer ES, Janmey PA, Uibo R. Stability, sterility, coagulation, and immunologic studies of salmon coagulation proteins with potential use for mammalian wound healing and cell engineering. Biomaterials. 27:5771 (2006).
  • 20. Moon SP, Il Cha C. Biochemical aspects of autologous fibrin glue derived from ammonium sulphate precipitation. Laryngoscope. 103: 193-196 (1993).
  • 21. Weis-Fogh US. Fibrinogen prepared from small blood samples for autologous use in a tissue adhesive system. Eur Surg Res. 20: 381-389 (1988).
  • 22. Weisman RA, Torsiglieri AJ, Schreiber AD, Epstein PA. Biochemical characterization of autologous fibrinogen adhesiv. Laryngoscope. 97: 1186-1190 (1987).
  • 23. Kato Y, Yamataka A, Miyano G, Tei E, Koga H, Lane GJ, Miyano T. Tissue adhesives for repairing inguinal hernia: a preliminary study. J Laparoendosc Adv Surg Tech A. 15:424-8 (2005).
  • 24. Kjaergard HK, Weis-Fogh US, Sorensen H, Thiis J, Rygg I. A simple method of preparation of autologous fibrin glue by means of ethanol. Surg Gynecol Obstet. 175: 72-73 (1992).
  • 25. Kanellos I, Christoforidis E, Kanellos D, Pramateftakis MG, Sakkas L, Betsis D. The healing of colon anastomosis covered with fibrin glue after early postoperative intraperitoneal chemotherapy. Tech Coloproctol. 10: 115-20 (2006).
  • 26. Park JJ, Siedontop KH, Chung S, Sanchez B, Bhattacharyya T. Comparison of the bonding power of various autologous fibrin tissue adhesives. Am J Otol. 18: 655-659 (1997).
  • 27. Kaftan H, Eyrich D, Schindler E, Göpferich A, Hosemann W. Fibrin gel for treatment of tympanic membrane perforations: an experimental animal model. HNO. 54: 943-6 (2006).
  • 28. Brown LF, Lanir N, Mcdonagh J, Tognazzi K, Dvorak AM, Dvorak HF. Fibroblast migration in fibrin gel matrices. Am J Pathol. 142: 273-283 (1993).
  • 29. Michel D, Harmand M-F. Fibrin seal in wound healing: Effect of thrombin and [Ca2+] on human skin fibroblast growth and collagen production. J Dermatol Science. 1: 325- 334 (1990).
  • 30. Hashimoto J, Kurosaka M, Yoshiya S, Hirohata K. Meniscal repair using fibrin sealant and endothelial cell growth factor. Am J Sports Medicine. 20(5): 537-541 (1992).
  • 31. Stanojkovi Z, Stanojevi G, Stojanovi M, Mili D, Zivi S. Determination of fibrin glue with antibiotics on collagen production in colon anastomosis. Vojnosanit Pregl. 65: 681-7 (2008).
  • 32. Liu SA, Tung KC, Cheng CC, Chiu YT. The impact of different closure materials on pharyngeal wound healing: an experimental animal study. Eur Arch Otorhinolaryngol. 265: 227-31 (2008).
  • 33. Yazici A, Naiboglu B, Oysu C, Zer Toros S, Noseri H, Tepe Karaca C, Egeli E. Effect of ototopical ciprofloxacin-dexamethasone on myringotomy in a rat model. Int J Pediatr Otorhinolaryngol. 73: 301-5 (2009).
  • 34. Li Y, Bao Y, Jiang T, Tan L, Liu F, Li J. Combination of fibrin glue with growth hormone augments healing of incomplete intestinal anastomoses in a rat model of intraabdominal sepsis: a dynamic study. J Invest Surg. 20: 301-6 (2007).
  • 35. Akgül T, Ayyildiz A, Cebeci O, Nuhoğlu B, Ozer E, Germiyanoğlu C, Ustün H. Effect of cyanoacrylic glue on penile fracture: an experimental study. J Urol. 180: 749-52 (2008).

Evaluation of the Effect of Fibrin Glue Prepared from Single-Donor Plasma on Wound Healing in Rats

Year 2009, Issue: 2, 83 - 93, 01.06.2009

Abstract

Commercial fibrin sealant products derived from pooled human plasma. Fibrin sealant has been approved for clinical use today. Fibrin glue prepared from single donor plasma has the advantage of safety from transmission of viral infections. Sufficient quantity of concentrated fibrinogen was produced by ethanol precipitation and obtained in a high percentage of recovery 79% . Additionally, presence of factor XlII was indicated in 5M urea solution. Two full-thickness, 5 cm-long, paravertebral incisions were made through the skin, on each side of the vertebral columns of the 15 Wistar Albino rats. One of the incisional wounds of each animal was treated with fibrin glue. The other incisional wound was not treated and acted as a control. The healing process was examined histologically at postoperative 2nd, 7th and 14th days. Cell proliferation and collagen formation were found to be significantly increased on the fibrin glue-treated sites of all groups. Fibrin glue revealed a positive effect on wound healing observed as enhanced granulation and reepithelization.

References

  • 1. Spotnitz WD. Fibrin sealant: past, present, and future: a brief review: World J Surg. 34: 632-634 (2010).
  • 2. Tawes RL, Sydorak GR, DuVall TB. Autologous fibrin glue: The last step in operative hemostasis. Am J Surg. 168: 120-122 (1994).
  • 3. Martinowitz U, Saltz R. Fibrin sealant. Curr Opin Hematol. 3: 395-402 (1996).
  • 4. Epstein GH, Weisman RA, Zwillenberg S, Schreiber AD. A new autologous fibrinogenbased adhesive for otologic surgery. Ann Otol Rhinol Laryngol. 195: 40-45 (1986).
  • 5. Radosevich M, Goubran HI, Bunouf T. Fibrin sealant: scientific rationale, production methods, properties, and current clinical use. Vox Sang. 72: 133-143 (1997).
  • 6. Siedentop KH, Chung SE, Park JJ, Sanchez B, Bhatttacharya T, Marx G. Evaluation of pooled fibrin sealant for ear surgery. Am J Otol. 18: 660-664 (1997).
  • 7. Sims CD, Butler PE, Cao YL, Casanova R, Randolph MA, Black A, Vacanti CA, Yaremchuk MJ. Tissue engineered neocartilage using plasma derived polymer substrates and chondrocytes. Plast Reconstr Surg. 101: 1580-1585 (1998).
  • 8. Fraioli B, Pastore FS, Floris R, Vagnozzi R, Simonetti G, Liccardo G, Giuffre R. Computed tomography-guided transsphenoidal closure of postsurgical cerebrospinal fluid fistula: a transmucosal needle technique. Surg Neurol. 48: 409-412 (1997).
  • 9. Moore MM, Nguyen DH, Spotnitz WD. Fibrin sealant reduces serous drainage and allows for earlier drain removal after axilary dissection: a randomized prospective trial. Am Surg. 63: 97-102 (1997).
  • 10. Kollias SL, Fox JM. Meniscal repair. Where do we go from here? Clin Sports Med.15: 621-630 (1996).
  • 11. Evrard VA, De Bellis A, Boeckx W, Brosens IA. Peritoneal healing after fibrin glue application: A comparative study in a rat model. Hum Repord. 11: 1877-1880 (1996).
  • 12. Trombelli L, Scabbia A, Scapoli C, Calura G. Clinical effect of tetracycline demineralization and fibrin-fibronectin sealing system application on healing response following flap debridement surgery. J Periodontol. 67: 688-693 (1996).
  • 13. Trombelli L, Scabbia A, Wikesjo UM, Calura G. Fibrin glue application in conjunction with tetracycline root conditioning and coronally positioned flap procedure in the treatment of human gingival recession defects. J Clin Periodontol. 23: 861-867 (1996).
  • 14. Lorand L. Fibrin clots. Nature. 166: 694-696 (1950).
  • 15. Li Y, Bao Y, Jiang T, Tan L, Gao Y, Li J. Effect of the combination of fibrin glue and growth hormone on incomplete intestinal anastomoses in a rat model of intraabdominal sepsis. J Surg Res. 131: 111-117 (2006).
  • 16. Spector JG. Neural repair in facial paralysis: Clinical and experimental studies. Eur Arch Otorhinolaryngol. 1:68-75 (1997).
  • 17. Dresdale A, Rose EA, Jeevanandam V, Reemtsma K, Bowman FO, Malm JR. Preparation of fibrin glue from single-donor fresh-frozen plasma. Surgery. 97: 750-754 (1985).
  • 18. Inaba S. Clinical usefulness of autologous transfusion-history, methodologies and problems with popularization. Nippon Rinsho. 55: 1861-1870 (1997).
  • 19. Laidmäe I, McCormick ME, Herod JL, Pastore JJ, Salum T, Sawyer ES, Janmey PA, Uibo R. Stability, sterility, coagulation, and immunologic studies of salmon coagulation proteins with potential use for mammalian wound healing and cell engineering. Biomaterials. 27:5771 (2006).
  • 20. Moon SP, Il Cha C. Biochemical aspects of autologous fibrin glue derived from ammonium sulphate precipitation. Laryngoscope. 103: 193-196 (1993).
  • 21. Weis-Fogh US. Fibrinogen prepared from small blood samples for autologous use in a tissue adhesive system. Eur Surg Res. 20: 381-389 (1988).
  • 22. Weisman RA, Torsiglieri AJ, Schreiber AD, Epstein PA. Biochemical characterization of autologous fibrinogen adhesiv. Laryngoscope. 97: 1186-1190 (1987).
  • 23. Kato Y, Yamataka A, Miyano G, Tei E, Koga H, Lane GJ, Miyano T. Tissue adhesives for repairing inguinal hernia: a preliminary study. J Laparoendosc Adv Surg Tech A. 15:424-8 (2005).
  • 24. Kjaergard HK, Weis-Fogh US, Sorensen H, Thiis J, Rygg I. A simple method of preparation of autologous fibrin glue by means of ethanol. Surg Gynecol Obstet. 175: 72-73 (1992).
  • 25. Kanellos I, Christoforidis E, Kanellos D, Pramateftakis MG, Sakkas L, Betsis D. The healing of colon anastomosis covered with fibrin glue after early postoperative intraperitoneal chemotherapy. Tech Coloproctol. 10: 115-20 (2006).
  • 26. Park JJ, Siedontop KH, Chung S, Sanchez B, Bhattacharyya T. Comparison of the bonding power of various autologous fibrin tissue adhesives. Am J Otol. 18: 655-659 (1997).
  • 27. Kaftan H, Eyrich D, Schindler E, Göpferich A, Hosemann W. Fibrin gel for treatment of tympanic membrane perforations: an experimental animal model. HNO. 54: 943-6 (2006).
  • 28. Brown LF, Lanir N, Mcdonagh J, Tognazzi K, Dvorak AM, Dvorak HF. Fibroblast migration in fibrin gel matrices. Am J Pathol. 142: 273-283 (1993).
  • 29. Michel D, Harmand M-F. Fibrin seal in wound healing: Effect of thrombin and [Ca2+] on human skin fibroblast growth and collagen production. J Dermatol Science. 1: 325- 334 (1990).
  • 30. Hashimoto J, Kurosaka M, Yoshiya S, Hirohata K. Meniscal repair using fibrin sealant and endothelial cell growth factor. Am J Sports Medicine. 20(5): 537-541 (1992).
  • 31. Stanojkovi Z, Stanojevi G, Stojanovi M, Mili D, Zivi S. Determination of fibrin glue with antibiotics on collagen production in colon anastomosis. Vojnosanit Pregl. 65: 681-7 (2008).
  • 32. Liu SA, Tung KC, Cheng CC, Chiu YT. The impact of different closure materials on pharyngeal wound healing: an experimental animal study. Eur Arch Otorhinolaryngol. 265: 227-31 (2008).
  • 33. Yazici A, Naiboglu B, Oysu C, Zer Toros S, Noseri H, Tepe Karaca C, Egeli E. Effect of ototopical ciprofloxacin-dexamethasone on myringotomy in a rat model. Int J Pediatr Otorhinolaryngol. 73: 301-5 (2009).
  • 34. Li Y, Bao Y, Jiang T, Tan L, Liu F, Li J. Combination of fibrin glue with growth hormone augments healing of incomplete intestinal anastomoses in a rat model of intraabdominal sepsis: a dynamic study. J Invest Surg. 20: 301-6 (2007).
  • 35. Akgül T, Ayyildiz A, Cebeci O, Nuhoğlu B, Ozer E, Germiyanoğlu C, Ustün H. Effect of cyanoacrylic glue on penile fracture: an experimental study. J Urol. 180: 749-52 (2008).
There are 35 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Halil Aksoy

Şule Apikoğlu-rabus This is me

Fikriye Uras This is me

Nejat Savcı This is me

F. Canan Alatlı This is me

Gülçin Erseven This is me

Nesrin Emekli This is me

Publication Date June 1, 2009
Published in Issue Year 2009 Issue: 2

Cite

Vancouver Aksoy H, Apikoğlu-rabus Ş, Uras F, Savcı N, Alatlı FC, Erseven G, Emekli N. Evaluation of the Effect of Fibrin Glue Prepared from Single-Donor Plasma on Wound Healing in Rats. HUJPHARM. 2009(2):83-9.