Tek-Donör Plazmasından Hazırlanan Fibrin Glue’nun Sıçanlarda Yara İyileşmesi Üzerine Etkisinin İncelenmesi

Year 2009, Issue: 2, 83 - 93, 01.06.2009

Halil Aksoy , Şule Apikoğlu-rabus Fikriye Uras Nejat Savcı F. Canan Alatlı Gülçin Erseven Nesrin Emekli

Abstract

Ticari fibrin yapıştırıcılar, insan plazma havuzlarından elde edilmektedir, Fibrin yapıştırıcı günümüzde klinik kullanım için onaylanmıştır. Tek-donör plazmasından hazırlanan fibrin glue viral enfeksiyonların bulaşma riskini önlemeden dolayı önemli bir avantaja sahiptir. Bu çalışmada, etanol çöktürmesiyle yeterli miktarda konsantre fibrinojen hazırlanmış ve yüksek verimlilikte 79% elde edilmiştir. Ayrıca, faktör XIII varlığı 5M üre çözeltisinde gösterilmiştir. Wistar Albino sıçanların n=15 vertebralarının her iki yanına deriye 5’er cm uzunluğunda tam-kalınlıklı iki adet paralel paravertebral insizyon yapılmıştır. Her bir hayvanın insizyon yaralarından biri fibrin glue ile tedavi edilmiştir. İyileşme süreci postoperatif 2,7 ve 14. günlerde histolojik olarak incelenmiştir. Tüm grupların fibrin glue ile tedavi edilen yaralarında hücre proliferasyon ve kolajen oluşumunun önemli derecede artmış olduğu gözlenmiştir. Fibrin glue, yara iyileşmesi üzerine artmış granülasyon ve reepitelizasyon şeklinde olumlu etki göstermiştir.

Keywords

Fibrin glue, yara iyileşmesi, sıçanlar

Evaluation of the Effect of Fibrin Glue Prepared from Single-Donor Plasma on Wound Healing in Rats

Abstract

Commercial fibrin sealant products derived from pooled human plasma. Fibrin sealant has been approved for clinical use today. Fibrin glue prepared from single donor plasma has the advantage of safety from transmission of viral infections. Sufficient quantity of concentrated fibrinogen was produced by ethanol precipitation and obtained in a high percentage of recovery 79% . Additionally, presence of factor XlII was indicated in 5M urea solution. Two full-thickness, 5 cm-long, paravertebral incisions were made through the skin, on each side of the vertebral columns of the 15 Wistar Albino rats. One of the incisional wounds of each animal was treated with fibrin glue. The other incisional wound was not treated and acted as a control. The healing process was examined histologically at postoperative 2nd, 7th and 14th days. Cell proliferation and collagen formation were found to be significantly increased on the fibrin glue-treated sites of all groups. Fibrin glue revealed a positive effect on wound healing observed as enhanced granulation and reepithelization.

Keywords

Fibrin glue, wound healing, rats

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